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Recombinant Human Leukemia Inhibitory Factor (LIF)
Recombinant Human Leukemia Inhibitory Factor (LIF)

... Leukemia Inhibitory Factor also called LIF is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone ...
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... • One from father, one from mother • All body cells Haploid • 1 of each type of chromosome • Only in gametes (reproductive cells) Aneuploid • Wrong number of chromosomes • Extra or missing chromosomes ...
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... Human DNA consists of about 3 billion bases, and more than 99 % of the base sequence in DNA is the same in all people. Less than 1 % is unique to each individual (except identical twins). ...
Inquiry into Life Twelfth Edition
Inquiry into Life Twelfth Edition

... – Oocyte genes are expressed only in oocytes – Somatic genes are expressed both in oocytes and somatic cells – Somatic genes form more stable complexes with transcription factors ...
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Epigenetics in Yeast
Epigenetics in Yeast

... Allele translocation (= gene conversion) between a transcriptionally silent and an active locus, as determined by chromatin structure and epigenetic modifications. The study of this phenomenon, particularly using classical genetics starting in the early ‘80s, has led to the discovery of several fund ...
With or Without them: Essential Roles of Cofactors in ES Cells
With or Without them: Essential Roles of Cofactors in ES Cells

... Page 2 of 9 of ESCs into neurons, skeletal muscles and cardiac myocytes [22-27]. A recent study proposed a model in which phosphorylation of p300 drives the switch from Wnt/β-catenin/CBP-mediated transcription to Wnt/β-catenin/p300-mediated transcription, and initiates the differentiation of mouse E ...
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... mouse ooocytes The piRNA pathway silences retrotransposons and hence maintains genome integrity in the germline. Several components of the piRNA pathway localise to a structure called the nuage, which has been detected in many animal germlines, including mouse testes and Drosophila oocytes. Now, Ai ...
Gene Section CDKN2B (cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4))
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GDR ADN 2014 Chromatin folding in estrogen regulated
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Structure of DNA (Deoxyribonucleic acid)

... as X-rays, UV radiation, and organic chemicals (in cigarette smoke and pesticides). • Effects on organism: may have no effect, be harmful, or result in new beneficial trait • Mutations that prove beneficial make the organism more likely to survive and pass on the new trait to the next generation • I ...
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... The liver and ventral pancreas are thought to develop from a common pool of multipotent progenitors. Although a number of studies have identified factors required for either pancreas or liver specification, factors that are distinctly required to specify the entire hepatopancreas system have not yet ...
Lab Biology Fall Semester Final Exam Study Guide
Lab Biology Fall Semester Final Exam Study Guide

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File - Mrs. Watson`s Homepage

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... several generations in plants. However, in early Lo1E/Lo2 callus decreased expression of the nptII reporter gene was observed while the Lo1/Lo2 remained silenced. Analysis of small RNA species and coding region methylation suggested that the nptII genes were silenced by a PTGS mechanism in both cult ...
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copy number variation, methylation and coregulation in nfkb

... Most of the studied genes that are constitutively upregulated in celiac disease belong to the core of the NFkB route and disruption of coexpression is a relevant feature of the active celiac gut. a) Constitutively overexpressed genes show physical interactions among them and are part of the core of ...
Gene Regulation in Cells
Gene Regulation in Cells

... exact mix of proteins in a cell determines its cell fate (what kind of cell it is, for example making a neuron different from a skin cell). Therefore, gene regulation links genotype (genetic information) and phenotype (observable characteristics). The proteins that control gene regulation are transc ...
Title - Tufts University
Title - Tufts University

... The Wnt family of secreted proteins is essential for normal embryonic development, as well as self renewal and differentiation of adult tissues. Mutations in the Wnt signaling pathway (for example, APC) are well documented in promoting the initiation of colon cancer. Interestingly, mutations in the ...
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L`EQUIPE M3V MODELISATION MULTI - LPTMC

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Recombinant Mouse Leukemia Inhibitory Factor (LIF)
Recombinant Mouse Leukemia Inhibitory Factor (LIF)

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Overview of Articles for the literature talks Nr PMID Titel Date
Overview of Articles for the literature talks Nr PMID Titel Date

... aberrant copy number, sequence, or expression in various cancer types, providing a survey of the genes that may have a causal role in cancer. A complementary approach is to perform systematic loss-of-function studies to identify essential genes in particular cancer cell types. We have begun a system ...
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Figures and figure supplements

... Figure 2. Cells expressing DIS genes are lost following stem cell depletion and express genes associated with the schistosome tegument. (a) Whole-mount in situ hybridization to detect genes expressed in: the intestine (Cathepsin B); neoblasts (fgfrA, nanos2); or cells expressing DIS genes (tsp-2, sm ...
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Overview of Articles for the literature talks Nr PMID Titel Date

... Signaling pathways invoke interplays between forward signaling and feedback to drive robust cellular response. In this study, we address the dynamics of growth factor signaling through profiling of protein phosphorylation and gene expression, demonstrating the presence of a kinetically defined clus ...
Deciphering the interplay between cell wall integrity, sensing and
Deciphering the interplay between cell wall integrity, sensing and

... ligands, respectively. I proceeded to design primers for these T-DNA lines allowing me to PCRgenotype the lines, facilitating the generation of homozygous insertional mutants. These mutants could then be assayed for an abnormal response to ISX treatment, indicating they may function in the same path ...
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Epigenetics in stem-cell differentiation

Embryonic stem cells are capable of self-renewing and differentiating to the desired fate depending on its position within the body. Stem cell homeostasis is maintained through epigenetic mechanisms that are highly dynamic in regulating the chromatin structure as well as specific gene transcription programs. Epigenetics has been used to refer to changes in gene expression, which are heritable through modifications not affecting the DNA sequence.The mammalian epigenome undergoes global remodeling during early stem cell development that requires commitment of cells to be restricted to the desired lineage. There has been multiple evidence suggesting that the maintenance of the lineage commitment of stem cells are controlled by epigenetic mechanisms such as DNA methylation, histone modifications and regulation of ATP-dependent remolding of chromatin structure. Based on the histone code hypothesis, distinct covalent histone modifications can lead to functionally distinct chromatin structures that influence the fate of the cell.This regulation of chromatin through epigenetic modifications is a molecular mechanism that will determine whether the cell will continue to differentiate into the desired fate. A research study performed by Lee et al. examined the effects of epigenetic modifications on the chromatin structure and the modulation of these epigenetic markers during stem cell differentiation through in vitro differentiation of murine embryonic stem (ES) cells.
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