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Marfan syndrome in the third Millennium
Marfan syndrome in the third Millennium

... Baldock et al. have derived a model of fibrillin alignment in microfibrils based on automated electron tomography, immunolocalization in directionally orientated untensioned microfibrils, mass changes on microfibril extension, immunofluorescence studies and published observations 28. Their model pre ...
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УДК: 547

... humans when compared with the use of multivariate analysis and mathematical modeling. At the ...
Statistical Selection of Amino Acids Fortifying a Minimal Defined
Statistical Selection of Amino Acids Fortifying a Minimal Defined

... method, the Plackett-Burman design, was used [12]. Each flask contained a certain amino acid predetermined by experimental design (Table 3) with a given amount of glycerol, EDTA, NH4Cl, MgSO4, Na2HPO4, KH2PO4, other metals, and a vitamin solution (Korea patent no.0230580). The concentration of the s ...
Molecular Mechanisms of Developmental Review
Molecular Mechanisms of Developmental Review

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Prof. Kamakaka`s Lecture 1 Notes (PDF)

biochemistry-n-6-protein-metabolism
biochemistry-n-6-protein-metabolism

... converted to urea, and the carbon skeletons are classified as either glucogenic (a precursor of glucose) or ketogenic (a precursor of ketone bodies). • Amino acid catabolism Amino acid catabolism occurs in two main stages. • First stage is the removal of amino group of amino acids as ammonia. Ammoni ...
Vertebrate genomics : More fishy tales about Hox genes
Vertebrate genomics : More fishy tales about Hox genes

... evolutionary lineage leading to zebrafish, some Hox gene clusters must have been duplicated to bring the total number to at least six, two more than had previously been found in any other vertebrate. Amores et al. [9] have now clarified this puzzling situation by their discovery of at least 48 Hox g ...
Regulation of cell fusion in C. elegans - Development
Regulation of cell fusion in C. elegans - Development

... mapping data below) fused to LG II (Meneely and Wood, 1984). All 32/32 male progeny from this cross should be ref-2(mu218)/0; half of these presumably carry the duplication, yet all 32 worms were phenotypically mutant, indicating that a single wild-type copy of the ref-2 locus could not rescue the s ...
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A single-nucleotide polymorphism tagging set for human drug
A single-nucleotide polymorphism tagging set for human drug

... b, Haplotypes. A haplotype is made up of a particular combination of alleles at nearby SNPs. a. For this region, most of the chromosomes in a population survey turn out to have haplotypes 1–4. ...
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2. CYCLIC AMINOACIDS 2.1. Aromatic

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Identification of a putative flexible loop in Arabidopsis glutathione

... protecting plants from environmental stresses [1]. These include, oxidative stress caused by atmospheric air pollutants or excess photosynthetic capacity [2], the toxic effects of xenobiotic organic chemicals [3], and the poisonous effects of some heavy metals [4,5]. A level of resistance to cadmium ...
Saint Patrick High School Curriculum Guide
Saint Patrick High School Curriculum Guide

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Adverse Effects of Excessive Leucine Intake Depend on Dietary
Adverse Effects of Excessive Leucine Intake Depend on Dietary

... provided by Ajinomoto, Co., Inc., Japan). The compositions of the experimental diets are shown in Table 1. After a week of feeding, the rats were anesthetized by intraperitoneal administration of pentobarbital (50 mg/ mL, 100 mL/100 g BW) after 4 h of fasting, and killed. Fresh livers were weighed a ...
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CyO / cn bw let-a?

... Aim: find genes that allow cells to know where they are so the cells can know what they should be expected lof mutant phenotype for “pattern formation” genes: ...
Chapter 11 Mutation: The Source of Genetic Variation
Chapter 11 Mutation: The Source of Genetic Variation

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Functional genomics identifies a Myb domain– containing protein

... content/full/jcb.200701065/DC1). CeCENP-A and KNL-2 are the only centromere/kinetochore proteins identified in C. elegans to date that are present in nuclei throughout the cell cycle. Cumulatively, these results indicate that KNL-2 and CeCENP-A are in close physical proximity on chromatin (and may a ...
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Reduced penetrance in human inherited disease

... ill effects [9]. These variants may damage the protein in question, but the intact protein may not be necessary for the health of the carrier. The individual may be an asymptomatic carrier of a single recessive mutant allele or the mutation is dominant, but the clinical phenotype might be only mild ...
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Comparative sequence analysis of the long repeat regions and

... Sequences were compiled using the program set of Staden (1982). Regions presenting problem sequences were resolved using electrophoresis in a 6% polyacrylamidegel containing 9 M-urea, with a water jacket maintained at approximately 80 to 85 °C. Some use was also made of Taq DNA polymerase (Promega) ...
BIO101 Midterm II Study Guide 03/09/2010
BIO101 Midterm II Study Guide 03/09/2010

... 12. Predict the charge of an atom based upon its neutrons, protons, and electrons. Given an element (ie AL+3) and the atomic number and atomic weight, determine the number of neutrons, protons, and electrons. Similarly, given the number of electrons and protons, give the charge of the atom. Chapter ...
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... • Tumor suppressor genes are the “brakes”. Mutations in these cause loss of function and generally both alleles need to be affected. • Activated oncogenes and loss of tumor suppressor genes cause replication stress and increased DNA damage, which results in tumor progression ...
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... Homologous chromosomes pair up during 1st division and swap portions of chromatids (crossing-over) ...
Lecture 3: Sequence Alignment
Lecture 3: Sequence Alignment

... Compare the gene eyeless of Drosophila Melanoganster with the human gene aniridia. They are master regulatory genes producing proteins that control large cascade of other genes. Certain segments of genes eyeless of Drosophila melanogaster and human aniridia are almost identical. The most important o ...
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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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