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Rozema Biology—Spring 2010 Protein Synthesis Skit TRANSCRIPTION Role Nucleus Job THE PLACE WHERE TRANSCRIPTION OCCURS! - Unwind the twisted DNA Costume - Lab Table Area - Enclosed by “Caution” Tape - “Unwind” Name Tag Unzip Enzyme - Separate the 2 strands of DNA - Use scissors to cut apart nitrogenous bases - “Unzip” Name Tag Match-Up Enzyme - Match up complimentary RNA bases to DNA - “Match-Up” Name Tag Bonding Enzyme - Tape RNA nucleotides together - Tape DNA back together - “Bond Together” Name Tag mRNA - Carry the genetic code from DNA out of the nucleus - Carry the mRNA strand out of the Nucleus Unwind Enzyme Rozema Biology—Spring 2010 TRANSLATION Role tRNA #1 tRNA #2 tRNA #3 tRNA #4 mRNA Ribosome & rRNA Polypeptide MATERIALS NEEDED: (1) Caution Tape (4) Name Tags Scotch Tape Scissors Colored Paper for DNA Colored Paper for mRNA (16) Party Hats (16) Balloons (4) Smaller Foam Boards (2) Gigantic Foam Boards (1) Plastic Ribbon (1) Cloth Ribbon Packaging Tape (1) Sign Nucleus (1) Sign Cytoplasm Job - Match up anticodon, with start codon on mRNA - Fly into Ribosome, carrying amino acid - Match up anticodon, with next codon on mRNA - Fly into Ribosome, carrying amino acid - Match up anticodon, with next codon on mRNA - Fly into Ribosome, carrying amino acid - Match up anticodon, with stop codon on mRNA - Fly into Ribosome, carrying amino acid - carry the genetic code from DNA into the cytoplasm - move through ribosome - meet with complimentary tRNA molecules to transfer amino acids - organize tRNA and mRNA to make polypeptide END PRODUCT!! Costume - Part Hat w/Balloon (Amino Acid) - tRNA Name Board - Party Hat w/Balloon (Amino Acid) - tRNA Name Board - Party Hat w/Balloon (Amino Acid) - tRNA Name Board - Party Hat w/Balloon (Amino Acid) - tRNA Name Board - Carry the mRNA strand through the Ribosome - Giant Poster Board shaped like hamburger buns - Balloons Connected Together