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Comparative sequence analysis of the long repeat regions and
Comparative sequence analysis of the long repeat regions and

... Sequences were compiled using the program set of Staden (1982). Regions presenting problem sequences were resolved using electrophoresis in a 6% polyacrylamidegel containing 9 M-urea, with a water jacket maintained at approximately 80 to 85 °C. Some use was also made of Taq DNA polymerase (Promega) ...
Formal Outline Introduction The Founding of PTC When Who How
Formal Outline Introduction The Founding of PTC When Who How

... slightly bitter, or completely tasteless. Respectively, these people are known as “tasters” and “nontasters.” Approximately 75% of Earth’s population can taste PTC, whereas 25% cannot. PTC was first discovered in 1931 when chemist Arthur L. Fox accidentally released a cloud of finely-powdered PTC in ...
In vitro conjugal transfer of tetracycline resistance from Lactobacillus
In vitro conjugal transfer of tetracycline resistance from Lactobacillus

... include the genera Pediococcus, Enterococcus, Lactobacillus and Leuconostoc but not the genus Lactococcus [23]. Our ¢ndings indicate that the R-plasmids of the investigated Tcr Lactobacillus strains have di¡erent conjugation abilities: some plasmids were transferable to the genera Enterococcus and L ...
Survey of Conserved Alternative Splicing Events
Survey of Conserved Alternative Splicing Events

... To detect AS events, we identified the exon-intron structures of SR protein genes. For Arabidopsis and rice, we identified exon-intron structures by mapping transcripts to the genomes. We mapped transcripts in two steps. First, we roughly mapped transcripts to the genomes using Blast and determined ...
Interphase chromosome profiling (ICP)
Interphase chromosome profiling (ICP)

... showed mosaicism for 45,X[11]/46,X,r(X) [9] karyotype. ICP assay was performed with Xchromosome probes to precisely map the chromosome breaks that led to the development of the r(X). All interphase nuclei (200) evaluated had one copy number for all probes, and evaluation of metaphases did not show s ...
cDNA cloning, expression and chromosomal localization of the
cDNA cloning, expression and chromosomal localization of the

... located on a protrusion of the protein (Eklund et al., 1991). So far, only two thioredoxins have been described in mammalian cells: Trx1 is a cytosolic protein with many different functions most of them depending on the redox active site (Holmgren, 1985) and Trx2 is a mitocondrial protein whose only ...
Coordinated repression and activation of two
Coordinated repression and activation of two

... somatic and visceral muscles (Giesen et al., 1997; Murawsky et al., 2001). We confirmed this observation and, in addition, found that Futsch is also misexpressed in the cardiac mesoderm (supplementary material Fig. S2), suggesting that ttk might be expressed at subdetectable levels in the heart and/ ...
A Haploid System of Sex Determination in the Brown Alga - Hal-CEA
A Haploid System of Sex Determination in the Brown Alga - Hal-CEA

... due, at least in part, to reduced rates of biased gene conversion [31] due to the loss of recombination within the SDR). In addition, transcripts of SDR genes tended to be less abundant on average than transcripts of autosomal genes, although note that codon usage and expression level are likely to ...
Evolution of Mammalian KELL Blood Group Glycoproteins and
Evolution of Mammalian KELL Blood Group Glycoproteins and

... Citation: Holmes RS (2013) Evolution of Mammalian KELL Blood Group Glycoproteins and Genes (KEL): Evidence for a Marsupial Origin from an Ancestral M13 Type II Endopeptidase Gene. J Phylogen Evolution Biol 1: 112. doi:10.4172/2329-9002.1000112 Copyright: © 2013 Holmes RS. This is an open-access arti ...
Regulation of Stage I1 of Sporulation in Bacillus subtilis
Regulation of Stage I1 of Sporulation in Bacillus subtilis

... Stage 0 and stage I represent the vegetative cell, and mutations in a number of genetic loci will prevent further development. These are designated spoOA, spoOB, etc., and it has recently become apparent that most of them, possibly all, are expressed during vegetative growth (Losick et al., 1986; Ya ...
Rice HYDROPEROXIDE LYASES with Unique
Rice HYDROPEROXIDE LYASES with Unique

Genes for two multicopper proteins required for Fe(III) oxide
Genes for two multicopper proteins required for Fe(III) oxide

... Previous studies have shown that Geobacter sulfurreducens requires the outer-membrane, multicopper protein OmpB for Fe(III) oxide reduction. A homologue of OmpB, designated OmpC, which is 36 % similar to OmpB, has been discovered in the G. sulfurreducens genome. Deletion of ompC inhibited reduction ...
Chapter 4.32
Chapter 4.32

... An understanding of the factors that control site occupancy or macroheterogeneity can be useful in controlling N-glycan site occupancy. In recent years, scientists have worked toward (1) regulating the molecular flux in the dolichol pathway, (2) understanding and controlling the function, structural ...
ppt - Central Web Server 2
ppt - Central Web Server 2

... A short introduction to vi is at http://goforit.unk.edu/unix/unix11.htm -- however, if you run into problems google usually helps (e.g. google: vi replace unix gives you many pages of info on how to replace one string with another under vi) ...
4. Microbial Products
4. Microbial Products

... 5-FLOUROURACIL (Uracil analog) p-FLOUROPHENYLALANINE 5-BROMOURACIL (thymine analog) ...
Regulation of Stage I1 of Sporulation in Bacillus subtilis
Regulation of Stage I1 of Sporulation in Bacillus subtilis

... Stage 0 and stage I represent the vegetative cell, and mutations in a number of genetic loci will prevent further development. These are designated spoOA, spoOB, etc., and it has recently become apparent that most of them, possibly all, are expressed during vegetative growth (Losick et al., 1986; Ya ...
Unit 3
Unit 3

... The third letter of the codon is G … So you now you know it is Met (methionine) or start if it’s the first codon of the sequence ...
Adventitious root formation in rice requires OsGNOM1 and
Adventitious root formation in rice requires OsGNOM1 and

... from a cross between a heterozygous gnom1-1 and the indica cultivar Kasalath was used for mapping. AR development was normal in the F1 generation, indicating that a recessive mutation controlled the phenotype. OsGNOM1 was mapped to the long arm of chromosome 3 between the single sequence repeat (SSR ...
Evolution of HSP70 gene and its implications regarding - ACE-SAP
Evolution of HSP70 gene and its implications regarding - ACE-SAP

... other considerations (Lake 1988, 1991; Rivera and Lake 1992). Their recent results indicate that halobacteria and methanogenic archaebacteria show a closer relationship to the eubacteria, whereas extremely thermophilic archaebacteria (termed eocytes) bore sequence characteristics similar to the euka ...
Slide 1
Slide 1

... • For most predictive models this ratio would increase ...
Changes in gene expression associated with acclimation to constant
Changes in gene expression associated with acclimation to constant

Mcp7, a meiosis-specific coiled-coil protein of fission yeast
Mcp7, a meiosis-specific coiled-coil protein of fission yeast

... used to promote cell growth and meiosis has been described previously, as have the standard S.pombe genetic and molecular biology techniques, including northern analysis, which we employed (25). Surface spreading of chromatin was performed according to Bähler et al. (26). Induction of synchronous m ...
Meiosis
Meiosis

... Drosophila, the pairing of homologous chromosomes occurs prior to meiosis. However, in many other organisms such as maize, oat, humans, and mice, homologous chromosomes are not associated with each other until zygotene. Regardless of when chromosomes pair, a major question in meiosis is, how do the ...
Bacteria
Bacteria

... – Useful for differentiating very similar organisms – Hybridization values of 70% or higher suggest strains belong to the same species • Values of at least 25% suggest same genus ...
Incomplete penetrance of MHC susceptibility genes
Incomplete penetrance of MHC susceptibility genes

... There are two main currently available retrospective approaches ...
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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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