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What is a DNA?
What is a DNA?

... are cellular organelles, contain their own DNA ...
Exam IV 1710_1711 F'01.doc
Exam IV 1710_1711 F'01.doc

... The nucleotide sequence in DNA recognized by an RNA polymerase which indicates that this location is the start of a gene/operon (a transcription unit if you prefer) and to which the RNA polymerase binds is called a/an: a. ...
The biomolecules of terrestrial life
The biomolecules of terrestrial life

... Chiral molecules are isomers with a center of symmetry ( stereocenter ) They cannot be superimposed to their mirror image The two mirror images of a chiral molecule are called enantiomers Amino acids are chiral The carbon atom at the center of the amino acid is the stereocenter ...
When replication travels on damaged templates: bumps and blocks
When replication travels on damaged templates: bumps and blocks

... the specificity associated with DNA glycosylases for their respective structural lesions. Overall however, the observations that UV survival and the recovery of replication are not severely impaired in the absence of any or all of these inducible polymerases imply that these enzymes are not essentia ...
PowerPoint
PowerPoint

... flanking regions where PCR primers bind are constant Homozygote = both alleles are the same length Heterozygote = alleles differ and can be resolved from one another ...
DNA Structure
DNA Structure

File
File

... 2. The complementary bases are added to each template strand. 3. The 2 new strands are proofread for errors. When a cell copies its DNA (replication), the original DNA ladder is broken apart and new nucleotides are added to the center. This creates two exact copies, each one made from half the origi ...
Chapter 2
Chapter 2

... AAUAAA sequence after last codon is a signal for an enzyme to cut the pre-mRNA; then another enzyme adds 100 to 300 adenines—the “tail.” May assist in export from nucleus; important for stability of mRNA. ...
Basic Molecular Theory - American Society of Cytopathology
Basic Molecular Theory - American Society of Cytopathology

... • Strand splitting is mediated by 2 DNA enzymes: – Topoisomerase II (gyrase): removes supercoiling of DNA by  creating transitory brakes in the sugar‐phosphate  backbone – Helicase: unwinds the DNA helix into single strands,  facilitating replication, by breaking hydrogen bonds ...
Genetic Technology - Solon City Schools
Genetic Technology - Solon City Schools

...  What macromolecule do you think they are made of?  They are PROTEINS that cut ...
Gene%20Sequencing[2]
Gene%20Sequencing[2]

... Double-stranded molecule; covalent bonds between ribose/phosphate backbone on outside; hydrogen bonds between nitrogen bases on inside ...
Strawberry DNA Extraction Lab
Strawberry DNA Extraction Lab

BioH From DNA to proteins
BioH From DNA to proteins

...  Helicase – unwind & start strand separation  RNA polymerase – brings complementary base-matching nucleotides  Ligase – corrections and gap corrections • Promoter sequence on mRNA - signals “start” for transcribing DNA sequence into RNA sequence • ONE strand only – forming juvenile RNA • Uracil u ...
dna technology and genomics
dna technology and genomics

... 2) Describe the natural function of restriction enzymes and explain how they are used in recombinant DNA technology. 3) Explain how the creation of sticky ends by restriction enzymes is useful in producing a recombinant DNA molecule. 4) Describe techniques that allow identification of recombinant ce ...
dna and its structure
dna and its structure

Section 4
Section 4

LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

... c) BHC 18. Distinguish glucose and fructose by oxidation and reduction reactions. 19. Explain the isolation of terpenes. 20. Discuss the different types of soil. 21. Describe the importance of flavones and flavanoids. 22. Give any two methods of preparation of an amino acid. PART C Answer any four q ...
Gene Mutation, DNA Repair, and Transposition
Gene Mutation, DNA Repair, and Transposition

... Other chemicals can have other effects o Oxidative reactions can damage the DNA, causing chemical changes to the bases and leading to mutations o Intercalating agents are ring-containing molecules that are about the size of a base pair  These produce frameshift mutations o Radiation o UV radiation ...
Week 3 Pre-Lecture Slides
Week 3 Pre-Lecture Slides

... •  What would happen to transcription if the -10 and -35 boxes were switched? What if the +1 was a different base? What if the termination sequence was lost? •  There are four channels in the RNA polymerase protein leading from the core to the outside. Name each of these channels usefully based on t ...
Applied molecular technique
Applied molecular technique

... degrades the peptidoglycan layer of the cell wall. A successive treatment with detergent dissolves the lipids of the cell membrane. Chelating agents, such as EDTA (ethylene diamine tetraacetate), are also used, especially with gram-negative bacteria, to remove the metal ions that bind components of ...
Protein Synthesis Project
Protein Synthesis Project

... 8. How many amino acids does this complete protein contain? _____________ 9. This protein is called pro-insulin. In order for it to operate in the body, a segment between #30 and #66 amino acids must be removed. The remaining sections are reconnected to form insulin. How many amino acids are there i ...
Information Transfer and Protein Synthesis The DNA
Information Transfer and Protein Synthesis The DNA

... b. Introns are removed before the RNA leaves the nucleus 2. Exons a. Regions that will be translated into protein ...
Protein Synthesis Lab 2016 - Liberty Union High School District
Protein Synthesis Lab 2016 - Liberty Union High School District

... information from DNA must be transmitted from the nucleus to the cytoplasm. During! transcription, each gene on the DNA is read and codes directly for a messenger RNA! (mRNA) molecule. The mRNA is made by matching its complementary bases — C, G, A, and U! (uracil) — to the DNA bases. This process is ...
Note 8.2 - DNA Sequencing
Note 8.2 - DNA Sequencing

RNA and transcription
RNA and transcription

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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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