Chapter 18 Practice Multiple Choice
... c. methylation of the DNA is maintained because methylation enzymes act at DNA sites where one strand is already methylated and thus correctly methylates daughter strands after replication. d. methylation of the DNA is maintained because DNA polymerase directly incorporates methylated nucleotides in ...
... c. methylation of the DNA is maintained because methylation enzymes act at DNA sites where one strand is already methylated and thus correctly methylates daughter strands after replication. d. methylation of the DNA is maintained because DNA polymerase directly incorporates methylated nucleotides in ...
High Frequency of Recombination (Hfr)
... • Untreated culture Do a serial dilution of the untreated wildtype E. coli culture: Fill 7 tubes with 4.5 ml of sterile saline. Transfer 0.5 ml of the undiluted culture to one of the tubes. This is a 10-1 dilution. Next make serial dilutions of 10-2, 10-3, 10-4, 10-5, 10-6 and 10-7. Always change pi ...
... • Untreated culture Do a serial dilution of the untreated wildtype E. coli culture: Fill 7 tubes with 4.5 ml of sterile saline. Transfer 0.5 ml of the undiluted culture to one of the tubes. This is a 10-1 dilution. Next make serial dilutions of 10-2, 10-3, 10-4, 10-5, 10-6 and 10-7. Always change pi ...
Why are we all so different? DNA Extraction
... DNA instructions are divided into segments called genes. Each gene provides the information for making a protein, which carries out a specific function in the cell. A molecule of DNA (DeoxyriboNucleic Acid) is composed of two backbones and four types of chemical bases. The backbone is formed by a ch ...
... DNA instructions are divided into segments called genes. Each gene provides the information for making a protein, which carries out a specific function in the cell. A molecule of DNA (DeoxyriboNucleic Acid) is composed of two backbones and four types of chemical bases. The backbone is formed by a ch ...
Genetics Course Outcome Summary Course Information
... c. Identify enzymes which play a role in DNA replication and recombination d. Explain analytical techniques which have been useful to the investigation of DNA and RNA Describe recombinant DNA technology. Learning Objectives a. Describe the basic steps involved in recombinant DNA and the uses of this ...
... c. Identify enzymes which play a role in DNA replication and recombination d. Explain analytical techniques which have been useful to the investigation of DNA and RNA Describe recombinant DNA technology. Learning Objectives a. Describe the basic steps involved in recombinant DNA and the uses of this ...
Teacher Notes PDF - TI Education
... restriction site regardless of the organism (humans included). In the laboratory, molecular biologists can use restriction enzymes to cut up DNA from two different organisms and then splice these pieces of DNA to one another, thus creating recombinant DNA. Once new DNA is placed into a host organism ...
... restriction site regardless of the organism (humans included). In the laboratory, molecular biologists can use restriction enzymes to cut up DNA from two different organisms and then splice these pieces of DNA to one another, thus creating recombinant DNA. Once new DNA is placed into a host organism ...
DNA* Cow vs. Banana
... such as making a specific protein. But DNA can mutate. Even one simple mutation in the DNA can have extreme effects such as sickle cell disease. DNA is even found in some viruses. A strand of DNA contains chemicals called nucleotides. A DNA molecule is made up of 2 polynucleotide chains arranged on ...
... such as making a specific protein. But DNA can mutate. Even one simple mutation in the DNA can have extreme effects such as sickle cell disease. DNA is even found in some viruses. A strand of DNA contains chemicals called nucleotides. A DNA molecule is made up of 2 polynucleotide chains arranged on ...
Overview of the Recombinant DNA technology- the plasmid vector pUC19
... needs to be cut up in a precise and repeatable way by using enzymes. Therefore, the foreign gene needs to be cut out of the pBK-CMV with the restriction endonucleases EcoR1 and Xbal, same as the pUC19. Restriction endonucleases recognize certain DNA sequences which are polindromic, usually 4-6 base- ...
... needs to be cut up in a precise and repeatable way by using enzymes. Therefore, the foreign gene needs to be cut out of the pBK-CMV with the restriction endonucleases EcoR1 and Xbal, same as the pUC19. Restriction endonucleases recognize certain DNA sequences which are polindromic, usually 4-6 base- ...
Genetic engineering
... - different enzymes cut DNA at specific base sequences known as a recognition site. For example i) One restriction enzyme will always cut DNA at the base sequence: GAATTC. ii) Another restriction enzyme only cuts at the sequence: GATC. - If DNA from two different organisms is cut with the same restr ...
... - different enzymes cut DNA at specific base sequences known as a recognition site. For example i) One restriction enzyme will always cut DNA at the base sequence: GAATTC. ii) Another restriction enzyme only cuts at the sequence: GATC. - If DNA from two different organisms is cut with the same restr ...
Chromomere - aqinfo.com
... If telomeres are damaged/removed – end are highly unstable and fuse with broken ends of other chromosomes – resulting in translocations or ring chromosomes Structural identity and individuality of chromosome is maintained due to telomeres ...
... If telomeres are damaged/removed – end are highly unstable and fuse with broken ends of other chromosomes – resulting in translocations or ring chromosomes Structural identity and individuality of chromosome is maintained due to telomeres ...
Viruses
... 8. Protein degradation • 3-D stage of protein changes shape as protein ages, marked by ubiquitin for destruction ...
... 8. Protein degradation • 3-D stage of protein changes shape as protein ages, marked by ubiquitin for destruction ...
No Slide Title
... information from the DNA in the nucleus out to the ribosomes; 2) Ribosomal RNA (rRNA): clamp on to the mRNA and use its information to assemble amino acids into a protein; 3) Transfer RNA (tRNA): the “supplier”; transports amino acids to the ribosome ...
... information from the DNA in the nucleus out to the ribosomes; 2) Ribosomal RNA (rRNA): clamp on to the mRNA and use its information to assemble amino acids into a protein; 3) Transfer RNA (tRNA): the “supplier”; transports amino acids to the ribosome ...
Prentice Hall Biology
... 4. How could you use the original paper to draw exact copies of the line without tracing it? Possible answer: Cut along the line and use it as a template to draw the line on another sheet of paper. 5. Why is it important that the copies of DNA that are given to new daughter cells be exact copies of ...
... 4. How could you use the original paper to draw exact copies of the line without tracing it? Possible answer: Cut along the line and use it as a template to draw the line on another sheet of paper. 5. Why is it important that the copies of DNA that are given to new daughter cells be exact copies of ...
protein synthesis lab
... To define different types of mutations. To understand the three types of point mutations; silent, missense, and nonsense. To understand how an addition or deletion of a nucleotide causes a frameshift mutation. To understand the four types of chromosomal mutations; deletion, duplication, inversion, t ...
... To define different types of mutations. To understand the three types of point mutations; silent, missense, and nonsense. To understand how an addition or deletion of a nucleotide causes a frameshift mutation. To understand the four types of chromosomal mutations; deletion, duplication, inversion, t ...
Final Exam Study Guide 2015
... ◦ Be able to perform Punnett squares for standard inheritance, codominance, incomplete dominance, sexlinked inheritance, and multiple alleles (blood type) and predict genotype and phenotype ratios ◦ Understand and be able to define each form of inheritance listed above Genetic Disorders ◦ Know how a ...
... ◦ Be able to perform Punnett squares for standard inheritance, codominance, incomplete dominance, sexlinked inheritance, and multiple alleles (blood type) and predict genotype and phenotype ratios ◦ Understand and be able to define each form of inheritance listed above Genetic Disorders ◦ Know how a ...
Biology - Asbury Park School District
... 10. Once to the open space, have the students examine their current positions. Are they in a long line? Taking up a lot of space? Without the students moving, ask them how hard it would be for them to fit in a tiny space and then stretch out the helix so it can be read. Ask for ideas, there cannot b ...
... 10. Once to the open space, have the students examine their current positions. Are they in a long line? Taking up a lot of space? Without the students moving, ask them how hard it would be for them to fit in a tiny space and then stretch out the helix so it can be read. Ask for ideas, there cannot b ...
Asbury Park School District
... in the process of cellular division, which passes traits from one generation to the next. Students determine why individuals of the same species vary in how they look, function, and behave. Students develop conceptual models of the role of DNA in the unity of life on Earth and use statistical models ...
... in the process of cellular division, which passes traits from one generation to the next. Students determine why individuals of the same species vary in how they look, function, and behave. Students develop conceptual models of the role of DNA in the unity of life on Earth and use statistical models ...
genomic library
... • Restriction enzymes cut DNA into specific fragments • Restriction enzymes recognize specific base sequences in double-stranded DNA and cleave both strands of the duplex at specific places • Characteristics of restriction enzymes: 1. Cut DNA sequence-specifically 2. Bacterial enzymes; hundreds are ...
... • Restriction enzymes cut DNA into specific fragments • Restriction enzymes recognize specific base sequences in double-stranded DNA and cleave both strands of the duplex at specific places • Characteristics of restriction enzymes: 1. Cut DNA sequence-specifically 2. Bacterial enzymes; hundreds are ...
DNA Isolation and Genetic Transformation page 66
... DNA Isolation and Genetic Transformation bly protects the onion from being eaten by some organisms). We will homogenize the onion in an "extraction solution" that contains the laundry liquid "Woolite" and NaCl. Woolite contains detergents that will dissolve the cell membranes, and also proteolytic ...
... DNA Isolation and Genetic Transformation bly protects the onion from being eaten by some organisms). We will homogenize the onion in an "extraction solution" that contains the laundry liquid "Woolite" and NaCl. Woolite contains detergents that will dissolve the cell membranes, and also proteolytic ...
CHAPTER 6
... Non-Watson-Crick G:U base pairs represent additional regular base pairing in RNA, which enriched the capacity for self-complementarity. ...
... Non-Watson-Crick G:U base pairs represent additional regular base pairing in RNA, which enriched the capacity for self-complementarity. ...
12.3 DNA, RNA, and Protein
... – Nucleotides pair with one strand of the DNA. – RNA polymerase bonds the nucleotides together. – The DNA helix winds again as the gene is transcribed. DNA ...
... – Nucleotides pair with one strand of the DNA. – RNA polymerase bonds the nucleotides together. – The DNA helix winds again as the gene is transcribed. DNA ...
Amplification of DNA Sequences
... enzyme will generate new DNA strands by incorporating individual nucleotide bases that are provided in the reaction mixture (Fig 1). After synthesis of the new DNA strands, the reaction mixture again is heated to 96°C to denature the newly generated DNA pieces from the original DNA strands. These ne ...
... enzyme will generate new DNA strands by incorporating individual nucleotide bases that are provided in the reaction mixture (Fig 1). After synthesis of the new DNA strands, the reaction mixture again is heated to 96°C to denature the newly generated DNA pieces from the original DNA strands. These ne ...
Chapter 13: Genetic Technology
... Smaller DNA fragments move faster and further How do you end up with different size fragments that are unique to each individual? Tandem Repeat – region of a chromosome that contains multiple copies of a DNA sequence The origin and significance of tandem repeats is a mystery For forensic s ...
... Smaller DNA fragments move faster and further How do you end up with different size fragments that are unique to each individual? Tandem Repeat – region of a chromosome that contains multiple copies of a DNA sequence The origin and significance of tandem repeats is a mystery For forensic s ...
Replisome
The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.