Name Hour Date For each of the following sequences, fill in either the DNA, the mRNA sequence, the rRNA anticodons, or the amino acid sequences that have been left blank. If several sequences might work choose any one. 1. DNA 2. TAC TGA TCG ACC CCC ATA ATG AAA ATC mRNA A U G tRNA UAC UGA UCG ACC CCC AUA AUG AAA AUC AA MET-THR-SER-TRP-GLY-TYR-TYR-PHE-STOP DNA T A C C G C T C C G C C G T C G A C A A T A C C A C T mRNA AUG GCG AGG CGG CAG CUG UUA UGG UGA tRNA UAC CGC UCC GCC GUC GAC AAU ACC ACU AA MET-ALA-ARG-ARG-GLU-LEU-LEU-TRP-STOP 3. DNA A C U A G C U G G G G G U A U U A C U U U U A G TAC CAC CCC CGT ATG GCT GGG AAT ATC mRNA AUG GUG GGG GCA UAC CGA CCC UUA UAG tRNA U A C AA MET-VAL-GLY-ALA-TRY-ARG-PRO-LEU-STOP C A C C C C C G U A U G G C U G G G A A U A U C GLY ( STOP) Highlighted parts for 4 and 5 will be different for different students… 4. DNA TAC GCA CCA AAA AAC TAC CAA GGT ATC mRNA AUG CGU GGU UUU UUC AUG GUO GGA UAG tRNA UAC GCA CCA AAA AAC UAC CAA GGU AUC AA MET 5. DNA ARG GLY PHE PHE MET T A C CTC ACA CTA CGA A T G TTG GGA ATT mRNA AUG GAG U G U tRNA UAC C U C ACA CUA CGA AUG U U G GGA A U U AA MET-GLY-CYS-ASP G A U GCU UAC AAC CCU UAA ALA TRY-ASN PRO- STOP VAL 6. What are the three differences between RNA and DNA? DNA RNA Deoxyribose sugar-no oxygen Ribose sugar-oxygen Double stranded Single stranded Thymine Uracil 7. Where is DNA found in the cell? nucleus Where is RNA found in the cell? Cytoplasm and nucleus 8. Name the three types of RNA and what they do. Messenger RNA (mRNA) carries the message that will be translated to form a protein. Ribosomal RNA (rRNA) forms part of ribosomes where proteins are made. Transfer RNA (tRNA) brings amino acids from the cytoplasm to a ribosome. 9. Draw an mRNA strand that is complementary to the DNA strand AATTGC. Circle a nucleotide. Etc…..make sure you have the phosphate and the ribose sugar… 10. What are the steps of transcription translation? 1. For translation to begin, tRNA binds to a start codon and signals the ribosome to assemble. A complementary tRNA molecule binds to the exposed codon, bringing its amino acid close to the first amino acid. 2. The ribosome helps form a polypeptide bond between the amino acids and breaks the bond between the first tRNA molecule and its amino acid. 3. The ribosome pulls the mRNA strand the length of one codon. The now empty tRNA molecule returns to the cytoplasm. A complementary tRNA molecule binds to the next exposed codon. Once the stop codon is reached, the ribosome releases the protein and disassembles.