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Plasmids and DNA Digestion
Plasmids and DNA Digestion

... Vector: DNA (or RNA) used to artificially carry foreign material into another cell. Plasmid: Circular piece of double stranded DNA used as a vector for bacterial cells. A plasmid is a vector but not all vectors are plasmids. Multiple Cloning Site (MCS): A region of the plasmid containing many restri ...
CB - Human Genome WS 2pp
CB - Human Genome WS 2pp

... Matching In the space provided, write the letter of the definition that best matches each term. c ...
adjuvants - Ac-11
adjuvants - Ac-11

... dimers are a common cause of mutations in DNA. Another type of DNA mutation is caused by stochastic changes in DNA during replication. These errors are constantly corrected by DNA repair proteins, which are referred to as the DNA mismatch repair system of the cell. This machinery detects and correct ...
Advanced Environmental Biotechnology II
Advanced Environmental Biotechnology II

... substances such as humic acids that interfere with later molecular analysis. Also, a large proportion of directly extracted DNA might come from non-bacterial sources or from free DNA. ...
Oct29 - Staff Web Pages
Oct29 - Staff Web Pages

... Sequence of nucleotides in DNA of a gene is transcribed into mRNA. Ribosomes move along mRNA (5' to 3' direction) and read nucleotide sequence of mRNA one codon at a time. ...
recombinant dna technology and genetic engineering
recombinant dna technology and genetic engineering

... sources with the same restriction endonuclease (BamHI in this case). BamHI cuts the same site on both molecules 5' GGATCC 3' 3' CCTAGG 5'. The ends of the cut have an overhanging piece of single-stranded DNA. These are called "sticky ends" because they are able to base pair with any DNA molecule con ...
File - Science with Mrs. Levin
File - Science with Mrs. Levin

... nitrogen bases are in an ___________ along a gene and form the genetic code  that determines what type of _____________ will be produced; the order of the  three­base code unit determines a specific ____________  ___________ and  amino acids are put together to form a protein  ...
Ch11_Lecture no writing
Ch11_Lecture no writing

... • A complex of proteins, most notably DNA polymerases, is involved • New DNA is polymerized in one direction only • Since the 2 strands are antiparallel, 1 strand is made continuously and the other is made in Okazaki fragments that are eventually joined ...
Interpretation of Complex Forensic DNA Mixtures
Interpretation of Complex Forensic DNA Mixtures

THINK ABOUT IT - WordPress.com
THINK ABOUT IT - WordPress.com

G T A C A T C T T A A C G C A T A T
G T A C A T C T T A A C G C A T A T

... “unzipped” and the mRNA strand copies a strand of DNA. Once it does this, mRNA leaves the nucleus and goes into the cytoplasm. mRNA will then attach itself to a ribosome. The strand of mRNA is then read in order to make protein. They are read 3 bases at a time. These bases are called codons. tRNA is ...
No Slide Title
No Slide Title

... strands, and then bundled up even more. In a cell that has a nucleus, the strands of DNA and proteins are bundled into chromosomes. • A gene consists of a string of nucleotides that give the cell information about how to make a specific trait. ...
Unity of Life
Unity of Life

... proteins serve as enzymes and catalysts the same 20 amino acids are always used, and only left-handed ones a universal genetic code DNA triplets coding for same amino acid the use of proteins and lipids to make membranes the use of the ATP-ADP cycle for chemical energy. ...
Chapter 4: Cytogenetics
Chapter 4: Cytogenetics

Bio_Ch7 - Faustina Academy
Bio_Ch7 - Faustina Academy

... -spindle begins to disintergrate -plasma membrane begins to constrict along equatorial plane -two cells begin to form -nuclear membrane forms around each chromosome - chromosomes uncoil from their condensed form back into chromatin ...
Protein Synthesis Skit
Protein Synthesis Skit

... mRNA - Fly into Ribosome, carrying amino acid - Match up anticodon, with next codon on mRNA - Fly into Ribosome, carrying amino acid - Match up anticodon, with next codon on mRNA - Fly into Ribosome, carrying amino acid - Match up anticodon, with stop codon on mRNA - Fly into Ribosome, carrying amin ...
Section 13-2
Section 13-2

... 1. Think of two very different breeds of dogs that are familiar to you. On a sheet of paper, construct a table that has the following three heads: the name of each of the two dog breeds, and “Cross-Breed.” 2. The rows of the table should be labeled with characteristics found in both breeds of dogs. ...
Mutation Study Guide
Mutation Study Guide

... 7. In a frameshift mutation, what is the “frame” that is being shifted? This mutation involves the insertion or deletion of a nucleotide in a DNA sequence, which shifts the entire sequence by one or more nucleotides – throwing off the reading frame. 8. How might a point mutation in a gene affect the ...
Decoding mRNA
Decoding mRNA

... DNA. This copy is called 5. _________________________ and can leave the cell’s nucleus. It travels to the 6.___________________ in the cytoplasm of the cell where DNA’s message can be decoded into a sequence of amino acids. The steps of creating an mRNA transcript need to be put in order. 7. Place t ...
PowerPoint Lecture Chapter 9
PowerPoint Lecture Chapter 9

... A. Entire organisms can be cloned 1. clone- genetically identical copy of gene or of an organism 2. Some simple animals can essentially ...
DNA - Belle Vernon Area School District
DNA - Belle Vernon Area School District

... • DNA contains the information needed for replication ...
DNA Structure and Function
DNA Structure and Function

Express Letter A New Self-Fabrication of Large
Express Letter A New Self-Fabrication of Large

... and as an antibacterial film.5) When it has high conductivity, DNA can be utilized as an electric circuit in itself. Even with low conductivity, DNA can be utilized as an ultra-minute molecular circuit after modification using other molecules.6) DNA film and networks therefore have the potential to ...
Genetics Study Guide
Genetics Study Guide

... the denaturation. Therefore, strands with high content C G bases, bases which are held together by three H bonds, will require higher heat to denature than those strands having high content of A T bases , bases which are held together by only 2 H bonds.. The temperature at which half of the hydrogen ...
MUTATIONS
MUTATIONS

... chunk from one side of the DNA containing the mistake. Then new enzymes, using the base pairing code, make a new side from the opposite strand of DNA.  The new strand is put into place by another enzyme system. ...
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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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