Detailed proteome analysis of growing cells of the planctomycete
... combined with nHPLC can be used as an alternative way to identify proteins that cannot be reached by 2-D gel studies. This approach was also used to study cell wall-associated proteins (see Section 3.4). Alternatively we used a gel-free system with multidimensional LC followed by MS. With 329 MS/MS ...
... combined with nHPLC can be used as an alternative way to identify proteins that cannot be reached by 2-D gel studies. This approach was also used to study cell wall-associated proteins (see Section 3.4). Alternatively we used a gel-free system with multidimensional LC followed by MS. With 329 MS/MS ...
Enzymes
... Allosteric Activation and Inhibition • Most allosterically regulated enzymes are made from polypeptide subunits • active and inactive forms • binding of activator stabilizes active form of enzyme • binding of inhibitor stabilizes inactive form of enzyme ...
... Allosteric Activation and Inhibition • Most allosterically regulated enzymes are made from polypeptide subunits • active and inactive forms • binding of activator stabilizes active form of enzyme • binding of inhibitor stabilizes inactive form of enzyme ...
Multidimensional scaling reveals the main evolutionary pathways of class A G-protein-coupled receptors.
... widespread in the animal kingdom and are usually assumed to be G-protein-coupled receptors (GPCRs) by similarity with their vertebrate counterparts. Because they transduce signals from a wide variety of chemical or physical stimuli, these receptors are involved in the perception by the cell of its e ...
... widespread in the animal kingdom and are usually assumed to be G-protein-coupled receptors (GPCRs) by similarity with their vertebrate counterparts. Because they transduce signals from a wide variety of chemical or physical stimuli, these receptors are involved in the perception by the cell of its e ...
Proteomic Analysis of Neisseria gonorrhoeae Biofilms
... microarrays and mass spectrometry-based proteomic technologies. A central question in these studies is how planktonic bacteria alter their gene and protein expression patterns to adapt to biofilm colony formation. In the most heavily studied biofilm organism, Pseudomonas aeruginosa [17,18,19,20], as ...
... microarrays and mass spectrometry-based proteomic technologies. A central question in these studies is how planktonic bacteria alter their gene and protein expression patterns to adapt to biofilm colony formation. In the most heavily studied biofilm organism, Pseudomonas aeruginosa [17,18,19,20], as ...
1- Glycolysis
... cycle or the Krebs cycle: is a series of chemical reactions used by all aerobic organisms to release stored energy through the oxidation of acetyl-CoA derived from carbohydrates, fats and proteins into carbon dioxide and chemical energy in the form of adenosine triphosphate, (ATP.) In addition, the ...
... cycle or the Krebs cycle: is a series of chemical reactions used by all aerobic organisms to release stored energy through the oxidation of acetyl-CoA derived from carbohydrates, fats and proteins into carbon dioxide and chemical energy in the form of adenosine triphosphate, (ATP.) In addition, the ...
Arabidopsis Phosphoglycerate Dehydrogenase1 of
... indicating that the loss of function of PSP or PGDH1 led to embryo lethality (Figure 5D; see Supplemental Figure 5C online). Because we could not obtain homozygous loss-of-function mutants for PGDH1, we generated PGDH1-silenced plants using a microRNA-based approach (Felippes et al., 2012). Transgen ...
... indicating that the loss of function of PSP or PGDH1 led to embryo lethality (Figure 5D; see Supplemental Figure 5C online). Because we could not obtain homozygous loss-of-function mutants for PGDH1, we generated PGDH1-silenced plants using a microRNA-based approach (Felippes et al., 2012). Transgen ...
Nucleotide Catabolism
... Rapidly proliferating cancer cells are voracious consumers of glutamine. They require glutamine for pyrimidine and purine synthesis. Inhibitors of these glutamine amidotransferases have potential as chemotherapeutic agents. Shown to the left are 2 potent inhibitors of glutamine amido transferases, ...
... Rapidly proliferating cancer cells are voracious consumers of glutamine. They require glutamine for pyrimidine and purine synthesis. Inhibitors of these glutamine amidotransferases have potential as chemotherapeutic agents. Shown to the left are 2 potent inhibitors of glutamine amido transferases, ...
Reactive Oxygen Species and Cellular Defense System
... hydrogen atom from another molecule, bind to another molecule, or interact in various ways with other free radicals. Free radicals can be defined as reactive chemical species having a single unpaired electron in an outer orbit and are continuously produced by the organism’s normal use of oxygen [2]. ...
... hydrogen atom from another molecule, bind to another molecule, or interact in various ways with other free radicals. Free radicals can be defined as reactive chemical species having a single unpaired electron in an outer orbit and are continuously produced by the organism’s normal use of oxygen [2]. ...
Chapter 6 Pichia pastoris
... Cytochrome P450s are one of the largest superfamilies of heme-containing enzymes (Chapple, 1998) found in almost all living organisms. Up to now P450s have been found in bacteria, insects, fish, mammals, plants, and fungi (Chapple, 1998). Cinnamic acid 4hydroxylase (C4H) is a cytochrome P450-depende ...
... Cytochrome P450s are one of the largest superfamilies of heme-containing enzymes (Chapple, 1998) found in almost all living organisms. Up to now P450s have been found in bacteria, insects, fish, mammals, plants, and fungi (Chapple, 1998). Cinnamic acid 4hydroxylase (C4H) is a cytochrome P450-depende ...
![[2] Kee HJ*, Eom GH*, Joung H et al., Activation of histone](http://s1.studyres.com/store/data/005630680_1-ca26e8e660c76684aa082bdde0969dfb-300x300.png)
[2] Kee HJ*, Eom GH*, Joung H et al., Activation of histone
... HDAC2. This dissociation leads CK21 to bind to and phosphorylate HDAC2. Hypertrophic stresses induced HSP70 which then preferentially bound to phosphorylated HDAC2 rather than to unphosphorylated one. Forced expression of PPP2CA not only reduced enzyme activity of HDAC2 but inhibited hypertrophic r ...
... HDAC2. This dissociation leads CK21 to bind to and phosphorylate HDAC2. Hypertrophic stresses induced HSP70 which then preferentially bound to phosphorylated HDAC2 rather than to unphosphorylated one. Forced expression of PPP2CA not only reduced enzyme activity of HDAC2 but inhibited hypertrophic r ...
What is the nose?
... • In case of fever, chills, or other signs of systemic infection, avoid giving massage. Is best to delay until this has passed, because a client with inflamed sinuses have trouble in lying flat on a table, especially when faced ...
... • In case of fever, chills, or other signs of systemic infection, avoid giving massage. Is best to delay until this has passed, because a client with inflamed sinuses have trouble in lying flat on a table, especially when faced ...
Promoter Analysis of the Mouse Sterol Regulatory Element
... transcription factors that belong to the basic helix-loop-helix leucine zipper family (1, 2). In contrast to other members of this family, SREBPs are synthesized as precursor proteins that remain bound to the endoplasmic reticulum and the nuclear envelope in the presence of sufficient sterol concent ...
... transcription factors that belong to the basic helix-loop-helix leucine zipper family (1, 2). In contrast to other members of this family, SREBPs are synthesized as precursor proteins that remain bound to the endoplasmic reticulum and the nuclear envelope in the presence of sufficient sterol concent ...
MIBCB Syllabus
... possess fundamental biochemical knowledge and have the ability to use this knowledge most appropriately as applied to clinical requirements, i.e. diagnosis of disease and planning and monitoring of therapy. Apart from providing a competent laboratory service, the clinical chemist must be able to fun ...
... possess fundamental biochemical knowledge and have the ability to use this knowledge most appropriately as applied to clinical requirements, i.e. diagnosis of disease and planning and monitoring of therapy. Apart from providing a competent laboratory service, the clinical chemist must be able to fun ...
An acyltransferase-like gene obtained by differential gene
... consistent with a report that QAs are synthesized in the shoot tissues of L. angustifolius (Lee et al. 2007). A genomic PCR was performed by using the genomic DNA extracted from young leaves of both bitter and sweet cultivars of L. angustifolius (Qiagen DNeasy Plant Mini Kit). Genomic PCR was carrie ...
... consistent with a report that QAs are synthesized in the shoot tissues of L. angustifolius (Lee et al. 2007). A genomic PCR was performed by using the genomic DNA extracted from young leaves of both bitter and sweet cultivars of L. angustifolius (Qiagen DNeasy Plant Mini Kit). Genomic PCR was carrie ...
Chapter 8 – an introduction to metabolism
... 8. Describe how the carbon skeleton of glucose changes as it proceeds through glycolysis. 9. Explain why ATP is required for the preparatory steps of glycolysis. 10. Identify where substrate-level phosphorylation and the reduction of NAD+ occur in glycolysis. 11. Describe where pyruvate is oxidized ...
... 8. Describe how the carbon skeleton of glucose changes as it proceeds through glycolysis. 9. Explain why ATP is required for the preparatory steps of glycolysis. 10. Identify where substrate-level phosphorylation and the reduction of NAD+ occur in glycolysis. 11. Describe where pyruvate is oxidized ...
Principles of BIOCHEMISTRY
... • Galactose from milk lactose (a disaccharide) • Mannose from dietary polysaccharides, glycoproteins ...
... • Galactose from milk lactose (a disaccharide) • Mannose from dietary polysaccharides, glycoproteins ...
Mitochondria-dependent apoptosis and cellular pH regulation
... channel opens, protons and ions equilibrate freely across the inner membrane, and osmotic disequilibrium ensues, resulting in swelling of the matrix space (reviewed in15). Because the inner membrane with its folded cristae has a larger surface area than the surrounding outer membrane, eventually mat ...
... channel opens, protons and ions equilibrate freely across the inner membrane, and osmotic disequilibrium ensues, resulting in swelling of the matrix space (reviewed in15). Because the inner membrane with its folded cristae has a larger surface area than the surrounding outer membrane, eventually mat ...
Di-(2-ethylhexyl) phthalate mediates glycolysis and the TCA cycle
... the two GAPDs indicated that they might be participated in the different metabolic pathways for DEHP detoxication. Mammals were known to possess two tissue-specific GAPD isoenzymes of GAPD-1 and GAPD-2, which served as classical metabolic proteins involved in glycolytic energy production and mainten ...
... the two GAPDs indicated that they might be participated in the different metabolic pathways for DEHP detoxication. Mammals were known to possess two tissue-specific GAPD isoenzymes of GAPD-1 and GAPD-2, which served as classical metabolic proteins involved in glycolytic energy production and mainten ...
- Wiley Online Library
... isomerase activity [44], likewise the specific mRNA transcripts of the respective genes and of glf-specific mRNA were augmented when cells were grown on fructose. Together, this might ensure that sufficient transport protein and enzyme activities are present to cope with the somewhat handicapped fru ...
... isomerase activity [44], likewise the specific mRNA transcripts of the respective genes and of glf-specific mRNA were augmented when cells were grown on fructose. Together, this might ensure that sufficient transport protein and enzyme activities are present to cope with the somewhat handicapped fru ...
Gene Section PKM2 (pyruvate kinase isoenzyme type M2) in Oncology and Haematology
... This leads to an expansion of all phosphometabolites above the pyruvate kinase reaction and an increased channeling of glucose carbons into synthetic processes, i.e. DNA, phospholipid and amino acid synthesis. Tumor cells contain high levels of dimeric M2-PK, which has therefore been termed 'Tumor M ...
... This leads to an expansion of all phosphometabolites above the pyruvate kinase reaction and an increased channeling of glucose carbons into synthetic processes, i.e. DNA, phospholipid and amino acid synthesis. Tumor cells contain high levels of dimeric M2-PK, which has therefore been termed 'Tumor M ...
K-12 MG1655 Escherichia coli Blocks the Aerobic
... RNA was isolated by using RNeasy columns (Qiagen, CA) with DNase I treatment. Total RNA yields were measured by using a spectrophotometer (A260), and quality was checked by visualization on agarose gels and by measurement of the sample A260/A280 ratio (⬎1.8). cDNA preparation was performed as descri ...
... RNA was isolated by using RNeasy columns (Qiagen, CA) with DNase I treatment. Total RNA yields were measured by using a spectrophotometer (A260), and quality was checked by visualization on agarose gels and by measurement of the sample A260/A280 ratio (⬎1.8). cDNA preparation was performed as descri ...
Chapter 20 Notes
... • trans-addition of the elements of water across the double bond • Possible mechanisms are shown in Figure 20.18 • The actual mechanism is not known for certain ...
... • trans-addition of the elements of water across the double bond • Possible mechanisms are shown in Figure 20.18 • The actual mechanism is not known for certain ...