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DNA Databases - Glasgow Science Centre
DNA Databases - Glasgow Science Centre

introduction
introduction

... Genome Express). Single Strand DNA library According to the GS pyrosequencing protocol, gDNA must first be transformed into a library of single-strand template DNA fragments (sstDNA) flanked with amplification and sequencing primer sequences. These sstDNA libraries were prepared using the GS Library ...
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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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