Protocol for Real-Time RT-PCR - MGH-PGA
... Here I listed a few major causes for real-time PCR failures. Please read the PrimerBank Help page for more details. Little or no PCR product. Poor quality of PCR templates, primers, or reagents may lead to PCR failures. First, please include appropriate PCR controls to eliminate these possibilities. ...
... Here I listed a few major causes for real-time PCR failures. Please read the PrimerBank Help page for more details. Little or no PCR product. Poor quality of PCR templates, primers, or reagents may lead to PCR failures. First, please include appropriate PCR controls to eliminate these possibilities. ...
Document
... A group of three bases (a triplet) controls the production of a particular amino acid in the cytoplasm of the cell The different amino acids and the order in which they are joined up determines the sort of protein being produced ...
... A group of three bases (a triplet) controls the production of a particular amino acid in the cytoplasm of the cell The different amino acids and the order in which they are joined up determines the sort of protein being produced ...
Molecular Diagnostics 21
... Single-stranded DNA or RNA molecules used to detect complementary sequences. carry radioactive or chemical markers to facilitate their detection. can be anywhere from fifteen to thousands of nucleotides long. Hybridization reactions using DNA/RNA probes are so sensitive and selective that they can d ...
... Single-stranded DNA or RNA molecules used to detect complementary sequences. carry radioactive or chemical markers to facilitate their detection. can be anywhere from fifteen to thousands of nucleotides long. Hybridization reactions using DNA/RNA probes are so sensitive and selective that they can d ...
RNAzol RT (R4533) - Technical Bulletin - Sigma
... tissue sample. The addition of water to the mixture allows for the precipitation of DNA, proteins, polysaccharides and other molecules, which can be removed by centrifugation. RNA can then be isolated from the supernatant by alcohol precipitation, washing and solubilization. Chloroform-induced phase ...
... tissue sample. The addition of water to the mixture allows for the precipitation of DNA, proteins, polysaccharides and other molecules, which can be removed by centrifugation. RNA can then be isolated from the supernatant by alcohol precipitation, washing and solubilization. Chloroform-induced phase ...
MART TOOTS Novel Means to Target Human Papillomavirus Infection
... cleared by the immune system. The same is true in the case of humans; at some point during their lives, they acquire infections from Human Papillomaviruses (HPVs) that may or may not lead to signs of infection in the form of benign tumors, which will be cleared by the immune system. However, in the ...
... cleared by the immune system. The same is true in the case of humans; at some point during their lives, they acquire infections from Human Papillomaviruses (HPVs) that may or may not lead to signs of infection in the form of benign tumors, which will be cleared by the immune system. However, in the ...
Original Article:
... colonies to find the spa type of the minor population. So far very few studies have focused on this issue. In one study by Cespedes et al. the authors have shown that in nasal carriage less than 10% are colonized by more than one strain. Their approach, cultivation of three colonies from each sample ...
... colonies to find the spa type of the minor population. So far very few studies have focused on this issue. In one study by Cespedes et al. the authors have shown that in nasal carriage less than 10% are colonized by more than one strain. Their approach, cultivation of three colonies from each sample ...
Nucleic acid hybridisation and polymerase chain reaction
... microscopic visualisation, or by detection of biological activity or structural components (antigenic proteins and nucleic acids). The nucleotide sequence of the nucleic acid of each pathogen is different to that of other micro-organisms and susceptible hosts. This uniqueness forms the basis of two ...
... microscopic visualisation, or by detection of biological activity or structural components (antigenic proteins and nucleic acids). The nucleotide sequence of the nucleic acid of each pathogen is different to that of other micro-organisms and susceptible hosts. This uniqueness forms the basis of two ...
The rapidly evolving field of plant centromeres
... DNA in plants, humans, yeast, and Drosophila [28]. If this is the case, even a slight advantage in satellite– CENP-A interactions could quickly result in genomic fixation of satellite arrays. The rapid adaptive evolution of CENP-A observed in both Drosophila and Arabidopsis is consistent with this i ...
... DNA in plants, humans, yeast, and Drosophila [28]. If this is the case, even a slight advantage in satellite– CENP-A interactions could quickly result in genomic fixation of satellite arrays. The rapid adaptive evolution of CENP-A observed in both Drosophila and Arabidopsis is consistent with this i ...
Chapter 18: Gene Mutation and DNA Repair
... a. A change in a single base pair in the DNA. b. A mutation that does not change the amino acid sequence of the polypeptide. c. The addition or deletion of one or two nucleotides. d. A change that alters a single amino acid in the polypeptide. e. A physical change in the structure of the chromosome. ...
... a. A change in a single base pair in the DNA. b. A mutation that does not change the amino acid sequence of the polypeptide. c. The addition or deletion of one or two nucleotides. d. A change that alters a single amino acid in the polypeptide. e. A physical change in the structure of the chromosome. ...
Adaptive value of sex in microbial pathogens
... selects for sex, but only if the fluctuations are rapid (Barton, 1995; Gandon and Otto, 2007). Data from pathogens bearing on the host–parasite model will be discussed below. Because of these and other problems with the negative epistasis model, some workers in this area favor selection and chance e ...
... selects for sex, but only if the fluctuations are rapid (Barton, 1995; Gandon and Otto, 2007). Data from pathogens bearing on the host–parasite model will be discussed below. Because of these and other problems with the negative epistasis model, some workers in this area favor selection and chance e ...
A comparison of the structure of echinomycin and triostin A
... The crystal structure of both complexes was solved by X-ray diffraction to near-atomic resolution. The two structures are similar to each other with differences in some details due to the shorter cross bridge of echinomycin. Both molecules act as bis intercalators surrounding the d(CpG) sequence at ...
... The crystal structure of both complexes was solved by X-ray diffraction to near-atomic resolution. The two structures are similar to each other with differences in some details due to the shorter cross bridge of echinomycin. Both molecules act as bis intercalators surrounding the d(CpG) sequence at ...
Large scale features in DNA genomic signals
... primary structure of a protein is given by the polypeptide chains formed of amino acid sequences. A protein contains one or several such polypeptide chains. The coiling (secondary structure), folding (tertiary structure) and aggregation (quaternary structure) of the polypeptides generate the 7nal ve ...
... primary structure of a protein is given by the polypeptide chains formed of amino acid sequences. A protein contains one or several such polypeptide chains. The coiling (secondary structure), folding (tertiary structure) and aggregation (quaternary structure) of the polypeptides generate the 7nal ve ...
Human RIF1 and protein phosphatase 1 stimulate DNA replication
... RIF1 targeted PP1 acts upon. The authors continue by observing minor defects in DNA replication in RIF1 overexpressing cells and ascribe this observation to reduced helicase activation, yet overexpression of the RIF1-PP1 interaction mutant does not curb this observation as their hypothesis predicts. ...
... RIF1 targeted PP1 acts upon. The authors continue by observing minor defects in DNA replication in RIF1 overexpressing cells and ascribe this observation to reduced helicase activation, yet overexpression of the RIF1-PP1 interaction mutant does not curb this observation as their hypothesis predicts. ...
Replisome
The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.