Further studies on the new coomassie brilliant blue G-250 - K-REx
... sensitive than the biuret, requires no digestion of protein and can be completed in about 40 minutes. Finally, an assay which is widely used and depends upon dye binding was introduced by Udy (1954) ...
... sensitive than the biuret, requires no digestion of protein and can be completed in about 40 minutes. Finally, an assay which is widely used and depends upon dye binding was introduced by Udy (1954) ...
Characteristics of Whey Flavor - Innovation Center for US Dairy
... is an important consideration for whey protein ingredients as it is a sensory property of whey proteins, especially in acid conditions. Similar to the issues with off-flavor of whey protein ingredients, astringency of whey protein beverages has been noted as a barrier to mainstream acceptance of hig ...
... is an important consideration for whey protein ingredients as it is a sensory property of whey proteins, especially in acid conditions. Similar to the issues with off-flavor of whey protein ingredients, astringency of whey protein beverages has been noted as a barrier to mainstream acceptance of hig ...
The Unusual Binding Properties of the Third Distinct Teleost
... reproductive, nervous, and cardiovascular systems. Estrogens exert their effects via estrogen receptors (ERs) present in target tissues. Nuclear ERs are members of the steroid/ thyroid/retinoic acid superfamily of ligand-activated transcription factors (1, 2). All members of this family share a modu ...
... reproductive, nervous, and cardiovascular systems. Estrogens exert their effects via estrogen receptors (ERs) present in target tissues. Nuclear ERs are members of the steroid/ thyroid/retinoic acid superfamily of ligand-activated transcription factors (1, 2). All members of this family share a modu ...
Structure of a Pheromone Receptor-Associated MHC Molecule with
... Rich Olson1, Kathryn E. Huey-Tubman1,2, Catherine Dulac3, Pamela J. Bjorkman1,2* 1 Division of Biology, California Institute of Technology, Pasadena, California, United States of America, 2 Howard Hughes Medical Institute, California Institute of Technology, Pasadena, California, United States of Am ...
... Rich Olson1, Kathryn E. Huey-Tubman1,2, Catherine Dulac3, Pamela J. Bjorkman1,2* 1 Division of Biology, California Institute of Technology, Pasadena, California, United States of America, 2 Howard Hughes Medical Institute, California Institute of Technology, Pasadena, California, United States of Am ...
Bacterial ribosome requires multiple L12 dimers for efficient initiation
... and was contradicted by an NMR structure where both the hinges were seen in fully extended form (9,11). Thus it is now universally accepted that the L12 dimer is ‘antiparallel’ where NTDs of two L12 molecules form a four-helix bundle dimer occupying the same site on L10. This model is supported by t ...
... and was contradicted by an NMR structure where both the hinges were seen in fully extended form (9,11). Thus it is now universally accepted that the L12 dimer is ‘antiparallel’ where NTDs of two L12 molecules form a four-helix bundle dimer occupying the same site on L10. This model is supported by t ...
Lessons from the Genome Sequence of Neurospora crassa: Tracing
... Sulfur Metabolism ....................................................................................................................................................47 Sulfur acquisition and processing ................................................................................................. ...
... Sulfur Metabolism ....................................................................................................................................................47 Sulfur acquisition and processing ................................................................................................. ...
Osmo- and thermo-adaptation in hyperthermophilic Archaea
... microorganisms adapted to grow optimally at elevated temperatures tend to use negatively charged solutes that are not present or rarely encountered in mesophilic organisms. In this work, the organic solute pool of several members of the genus Archaeoglobus as well as of the extreme hyperthermophile ...
... microorganisms adapted to grow optimally at elevated temperatures tend to use negatively charged solutes that are not present or rarely encountered in mesophilic organisms. In this work, the organic solute pool of several members of the genus Archaeoglobus as well as of the extreme hyperthermophile ...
THE MULTIFARIOUS AND DYNAMIC REGULATION OF THE LIVING CELL Karen van Eunen
... organisms such that it may harm the organism. In order to survive, the organism should have the ability to rapidly adjust its physiology to the new conditions [110]. For instance, mammals can adapt their physiology such that a constant body temperature is maintained even when the ambient temperature ...
... organisms such that it may harm the organism. In order to survive, the organism should have the ability to rapidly adjust its physiology to the new conditions [110]. For instance, mammals can adapt their physiology such that a constant body temperature is maintained even when the ambient temperature ...
METABOLIC CUES AND REGULATORY PROTEINS
... macrophages to cause the severe pneumonia, Legionnaires’ disease. To persist within these diverse niches, L. pneumophila alternates between at least two distinct phenotypic phases: a non-infectious, replicative form required for intracellular growth and an infectious, transmissive form that enhances ...
... macrophages to cause the severe pneumonia, Legionnaires’ disease. To persist within these diverse niches, L. pneumophila alternates between at least two distinct phenotypic phases: a non-infectious, replicative form required for intracellular growth and an infectious, transmissive form that enhances ...
Bacterial methionine biosynthesis
... the closely related metX genes from Pseudomonas putida and Pseudomonas syringae encode homoserine O-acetyltransferases (Alaminos & Ramos, 2001; Andersen et al., 1998). On balance, it seems likely that the P. aeruginosa homologue is also specific for acetyl-CoA, but in vitro assays will be required t ...
... the closely related metX genes from Pseudomonas putida and Pseudomonas syringae encode homoserine O-acetyltransferases (Alaminos & Ramos, 2001; Andersen et al., 1998). On balance, it seems likely that the P. aeruginosa homologue is also specific for acetyl-CoA, but in vitro assays will be required t ...
The Genera Staphylococcus and Macrococcus
... possibility of false-negative reactions due to PCR inhibitors that might be present in some foods. Several conventional procedures for isolating S. aureus from foods are described below. Nonselective Enrichment Procedures It is often necessary to use nonselective enrichment procedures for the detect ...
... possibility of false-negative reactions due to PCR inhibitors that might be present in some foods. Several conventional procedures for isolating S. aureus from foods are described below. Nonselective Enrichment Procedures It is often necessary to use nonselective enrichment procedures for the detect ...
The Vitamin K-dependent Carboxylase*
... blood proteins have a pro-coagulant function (prothrombin, and factors VII, IX, and X), others primarily serve anti-coagulant roles (proteins C, S, and Z). For all of the VKD blood proteins, however, the Gla residues are located in a homologous ≈ 45 residue amino-terminal “Gla” domain (1-3). The pre ...
... blood proteins have a pro-coagulant function (prothrombin, and factors VII, IX, and X), others primarily serve anti-coagulant roles (proteins C, S, and Z). For all of the VKD blood proteins, however, the Gla residues are located in a homologous ≈ 45 residue amino-terminal “Gla” domain (1-3). The pre ...
principles of metabolic regulation: glucose and glycogen
... Chapter 14 we discussed three possible fates for glucose 6-phosphate in a hepatocyte: passage into glycolysis for the production of ATP, passage into the pentose phosphate pathway for the production of NADPH and pentose phosphates, or hydrolysis to glucose and phosphate to replenish blood glucose. I ...
... Chapter 14 we discussed three possible fates for glucose 6-phosphate in a hepatocyte: passage into glycolysis for the production of ATP, passage into the pentose phosphate pathway for the production of NADPH and pentose phosphates, or hydrolysis to glucose and phosphate to replenish blood glucose. I ...
Functional genomics analysis of the effects of co- decarboxylase/ornithine decarboxylase
... Plasmodium falciparum, polyamine biosynthesis is regulated by the uniquely bifunctional protein, Sadenosylmethionine decarboxylase/ornithine decarboxylase (PfAdoMetDC/ODC). The unique nature of this protein could provide a selective mechanism for antimalarial treatment. To validate polyamine depleti ...
... Plasmodium falciparum, polyamine biosynthesis is regulated by the uniquely bifunctional protein, Sadenosylmethionine decarboxylase/ornithine decarboxylase (PfAdoMetDC/ODC). The unique nature of this protein could provide a selective mechanism for antimalarial treatment. To validate polyamine depleti ...
Myr 8, A Novel Unconventional Myosin Expressed during Brain
... neurons exit the cell cycle within the ventricular zone and subsequently initiate an active cell movement along a scaffolding of radial glial cell processes to settle in the cortical plate (Sidman and Rakic, 1973; Rakic, 1990; Hatten, 1999). A lesser population of cortical neurons arise in either th ...
... neurons exit the cell cycle within the ventricular zone and subsequently initiate an active cell movement along a scaffolding of radial glial cell processes to settle in the cortical plate (Sidman and Rakic, 1973; Rakic, 1990; Hatten, 1999). A lesser population of cortical neurons arise in either th ...
Major players on the microbial stage: why archaea
... eukaryal features with unique archaeal ones to create a third functioning mechanism. Archaea are useful model systems for processes in both eukarya and bacteria, and their major roles in various ecosystems, not just extremophilic ones, continue to be uncovered. They have useful biotechnology/ commer ...
... eukaryal features with unique archaeal ones to create a third functioning mechanism. Archaea are useful model systems for processes in both eukarya and bacteria, and their major roles in various ecosystems, not just extremophilic ones, continue to be uncovered. They have useful biotechnology/ commer ...
Transcription factor Sp3 is silenced through - IMT
... GC box present in many promoters. The expression pattern, the structure and the DNA-binding capacity of Sp3 are very similar to Sp1. The physiological role of Sp1 and Sp3, however, appears to be different since Sp3- and Sp1de®cient mice exhibit different phenotypes (Marin et al., 1997; Bouwman et al ...
... GC box present in many promoters. The expression pattern, the structure and the DNA-binding capacity of Sp3 are very similar to Sp1. The physiological role of Sp1 and Sp3, however, appears to be different since Sp3- and Sp1de®cient mice exhibit different phenotypes (Marin et al., 1997; Bouwman et al ...
Chapter 4 General metabolism
... hexose. For instance, glucokinase has a high specificity for glucose and very low, or non-existent, specificity for other monosaccharides. S. cerevisiae is known to possess two hexokinases and a glucokinase, encoded by HXK1, HXK2 and GLK1, respectively [Lobo and Maitra, 1977]. Hexokinases were also ...
... hexose. For instance, glucokinase has a high specificity for glucose and very low, or non-existent, specificity for other monosaccharides. S. cerevisiae is known to possess two hexokinases and a glucokinase, encoded by HXK1, HXK2 and GLK1, respectively [Lobo and Maitra, 1977]. Hexokinases were also ...
Trichomonas vaginalis - Università degli Studi di Sassari
... Trichomoniasis is the most common non-viral, curable, STD worldwide that annually affects millions of people[1]. The causative agent of infection is Trichomonas vaginalis, obligate extracellular mucosal parasite that induce significant health sequelae in both men and women. In women, symptoms range ...
... Trichomoniasis is the most common non-viral, curable, STD worldwide that annually affects millions of people[1]. The causative agent of infection is Trichomonas vaginalis, obligate extracellular mucosal parasite that induce significant health sequelae in both men and women. In women, symptoms range ...
Structural and Functional Studies on Proteinaceous Metallocarboxypeptidase Inhibitors Joan López Arolas
... Peptidase inhibitors Peptidase inhibitors are important tools of nature for regulating the proteolytic activity of their target proteases, and for blocking them in emergency cases (Bode & Huber, 1992). All naturally occurring inhibitors directed toward endogenous peptidases, i.e. against proteolytic ...
... Peptidase inhibitors Peptidase inhibitors are important tools of nature for regulating the proteolytic activity of their target proteases, and for blocking them in emergency cases (Bode & Huber, 1992). All naturally occurring inhibitors directed toward endogenous peptidases, i.e. against proteolytic ...
ABA overlysensitive5 (ABO5), encoding a pentatricopeptide repeat
... Journal compilation ª 2010 Blackwell Publishing Ltd, The Plant Journal, (2010), 63, 749–765 ...
... Journal compilation ª 2010 Blackwell Publishing Ltd, The Plant Journal, (2010), 63, 749–765 ...
Being right on Q: shaping eukaryotic evolution
... formation is not a peripheral bacterial phenomenon anymore: it now occurs in the middle of the new organism. Secondly, alternatively using different catabolic substrates, as described above, gave rise to more ROS formation [5,6]. Why should this be so? Relative amounts of the intermediates FADH2 and ...
... formation is not a peripheral bacterial phenomenon anymore: it now occurs in the middle of the new organism. Secondly, alternatively using different catabolic substrates, as described above, gave rise to more ROS formation [5,6]. Why should this be so? Relative amounts of the intermediates FADH2 and ...
Glycerol transport and phosphoenolpyruvate
... and metabolism independent of PTS-mediated activation by phosphorylation. This could indeed be confirmed for the His-232-Arg mutant glycerol kinase of Ent. casseliflavus, which exhibited much higher activity than the wild-type enzyme (Charrier et al., 1997). An amino acid sequence comparison with kn ...
... and metabolism independent of PTS-mediated activation by phosphorylation. This could indeed be confirmed for the His-232-Arg mutant glycerol kinase of Ent. casseliflavus, which exhibited much higher activity than the wild-type enzyme (Charrier et al., 1997). An amino acid sequence comparison with kn ...
Queuosine formation in eukaryotic tRNA occurs via a mitochondria
... into the NdeI and XhoI restriction sites of the pET21a plasmid (Novagen) to produce the plasmid Qv1:pET21a (His-tag). A truncated version of the mouse TGT (tTGT) was also produced, missing the coding sequence for the first 16 amino acids. This sequence was amplified from the fTGT:pGEX6P1 plasmid usi ...
... into the NdeI and XhoI restriction sites of the pET21a plasmid (Novagen) to produce the plasmid Qv1:pET21a (His-tag). A truncated version of the mouse TGT (tTGT) was also produced, missing the coding sequence for the first 16 amino acids. This sequence was amplified from the fTGT:pGEX6P1 plasmid usi ...
Scientific Opinion on Dietary Reference Values for protein
... of dietary protein needed for milk protein production. As the efficiency of protein utilisation for milk protein production is unknown, the same efficiency as in the non-lactating adult (47 %) was assumed. The PRI was estimated by adding 1.96 SD to give an additional 19 g protein/d during the first ...
... of dietary protein needed for milk protein production. As the efficiency of protein utilisation for milk protein production is unknown, the same efficiency as in the non-lactating adult (47 %) was assumed. The PRI was estimated by adding 1.96 SD to give an additional 19 g protein/d during the first ...
Paracrine signalling
Paracrine signaling is a form of cell-cell communication in which a cell produces a signal to induce changes in nearby cells, altering the behavior or differentiation of those cells. Signaling molecules known as paracrine factors diffuse over a relatively short distance (local action), as opposed to endocrine factors (hormones which travel considerably longer distances via the circulatory system), juxtacrine interactions, and autocrine signaling. Cells that produce paracrine factors secrete them into the immediate extracellular environment. Factors then travel to nearby cells in which the gradient of factor received determines the outcome. However, the exact distance that paracrine factors can travel is not certain.Although paracrine signaling elicits a diverse array of responses in the induced cells, most paracrine factors utilize a relatively streamlined set of receptors and pathways. In fact, different organs in the body -even between different species - are known to utilize a similar sets of paracrine factors in differential development. The highly conserved receptors and pathways can be organized into four major families based on similar structures: Fibroblast growth factor (FGF) family, Hedgehog family, Wnt family, and TGF-β superfamily. Binding of a paracrine factor to its respective receptor initiates signal transduction cascades, eliciting different responses.