• Study Resource
  • Explore Categories
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Sequence Analysis Tools
Sequence Analysis Tools

... – The presence of a gap is ascribed more significance than the length of the gap • A single mutational event may cause the insertion or deletion of more than ...
Fundamentals of Biochemistry 2/e
Fundamentals of Biochemistry 2/e

... Circular Pathway oxidizes acetyl groups from many sources ...
Assiut university researches Some Molecular Biological Studies on
Assiut university researches Some Molecular Biological Studies on

... that RT-PCR followed by RFLP analysis using sac1 for VP2 allowed an accurate classification of a causative agent of our samples as very virulent IBDV. - All examined strains from Assiut province were found to be very virulent IBDV strains. There are changes in nucleotide and aa sequences of the majo ...
Hutational analysis of the influenza virus A/Victoria/3/75 PA protein
Hutational analysis of the influenza virus A/Victoria/3/75 PA protein

... could not be distinguished if the active site of PA, complex additional deletion mutant (PAASA) in the N-terminal half formation or just the general folding of PA was affected by the (A186-280) of PA were analysed (data not shown). The mutation. Thus, we further studied the PA-PB1 association of pol ...
letters - Centre for Social Evolution
letters - Centre for Social Evolution

... sex-determining function by RNAi knockdown experiments. Only csd and the new fem gene, located 12 kb upstream of csd, have sex determination function (Fig. 1b). RNAi-induced knockdowns of fem in females result in a developmental switch to entire male head differentiation (Fig. 1b), whereas knockdown ...
AxyPrep Blood Genomic DNA Midiprep Kit
AxyPrep Blood Genomic DNA Midiprep Kit

... This protocol uses two successive phase-partitioning steps to purify the genomic DNA. In the first step, the addition of Buffer DV facilitates the separation of the lysate into two phases. The genomic DNA is segregated into the lower phase, while lipids, polysaccharides, metabolites and pigments bec ...
Full Text  - American Diabetes Association
Full Text - American Diabetes Association

Multiple Roles of the Y Chromosome in the Biology of
Multiple Roles of the Y Chromosome in the Biology of

... gene, relatively to the others, in any given chromosome. In 1909, Sir A.E. Garrod first pointed out that many human diseases related to recessive traits are due to metabolic defects[7]. However, only in 1941 did the experiments by Beadle and Tatum on Neurospora crassa allow the generalization statin ...
Elimination of Markings - Huzulen im Club Hucul Austria
Elimination of Markings - Huzulen im Club Hucul Austria

... horses where you don’t know whether they are present or absent. Then randomness of mating and unthought and biased selection can induce the loss of genes which stay for very specific and typical attributes - the loss of certain genes may first be in parts of the population, on a long term globally a ...
Mendelian Genetics
Mendelian Genetics

... Individuals with Marfan syndrome tend to be tall and thin with long legs, arms, and fingers; are nearsighted; and the wall of their aorta is weak. d. From his lanky frame and other symptoms, Abraham Lincoln may have had Marfan syndrome. e. Sickle cell anemia causes crystallization of hemoglobin in r ...
Anti-beta-Galactosidase, Rabbit IgG Fraction
Anti-beta-Galactosidase, Rabbit IgG Fraction

... under the supervision of, a technically qualified individual experienced in handling potentially hazardous chemicals. Please read the Material Safety Data Sheet provided for each product; other regulatory considerations may apply. Several of Molecular Probes’ products and product applications are co ...
Gene Section E2F1 (E2F transcription factor 1) Atlas of Genetics and Cytogenetics
Gene Section E2F1 (E2F transcription factor 1) Atlas of Genetics and Cytogenetics

... involved in cell cycle regulation and progression. It is active during the G1 to S transition, and thus its target genes, which include regulatory elements of the cell cycle, such as CDC2, CDC25A and cyclin E, and essential components of DNA replication machinery, including DHFR, and DNA polymerase ...
p53 regulation and function in normal cells and tumors
p53 regulation and function in normal cells and tumors

... genome. p53 is a DNA binding protein; attention has focused on its specific binding to a consensus sequence within promoter regions of growth inhibitory genes. However, p53 also binds sequence-independently to damaged sites in DNA and is postulated to have a role in DNA repair or apoptosis. Mutation ...
Genetics of Asthma – Paul E. Moore MD
Genetics of Asthma – Paul E. Moore MD

... Antigen presentation Fc receptor for IgE a-chain of IL4R,13R ...
Ch. 5 Lecture CH_05_Lecture_v4
Ch. 5 Lecture CH_05_Lecture_v4

... • The polysaccharide cellulose is a major component of the tough wall of plant cells • Like starch, cellulose is a polymer of glucose, but the glycosidic linkages differ • The difference is based on two ring forms for glucose: alpha () and beta () ...
1. Which of the following are connected by hydrogen bonds? A
1. Which of the following are connected by hydrogen bonds? A

... population. The results are shown in pie charts, numbered 1–23, on the map below. The proportions of alleles in a population are called the allele frequencies. The frequency of an allele can vary from 0.0 to 1.0. The light grey sectors of the pie charts show the allele frequency of PanIA and the bla ...
IL-6
IL-6

... Fig (11): Binding interactions of 6f into the active site of p38α (PDB ID: 1GM2). The important amino acid residues are shown together with their respective number. ...
Castric et al. (MBE 2010) - GEPV
Castric et al. (MBE 2010) - GEPV

A Support Vector Machine Approach for LTP Using Amino Acid
A Support Vector Machine Approach for LTP Using Amino Acid

... available. Manual annotation of these sequences is not feasible because data is large. This paper discusses a novel approach of prediction for high-temperature resistant protein LTP using amino acid composition features and hybrid information of proteins. The problem undertaken in this paper is the ...
Perturbations ofDrosophila aActinin Cause Muscle Paralysis
Perturbations ofDrosophila aActinin Cause Muscle Paralysis

... a-Actinin cDNAs were synthesized from staged poly(A)+ RNA preparations of wild-type and liA3 flies using the polymerase chain reaction (PCR) I method . 2 kg of poly(A)+ RNA was denatured by heating to 70°C, annealed to 10 mg/ml of an appropriate antisense oligonucleotide primer, and transcribed usin ...
Journal of Experimental Botany
Journal of Experimental Botany

... Semi-quantitative RT-PCR RNA was extracted from frozen tissues following the method of Logemann et al. (1987), treated with DNase and reverse transcribed with Avian Myeloblastosis virus reverse transcriptase (Finnzymes Oy, Finland) and random hexamer primers. The synthesized cDNAs were ampli®ed usin ...
Product Information Sheet - Sigma
Product Information Sheet - Sigma

... hormone (LH), follicle-stimulating hormone (FSH), and thyroid-stimulating hormone (TSH). Its function is to maintain the corpus luteum and stimulate steroid secretion from the ovary in the beginning stages of gestation. hCG appears in the blood and urine during the first trimester of early pregnancy ...
Genomics Reveal Ancient Forms of Stanniocalcin in Amphioxus and
Genomics Reveal Ancient Forms of Stanniocalcin in Amphioxus and

... Cioin2.home.html) and amphioxus B. floridae (http://genome.jgi-psf.org/Brafl1/Brafl1.home.html) using full-length vertebrate stanniocalcins as queries. One tunicate and two amphioxus orthologs were identified, and primers were designed based on these sequences. Full-length stanniocalcin open reading ...
Technique ChIC and ChEC: Genomic Mapping of Chromatin Proteins
Technique ChIC and ChEC: Genomic Mapping of Chromatin Proteins

... (A) The major steps of the ChIC procedure are illustrated. (Step 1) Binding of the primary antibody (AB) and subsequent centrifugationwashing steps. (Step 2) Binding of the secondary AB and subsequent washing steps. (Step 3) Binding of the fusion protein pA-MN to the secondary AB and final washing s ...
- Bridgewater College WordPress
- Bridgewater College WordPress

... biofilm on medical devices used to treat humans, such as catheters. The real harm comes when cells from the biofilm enter the bloodstream, causing disease and even death (5). Isolate 2 was a Gram positive cocci that occured in tetrads (see Figure 2). Its colony morphology was characterized by circul ...
< 1 ... 281 282 283 284 285 286 287 288 289 ... 2254 >

Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
  • studyres.com © 2025
  • DMCA
  • Privacy
  • Terms
  • Report