Preparation of Lentiviral expression construct DNA
... Standard curve wells: Prepare p53 protein standard according to the instruction in Preparation of reagent. Add 10 μl of p53 standard in duplicate to the standard wells and mix with 50 µl of Binding Buffer A (without DTT). Positive control wells (if protein standard curve is not performed): Add 10 µl ...
... Standard curve wells: Prepare p53 protein standard according to the instruction in Preparation of reagent. Add 10 μl of p53 standard in duplicate to the standard wells and mix with 50 µl of Binding Buffer A (without DTT). Positive control wells (if protein standard curve is not performed): Add 10 µl ...
Classification of Structural Protein Domain Based on Hidden Markov
... [18-23]. However, in many studies, several species were grouped into one category many of or group amino acids into pseudo categories, this will provide less interest on features that are responsible for PDZ domain classifications. Most works in the literatures are focused on predicting various pept ...
... [18-23]. However, in many studies, several species were grouped into one category many of or group amino acids into pseudo categories, this will provide less interest on features that are responsible for PDZ domain classifications. Most works in the literatures are focused on predicting various pept ...
synthesis, chemistry and optical resol
... material-obtainable in optically active form“ and, in principle, easily converted to a 4-[ (carbalkoxy)methyl]cyclopentenone.6a Under the usual Baeyer-Villiger conditions, the product initially formed from norbornenone, lactone 5, rearranged very readily to give the unwanted lactone 6;’however, by u ...
... material-obtainable in optically active form“ and, in principle, easily converted to a 4-[ (carbalkoxy)methyl]cyclopentenone.6a Under the usual Baeyer-Villiger conditions, the product initially formed from norbornenone, lactone 5, rearranged very readily to give the unwanted lactone 6;’however, by u ...
Chapter 25 Alt Notes 0910
... Alcohols and Phenols The functional group in alcohols and phenols is the hydroxyl (-OH) group. Alcohols and phenols can be considered derivatives of hydrocarbons in which one or more H atoms have been replaced by -OH groups. Phenols are derivatives of benzene in which one H has been replaced ...
... Alcohols and Phenols The functional group in alcohols and phenols is the hydroxyl (-OH) group. Alcohols and phenols can be considered derivatives of hydrocarbons in which one or more H atoms have been replaced by -OH groups. Phenols are derivatives of benzene in which one H has been replaced ...
Global MicroRNA Amplification Kit
... B. MicroRNA and Other Small RNAs The study of noncoding RNAs, especially noncoding micro RNAs (miRNA), has gained increasing attention in recent years. Micro RNAs are 19-24 nucleotide long single stranded RNAs that regulate the expression of target genes by interacting with complementary sites in th ...
... B. MicroRNA and Other Small RNAs The study of noncoding RNAs, especially noncoding micro RNAs (miRNA), has gained increasing attention in recent years. Micro RNAs are 19-24 nucleotide long single stranded RNAs that regulate the expression of target genes by interacting with complementary sites in th ...
Exam 3 - Napa Valley College
... mechanism of each reaction. Hint: One step of this reaction produces a very unusual leaving group. 13) Please draw the complete mechanism for the based catalyzed cleavage of an ester. The overall reaction should be, O O ...
... mechanism of each reaction. Hint: One step of this reaction produces a very unusual leaving group. 13) Please draw the complete mechanism for the based catalyzed cleavage of an ester. The overall reaction should be, O O ...
Medical therapy of otitis externa and otitis media Daniel O. Morris, DVM
... typically expressed as minimum inhibitory concentrations (MICs) or reported simply as ‘‘susceptible,’’ ‘‘intermediate,’’ or ‘‘resistant’’ based on the Kirby-Bauer disk diffusion method. For several reasons, this in vitro information may relate poorly to the choice of topical antimicrobial. As already ...
... typically expressed as minimum inhibitory concentrations (MICs) or reported simply as ‘‘susceptible,’’ ‘‘intermediate,’’ or ‘‘resistant’’ based on the Kirby-Bauer disk diffusion method. For several reasons, this in vitro information may relate poorly to the choice of topical antimicrobial. As already ...
Bile Acids and Bile Acid Sequestrants
... The small heterodimer partner (SHP) protein is a member of the nuclear receptor family of intracellular transcription factors and is encoded by the NR0B2 (nuclear receptor subfamily 0, group B, member 2) gene. SHP is unusual for a nuclear receptor in that it lacks a DNA binding domain. Therefore tec ...
... The small heterodimer partner (SHP) protein is a member of the nuclear receptor family of intracellular transcription factors and is encoded by the NR0B2 (nuclear receptor subfamily 0, group B, member 2) gene. SHP is unusual for a nuclear receptor in that it lacks a DNA binding domain. Therefore tec ...
Jon Magnuson, Glenn Fryxell, Linda Lasure, Doug Elliot (PNNL)
... Enzymes are nano-machines of cells, catalyzing thousands of useful chemical reactions. Microscopic reversibility means that outside cells, reactions A --> B and B --> A are feasible. Unlike typical chemical catalysts, enzymatic reactions occur at ambient conditions; i.e. green technology. Enzyme fra ...
... Enzymes are nano-machines of cells, catalyzing thousands of useful chemical reactions. Microscopic reversibility means that outside cells, reactions A --> B and B --> A are feasible. Unlike typical chemical catalysts, enzymatic reactions occur at ambient conditions; i.e. green technology. Enzyme fra ...
Urea Acute Oral Toxicity
... Quick Facts… -Urea can be fed to ruminants as an economical replacement for a part of the protein in a ration. -The amount of urea a ruminant animal can use depends on the digestible energy or total digestible nutrients (TDN) content of the ration. -No more than 45 to 113 grams of urea per head per ...
... Quick Facts… -Urea can be fed to ruminants as an economical replacement for a part of the protein in a ration. -The amount of urea a ruminant animal can use depends on the digestible energy or total digestible nutrients (TDN) content of the ration. -No more than 45 to 113 grams of urea per head per ...
CHAPTER V Fischer-Tropsch Syncrude
... On 22 July 1925 the German scientists Franz Fischer and Hans Tropsch patented a process for the catalytic conversion of carbon monoxide and hydrogen to heavier hydrocarbons. a,(1) Fischer-Tropsch syncrude is a term that is used to collectively describe the products that are obtained by this means. L ...
... On 22 July 1925 the German scientists Franz Fischer and Hans Tropsch patented a process for the catalytic conversion of carbon monoxide and hydrogen to heavier hydrocarbons. a,(1) Fischer-Tropsch syncrude is a term that is used to collectively describe the products that are obtained by this means. L ...
Chemistry Notes for class 12 Chapter 12 Aldehydes, Ketones and
... 1. Aliphatic carboxylic acids up to nine carbon atoms are colourless liquids at room temperature with unpleasant odours. The higher acids are wax like solids. 2. The lower carboxylic acids are freely miscible with water due to the presence of intermolecular hydrogen bonding with H2O molecules. Howev ...
... 1. Aliphatic carboxylic acids up to nine carbon atoms are colourless liquids at room temperature with unpleasant odours. The higher acids are wax like solids. 2. The lower carboxylic acids are freely miscible with water due to the presence of intermolecular hydrogen bonding with H2O molecules. Howev ...
Chapter 11 Structure Determination: Nuclear Magnetic Resonance
... Assigning a Chemical Structure from a 1H NMR Spectrum Propose a structure for a compound, C5H12O, that fits the following 1H NMR data: • 0.92 δ (3 H, triplet, J = 7 Hz) • 1.20 δ (6 H, singlet) • 1.50 δ (2 H, quartet, J = 7 Hz) • 1.64 δ (1H, broad singlet) ...
... Assigning a Chemical Structure from a 1H NMR Spectrum Propose a structure for a compound, C5H12O, that fits the following 1H NMR data: • 0.92 δ (3 H, triplet, J = 7 Hz) • 1.20 δ (6 H, singlet) • 1.50 δ (2 H, quartet, J = 7 Hz) • 1.64 δ (1H, broad singlet) ...
Electrophilic Selenium Catalysis with Electrophilic N
... the requirement. The implementation of this innovation was first documented by Sharpless and co-workers in 1979 [10,11]. In the transformation, PhSeSePh was employed as the pre-catalyst, and underwent the secession of the Se-Se bond to generate an electrophilic species PhSeCl in the presence of N-ch ...
... the requirement. The implementation of this innovation was first documented by Sharpless and co-workers in 1979 [10,11]. In the transformation, PhSeSePh was employed as the pre-catalyst, and underwent the secession of the Se-Se bond to generate an electrophilic species PhSeCl in the presence of N-ch ...
Amino Acids and Proteins
... 28.1A General Features of α-Amino Acids The 20 amino acids that occur naturally in proteins differ in the identity of the R group bonded to the α carbon. The R group is called the side chain of the amino acid. The simplest amino acid, called glycine, has R = H. All other amino acids (R ñ H) have a s ...
... 28.1A General Features of α-Amino Acids The 20 amino acids that occur naturally in proteins differ in the identity of the R group bonded to the α carbon. The R group is called the side chain of the amino acid. The simplest amino acid, called glycine, has R = H. All other amino acids (R ñ H) have a s ...
HMDS - Sigma
... sensitive to moisture and should be handled under dry conditions. Prepare a reagent blank (all components, solvents, etc., except sample), following the same procedure as used for the sample. 1. Weigh 1-10mg of sample into a 5mL reaction vessel. If appropriate, dissolve sample in solvent (see below) ...
... sensitive to moisture and should be handled under dry conditions. Prepare a reagent blank (all components, solvents, etc., except sample), following the same procedure as used for the sample. 1. Weigh 1-10mg of sample into a 5mL reaction vessel. If appropriate, dissolve sample in solvent (see below) ...
Molecular characteristics of sucrose synthase
... growing tissues. The DNA of Arabidopsis thaliana (L.) encodes six SuSy-like genes with distinct expression, down- or up-regulated by various environmental stressors such as anoxia, dehydration, cold treatment and wounding [19-22]. The two ...
... growing tissues. The DNA of Arabidopsis thaliana (L.) encodes six SuSy-like genes with distinct expression, down- or up-regulated by various environmental stressors such as anoxia, dehydration, cold treatment and wounding [19-22]. The two ...
Amines and amides
... found e.g putrescine [H2N(CH2)4NH2] and cadaverine [H2N(CH2)5NH2]! These are the amines responsible for the terrible smell of rotting meat. 2. Key properties: If it smells of “fish” or “rotting flesh” chances are you have an amine!! Amines behave in a similar way to NH3 but their behaviour is modifi ...
... found e.g putrescine [H2N(CH2)4NH2] and cadaverine [H2N(CH2)5NH2]! These are the amines responsible for the terrible smell of rotting meat. 2. Key properties: If it smells of “fish” or “rotting flesh” chances are you have an amine!! Amines behave in a similar way to NH3 but their behaviour is modifi ...
Methodology for the olefination of aldehydes and ketones via the Meyer-Schuster reaction
... Figure 1. Aldol reaction The homologation of aldehydes and ketones to α,β-unsaturated esters (Fig. 1), an indispensable tool for generating carbon–carbon bonds, is typically achieved using aldol condensation1, Wittig, Horner–Wadsworth–Emmons (HWE), or other olefination methods2,3. Of these, the aldo ...
... Figure 1. Aldol reaction The homologation of aldehydes and ketones to α,β-unsaturated esters (Fig. 1), an indispensable tool for generating carbon–carbon bonds, is typically achieved using aldol condensation1, Wittig, Horner–Wadsworth–Emmons (HWE), or other olefination methods2,3. Of these, the aldo ...
Bottromycin
Bottromycin is a macrocyclic peptide with antibiotic activity. It was first discovered in 1957 as a natural product isolated from Streptomyces bottropensis. It has been shown to inhibit methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococci (VRE) among other Gram-positive bacteria and mycoplasma. Bottromycin is structurally distinct from both vancomycin, a glycopeptide antibiotic, and methicillin, a beta-lactam antibiotic.Bottromycin binds to the A site of the ribosome and blocks the binding of aminoacyl-tRNA, therefore inhibiting bacterial protein synthesis. Although bottromycin exhibits antibacterial activity in vitro, it has not yet been developed as a clinical antibiotic, potentially due to its poor stability in blood plasma. To increase its stability in vivo, some bottromycin derivatives have been explored.The structure of bottromycin contains a macrocyclic amidine as well as a thiazole ring. The absolute stereochemistry at several chiral centers has been determined as of 2009. In 2012, a three-dimensional solution structure of bottromycin was published. The solution structure revealed that several methyl groups are on the same face of the structure.Bottromycin falls within the ribosomally synthesized and post-translationally modified peptide class of natural product.