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9-Molecular bio
9-Molecular bio

... The phosphate group of one nucleotide bonds covalently with the sugar molecule of the next nucleotide, and so on, forming a long polymer of nucleotide monomers. The sugar–phosphate groups line up in a “backbone” for each single strand of DNA, and the nucleotide bases stick out from this backbone. Th ...
The double helix: a tale of two puckers
The double helix: a tale of two puckers

File
File

... Chains are directional according to the attachment between sugars and phosphate group They are antiparallel which is essential for gene coding and replication DNA molecule has 2 separate chains of nucleotides hold together by base pairing / DNA normally twist into a helix (coil) / forms a double hel ...
Slide 1
Slide 1

... •Any two unrelated individuals differ by one base pair every 1,000 or so, referred to as SNPs. •Many SNPs have no effect on cell function and therefore can be used as molecular markers. ...
ch11dna - cpolumbo
ch11dna - cpolumbo

... PCR—Polymerase Chain Reaction Procedure  Heat the DNA strands, causing the strands to separate (unzip).  Cool the mixture and add a primer, a short sequence of base pairs that will add to its complementary sequence on the DNA strand.  Finally, add a DNA polymerase and a mixture of free nucleotid ...
Strawberry DNA Extraction Lab
Strawberry DNA Extraction Lab

3.8 DNA
3.8 DNA

... • Replication is the duplication of DNA that occurs in the S Phase of Cell Cycle • The two strands of DNA separate and each acts as a template for the formation of a new strand. ...
Supplemental Data Methods
Supplemental Data Methods

DNA
DNA

... dogma” has nothing of a dogma. On the contrary, what he proposed fully deserves to be qualified of “scientific theory”.  This formulation is admirably clear and seems to be as valid today as on the first day when it had been formulated. Even the prion makes no exception to this dogma, since Crick d ...
mcb122 tutorial kit - Covenant University
mcb122 tutorial kit - Covenant University

... organisms. 10. Nucleic acid hybridization tests the ability of DNA from different sources to base pair with each other. The more similar the DNA sequence is, the greater the amount of pairing, or hybridization. The more hybridization that occurs, the greater the degree of relationship. This techniqu ...
lecture 12, part 2, dna technology, 050509c
lecture 12, part 2, dna technology, 050509c

Meaning and Molecular Data - Circle
Meaning and Molecular Data - Circle

... The bases in DNA will only pair in very specific ways, G with C and A with T In short DNA sequences, imprecise base pairing will not be tolerated Long sequences can tolerate some mispairing only if -G of the majority of bases in a sequence exceeds the energy required to keep mispaired bases togethe ...
Real Time of PCR - KSU Faculty Member websites
Real Time of PCR - KSU Faculty Member websites

... withstand extremely high temperatures), free nucleotides (dNTPs for DNA, NTPs for RNA), and buffer. This movie shows PCR in action. The temperature is then alternated between hot and cold to denature and reanneal the DNA, with the polymerase adding new complementary strands each time. In addition to ...
Characteristics of life
Characteristics of life

... – Amount of peptidoglycan defines Gram stain. Large amount Gram-positive; low amount Gram-negative ...
Changes in Prokaryotic Transcription: Phage Lambda and Others
Changes in Prokaryotic Transcription: Phage Lambda and Others

... After a host cell is infected the phage SPO1, a series of alterations occur that change the transcription specificity of the host RNA polymerase. There are three stages to viral gene expression that can be observed. Each of these uses a different sigma factor in conjunction with the host core enzyme ...
mutation
mutation

chapter12a
chapter12a

幻灯片 1
幻灯片 1

... percentage of the solid support-bound 5'-OH groups (0.1 to 1%) remains unreacted and needs to be permanently blocked from further chain elongation to prevent the formation of oligonucleotides with an internal base deletion commonly referred to as (n-1) shortmers. This is done by acetylation (乙酰化作用)o ...
drug-design
drug-design

Nucleic Acids - OpenStax CNX
Nucleic Acids - OpenStax CNX

... two strands running in opposite directions, connected by hydrogen bonds, and complementary to each other. RNA is single-stranded and is made of a pentose sugar (ribose), a nitrogenous base, and a phosphate group. RNA is involved in protein synthesis and its regulation. Messenger RNA (mRNA) is copied ...
molecular core facility - College of William and Mary
molecular core facility - College of William and Mary

No Slide Title
No Slide Title

... These genes can even come from a very different type of organism. This shows the universal genetic code for life on earth. Ex: firefly  tobacco plant Human genes bacteria to make insulin. Bovine Somatotropic Hormone (BST, also known as BGH) has been successfully introduced and its use approved. Th ...
Genes and Chromosomes
Genes and Chromosomes

... Restriction enzymes are DNA-cutting enzymes found in bacteria Because they cut within the DNA molecule, they are often called restriction endonucleases A restriction enzyme recognizes and cuts DNA only at a particular sequence of nucleotides. For example, the bacterium Hemophilus aegypticus produces ...
9/30 - Utexas
9/30 - Utexas

... •Gene expression can be controlled at many points between DNA and making the final proteins. •Changes in the various steps of gene expression control when and how much of a product are produced. ...
13.2 abbreviated Interactive Text
13.2 abbreviated Interactive Text

... In the previous section you learned that selective breeding increases the frequency of desired traits, or alleles, in a population. You also learned that selective breeding techniques such as inbreeding and creating hybrids take time. In many cases the offspring have to mature before the traits beco ...
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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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