File - Central Dogma of Molecular Biology
... DNA Replication • Primers are the short nucleotide fragments (DNA or RNA) with an available free 3’ end to which DNA polymerase III (DNA pol III) will add nucleotides according to the base paring rules. • Primase is the enzyme that starts an RNA chain from scratch creating a primer that can initiat ...
... DNA Replication • Primers are the short nucleotide fragments (DNA or RNA) with an available free 3’ end to which DNA polymerase III (DNA pol III) will add nucleotides according to the base paring rules. • Primase is the enzyme that starts an RNA chain from scratch creating a primer that can initiat ...
Biotechnology toolkit part 1 File
... This is done by mechanically breaking the cells open, then using detergents and enzymes to break down the cell walls and membranes. The detergents also break down the nuclear membrane releasing the DNA. Step 2: remove the unwanted cell debris This is done by filtering the extract or by centrifuging ...
... This is done by mechanically breaking the cells open, then using detergents and enzymes to break down the cell walls and membranes. The detergents also break down the nuclear membrane releasing the DNA. Step 2: remove the unwanted cell debris This is done by filtering the extract or by centrifuging ...
Restriction Enzymes
... Each enzyme recognized its own specific sequence of DNA bases. It is at this sequence that the DNA was cut. ...
... Each enzyme recognized its own specific sequence of DNA bases. It is at this sequence that the DNA was cut. ...
Chapters 16-17 (DNA and protein synthesis)
... - However, the amazing accuracy of DNA replication is actually 1 error in 10 billion nucleotides DNA polymerases check each newly added nucleotide against its template and remove incorrect nucleotides. - The likelihood of mistakes occurring is reduced because the enzyme DNA polymerase proofreads and ...
... - However, the amazing accuracy of DNA replication is actually 1 error in 10 billion nucleotides DNA polymerases check each newly added nucleotide against its template and remove incorrect nucleotides. - The likelihood of mistakes occurring is reduced because the enzyme DNA polymerase proofreads and ...
RNA
... collection of DNA. Is only performed once in the cell’s life. For reproduction where all the instructions for making an organism must be passed on. ...
... collection of DNA. Is only performed once in the cell’s life. For reproduction where all the instructions for making an organism must be passed on. ...
Extracting DNA Lab – S
... Name ______________________________ Period __________________ Date __________________ ...
... Name ______________________________ Period __________________ Date __________________ ...
Practice Exam 2
... How to Use this Practice Exam: I post practice exams to allow you to get a real sense of the experience of taking a Biology 200 exam. The best way to use each exam is as follows. 1. Do NOT answer the questions as a problem set. 2. Study material using your lecture and lab notes and do problem sets F ...
... How to Use this Practice Exam: I post practice exams to allow you to get a real sense of the experience of taking a Biology 200 exam. The best way to use each exam is as follows. 1. Do NOT answer the questions as a problem set. 2. Study material using your lecture and lab notes and do problem sets F ...
LATg Training Course - AZ Branch AALAS Homepage
... • DNA is a long string (polymer) of 4 bases • These bases universal! – A = Adenosine – T = Thymine – C = Cytosine – G = Guanine • The order (sequence) of the bases is what makes one gene different from another gene. ...
... • DNA is a long string (polymer) of 4 bases • These bases universal! – A = Adenosine – T = Thymine – C = Cytosine – G = Guanine • The order (sequence) of the bases is what makes one gene different from another gene. ...
Cloning and Sequencing
... Reading the Sequence • In the tube with the ddTTP, every time it is time to add a T to the new strand, some Ts will be dTTP and some will be ddTTP. • When the ddTTP is added, then extension stops and you have a DNA fragment of a particular length. • The T tube will, therefore, have a series of DNA ...
... Reading the Sequence • In the tube with the ddTTP, every time it is time to add a T to the new strand, some Ts will be dTTP and some will be ddTTP. • When the ddTTP is added, then extension stops and you have a DNA fragment of a particular length. • The T tube will, therefore, have a series of DNA ...
Slide 1
... • What is one difference between RNA and DNA? • secondary structure (double stranded helix vs. single stranded) • nitrogenous bases (RNA uses uracil instead of thyamine) ...
... • What is one difference between RNA and DNA? • secondary structure (double stranded helix vs. single stranded) • nitrogenous bases (RNA uses uracil instead of thyamine) ...
Chapter02 Nucleotides and Nucleic Acids(核酸化学)
... response to presence of radioactive isotopes in nucleic acid molecules DNA Replication DNA is a double-helical molecule Each strand of the helix must be copied in complementary fashion by DNA polymerase Each strand is a template for copying DNA polymerase requires template and primer Primer: an olig ...
... response to presence of radioactive isotopes in nucleic acid molecules DNA Replication DNA is a double-helical molecule Each strand of the helix must be copied in complementary fashion by DNA polymerase Each strand is a template for copying DNA polymerase requires template and primer Primer: an olig ...
DNA and Technology
... Not only can DNA fingerprinting be used to convict someone of a crime, it can also be used to show someone’s innocence as in the case of Dr. Sam ...
... Not only can DNA fingerprinting be used to convict someone of a crime, it can also be used to show someone’s innocence as in the case of Dr. Sam ...
DNA - E. R. Greenman
... • Something in the S bacteria was taken up by the R and used by them so that they became S and caused pneumonia • Some chemical changed the cells • Experiment showed this was a valid hypothesis ...
... • Something in the S bacteria was taken up by the R and used by them so that they became S and caused pneumonia • Some chemical changed the cells • Experiment showed this was a valid hypothesis ...
ExamView - Chap 13 Review Essay Short.tst
... into the DNA of other organisms. In selective breeding, organisms with desired traits are produced by selecting organisms for their traits and then mating, or crossing, them. Selective breeding does not directly change the DNA of living organisms. PTS: 1 NAT: C.2.a | E.2 STA: BL.5.c | BL.3.a KEY: an ...
... into the DNA of other organisms. In selective breeding, organisms with desired traits are produced by selecting organisms for their traits and then mating, or crossing, them. Selective breeding does not directly change the DNA of living organisms. PTS: 1 NAT: C.2.a | E.2 STA: BL.5.c | BL.3.a KEY: an ...
Replisome
The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.