SOP 105: Procedures for DNA gel electrophoresis.
... Agarose gels can be used for separation and extraction of DNA fragments, for example, a specific DNA fragment from a PCR or restriction digestion reaction. Ensure that the percentage of agarose used for the gel allows good separation of DNA fragments for easy excision. Run agarose gels for DNA extra ...
... Agarose gels can be used for separation and extraction of DNA fragments, for example, a specific DNA fragment from a PCR or restriction digestion reaction. Ensure that the percentage of agarose used for the gel allows good separation of DNA fragments for easy excision. Run agarose gels for DNA extra ...
Teacher Guide - the BIOTECH Project
... This teacher guide is provided to give sample answers to questions. Most of the questions are open-ended, so students may have correct answers that aren't included in this guide. Finally, although the experiment is set up to yield one correct answer, there are variations in data between students. As ...
... This teacher guide is provided to give sample answers to questions. Most of the questions are open-ended, so students may have correct answers that aren't included in this guide. Finally, although the experiment is set up to yield one correct answer, there are variations in data between students. As ...
Unit 2
... • Several replication forks form on the DNA strand and the process continues until all of the DNA has been replicated. ▫ If only one was formed it would take too long to replicate DNA (53 days for humans!!) • When replication is finished, there are two DNA molecules. Each has one old strand and one ...
... • Several replication forks form on the DNA strand and the process continues until all of the DNA has been replicated. ▫ If only one was formed it would take too long to replicate DNA (53 days for humans!!) • When replication is finished, there are two DNA molecules. Each has one old strand and one ...
power point
... • Accused of drugging and sexually assaulting patients, DNA profiles from semen samples from the assaulted women do not match Dr. Schneeberger • Blood was drawn on 3 occasions in 1992, 1993 and 1996, but never came back as a match • Finally police obtain blood from a finger prick, swabbed the inside ...
... • Accused of drugging and sexually assaulting patients, DNA profiles from semen samples from the assaulted women do not match Dr. Schneeberger • Blood was drawn on 3 occasions in 1992, 1993 and 1996, but never came back as a match • Finally police obtain blood from a finger prick, swabbed the inside ...
Lecture 4
... - Two polynucleotide strands wrap around each other to form a DNA double helix - The two strands are associated because particular bases always hydrogen bond to one another A pairs with T, and C pairs with G, producing base pairs - RNA is usually a single polynucleotide strand ...
... - Two polynucleotide strands wrap around each other to form a DNA double helix - The two strands are associated because particular bases always hydrogen bond to one another A pairs with T, and C pairs with G, producing base pairs - RNA is usually a single polynucleotide strand ...
Biology 40S – Final Exam Review (2013
... hijack their ribosomes and enzymes to recreate their proteins and DNA/RNA for them. Otherwise, in the environment, they are incapable of performing any of the basic functions of life – they do not consume energy in any form, and they have no organelles or cellular structures to perform tasks essenti ...
... hijack their ribosomes and enzymes to recreate their proteins and DNA/RNA for them. Otherwise, in the environment, they are incapable of performing any of the basic functions of life – they do not consume energy in any form, and they have no organelles or cellular structures to perform tasks essenti ...
7. According to Dr. Malcolm (guy in black leather jacket), “Dinosaurs
... from the mother, and one from the father. The chromosome starts as half of the familiar X. As the cell grows, it replicates the DNA to make the other half of the X, which is identical. When the cell divides, each daughter cell receives half of each chromosome (called a chromatid). The two copies of ...
... from the mother, and one from the father. The chromosome starts as half of the familiar X. As the cell grows, it replicates the DNA to make the other half of the X, which is identical. When the cell divides, each daughter cell receives half of each chromosome (called a chromatid). The two copies of ...
Chapter 20.
... Restriction sites for splicing in gene of interest Selectable marker Plasmid has both “added” gene & antibiotic resistance gene If bacteria don’t pick up plasmid then die on antibiotic plates If bacteria pick up plasmid then survive on antibiotic plates selecting for successful AP Biology ...
... Restriction sites for splicing in gene of interest Selectable marker Plasmid has both “added” gene & antibiotic resistance gene If bacteria don’t pick up plasmid then die on antibiotic plates If bacteria pick up plasmid then survive on antibiotic plates selecting for successful AP Biology ...
The Medicinal Chemistry of Antibiotics
... acids prevail almost universally (in proteins and enzymes in all organisms), but there are some notable exceptions, like the peptide components of bacterial cell walls. Bacteria contain racemase enzymes that can convert L-amino acids into D-amino acids, a biochemical feature that is absent in higher ...
... acids prevail almost universally (in proteins and enzymes in all organisms), but there are some notable exceptions, like the peptide components of bacterial cell walls. Bacteria contain racemase enzymes that can convert L-amino acids into D-amino acids, a biochemical feature that is absent in higher ...
Assembly of microarrays for genome-wide measurement of
... well, into which we added 50 l of slide blocking solution containing 500 g salmon sperm DNA in 50% formamide, 2 x SSC, 10% dextran sulfate and 4% SDS, pH 7. We created an airtight hybridization chamber by placing a silicone gasket (PGC Scientific) around the array and rubber cement ring, placing a ...
... well, into which we added 50 l of slide blocking solution containing 500 g salmon sperm DNA in 50% formamide, 2 x SSC, 10% dextran sulfate and 4% SDS, pH 7. We created an airtight hybridization chamber by placing a silicone gasket (PGC Scientific) around the array and rubber cement ring, placing a ...
Slide 1
... • Copies the DNA code to mRNA – DNA unwinds – Only 1 strand is transcribed – Complimentary base pairing of nucleotides C-G G-C T-A A-U – This pairing forms the mRNA – RNA polymerase helps form the new mRNA • initiation signal - “start here” • termination sequence - “stop synthesis” ...
... • Copies the DNA code to mRNA – DNA unwinds – Only 1 strand is transcribed – Complimentary base pairing of nucleotides C-G G-C T-A A-U – This pairing forms the mRNA – RNA polymerase helps form the new mRNA • initiation signal - “start here” • termination sequence - “stop synthesis” ...
Chapter 17: Gene Expression Gene Expression DNA houses all
... o Single-stranded & one gene RNA Polymerase o Opens DNA o Builds RNA strand on 3’ 5’ template (like leading strand) o RNA Pol only adds to 3’ end, so RNA built in 5’ 3’ direction Base pairs = G-C & A-U The Genetic Code o Must code for 20 amino acids o Codon If each base a ‘letter’, how l ...
... o Single-stranded & one gene RNA Polymerase o Opens DNA o Builds RNA strand on 3’ 5’ template (like leading strand) o RNA Pol only adds to 3’ end, so RNA built in 5’ 3’ direction Base pairs = G-C & A-U The Genetic Code o Must code for 20 amino acids o Codon If each base a ‘letter’, how l ...
Section F
... regions between two DNA molecules, it is also known as postreplication repair. The integrity of DNA containing un-repaired lesions can be fixed during replication by homologous recombination. • Mechanism: 1. In eukaryotes, this commonly occurs during meiosis, when the homologous duplicated chromosom ...
... regions between two DNA molecules, it is also known as postreplication repair. The integrity of DNA containing un-repaired lesions can be fixed during replication by homologous recombination. • Mechanism: 1. In eukaryotes, this commonly occurs during meiosis, when the homologous duplicated chromosom ...
Models for homologous recombination
... meiotic recombination Many protein function together to promote meiotic recombination ...
... meiotic recombination Many protein function together to promote meiotic recombination ...
2. If 20% of the DNA in a guinea pig cell is adenine, what
... 5. Scientists struggled to understand how four bases could code for 20 different amino acids. If one base coded for one amino acid, the cell could produce only four different kinds of amino acids (41). If two bases coded for each amino acid, there would be four possible choices (of nucleotides) for ...
... 5. Scientists struggled to understand how four bases could code for 20 different amino acids. If one base coded for one amino acid, the cell could produce only four different kinds of amino acids (41). If two bases coded for each amino acid, there would be four possible choices (of nucleotides) for ...
Chapter 16. - RMC Science Home
... developed double helix model of DNA other leading scientists working on question: ...
... developed double helix model of DNA other leading scientists working on question: ...
Lecture 2 (1/25/10) "The Language of Life"
... systems in their living environment is much too complex. Eventually, it might not be so, but for now it is. One system which is amenable to quantitative reasoning, is the study of protein folding, a field Hans Frauenfelder , former Professor of Physics and founder of biological physics: became famou ...
... systems in their living environment is much too complex. Eventually, it might not be so, but for now it is. One system which is amenable to quantitative reasoning, is the study of protein folding, a field Hans Frauenfelder , former Professor of Physics and founder of biological physics: became famou ...
Jeopardy - Montville.net
... This metabolic pathway requires energy to build up larger molecules. ...
... This metabolic pathway requires energy to build up larger molecules. ...
Introduction - OpenWetWare
... ligases can join the ends of two different DNA fragments, or join the two ends of a single fragment. There are two types of ligation, called sticky-end ligation and blunt-end ligation, whose names refer to the presence or absence of short overhangs on the ends of the DNA being ligated. Blunt-end lig ...
... ligases can join the ends of two different DNA fragments, or join the two ends of a single fragment. There are two types of ligation, called sticky-end ligation and blunt-end ligation, whose names refer to the presence or absence of short overhangs on the ends of the DNA being ligated. Blunt-end lig ...
Deletion of DNA sequences of using a polymerase chain
... plasmid sequence in the 5' end of the region to be deleted and contains in the 5' end the same sequence as the primer forward to be cut by a restriction enzyme, preceded by one or two bases to keep the reading frame and to avoid introducing one stop codon. It may also be important to consider the pr ...
... plasmid sequence in the 5' end of the region to be deleted and contains in the 5' end the same sequence as the primer forward to be cut by a restriction enzyme, preceded by one or two bases to keep the reading frame and to avoid introducing one stop codon. It may also be important to consider the pr ...
BCH-201:Nucleotides and Nucleic acids
... Messenger RNA comes in a wide range of sizes reflecting the size of the polypeptide it encodes. Most cells produce small amounts of thousands of different mRNA molecules, each to be translated into a peptide needed by the cell. Many mRNAs are common to most cells, encoding "housekeeping" proteins ne ...
... Messenger RNA comes in a wide range of sizes reflecting the size of the polypeptide it encodes. Most cells produce small amounts of thousands of different mRNA molecules, each to be translated into a peptide needed by the cell. Many mRNAs are common to most cells, encoding "housekeeping" proteins ne ...
lecture1
... Messenger RNA comes in a wide range of sizes reflecting the size of the polypeptide it encodes. Most cells produce small amounts of thousands of different mRNA molecules, each to be translated into a peptide needed by the cell. Many mRNAs are common to most cells, encoding "housekeeping" proteins ne ...
... Messenger RNA comes in a wide range of sizes reflecting the size of the polypeptide it encodes. Most cells produce small amounts of thousands of different mRNA molecules, each to be translated into a peptide needed by the cell. Many mRNAs are common to most cells, encoding "housekeeping" proteins ne ...
Protists
... PROTISTS- Chapter 28 “More about what they are not… than what they are” CHARACTERISTICS EUKARYOTES that AREN’T animals, plants, or fungi Primarily unicellular (Paramecium, Euglena); Some colonial (Volvox); some multicellular (Seaweed) Cilia or flagella at some time in life cycle Kingdom is POLYPHYLE ...
... PROTISTS- Chapter 28 “More about what they are not… than what they are” CHARACTERISTICS EUKARYOTES that AREN’T animals, plants, or fungi Primarily unicellular (Paramecium, Euglena); Some colonial (Volvox); some multicellular (Seaweed) Cilia or flagella at some time in life cycle Kingdom is POLYPHYLE ...
Multiple Choice - 28 points total In each of the questions
... As you know, the Meselson-Stahl experiment provided clear evidence that DNA replication is semi-conservative. The experiment involved the separation of "heavy" DNA (labeled with 15N) from "light" DNA (containing 14N) by centrifugation in a tube containing a cesium chloride (CsCl) density gradient. T ...
... As you know, the Meselson-Stahl experiment provided clear evidence that DNA replication is semi-conservative. The experiment involved the separation of "heavy" DNA (labeled with 15N) from "light" DNA (containing 14N) by centrifugation in a tube containing a cesium chloride (CsCl) density gradient. T ...
Replisome
The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.