A one-step cloning method for the construction of somatic cell gene
... We used pAAV-LIC to construct SirT1 and HDAC2 KO vector by one-step LIC (Figure 2). The pAAV-LIC vector was digested with EcoRV, resulting in two fragments (approximately 1 kb and 3.1 kb). These fragments were recovered from agarose gels following electrophoresis and purified using gel extraction ki ...
... We used pAAV-LIC to construct SirT1 and HDAC2 KO vector by one-step LIC (Figure 2). The pAAV-LIC vector was digested with EcoRV, resulting in two fragments (approximately 1 kb and 3.1 kb). These fragments were recovered from agarose gels following electrophoresis and purified using gel extraction ki ...
Fertilization & Development
... develop externally in an aquatic environment. The survival rate is generally low, which accounts for the large number of fertilized eggs produced. The developing embryo’s source of food is the yolk stored in the egg. ...
... develop externally in an aquatic environment. The survival rate is generally low, which accounts for the large number of fertilized eggs produced. The developing embryo’s source of food is the yolk stored in the egg. ...
Primer - Workforce Development in Stem Cell Research
... The ability to divide endows stem cells with the capacity to produce large quantities of new cells, sometimes on rather short notice, during embryonic development or when tissues are injured in the adult. They achieve this by performing two types of mitotic cell divisions. The first type is an asymm ...
... The ability to divide endows stem cells with the capacity to produce large quantities of new cells, sometimes on rather short notice, during embryonic development or when tissues are injured in the adult. They achieve this by performing two types of mitotic cell divisions. The first type is an asymm ...
Lecture Note Objectives: Cells Textbook: Chapter 3 Cells and
... Provide/recognize an example for each concept of the Modern Cell Theory. 2) Topic: Cell Size Describe, using cellular structures and their function, how cell size is determined. Explain why smaller cells are more efficient than larger cells. 3) Topic: Fluid Mosaic Model List/describe the general fun ...
... Provide/recognize an example for each concept of the Modern Cell Theory. 2) Topic: Cell Size Describe, using cellular structures and their function, how cell size is determined. Explain why smaller cells are more efficient than larger cells. 3) Topic: Fluid Mosaic Model List/describe the general fun ...
Locus-Specific Control of DNA Methylation by the
... to a cmt3 cytosine MTase mutant (Ebbs et al., 2005). To identify H3 K9 MTases that might account for this residual non-CG methylation, we tested the other seven SUVH proteins encoded in Arabidopsis (Baumbusch et al., 2001) for H3 K9 MTase activity in vitro. The SUVH proteins carry a C-terminal catal ...
... to a cmt3 cytosine MTase mutant (Ebbs et al., 2005). To identify H3 K9 MTases that might account for this residual non-CG methylation, we tested the other seven SUVH proteins encoded in Arabidopsis (Baumbusch et al., 2001) for H3 K9 MTase activity in vitro. The SUVH proteins carry a C-terminal catal ...
the effect of partial protein synthesis inhibition on cell proliferation in
... out with naturally synchronous populations. Bulbs with roots at least 3 cm in length were submerged in a 5 mM caffeine solution for 1 h. This drug inhibits cytokinesis in cells passing through telophase and produces a binucleate cell population, which enters interphase and passes through the whole c ...
... out with naturally synchronous populations. Bulbs with roots at least 3 cm in length were submerged in a 5 mM caffeine solution for 1 h. This drug inhibits cytokinesis in cells passing through telophase and produces a binucleate cell population, which enters interphase and passes through the whole c ...
Biology response 5 yeast
... Schizosaccharomyces pombe: fission yeast has a more typical cell cycle with a long G2. This model organism is therefore a good model for studies of growth control; therefore an excellent model for the cell cycle and cancer initiation and progression. For both yeasts to be used as model organisms the ...
... Schizosaccharomyces pombe: fission yeast has a more typical cell cycle with a long G2. This model organism is therefore a good model for studies of growth control; therefore an excellent model for the cell cycle and cancer initiation and progression. For both yeasts to be used as model organisms the ...
THE CHARACTERIZATION OF ANTIZYME IN DEVELOPING …
... There is clearly a differential expression of antizyme during the various stages of embryonic development. – staining of ring around the blastopore and animal cap – staining of the neural groove, anterior and posterior ends, ventral, and dorsal lateral staining – staining of the somites and anterior ...
... There is clearly a differential expression of antizyme during the various stages of embryonic development. – staining of ring around the blastopore and animal cap – staining of the neural groove, anterior and posterior ends, ventral, and dorsal lateral staining – staining of the somites and anterior ...
PowerPoint - Oregon State University
... Using mutation in general to disrupt gene expression causing mutant phenotype Changing gene expression in only specific cells to locate mutant phenotypes ...
... Using mutation in general to disrupt gene expression causing mutant phenotype Changing gene expression in only specific cells to locate mutant phenotypes ...
What is cloning?
... very different from her mother. The explanation for the difference is that the color and pattern of the coats of cats cannot be attributed exclusively to genes. A biological phenomenon involving inactivation of the X chromosome (See sex chromosome) in every cell of the female cat (which has two X ch ...
... very different from her mother. The explanation for the difference is that the color and pattern of the coats of cats cannot be attributed exclusively to genes. A biological phenomenon involving inactivation of the X chromosome (See sex chromosome) in every cell of the female cat (which has two X ch ...
3. Growth involves both cell division and cell expansion
... these processes is clonal analysis, mapping the cell lineages (clones) derived from each cell in an apical meristem as organs develop. • Researchers induce some change in a cell that tags it in some way such that it (and its descendents) can be distinguished from its neighbors. • For example, a soma ...
... these processes is clonal analysis, mapping the cell lineages (clones) derived from each cell in an apical meristem as organs develop. • Researchers induce some change in a cell that tags it in some way such that it (and its descendents) can be distinguished from its neighbors. • For example, a soma ...
100-Question
... 25. The structures of the components of a nucleotide are illustrated above. The pentose sugars, the phosphate group, and the five nitrogenous bases are depicted. The two molecules that would be found only in DNA, but not RNA, are represented by the letters A. A and D. B. B and E. C. C and F. D. C a ...
... 25. The structures of the components of a nucleotide are illustrated above. The pentose sugars, the phosphate group, and the five nitrogenous bases are depicted. The two molecules that would be found only in DNA, but not RNA, are represented by the letters A. A and D. B. B and E. C. C and F. D. C a ...
Biotechnology - York University
... When the body determines that it requires more of something (e.g. a protein) in a cell, the DNA in that cell opens at the part that has the blueprint on making the protein. A strand of “messenger” RNA forms alongside the opened portion of DNA with the bases that fit against those on the DNA. The RNA ...
... When the body determines that it requires more of something (e.g. a protein) in a cell, the DNA in that cell opens at the part that has the blueprint on making the protein. A strand of “messenger” RNA forms alongside the opened portion of DNA with the bases that fit against those on the DNA. The RNA ...
Functions of DNA methylation: islands, start sites, gene bodies and
... and are marked with trimethylation of histone H3 at lysine 4 (H3K4me3)30 (FIG. 1). The levels of gene expression are controlled by transcription factors31. CGI promoters can be repressed by various mechanisms, such as repression mediated by Polycomb proteins. For example, genes encoding master regul ...
... and are marked with trimethylation of histone H3 at lysine 4 (H3K4me3)30 (FIG. 1). The levels of gene expression are controlled by transcription factors31. CGI promoters can be repressed by various mechanisms, such as repression mediated by Polycomb proteins. For example, genes encoding master regul ...
OVOTESTIS
... when found, can occupy the normal abdominal position, although they may occasionally be found at the internal inguinal ring. Interestingly, ovaries occur more commonly on the left side than the right. The reason for this predilection is unknown. Testes are usually found in the scrotum, although they ...
... when found, can occupy the normal abdominal position, although they may occasionally be found at the internal inguinal ring. Interestingly, ovaries occur more commonly on the left side than the right. The reason for this predilection is unknown. Testes are usually found in the scrotum, although they ...
A2780 is an ovarian cancer cell line
... Cisplatin, or cis-diaminedichloroplatinum the drug most commonly associated with studies involving A2780, is comprised of a doubly charged platinum ion surrounded by four ligands (two amine and two chloride). It is used as an anti-cancer drug. The mode of action for cisplatin is thought to be as a r ...
... Cisplatin, or cis-diaminedichloroplatinum the drug most commonly associated with studies involving A2780, is comprised of a doubly charged platinum ion surrounded by four ligands (two amine and two chloride). It is used as an anti-cancer drug. The mode of action for cisplatin is thought to be as a r ...
Yan D et al., 2014 - Drosophila RNAi Screening Center
... stem cell self-renewal (Spradling et al., 2011; Xie et al., 2008). In the Drosophila ovary, two or three GSCs are located in the most anterior part of the germarium, where they interact with the stem cell niche. A GSC divides asymmetrically to produce another self-renewing GSC and a cystoblast commi ...
... stem cell self-renewal (Spradling et al., 2011; Xie et al., 2008). In the Drosophila ovary, two or three GSCs are located in the most anterior part of the germarium, where they interact with the stem cell niche. A GSC divides asymmetrically to produce another self-renewing GSC and a cystoblast commi ...
supplementary material
... QuantiTect SYBR mix (Qiagen) according to the manufacturer’s instructions. Standard curves were generated using serial 10 fold dilutions of cDNA from J1 mouse embryonic stem cells. Amplification was performed using 40 cycles of denaturation (94°C), annealing (55°C), and extension (72°C). The express ...
... QuantiTect SYBR mix (Qiagen) according to the manufacturer’s instructions. Standard curves were generated using serial 10 fold dilutions of cDNA from J1 mouse embryonic stem cells. Amplification was performed using 40 cycles of denaturation (94°C), annealing (55°C), and extension (72°C). The express ...
Chapter 7/8-Animal Biotechnology
... • Genetically engineered for more rapid growth using the growth hormone gene (salmon, trout, catfish, tuna, etc.) • Genetically engineered for greater disease resistance • Genetically engineered to serve as a biosensor for water pollution ...
... • Genetically engineered for more rapid growth using the growth hormone gene (salmon, trout, catfish, tuna, etc.) • Genetically engineered for greater disease resistance • Genetically engineered to serve as a biosensor for water pollution ...
Full Text - Genes | Genomes | Genetics
... highly informative in many experimental settings, suffers from the stochastic nature of mitotic recombination. This entails tedious work to identify a high enough number of clones in the desired cell type in order to be able to reach a statistically sound conclusion. In addition, phenotypes affecti ...
... highly informative in many experimental settings, suffers from the stochastic nature of mitotic recombination. This entails tedious work to identify a high enough number of clones in the desired cell type in order to be able to reach a statistically sound conclusion. In addition, phenotypes affecti ...
Hym-355 enhances neuron differentiation - Development
... (stage I polyps) were incubated with Hym-355 (10−6 M) for 48 hours. The peptide solution was renewed once at 24 hours. They were pulselabeled with 2 mM BrdU (Sigma) for 1 hour from hour 47 to hour 48 of the peptide treatment, and then immediately macerated into a suspension of single cells (David, 1 ...
... (stage I polyps) were incubated with Hym-355 (10−6 M) for 48 hours. The peptide solution was renewed once at 24 hours. They were pulselabeled with 2 mM BrdU (Sigma) for 1 hour from hour 47 to hour 48 of the peptide treatment, and then immediately macerated into a suspension of single cells (David, 1 ...
A-12 Models for gene activation
... Proposed solution: genes that are less sensitive for the morphogen are better in the autoregulation. In the equation above, this requires with ci+1 > ci ; i = gene number. This condition leads, in addition, to the property that each further step requires a higher signal concentration. Although the s ...
... Proposed solution: genes that are less sensitive for the morphogen are better in the autoregulation. In the equation above, this requires with ci+1 > ci ; i = gene number. This condition leads, in addition, to the property that each further step requires a higher signal concentration. Although the s ...
Activity 3.4.1: Family Inheritance
... That means a child must inherit the gene on chromosomes from both parents. In these cases, both parents must have the gene associated with the trait. Many times neither parent will show the trait because each only has one copy of the gene. When a person has two different alleles for a trait, the per ...
... That means a child must inherit the gene on chromosomes from both parents. In these cases, both parents must have the gene associated with the trait. Many times neither parent will show the trait because each only has one copy of the gene. When a person has two different alleles for a trait, the per ...
Chromosome - s3.amazonaws.com
... A section of DNA that contains coded information as a sequence of bases. Genes code for polypeptides (small proteins). It is these polypeptides that determine the nature and development of organisms. Allele An alternative form of the same gene. Gene e.g. Height – alleles – tall, small. Chromosome A ...
... A section of DNA that contains coded information as a sequence of bases. Genes code for polypeptides (small proteins). It is these polypeptides that determine the nature and development of organisms. Allele An alternative form of the same gene. Gene e.g. Height – alleles – tall, small. Chromosome A ...