Replication of plasmids with the p15A origin in Shewanella
... Plasmid pACYC184, a 4·24-kb tetracycline- and chloramphenicol-resistant (Tcr Cmr) cloning vector with the p15A origin of replication (Chang and Cohen 1978), was obtained from New England BioLabs. A 249-bp NcoI-DraI fragment corresponding to a portion of an MR-1 genomic open-reading frame (ORF) that ...
... Plasmid pACYC184, a 4·24-kb tetracycline- and chloramphenicol-resistant (Tcr Cmr) cloning vector with the p15A origin of replication (Chang and Cohen 1978), was obtained from New England BioLabs. A 249-bp NcoI-DraI fragment corresponding to a portion of an MR-1 genomic open-reading frame (ORF) that ...
RECOMBINEERING: A POWERFUL NEW TOOL FOR MOUSE
... the DNA from digestion by RecBCD and provide activation sites on the DNA for RecBCD-mediated recombination in wild-type cells33,34. On encountering a Chi site, the exonuclease activity of RecBCD is attenuated, but because it maintains its helicase activity, it acts on the linear dsDNA to produce a l ...
... the DNA from digestion by RecBCD and provide activation sites on the DNA for RecBCD-mediated recombination in wild-type cells33,34. On encountering a Chi site, the exonuclease activity of RecBCD is attenuated, but because it maintains its helicase activity, it acts on the linear dsDNA to produce a l ...
Direct PCR on Hair: A New Animal
... Figure 4. A 237 bp DNA fragment was amplified from various animal species hair with Phire Tissue Direct PCR Master Mix using both direct and dilution & storage protocol (data with dilution & storage protocol is shown). Identical PCR cycling conditions were used with 3-5 hairs from each animal. NTC – ...
... Figure 4. A 237 bp DNA fragment was amplified from various animal species hair with Phire Tissue Direct PCR Master Mix using both direct and dilution & storage protocol (data with dilution & storage protocol is shown). Identical PCR cycling conditions were used with 3-5 hairs from each animal. NTC – ...
voltammetric methods for determination of some anticancer drugs
... double helical structre of DNA consists of two strands, each of them on the outside of the double helice and formed by altemating phosphate and pentose groups, in which the phosphodiester bridges provide the covalent continuity. The two chains of the double helix are held together by hydrogen bonds ...
... double helical structre of DNA consists of two strands, each of them on the outside of the double helice and formed by altemating phosphate and pentose groups, in which the phosphodiester bridges provide the covalent continuity. The two chains of the double helix are held together by hydrogen bonds ...
Direct PCR on Hair: A New Animal
... Figure 4. A 237 bp DNA fragment was amplified from various animal species hair with Phire Tissue Direct PCR Master Mix using both direct and dilution & storage protocol (data with dilution & storage protocol is shown). Identical PCR cycling conditions were used with 3-5 hairs from each animal. NTC – ...
... Figure 4. A 237 bp DNA fragment was amplified from various animal species hair with Phire Tissue Direct PCR Master Mix using both direct and dilution & storage protocol (data with dilution & storage protocol is shown). Identical PCR cycling conditions were used with 3-5 hairs from each animal. NTC – ...
Geuvadis Analysis Meeting
... Quantification of Splice-Forms and Variants - Quantified 615 datasets based on the Gencode v7 annotation - Sensitivity is a function of sequencing depth ...
... Quantification of Splice-Forms and Variants - Quantified 615 datasets based on the Gencode v7 annotation - Sensitivity is a function of sequencing depth ...
Product Information FluoroVueTM Nucleic Acid Gel Stain (10,000X
... The staining dye can be added into the electrophoresis buffer at a 1:10000 dilution for gel staining during electrophoresis. During/after electrophoresis the gel should be protected from light. The sensitivity of this method is slightly lower than the In-Gel Staining method. 3. Staining after electr ...
... The staining dye can be added into the electrophoresis buffer at a 1:10000 dilution for gel staining during electrophoresis. During/after electrophoresis the gel should be protected from light. The sensitivity of this method is slightly lower than the In-Gel Staining method. 3. Staining after electr ...
Document
... binding site (shown by the red circle). When it is present in the same cell as a wildtype gene, multimeric repressors are assembled at random from both types of subunits. It only requires one of the subunits of the multimer to be of the lacI–d type to block repressor function. This explains the domi ...
... binding site (shown by the red circle). When it is present in the same cell as a wildtype gene, multimeric repressors are assembled at random from both types of subunits. It only requires one of the subunits of the multimer to be of the lacI–d type to block repressor function. This explains the domi ...
Use of a novel cassette to label phenotypically a cryptic plasmid of
... insertion/deletion derivatives. We have identified the replication region as well as separate regions required for segregational and structural stability. The segregational mechanism is very efficient since it allows no detectable loss despite the fact that bacteria carrying the plasmid have a great ...
... insertion/deletion derivatives. We have identified the replication region as well as separate regions required for segregational and structural stability. The segregational mechanism is very efficient since it allows no detectable loss despite the fact that bacteria carrying the plasmid have a great ...
Nucleic Acids: RNA and chemistry
... But it also contains stretches that are not parts of genes at all and are serving controlling or structural roles Avoid the term junk DNA! ...
... But it also contains stretches that are not parts of genes at all and are serving controlling or structural roles Avoid the term junk DNA! ...
Directions for Use Taq DNA Polymerase, 500U
... What is the source of AMRESCO’s UNG? The recombinant cod UNG is produced in an ung negative E. coli strain and is designed to replace the native UNG enzyme originating in Atlantic cod (Gadus morhua). What is the stability of recombinant cod UNG? UNG can tolerate multiple freeze-thaw cycles. It may a ...
... What is the source of AMRESCO’s UNG? The recombinant cod UNG is produced in an ung negative E. coli strain and is designed to replace the native UNG enzyme originating in Atlantic cod (Gadus morhua). What is the stability of recombinant cod UNG? UNG can tolerate multiple freeze-thaw cycles. It may a ...
Mutations
... "inborn errors of metabolism" ((Fig g 7.22)) Beadle and Tatum (1940s) – "the one gene, one enzyme" hypothesis (Fig 7.23) ...
... "inborn errors of metabolism" ((Fig g 7.22)) Beadle and Tatum (1940s) – "the one gene, one enzyme" hypothesis (Fig 7.23) ...
Pearson science 10 Teaching Program 3–4 weeks Chapter 1 DNA
... BIOLOGICAL SCIENCES The transmission of heritable characteristics from one generation to the next involves DNA and genes describing the role of DNA as the blueprint for controlling the characteristics of organisms using models and diagrams to represent the relationship between DNA, genes and chr ...
... BIOLOGICAL SCIENCES The transmission of heritable characteristics from one generation to the next involves DNA and genes describing the role of DNA as the blueprint for controlling the characteristics of organisms using models and diagrams to represent the relationship between DNA, genes and chr ...
PCR primers for the amplification of the nuclear small subunit
... Polycystinea (Radiolaria) is a class of planktonic protists widely distributed in tropical, subtropical, and even polar marine environments. (Currently, many researchers use “Radiolaria” as a conventional name, which comprehensively indicates the Acantharea, the Polycystinea, and the Phaeodarea (Tab ...
... Polycystinea (Radiolaria) is a class of planktonic protists widely distributed in tropical, subtropical, and even polar marine environments. (Currently, many researchers use “Radiolaria” as a conventional name, which comprehensively indicates the Acantharea, the Polycystinea, and the Phaeodarea (Tab ...
bacterial plasmids - Acta Medica Medianae
... of Enterobacteriacae for the very first time. After that, they were discovered in almost every single observed strain. The structure of plasmids is made of circular double chain DNA molecules which are replicated autonomously in a host cell. Their length may vary from few up to several hundred kilob ...
... of Enterobacteriacae for the very first time. After that, they were discovered in almost every single observed strain. The structure of plasmids is made of circular double chain DNA molecules which are replicated autonomously in a host cell. Their length may vary from few up to several hundred kilob ...
The Role of DNA Methylation in Transposable Element Silencing
... Resilencing Dynamics for Active TE Transcription of subsets of TEs activated in mutants deficient in DNA methylation is repressed again when maintenance of DNA methylation is restored. A study using ddm1-derived epiRILs and mutants of RdDM components revealed that transcriptional silencing is achiev ...
... Resilencing Dynamics for Active TE Transcription of subsets of TEs activated in mutants deficient in DNA methylation is repressed again when maintenance of DNA methylation is restored. A study using ddm1-derived epiRILs and mutants of RdDM components revealed that transcriptional silencing is achiev ...
Copy of NAR30_7.book(gkf263.fm)
... moiety, which possesses three dimethoxyltrityl (DMT) protected hydroxyl groups, is used for subsequent thiol functionalization (21). Following removal of the three DMT groups with trichloroacetic acid in methylene chloride, the resulting triol 5 was further coupled with three equivalents of hexyldis ...
... moiety, which possesses three dimethoxyltrityl (DMT) protected hydroxyl groups, is used for subsequent thiol functionalization (21). Following removal of the three DMT groups with trichloroacetic acid in methylene chloride, the resulting triol 5 was further coupled with three equivalents of hexyldis ...
BioACTS Quarter THREE
... a nucleic acid is called a nucleotide. Nucleotides are made up of THREE parts—a phosphate group, a sugar (deoxyribose) and one of four nitrogen bases. Diagram I Draw and label ONE nucleotide using the highlighters below your entry task. ...
... a nucleic acid is called a nucleotide. Nucleotides are made up of THREE parts—a phosphate group, a sugar (deoxyribose) and one of four nitrogen bases. Diagram I Draw and label ONE nucleotide using the highlighters below your entry task. ...
High-Throughput NGS Library Preparation
... and/or cleanup using Agencourt® AMPure® XP beads, you may consider eluting the DNA directly into 1X KAPA End Repair Buffer. These options allow for the addition of up to 65 µl input DNA in 1X KAPA End Repair Buffer in the recommended 70 µl end repair reaction. Another option is to elute fragmented D ...
... and/or cleanup using Agencourt® AMPure® XP beads, you may consider eluting the DNA directly into 1X KAPA End Repair Buffer. These options allow for the addition of up to 65 µl input DNA in 1X KAPA End Repair Buffer in the recommended 70 µl end repair reaction. Another option is to elute fragmented D ...
PDF version - EpiGeneSys
... saturation, and thus chromatin fibre compaction. However, this method consumes a large amount of material, and thus is often not practical.(comment 7) In some circumstances, a thorough analysis of protein content may be necessary ? see (Huynh et al, 2005). This may be the case when working with unus ...
... saturation, and thus chromatin fibre compaction. However, this method consumes a large amount of material, and thus is often not practical.(comment 7) In some circumstances, a thorough analysis of protein content may be necessary ? see (Huynh et al, 2005). This may be the case when working with unus ...
Evidence for Variable Selective Pressures at a
... The increasing amount of available sequences revealed a similar control region structural organization across mammals, with size variation ranging between 880 and 1400 bp (Sbisa et al. 1997). One important issue relating to these observations is thus to identify which structural and/or functional do ...
... The increasing amount of available sequences revealed a similar control region structural organization across mammals, with size variation ranging between 880 and 1400 bp (Sbisa et al. 1997). One important issue relating to these observations is thus to identify which structural and/or functional do ...
Replisome
The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.