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Asgeirsson, B., Renzetti, G., Invernizzi, G ., Papaleo, E. (2013)
... cold-active alkaline phosphatase (VAP)1. Asymmetric dynamics have been suggested to play a part in the catalytic cycle in homodimeric alkaline phosphatases. A conformational change might be the rate-limiting step since the chemical transformations are much faster than kcat2. Asymmetric protein dynam ...
... cold-active alkaline phosphatase (VAP)1. Asymmetric dynamics have been suggested to play a part in the catalytic cycle in homodimeric alkaline phosphatases. A conformational change might be the rate-limiting step since the chemical transformations are much faster than kcat2. Asymmetric protein dynam ...
ProRule: a new database containing functional
... physico-chemical properties associated with them, the proper reaction cannot take place and the function or structure will be altered. The fact that profiles generally cover domains or proteins over their entire length makes the identification of such biologically relevant residues less obvious than ...
... physico-chemical properties associated with them, the proper reaction cannot take place and the function or structure will be altered. The fact that profiles generally cover domains or proteins over their entire length makes the identification of such biologically relevant residues less obvious than ...
CONFERENCE REPORT FROM A NOBEL MINISYMPOSIUM/JIM
... their native structures into amyloid fibrils, and in line with those observations almost all proteins apparently contain segments with the potential to form amyloid-like fibrils. However, only a tiny fraction of all proteins really forms amyloid - so far only 30 amyloid diseases have been described, ...
... their native structures into amyloid fibrils, and in line with those observations almost all proteins apparently contain segments with the potential to form amyloid-like fibrils. However, only a tiny fraction of all proteins really forms amyloid - so far only 30 amyloid diseases have been described, ...
Proteomics insights: proteins related to larval attachment and
... to as “spontaneous metamorphosis.” Although past studies have contributed to our understanding of the basic underpinnings of the morphological, ecological and behavioral patterns of larval settlement, the molecular mechanisms that regulate larval attachment and metamorphosis are poorly understood. I ...
... to as “spontaneous metamorphosis.” Although past studies have contributed to our understanding of the basic underpinnings of the morphological, ecological and behavioral patterns of larval settlement, the molecular mechanisms that regulate larval attachment and metamorphosis are poorly understood. I ...
Quantitative Comparison of the Hydrogen Bond
... globular than the native state. Nevertheless, certain secondary elements can be clearly detected. In the N-terminal part, the first antiparallel β-hairpin β1/β2 (residues M1 to V17) and the central R-helix R (residues I23 to G35) of the native state preserve their secondary conformation in the A-sta ...
... globular than the native state. Nevertheless, certain secondary elements can be clearly detected. In the N-terminal part, the first antiparallel β-hairpin β1/β2 (residues M1 to V17) and the central R-helix R (residues I23 to G35) of the native state preserve their secondary conformation in the A-sta ...
Disulfide formation in plant storage vacuoles permits assembly
... compost and vermiculite under greenhouse conditions at 15°C with a 16 hour light / 8 hour dark cycle. Prior to planting, seeds were imbibed in running water overnight. The development of Ricinus communis seeds is divided into seven stages (A to G) based on size, testa formation and state of hydratio ...
... compost and vermiculite under greenhouse conditions at 15°C with a 16 hour light / 8 hour dark cycle. Prior to planting, seeds were imbibed in running water overnight. The development of Ricinus communis seeds is divided into seven stages (A to G) based on size, testa formation and state of hydratio ...
Affinity Chromatography
... use of CNBr activation chemistry was a milestone in the development of the technique, because the complex organic chemistry required for the synthesis of reliable immobilized ligand matrices had previously prevented this technique from becoming generally established in biological laboratories. Fourt ...
... use of CNBr activation chemistry was a milestone in the development of the technique, because the complex organic chemistry required for the synthesis of reliable immobilized ligand matrices had previously prevented this technique from becoming generally established in biological laboratories. Fourt ...
Supplementary Figure 2. Further examples of large differences
... 43, 44, 42, 41 and 23. The MCC score for this prediction is 0.127 however the BDT score (with d0=3Å) is 0.436. Target T0480 has two disordered regions at the ends of the protein and if these regions are excluded from the MCC calculation then the MCC score is further reduced to 0.055 but the BDT scor ...
... 43, 44, 42, 41 and 23. The MCC score for this prediction is 0.127 however the BDT score (with d0=3Å) is 0.436. Target T0480 has two disordered regions at the ends of the protein and if these regions are excluded from the MCC calculation then the MCC score is further reduced to 0.055 but the BDT scor ...
Articulins and epiplasmins - Journal of Cell Science
... epiplasm generally reveals many different molecular mass bands, with a pronounced interspecific variation in banding pattern, even among closely related species that are nearly identical morphologically (see, for example, Williams et al., 1984). Polyclonal and monoclonal antibodies (mAbs) directed a ...
... epiplasm generally reveals many different molecular mass bands, with a pronounced interspecific variation in banding pattern, even among closely related species that are nearly identical morphologically (see, for example, Williams et al., 1984). Polyclonal and monoclonal antibodies (mAbs) directed a ...
The Evolution of SMC Proteins: Phylogenetic Analysis and Structural
... gene duplication events, with the condensins evolving more rapidly than the cohesins. In addition, we show that the SMC5 and SMC6 subfamily members have evolved comparatively rapidly and suggest that these proteins may perform redundant functions in higher eukaryotes. Finally, we propose a possible ...
... gene duplication events, with the condensins evolving more rapidly than the cohesins. In addition, we show that the SMC5 and SMC6 subfamily members have evolved comparatively rapidly and suggest that these proteins may perform redundant functions in higher eukaryotes. Finally, we propose a possible ...
a healthy diet for an athlete includes the proper ratio of simple carbs
... percentage of proteins would cause significant damage to your liver. People who are on a high protein diet for a prolonged period of time should be cautious about this. Your body can convert proteins into fat in your body, but generally won't unless there are no complex or simple carbs presant. One ...
... percentage of proteins would cause significant damage to your liver. People who are on a high protein diet for a prolonged period of time should be cautious about this. Your body can convert proteins into fat in your body, but generally won't unless there are no complex or simple carbs presant. One ...
Infectious Salmon Anaemia Virus (ISAV) RNA Binding Protein
... the AMAXA™ Nucleofector™ (Lonza, Basel, Switzerland) and Ingenio transfection reagents (Mirus Bio, Madison, WI, USA). This construct was made by cloning the Glesvaer s8ORF2 gene (Genbank acc. no. DQ785276) into the EcoRI/XhoI restriction sites of the pcDNA3.1+Myc-His vector C (Invitrogen Thermo-Fish ...
... the AMAXA™ Nucleofector™ (Lonza, Basel, Switzerland) and Ingenio transfection reagents (Mirus Bio, Madison, WI, USA). This construct was made by cloning the Glesvaer s8ORF2 gene (Genbank acc. no. DQ785276) into the EcoRI/XhoI restriction sites of the pcDNA3.1+Myc-His vector C (Invitrogen Thermo-Fish ...
2-D Quant Kit - GE Healthcare Life Sciences
... The kit contains reagents sufficient for 500 data points. Warning For research use only. Not recommended or intended for diagnosis of disease in humans or animals. Do not use internally or externally in humans or animals. ...
... The kit contains reagents sufficient for 500 data points. Warning For research use only. Not recommended or intended for diagnosis of disease in humans or animals. Do not use internally or externally in humans or animals. ...
perspective - Pseudomonas syringae Genome Resources Home Page
... whether the peptidoglycan degradation products interact directly with the LRRs of Nod1, Nod2 or NALP3 or whether there is a linker protein involved. In any case, peptidoglycan must translocate into the cytoplasm, and at least some cell types such as macrophages have intracellular hydrolyases that ca ...
... whether the peptidoglycan degradation products interact directly with the LRRs of Nod1, Nod2 or NALP3 or whether there is a linker protein involved. In any case, peptidoglycan must translocate into the cytoplasm, and at least some cell types such as macrophages have intracellular hydrolyases that ca ...
Bonding and structure
... instructions are readily available on the internet using cheap materials. Colleagues who teach biology may also have models of DNA. It is important to stress that although the DNA molecule is very large, the important feature that allows replication is the hydrogen bonds between base pairs. ...
... instructions are readily available on the internet using cheap materials. Colleagues who teach biology may also have models of DNA. It is important to stress that although the DNA molecule is very large, the important feature that allows replication is the hydrogen bonds between base pairs. ...
Facing extremes: archaeal surface-layer (glyco)proteins
... survival in the face of such environmental challenges. One aspect of such investigation asks how the archaeal cell envelope, directly exposed to the harsh physical conditions in which these micro-organisms exist, manages to maintain its structural integrity. In most cases, a surface (S)-layer, gener ...
... survival in the face of such environmental challenges. One aspect of such investigation asks how the archaeal cell envelope, directly exposed to the harsh physical conditions in which these micro-organisms exist, manages to maintain its structural integrity. In most cases, a surface (S)-layer, gener ...
PDF + SI - GenScript
... bromide and MNase to remove any contaminating DNA. As shown in Fig. 1B and Fig. 6, which is published as supporting information on the PNAS web site, Cdc45 coimmunoprecipitates with 10 other major proteins. The identity of the copurifying proteins was determined by two methods: (i) mass spectroscopy ...
... bromide and MNase to remove any contaminating DNA. As shown in Fig. 1B and Fig. 6, which is published as supporting information on the PNAS web site, Cdc45 coimmunoprecipitates with 10 other major proteins. The identity of the copurifying proteins was determined by two methods: (i) mass spectroscopy ...
Crystal structure of plant photosystem I
... are positioned in strategic locations in the cleft between LHCI and the core. This structure provides a framework for exploration not only of energy and electron transfer but also of the evolutionary forces that shaped the photosynthetic apparatus of terrestrial plants after the divergence of chloro ...
... are positioned in strategic locations in the cleft between LHCI and the core. This structure provides a framework for exploration not only of energy and electron transfer but also of the evolutionary forces that shaped the photosynthetic apparatus of terrestrial plants after the divergence of chloro ...
program
... Subdomain F3: Detecting the presence of substances The candidate can list a number of methods used to detect the presence of substances and interpret the results that are thus obtained. The candidate can: 139 interpret paper and thin Iayer chromatograms for the purpose of recognising substances. 140 ...
... Subdomain F3: Detecting the presence of substances The candidate can list a number of methods used to detect the presence of substances and interpret the results that are thus obtained. The candidate can: 139 interpret paper and thin Iayer chromatograms for the purpose of recognising substances. 140 ...
Allosteric regulation and catalysis emerge via a common
... a limited number of residues. Also, though they yield interesting information, it is difficult to probe the existence of an entire ‘channel’ or network of amino acids through which allosteric signals are communicated. An alternative approach developed by Rama Ranganathan and colleagues uses a sequen ...
... a limited number of residues. Also, though they yield interesting information, it is difficult to probe the existence of an entire ‘channel’ or network of amino acids through which allosteric signals are communicated. An alternative approach developed by Rama Ranganathan and colleagues uses a sequen ...
Crystal structure of plant photosystem I
... are positioned in strategic locations in the cleft between LHCI and the core. This structure provides a framework for exploration not only of energy and electron transfer but also of the evolutionary forces that shaped the photosynthetic apparatus of terrestrial plants after the divergence of chloro ...
... are positioned in strategic locations in the cleft between LHCI and the core. This structure provides a framework for exploration not only of energy and electron transfer but also of the evolutionary forces that shaped the photosynthetic apparatus of terrestrial plants after the divergence of chloro ...
Methods S1.
... net/viewvc/sashimi/). Assignment of MS2 spectra was performed using the Sequest algorithm by searching the data against a protein sequence database containing all known translated proteins from either Mtb or Msm, and known contaminants such as porcine trypsin, and human keratin[3]. This forward (tar ...
... net/viewvc/sashimi/). Assignment of MS2 spectra was performed using the Sequest algorithm by searching the data against a protein sequence database containing all known translated proteins from either Mtb or Msm, and known contaminants such as porcine trypsin, and human keratin[3]. This forward (tar ...
Proteases and proteolytic cleavage of storage
... whereas the smaller more hydrophobic one (mol. wt. 20 kDa) has a strongly basic pi and is named /-chain. Both chains are linked by a disulphide bridge between cysteine residues at highly conserved positions in the a(amino acid residue 87) and /-chain (amino acid residue 7). The amino acid sequences ...
... whereas the smaller more hydrophobic one (mol. wt. 20 kDa) has a strongly basic pi and is named /-chain. Both chains are linked by a disulphide bridge between cysteine residues at highly conserved positions in the a(amino acid residue 87) and /-chain (amino acid residue 7). The amino acid sequences ...
Thai Sports Supplements Co., Ltd.
... While whey protein is the best all round protein supplement, there are others that are also important and have advantages in specific areas. Calcium Caseinate is a protein derived from milk and is more effective than whey at preventing muscle tissue breakdown. It enters the blood stream much more sl ...
... While whey protein is the best all round protein supplement, there are others that are also important and have advantages in specific areas. Calcium Caseinate is a protein derived from milk and is more effective than whey at preventing muscle tissue breakdown. It enters the blood stream much more sl ...
Cyclol
![](https://commons.wikimedia.org/wiki/Special:FilePath/Cyclol_reaction.png?width=300)
The cyclol hypothesis is the first structural model of a folded, globular protein. It was developed by Dorothy Wrinch in the late 1930s, and was based on three assumptions. Firstly, the hypothesis assumes that two peptide groups can be crosslinked by a cyclol reaction (Figure 1); these crosslinks are covalent analogs of non-covalent hydrogen bonds between peptide groups. These reactions have been observed in the ergopeptides and other compounds. Secondly, it assumes that, under some conditions, amino acids will naturally make the maximum possible number of cyclol crosslinks, resulting in cyclol molecules (Figure 2) and cyclol fabrics (Figure 3). These cyclol molecules and fabrics have never been observed. Finally, the hypothesis assumes that globular proteins have a tertiary structure corresponding to Platonic solids and semiregular polyhedra formed of cyclol fabrics with no free edges. Such ""closed cyclol"" molecules have not been observed either.Although later data demonstrated that this original model for the structure of globular proteins needed to be amended, several elements of the cyclol model were verified, such as the cyclol reaction itself and the hypothesis that hydrophobic interactions are chiefly responsible for protein folding. The cyclol hypothesis stimulated many scientists to research questions in protein structure and chemistry, and was a precursor of the more accurate models hypothesized for the DNA double helix and protein secondary structure. The proposal and testing of the cyclol model also provides an excellent illustration of empirical falsifiability acting as part of the scientific method.