Supplementary Information (doc 42K)
... between NSOFC and NSCL/P). Note that two additional SNPs overlapping SEMA4D, a gene with roles in axon guidance8, were also included for CLP+CPO replication analysis. Additionally, a gender-determinant site was included in the SNP set to allow confirmation of the maternal and paternal samples. This ...
... between NSOFC and NSCL/P). Note that two additional SNPs overlapping SEMA4D, a gene with roles in axon guidance8, were also included for CLP+CPO replication analysis. Additionally, a gender-determinant site was included in the SNP set to allow confirmation of the maternal and paternal samples. This ...
Appendix A: General Remarks on Handling RNA
... Appendix A: General Remarks on Handling RNA Handling RNA Ribonucleases (RNases) are very stable and active enzymes that generally do not require cofactors to function. Since RNases are difficult to inactivate and even minute amounts are sufficient to destroy RNA, do not use any plasticware or glassw ...
... Appendix A: General Remarks on Handling RNA Handling RNA Ribonucleases (RNases) are very stable and active enzymes that generally do not require cofactors to function. Since RNases are difficult to inactivate and even minute amounts are sufficient to destroy RNA, do not use any plasticware or glassw ...
20Sexual Reproduction, Meiosis, and Genetic Recombination
... Because sexual reproduction combines genetic information from two different parents into a single offspring, at some point in its life cycle every sexually reproducing organism has cells that contain two copies of each type of chromosome, one inherited from each parent. The two members of each chrom ...
... Because sexual reproduction combines genetic information from two different parents into a single offspring, at some point in its life cycle every sexually reproducing organism has cells that contain two copies of each type of chromosome, one inherited from each parent. The two members of each chrom ...
Intelligent Icons: Integrating Lite-Weight Data Mining
... In addition to being able to select both types of Rhinos (Rhinocerotidae) as shown above, we can now also use standard bounding rectangles to select other logical groups, such as: • Both types of elephants (Elephantidae). • All the primates (Catarrhini). • Just the greater apes (Hominidae). • Just t ...
... In addition to being able to select both types of Rhinos (Rhinocerotidae) as shown above, we can now also use standard bounding rectangles to select other logical groups, such as: • Both types of elephants (Elephantidae). • All the primates (Catarrhini). • Just the greater apes (Hominidae). • Just t ...
Structure of Stacked Dimers of N-Methylated Watson–Crick Adenine
... significant slide of one base pair along the y-axis. Such displacement creates favorable conditions for these interactions. The formation of vertical hydrogen bonds can also influence the twist angle between base pairs. The value of the rise parameter (Dz) is unexpectedly small as compared to the st ...
... significant slide of one base pair along the y-axis. Such displacement creates favorable conditions for these interactions. The formation of vertical hydrogen bonds can also influence the twist angle between base pairs. The value of the rise parameter (Dz) is unexpectedly small as compared to the st ...
DNA breathing dynamics distinguish binding from nonbinding
... LMD simulations distinguish true YY1 binding from nonbinding sites in the human PLG promoter YY1 knockdown in HeLa cells coincides with the accumulation of plasminogen (PLG) mRNA (not shown). The gene product regulates a wide variety of biologic responses directly related to the development of cardi ...
... LMD simulations distinguish true YY1 binding from nonbinding sites in the human PLG promoter YY1 knockdown in HeLa cells coincides with the accumulation of plasminogen (PLG) mRNA (not shown). The gene product regulates a wide variety of biologic responses directly related to the development of cardi ...
A Highly Efficient Method for the Construction of a Plasmid
... We have developed a highly efficient method for the construction of a plasmid-based cDNA library. The new method was based on the addition of complementary single stranded oligomers to cDNA and vector as 5' overhangs and annealing of the 5' overhangs before ligation to increase ligation efficiency. ...
... We have developed a highly efficient method for the construction of a plasmid-based cDNA library. The new method was based on the addition of complementary single stranded oligomers to cDNA and vector as 5' overhangs and annealing of the 5' overhangs before ligation to increase ligation efficiency. ...
Vitis 37 (3), 119
... S u m m a r y : Eleven decamer primers of arbitrary nucleotide sequence were used to amplify genomic DNA through the polymerase chain reaction (PCR-RAPD) in order to identify and discriminate between 14 grape cultivars (types or synonyms) belonging to the muscat family. Over 115 reproducible polymor ...
... S u m m a r y : Eleven decamer primers of arbitrary nucleotide sequence were used to amplify genomic DNA through the polymerase chain reaction (PCR-RAPD) in order to identify and discriminate between 14 grape cultivars (types or synonyms) belonging to the muscat family. Over 115 reproducible polymor ...
This article was published in an Elsevier journal. The attached copy
... data from each sample was quantified by manually demarcating regions of interest and analyzed using LivingImage analysis software (Xenogen). 3. Results and discussion 3.1. Generation of VZV ORF deletion mutants In order to test this new VZVluc system for genetic studies, 12 single ORF deletion mutan ...
... data from each sample was quantified by manually demarcating regions of interest and analyzed using LivingImage analysis software (Xenogen). 3. Results and discussion 3.1. Generation of VZV ORF deletion mutants In order to test this new VZVluc system for genetic studies, 12 single ORF deletion mutan ...
OLSON LAB PROTOCOL: Working with RNA
... Purified RNA may be stored at –20°C or –70°C in RNase-free water. Under these conditions, no degradation of RNA is detectable after 1 year. Quantification of RNA The concentration of RNA should be determined by measuring the absorbance at 260 nm (A260) in a spectrophotometer (see “Spectrophotometric ...
... Purified RNA may be stored at –20°C or –70°C in RNase-free water. Under these conditions, no degradation of RNA is detectable after 1 year. Quantification of RNA The concentration of RNA should be determined by measuring the absorbance at 260 nm (A260) in a spectrophotometer (see “Spectrophotometric ...
Highly Efficient Micro RNA Enrichment
... to 40 nucleotides, and the majority of the miRNA is composed of approximately 22 nucleotides. Most of the commercially available miRNA extraction protocols co-purify the miRNA and total RNA. Therefore, the extracted samples still contain ribosomal RNA and messenger RNA with only a low percentage of ...
... to 40 nucleotides, and the majority of the miRNA is composed of approximately 22 nucleotides. Most of the commercially available miRNA extraction protocols co-purify the miRNA and total RNA. Therefore, the extracted samples still contain ribosomal RNA and messenger RNA with only a low percentage of ...
Bacterial Systems for Testing Spontaneous and
... (Janion, 2008). One of the symptoms of the SOS-induction is filamentous growth of bacteria due to expression of the sulA gene. The sulA gene codes for a protein that blocks cell division by inhibiting assembling of the FtsZ protein into a ring structure leading to filament formation (Bi & Lutkenhaus ...
... (Janion, 2008). One of the symptoms of the SOS-induction is filamentous growth of bacteria due to expression of the sulA gene. The sulA gene codes for a protein that blocks cell division by inhibiting assembling of the FtsZ protein into a ring structure leading to filament formation (Bi & Lutkenhaus ...
Rosalind Elsie Franklin Physicist www.AssignmentPoint.com
... articles. On 18 March, in response to receiving a copy of their preliminary manuscript, Wilkins penned the following: "I think you're a couple of old rogues, but you may well have something". ...
... articles. On 18 March, in response to receiving a copy of their preliminary manuscript, Wilkins penned the following: "I think you're a couple of old rogues, but you may well have something". ...
Abstract 1
... We found 10 µM RNA to be an appropriate concentration for screening various small compounds for their ability to form non-covalent complexes. Signals consistent with specific non-covalent binding of neomycin to A-site RNA were observed using both Electrospray and MALDI. An approximately linear relat ...
... We found 10 µM RNA to be an appropriate concentration for screening various small compounds for their ability to form non-covalent complexes. Signals consistent with specific non-covalent binding of neomycin to A-site RNA were observed using both Electrospray and MALDI. An approximately linear relat ...
Hormesis - Illinois Institute of Technology
... Ramsar is a city of about 31,000 people on the north border of Iran, opening on to the Caspian Sea, and is at an elevation of 985m. Nearby hot springs and the rocks emanating from them are full of radium compounds The background levels in some parts of the city are therefore around 10 mGy ~ 100 mSv ...
... Ramsar is a city of about 31,000 people on the north border of Iran, opening on to the Caspian Sea, and is at an elevation of 985m. Nearby hot springs and the rocks emanating from them are full of radium compounds The background levels in some parts of the city are therefore around 10 mGy ~ 100 mSv ...
Using LIMS for Flexible Information Management at a Bovine DNA
... herd improvement in New Zealand and for international dairy farmers. LIC is a cooperative, owned by some 12,500 New Zealand farmers, users of its products and services. LIC’s head office is at Newstead in Hamilton, New Zealand with regional bases around New Zealand and branches in Australia, UK and ...
... herd improvement in New Zealand and for international dairy farmers. LIC is a cooperative, owned by some 12,500 New Zealand farmers, users of its products and services. LIC’s head office is at Newstead in Hamilton, New Zealand with regional bases around New Zealand and branches in Australia, UK and ...
Template for Communications for the Journal of the American
... common property of all the monomeric DNA building blocks: the major part of the excited state population of nucleosides and nucleotides in aqueous solution lives for less than one picosecond.2,3 When applied to double helices, composed exclusively of adenine-thymine base pairs (A-T duplexes, both ho ...
... common property of all the monomeric DNA building blocks: the major part of the excited state population of nucleosides and nucleotides in aqueous solution lives for less than one picosecond.2,3 When applied to double helices, composed exclusively of adenine-thymine base pairs (A-T duplexes, both ho ...
10858_2015_9967_MOESM1_ESM
... S1: DMSO effect on 3’ end transcript homogeneity The effect of DMSO on 3’ end transcript homogeneity was investigated on additional RNA sequences in order to assess the generality of the approach. For transcriptions, standard primers were used to focus on homogeneity effects caused by DMSO and not ...
... S1: DMSO effect on 3’ end transcript homogeneity The effect of DMSO on 3’ end transcript homogeneity was investigated on additional RNA sequences in order to assess the generality of the approach. For transcriptions, standard primers were used to focus on homogeneity effects caused by DMSO and not ...
Reconstruction of the original mycoflora in pelleted feed by PCR
... crossing points from the qPCR of feed samples were converted into log10 CFU g1 feed using a standard curve of A. flavus. Thus, the level of fungal contamination in feed A, B, C, and D is equivalent to 4.79 (standard deviation: r = 0.13), 5.69 (r = 0.03), 5.48 (r = 0.14), and 4.77 (r = 0.05) log10 C ...
... crossing points from the qPCR of feed samples were converted into log10 CFU g1 feed using a standard curve of A. flavus. Thus, the level of fungal contamination in feed A, B, C, and D is equivalent to 4.79 (standard deviation: r = 0.13), 5.69 (r = 0.03), 5.48 (r = 0.14), and 4.77 (r = 0.05) log10 C ...
Diagnostic protocol for
... windows for bacterial cell wall fluorescence, looking for the cells with the size and form of Xac. This procedure permitting detection in the range of 103 cells/ml. Polymerase Chain Reaction (PCR) DNA extraction from infected citrus tissue For obtaining the more accurate PCR results, a DNA extractio ...
... windows for bacterial cell wall fluorescence, looking for the cells with the size and form of Xac. This procedure permitting detection in the range of 103 cells/ml. Polymerase Chain Reaction (PCR) DNA extraction from infected citrus tissue For obtaining the more accurate PCR results, a DNA extractio ...
Replisome
The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.