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Class Notes On Heredity
Class Notes On Heredity

... result was that the seeds grown from the first cross produced all tall plants. (not a mixture of tall and short plants) --One trait always appeared 100% of the time in the first generation (F1 generation), Mendel called this the dominant trait because it seemed to dominate over the other version of ...
Individual nucleosomes are released by digestion of chromatin with
Individual nucleosomes are released by digestion of chromatin with

... They reform following transcription. Release of H2A·H2B dimers probably initiates the disassembly process. ...
transcription factor
transcription factor

... The Roles of Transcription Factors • To initiate transcription, eukaryotic RNA polymerase requires the assistance of proteins called transcription factors • General transcription factors are essential for the transcription of all protein-coding genes • In eukaryotes, high levels of transcription of ...


... dvances in diagnostic and treatment technologies have resulted in excellent long term survival for Gastric cancer but it is still the second most cause of cancer death in the world.1 About 95% of stomach cancers are of adenocarcinoma type which starts from one of the common cell types found in the l ...
OGT Review Packet Name Period
OGT Review Packet Name Period

... Current Life Science Issues Summarize the historical development of scientific theories and describe emerging issues in the life science field Global Warming – CO2 levels Ozone depletion - CFC’s Cloning/Stem cell research ...
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... Transforming Animal Cells Many egg cells are large enough that DNA can be directly injected into the nucleus. ...
Red Biology guide 235
Red Biology guide 235

... which may be from another organism (for example, the gene for production of human antithrombin III). A transgenic animal is not necessarily a clone or an identical copy of another, although it is possible to make a clone of a transgenic animal. 8. What’s the difference between microinjection and a g ...
Deep Insight Section Hereditary papillary renal cell carcinoma in Oncology and Haematology
Deep Insight Section Hereditary papillary renal cell carcinoma in Oncology and Haematology

... - the Lynch syndrome II. Inheritance: Some familly trees resemble autosomal recessive transmission (affected sibs with unaffected parents), other exhibit typical autosomal dominant trasmission with a vertical parent-to-child pattern; the situation is not that of (recessive) tumour suppressor genes a ...
Enhancers reside in a unique epigenetic environment during early
Enhancers reside in a unique epigenetic environment during early

... The vast majority of enhancers at hypo-methylated loci are not active in any of the embryonic data sets analyzed if we use H3K27ac as a proxy of enhancer activity. To follow up on this observation, we defined two sets of enhancers based on their DNA methylation: hypo-enhancers (<25 % DNA methylation ...
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Example 2. - the Gene Ontology Consortium Wiki

... cultured medium of both MC3T3-E1 cells and primary osteoblasts by BMP-2 ELISA assay (Fig. 2E). These data indicate that rCYR61 induces expression of BMP-2 in osteoblast cells in a time- and dose-dependent manner."... "we hypothesized that, as a positive extracellular signaling protein, CYR61 control ...
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AP Biology Chapter 20 Biotechnology Guided Notes

... Gel Electrophoresis and Southern Blotting • One indirect method of rapidly analyzing and comparing genomes is ___________________ • This technique uses a gel as a _______________ to separate nucleic acids or proteins by ______ ___________________, and other properties • A ________________ is applie ...
Transcription Factors Underlying the Development and Endocrine
Transcription Factors Underlying the Development and Endocrine

... major, terminally differentiated cell types appear: an “invasive trophoblast” cell subtype such as extravillous cytotrophoblast cells in humans and trophoblast giant cells in mice, and a “transport trophoblast” cell subtype that is a syncytium (syncytiotrophoblast) in humans and mice. These two cell ...
Using the hemoglobin switch for the treatment of sickle cell disease
Using the hemoglobin switch for the treatment of sickle cell disease

... gamma chains constitute fetal hemoglobin (HbF). After birth, the switch to adult hemoglobin (HbA) takes place, with only a tiny fraction of HbF remaining in adults. The normal adult hemoglobin tetramer consists of two hemoglobin alpha chains and two hemoglobin beta chains. Mutations in the hemoglobi ...
Epigenetic and genetic factors affect transgene
Epigenetic and genetic factors affect transgene

... In order to account for the segregation of these phenotypes from diploid Fi ova, the expression of this locus must either be subject to allelic exclusion (if expression begins prior to meiosis in the oocyte) or expression must not begin until the completion of meiosis, i.e. after formation of the se ...
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II./ Objectives

... down-regulated upon differentiation of the goblet cell-like HT-29-M6 human colon carcinoma cell line. These transcripts encode proteins originally identified as CROC-1 on the basis of their capacity to activate transcription of c-fos. We show that these proteins are similar in sequence, and in predi ...
Shininger, AnnRev. Plant Physiol. 1979 30:313-37
Shininger, AnnRev. Plant Physiol. 1979 30:313-37

... organisms)into vascular and, in somecases, pith tissue. At a youngstage the cells withinthe central cyclinderdo not haveuniquecytologicalfeatures at the electron microscopelevel whichmightsuggest their early commitmentto different fates (89). In Torrey’sview(131)the ability of the central cylinderto ...
Supporting information for “Dynamics of cell
Supporting information for “Dynamics of cell

... broader than in wild-type. In roots of both wol and cre1 ahk2 ahk3, the AHP6 expression pattern is expanded. Also in the root, the AHP6 transcript is downregulated after a treatment with CKs, and the level of fluorescence in AHP6prom::GFP is also reduced by exogenous CKs. CK (via ARRs) → CKX ...
Insertion of the CCND1 gene into the IgH locus in a case of
Insertion of the CCND1 gene into the IgH locus in a case of

... We identified a unique case of mantle cell lymphoma with leukaemia harbouring a submicroscopic insertion of the CCDN1 gene in the 14q32/IgH locus, producing a hybridisation fusion signal on an apparently normal chromosome 14 and overexpressing cyclin D1 on bone marrow cells. ...
DIFFERENCES IN PLOIDY AND DEGREE OF INTERCELLULAR
DIFFERENCES IN PLOIDY AND DEGREE OF INTERCELLULAR

... usually considered to be possible only immediately following cell division (Sinnott & Bloch, 1945; Fosket, 1968). In some instances, however, differentiation can be induced in parenchyma cells without immediate prior cytokinesis (Sachs, 1969). Fosket & Torrey (1969) have used soybean tissue to show ...
5. Differential Gene Expression
5. Differential Gene Expression

... 8. Enhancers can also inhibit transcription (aka Silencers). ...
Promoter identification and analysis of key glycosphingolipid
Promoter identification and analysis of key glycosphingolipid

... F18. Transcription is initiated by binding of an appropriate RNA polymerase to the promoter region; this process is often stringently regulated for controlling gene expression (Christensen et al., 2004; Li et al., 2015). Analysis of FUT1 gene promoter region revealed that the FUT1 transcription init ...
The Relationship Between XRCC1 and XRCC6 Genes
The Relationship Between XRCC1 and XRCC6 Genes

... Dear Editor, Polymorphisms of genes can cause a decrease in DNA repair capacity and disease susceptibility, as well (1, 2). The XRCC genes play a momentous role in comprehension processes of DNA repair in mammals, especially in doublestrand break (DSB) repair (3). Therefore, normal activity of XRCC ...
DNA Replication Origin Function Is Promoted by H3K4 Di
DNA Replication Origin Function Is Promoted by H3K4 Di

... ABSTRACT DNA replication is a highly regulated process that is initiated from replication origins, but the elements of chromatin structure that contribute to origin activity have not been fully elucidated. To identify histone post-translational modifications important for DNA replication, we initiate ...
Generation of a human induced pluripotent stem cell (iPSC) line
Generation of a human induced pluripotent stem cell (iPSC) line

... performed immunofluorescence staining for the pluripotency transcription factors OCT4 and SOX2, as well as for the hESC surface markers SSEA-3, SSEA-4 and TRA-1-81, which were all expressed in the PDX1 C18R iPSC line (Fig. 2A). To further demonstrate pluripotency of the selected iPSC line we injecte ...
Bookmarking Target Genes in Mitosis: A Shared
Bookmarking Target Genes in Mitosis: A Shared

... incorporation of histone H3.3 variant into genes that are bookmarked for reactivation postmitotically (A), incorporation of histone H2A.Z into nucleosome closer to transcription start sites (TSS) and its sliding on to TSS before mitosis for postmitotic expression (B), maintenance of nuclease accessi ...
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Epigenetics in stem-cell differentiation

Embryonic stem cells are capable of self-renewing and differentiating to the desired fate depending on its position within the body. Stem cell homeostasis is maintained through epigenetic mechanisms that are highly dynamic in regulating the chromatin structure as well as specific gene transcription programs. Epigenetics has been used to refer to changes in gene expression, which are heritable through modifications not affecting the DNA sequence.The mammalian epigenome undergoes global remodeling during early stem cell development that requires commitment of cells to be restricted to the desired lineage. There has been multiple evidence suggesting that the maintenance of the lineage commitment of stem cells are controlled by epigenetic mechanisms such as DNA methylation, histone modifications and regulation of ATP-dependent remolding of chromatin structure. Based on the histone code hypothesis, distinct covalent histone modifications can lead to functionally distinct chromatin structures that influence the fate of the cell.This regulation of chromatin through epigenetic modifications is a molecular mechanism that will determine whether the cell will continue to differentiate into the desired fate. A research study performed by Lee et al. examined the effects of epigenetic modifications on the chromatin structure and the modulation of these epigenetic markers during stem cell differentiation through in vitro differentiation of murine embryonic stem (ES) cells.
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