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RNA Polymerase
RNA Polymerase

Ch. 12.3 and 12.4 ppt
Ch. 12.3 and 12.4 ppt

The evolution of life science methodologies: From single gene
The evolution of life science methodologies: From single gene

... based detection of fluorescent labels incorporated with each  extension can be used to acquire sequencing data on all  features in parallel. Successive iterations of enzymatic  interrogation and imaging are used to build up a contiguous  sequencing read for each array feature. ...
recombinant DNA. Lesson Overview
recombinant DNA. Lesson Overview

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... ____ 11. What role do ribosomes play in protein synthesis? a. It transcribes the mRNA. b. It brings amino acids to the tRNA. c. It provides the binding site for mRNA and tRNA. ...
Biotechnology - Explore Biology
Biotechnology - Explore Biology

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DNA CODES…
DNA CODES…

... has, only 1% of it actually codes for proteins. Scientists are still trying to determine what the rest of the DNA is used for. A section of DNA that codes for a protein is called a gene. DNA is found in the nucleus of a eukaryotic cell. Genes need to be TRANSCRIBED into an mRNA molecule. DNA will un ...
STRAND1 - Bulletin - Sigma
STRAND1 - Bulletin - Sigma

... G. Phosphorylation Using T4 Polynucleotide Kinase A convenient method for phosphorylating previously synthesized oligonucleotides is treatment with T4 polynucleotide kinase. Typically, 60-80% of a purified primer is phosphorylated under conditions described below. With unpurified primers, the extent ...
69 Evidence from DNA
69 Evidence from DNA

... these DNA fragments looks almost like a complicated bar code. DNA fingerprinting reveals your own unique pattern, which is almost as unique as your DNA itself. The genetic code is made up of four “letters” (A, T, C, and G), each of which stands for one of four related chemicals that are strung toget ...
Ch. 13 Genetic Engineering
Ch. 13 Genetic Engineering

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“DNA Testing for Inherited eye diseases in Border Collies”.

... DNA is found within most cells of the body and contains all the genetic information of an animal. We can sample this DNA and  test it to find out whether an animal is at risk of developing certain inherited diseases, or is at risk of passing them on to their  offspring. This is called DNA testing.   ...
Section E: Variation and Selection
Section E: Variation and Selection

... pesticides in a similar way. Gene mutations are random events that occur in all organisms. The rate at which they occur can be increased by a number of agents called mutagens. Mutagens include: ...
Previous exams
Previous exams

... Dato: 25. februar 2004 ...
Accuracy of DNA Repair During Replication in Saccharomyces
Accuracy of DNA Repair During Replication in Saccharomyces

... α errors than ε. It was also found that when both DNA polymerase δ and ε were removed, the mutation rate was significantly higher than when just one of the DNA polymerases was knocked out (Strathern et al., 2006). ...
No Slide Title
No Slide Title

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Protein Nucleic Acid Interactions
Protein Nucleic Acid Interactions

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Designer Genes - Heredity
Designer Genes - Heredity

... associated with ribosomes (rRNA + proteins). tRNAs, each carrying a specific amino acid, pair up with the mRNA codons inside the ribosomes. Base pairing (A-U, G-C) between mRNA codons and tRNA anticodons determines the order of amino acids in a protein. 2. Elongation: addition of amino acids one-by- ...
genotypes
genotypes

... consider two steps. The first is to determine if the disorder is autosomal or X-linked. • If the disorder is X-linked most of the males will have the disorder because the Y-chromosome cannot mask the affects of an affected X-chromosome. A female can have the disorder, but it would be a very low perc ...
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... The average human chromosome has DNA that contains 100 million base pairs DNA directs the production of proteins A protein is a polymer made of amino acids There are twenty known amino acids that are used to make thousands of proteins ...
Standard B-5 - Wando High School
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... It is essential for students to understand that DNA can function as the code of life for protein synthesis or the process of DNA replication, which ensures that every new cell has identical DNA.  DNA replication is carried out by a series of enzymes. The first enzyme unzips the two strands of DNA t ...
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Educational Items Section Nomenclature for the description of mutations and

... insertion of glutamine serine between lysine (K) in 2 and leucine (L) in 3 ...
PowerPoint file
PowerPoint file

... Image Source: www.ebi.ac.uk/microarray/ biology_intro.htm ...
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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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