Human RIF1 and protein phosphatase 1 stimulate DNA replication
... RIF1 targeted PP1 acts upon. The authors continue by observing minor defects in DNA replication in RIF1 overexpressing cells and ascribe this observation to reduced helicase activation, yet overexpression of the RIF1-PP1 interaction mutant does not curb this observation as their hypothesis predicts. ...
... RIF1 targeted PP1 acts upon. The authors continue by observing minor defects in DNA replication in RIF1 overexpressing cells and ascribe this observation to reduced helicase activation, yet overexpression of the RIF1-PP1 interaction mutant does not curb this observation as their hypothesis predicts. ...
The coiled-coil of the human Rad50 DNA repair protein contains
... importance is underscored by its conservation from bacteriophages to humans (1). Based on amino acid sequence similarities, Rad50 belongs to the structural maintenance of chromosomes (SMC) family of proteins. Their shared amino acid sequence predicts a very distinct structure, including a bipartite ...
... importance is underscored by its conservation from bacteriophages to humans (1). Based on amino acid sequence similarities, Rad50 belongs to the structural maintenance of chromosomes (SMC) family of proteins. Their shared amino acid sequence predicts a very distinct structure, including a bipartite ...
Protein A gene expression is regulated by DNA supercoiling which
... (Dorman, 1991; Hsieh et al., 1991). In Escherichia coli, it has been estimated that roughly 50 % of the supercoiling is constrained by proteins bound to DNA. The remaining supercoils are maintained actively at the cost of ATP hydrolysis, via topoisomerase activities (Hatfield & Benham, 2002). Thus, ...
... (Dorman, 1991; Hsieh et al., 1991). In Escherichia coli, it has been estimated that roughly 50 % of the supercoiling is constrained by proteins bound to DNA. The remaining supercoils are maintained actively at the cost of ATP hydrolysis, via topoisomerase activities (Hatfield & Benham, 2002). Thus, ...
High throughput nucleic acid sample preparation in 96 well plates
... to nucleic acid purification in 96 well format. The long drip directors guarantee the prevention of well to well contamination during the purification process, and a reduction of aerosol development. In addition, this technology leads to a reduction of foam development if detergent containing buffer ...
... to nucleic acid purification in 96 well format. The long drip directors guarantee the prevention of well to well contamination during the purification process, and a reduction of aerosol development. In addition, this technology leads to a reduction of foam development if detergent containing buffer ...
Optimization and the Robustness of BOX A1R PCR for DNA
... resolution was still low. Thus, reaction 2F was a confirmation test to ensure that the gel was not the culprit for the oddly-shaped bands and the result for reaction 2F is shown in figure 5. Again, dumb-bell shaped bands were seen in the gel. The PCR products from reaction 2G, with the same conditio ...
... resolution was still low. Thus, reaction 2F was a confirmation test to ensure that the gel was not the culprit for the oddly-shaped bands and the result for reaction 2F is shown in figure 5. Again, dumb-bell shaped bands were seen in the gel. The PCR products from reaction 2G, with the same conditio ...
Alu Background Information
... evolution by creating new mutations and gene combinations. Nobel laureate Barbara McClintock hypothesized that transposable elements provide a mechanism to rapidly reorganize the genome in response to environmental stress. Like Alu, the Ds transposable element discovered in corn by McClintock is a d ...
... evolution by creating new mutations and gene combinations. Nobel laureate Barbara McClintock hypothesized that transposable elements provide a mechanism to rapidly reorganize the genome in response to environmental stress. Like Alu, the Ds transposable element discovered in corn by McClintock is a d ...
The New Genetics - Marine Biodiversity Center
... plants were bred to each other, some of their offspring had white flowers. What’s more, the second-generation plants displayed the colors in a predictable pattern. On average, 75 percent of the second-generation plants had purple flowers and 25 percent of the plants had white flowers. Those same rat ...
... plants were bred to each other, some of their offspring had white flowers. What’s more, the second-generation plants displayed the colors in a predictable pattern. On average, 75 percent of the second-generation plants had purple flowers and 25 percent of the plants had white flowers. Those same rat ...
PDF
... The experimental approach made use of the transplantation test of Webster & Wolpert (1966) in conjunction with some inhibitors of DNA, RNA and protein synthesis, the rationale being that if these metabolic activities play important roles in the determination of the hypostome, then their inhibition w ...
... The experimental approach made use of the transplantation test of Webster & Wolpert (1966) in conjunction with some inhibitors of DNA, RNA and protein synthesis, the rationale being that if these metabolic activities play important roles in the determination of the hypostome, then their inhibition w ...
Gene Section RAD52 (RAD52 homolog (S. cerevisiae)) Atlas of Genetics and Cytogenetics
... and thus homology-directed repair (Milne and Weaver, 1993). hRAD52 also demonstrates this ability to stimulate homologous pairing by hRAD51 (Benson et al., 1998). The interaction of ScRAD52 and hRAD52 with replication protein A (RPA) is important for the binding with ssDNA by RAD52 (Hays et al., 199 ...
... and thus homology-directed repair (Milne and Weaver, 1993). hRAD52 also demonstrates this ability to stimulate homologous pairing by hRAD51 (Benson et al., 1998). The interaction of ScRAD52 and hRAD52 with replication protein A (RPA) is important for the binding with ssDNA by RAD52 (Hays et al., 199 ...
Explaining the Likelihood Ratio in DNA Mixture Interpretation
... straightforward mathematics. The LR here is a ratio of two likelihoods: the probability of the data given the identification hypothesis, divided by the probability of the data given the alternative hypothesis. In plain language, we might state the LR as "the probability of observing the evidence ass ...
... straightforward mathematics. The LR here is a ratio of two likelihoods: the probability of the data given the identification hypothesis, divided by the probability of the data given the alternative hypothesis. In plain language, we might state the LR as "the probability of observing the evidence ass ...
Explaining the Likelihood Ratio in DNA Mixture
... straightforward mathematics. The LR here is a ratio of two likelihoods: the probability of the data given the identification hypothesis, divided by the probability of the data given the alternative hypothesis. In plain language, we might state the LR as "the probability of observing the evidence ass ...
... straightforward mathematics. The LR here is a ratio of two likelihoods: the probability of the data given the identification hypothesis, divided by the probability of the data given the alternative hypothesis. In plain language, we might state the LR as "the probability of observing the evidence ass ...
Lampbrush Chromosomes of the Chicken
... more variable from animal to animal and even among similar sized oocytes from the same animal relative to the newt. The spread chromosome preparations were examined "live" with an inverted microscope and phase-contrast optics or, more routinely, as dry preparations before in situ hybridization or as ...
... more variable from animal to animal and even among similar sized oocytes from the same animal relative to the newt. The spread chromosome preparations were examined "live" with an inverted microscope and phase-contrast optics or, more routinely, as dry preparations before in situ hybridization or as ...
DNA damage in round spermatids of mice with a targeted disruption
... fertile and are able to sire normal offspring (Varmuza et al., 1999). Sperm samples obtained from their epididymides showed no significant difference either in sperm count, motility or morphology. However, homozygous mutant males (–/–) are sterile, and display spermatogenic arrest. Their epididymide ...
... fertile and are able to sire normal offspring (Varmuza et al., 1999). Sperm samples obtained from their epididymides showed no significant difference either in sperm count, motility or morphology. However, homozygous mutant males (–/–) are sterile, and display spermatogenic arrest. Their epididymide ...
talk
... 1978 - Schwartz and Dayhoff – Protein and RNA or DNA sequencing suggests chloroplasts are reduced forms of cyanobacteria 1993 - Hallick et al., Reith and Munholland - Entire chloroplast sequences for Euglena And Porphyra; similarity in organization of genes in operons 2002 – Sato – Elucidation of ho ...
... 1978 - Schwartz and Dayhoff – Protein and RNA or DNA sequencing suggests chloroplasts are reduced forms of cyanobacteria 1993 - Hallick et al., Reith and Munholland - Entire chloroplast sequences for Euglena And Porphyra; similarity in organization of genes in operons 2002 – Sato – Elucidation of ho ...
Physiological characterization of natural transformation in
... 1986). As more and more details of natural transformation in a number of these bacteria have been elucidated, it is becoming clear that distinct differences exist in this process between these organisms. Wellknown examples are the sequence specificity of DNA uptake in Haemophilus (Goodgal, 1982) and ...
... 1986). As more and more details of natural transformation in a number of these bacteria have been elucidated, it is becoming clear that distinct differences exist in this process between these organisms. Wellknown examples are the sequence specificity of DNA uptake in Haemophilus (Goodgal, 1982) and ...
VDJ Recombination
... nontemplate-dependent nucleotides. The nonlymphoidrestricted components identified so far are DNA-PKcs, Ku70, Ku80, XRCC4, ligase 4, and the most recently identified Artemis. All these proteins are involved in DNA double strand break repair as well as VDJ recombination. These nonlymphoid-specific co ...
... nontemplate-dependent nucleotides. The nonlymphoidrestricted components identified so far are DNA-PKcs, Ku70, Ku80, XRCC4, ligase 4, and the most recently identified Artemis. All these proteins are involved in DNA double strand break repair as well as VDJ recombination. These nonlymphoid-specific co ...
De Bruijn Graphs for DNA Sequencing (Part 1)
... an alternative sequencing method. Nobody believed it will ever work • 1991: Light directed polymer synthesis developed by Steve Fodor and colleagues. ...
... an alternative sequencing method. Nobody believed it will ever work • 1991: Light directed polymer synthesis developed by Steve Fodor and colleagues. ...
Chapter 15 - HCC Learning Web
... Around 1902, Walter Sutton, Theodor Boveri, and others noted these parallels and a chromosome theory of inheritance began to take form: Genes occupy specific loci on chromosomes. Chromosomes undergo segregation during meiosis. Chromosomes undergo independent assortment during meiosis. ...
... Around 1902, Walter Sutton, Theodor Boveri, and others noted these parallels and a chromosome theory of inheritance began to take form: Genes occupy specific loci on chromosomes. Chromosomes undergo segregation during meiosis. Chromosomes undergo independent assortment during meiosis. ...
The Large Loop Repair and Mismatch Repair Pathways
... in the RAD1-dependent LLR pathway springs from the known enzymatic roles of those proteins during mitotic DNA repair and the observed effects on meiotic recombination and DNA repair upon deletion of the LLR genes (Kirkpatrick and Petes 1997). Given the characterized activities of Rad1/10p and Msh2/3 ...
... in the RAD1-dependent LLR pathway springs from the known enzymatic roles of those proteins during mitotic DNA repair and the observed effects on meiotic recombination and DNA repair upon deletion of the LLR genes (Kirkpatrick and Petes 1997). Given the characterized activities of Rad1/10p and Msh2/3 ...
Resolution of the type material of the Asian elephant, Elephas
... Sequences of 4258 bp of mtDNA (from MT-ND5 to control region) were aligned for 653 Loxodonta (GenBank accession numbers: JQ438119–JQ438771; Ishida et al., 2013) and 73 E. maximus (unpubl. data). Three short regions that contained genus-diagnostic nucleotide sites were identified, and primers were de ...
... Sequences of 4258 bp of mtDNA (from MT-ND5 to control region) were aligned for 653 Loxodonta (GenBank accession numbers: JQ438119–JQ438771; Ishida et al., 2013) and 73 E. maximus (unpubl. data). Three short regions that contained genus-diagnostic nucleotide sites were identified, and primers were de ...
Resolution of the type material of the Asian elephant, Elephas
... Sequences of 4258 bp of mtDNA (from MT-ND5 to control region) were aligned for 653 Loxodonta (GenBank accession numbers: JQ438119–JQ438771; Ishida et al., 2013) and 73 E. maximus (unpubl. data). Three short regions that contained genus-diagnostic nucleotide sites were identified, and primers were de ...
... Sequences of 4258 bp of mtDNA (from MT-ND5 to control region) were aligned for 653 Loxodonta (GenBank accession numbers: JQ438119–JQ438771; Ishida et al., 2013) and 73 E. maximus (unpubl. data). Three short regions that contained genus-diagnostic nucleotide sites were identified, and primers were de ...
Replisome
The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.