Molecular Microbiology
... observable growth defect on soybean cultivar OAC Bayfield, a significant growth advantage is conferred by HopZ1b to the growth of a non-pathogenic P. syringae strain on soybean (Zhou et al., 2009). Functional redundancy is very common for T3SEs in P. syringae (Alfano and Collmer, 2004; Kvitko et al. ...
... observable growth defect on soybean cultivar OAC Bayfield, a significant growth advantage is conferred by HopZ1b to the growth of a non-pathogenic P. syringae strain on soybean (Zhou et al., 2009). Functional redundancy is very common for T3SEs in P. syringae (Alfano and Collmer, 2004; Kvitko et al. ...
Chapter 10
... membrane-bound ribosomes (cotranslational translocation) or after their translation has been completed on free ribosomes in the cytosol (posttranslational translocation). In mammalian cells, most proteins enter the ER cotranslationally, whereas both cotranslational and posttranslational pathways are ...
... membrane-bound ribosomes (cotranslational translocation) or after their translation has been completed on free ribosomes in the cytosol (posttranslational translocation). In mammalian cells, most proteins enter the ER cotranslationally, whereas both cotranslational and posttranslational pathways are ...
Reivew, Hemoglobin
... Transport and Removal of CO2 Blood transports two forms of CO2 to the lungs: carbamino-hemoglobin and H2CO3/HCO3- (carbonic acidconjugate base pair) 1. Carbamino-hemoglobin: exposure to low pCO2 results in the reversal of the carbamination reaction by mass action and O2 binding is again favored. ...
... Transport and Removal of CO2 Blood transports two forms of CO2 to the lungs: carbamino-hemoglobin and H2CO3/HCO3- (carbonic acidconjugate base pair) 1. Carbamino-hemoglobin: exposure to low pCO2 results in the reversal of the carbamination reaction by mass action and O2 binding is again favored. ...
Peer-reviewed Article PDF
... form subunits independently from A-band subunits, with the two subsequently stitched together by titin filaments to form a myofibril; (3) premyofibrils and nascent myofibrils as precursors of mature myofibrils; and (4) assembly occurring without any intermediary structures. The premyofibril model, p ...
... form subunits independently from A-band subunits, with the two subsequently stitched together by titin filaments to form a myofibril; (3) premyofibrils and nascent myofibrils as precursors of mature myofibrils; and (4) assembly occurring without any intermediary structures. The premyofibril model, p ...
Communication - Department of Molecular Physiology and Biophysics
... DAP and dystrophin) (1-6). The α-dystroglycan (156-DAG) binds laminin and dystrophin binds to actin filaments, indicating that one function of the dystrophin-glycoprotein complex is to provide a link between the extracellular matrix and the actin cytoskeleton (5, 6). 59-DAP (now named syntrophin) is ...
... DAP and dystrophin) (1-6). The α-dystroglycan (156-DAG) binds laminin and dystrophin binds to actin filaments, indicating that one function of the dystrophin-glycoprotein complex is to provide a link between the extracellular matrix and the actin cytoskeleton (5, 6). 59-DAP (now named syntrophin) is ...
Silaffins of Diatoms: From Applied Biotechnology to Biomedicine
... devices, for example, antibodies can be included in the hologram for optical identification of specific antigens. Butyrylcholinesterase and certain other enzymes (data not provided) maintain their activity when immobilized during in vitro silicon dioxide bioprecipitation [32]. Approximately 90% (± 7 ...
... devices, for example, antibodies can be included in the hologram for optical identification of specific antigens. Butyrylcholinesterase and certain other enzymes (data not provided) maintain their activity when immobilized during in vitro silicon dioxide bioprecipitation [32]. Approximately 90% (± 7 ...
Silaffins of Diatoms: From Applied Biotechnology to
... devices, for example, antibodies can be included in the hologram for optical identification of specific antigens. Butyrylcholinesterase and certain other enzymes (data not provided) maintain their activity when immobilized during in vitro silicon dioxide bioprecipitation [32]. Approximately 90% (± 7 ...
... devices, for example, antibodies can be included in the hologram for optical identification of specific antigens. Butyrylcholinesterase and certain other enzymes (data not provided) maintain their activity when immobilized during in vitro silicon dioxide bioprecipitation [32]. Approximately 90% (± 7 ...
Genome-wide Screen for Inner Nuclear Membrane
... fragment spanned by 2 Spe1 sites into pGEMT (Promega) using Taq (Promega). The plasmids were utilized as templates for reverse PCR amplification and site-directed mutagenesis described below and then the mutant regions were exchanged with the corresponding fragment of pRS415TRM1-II-GFP. pRS415-TRM1 ...
... fragment spanned by 2 Spe1 sites into pGEMT (Promega) using Taq (Promega). The plasmids were utilized as templates for reverse PCR amplification and site-directed mutagenesis described below and then the mutant regions were exchanged with the corresponding fragment of pRS415TRM1-II-GFP. pRS415-TRM1 ...
Repositório da Universidade de Lisboa
... the components are present or absent. Rab proteins are central regulators of this system. One of the objectives of this work is the identification and classification of this protein family in a vast number of organisms that cover, as far as possible, the diversity in Eukaryotes and to make these res ...
... the components are present or absent. Rab proteins are central regulators of this system. One of the objectives of this work is the identification and classification of this protein family in a vast number of organisms that cover, as far as possible, the diversity in Eukaryotes and to make these res ...
PDF
... For this study, we used the consensus site, R⫺3-R⫺2-x⫺1-S兾TB⫹1, that had been defined by previous work with PKA enzymes from a variety of sources, including yeast and humans (19–22). A search of the S. cerevisiae proteome found 553 occurrences of this consensus sequence in 491 proteins (Table 2, whi ...
... For this study, we used the consensus site, R⫺3-R⫺2-x⫺1-S兾TB⫹1, that had been defined by previous work with PKA enzymes from a variety of sources, including yeast and humans (19–22). A search of the S. cerevisiae proteome found 553 occurrences of this consensus sequence in 491 proteins (Table 2, whi ...
Preview Sample 2
... Copyright © 2015 McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education. ...
... Copyright © 2015 McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education. ...
Powerpoint
... Formation of disulfide-S-oxide and subsequent sulfur-sulfur exchange. These RSS can be formed by reaction with various ROS and RNS (e.g. peroxides, peroxynitrite). The oxidation of one sulfur in the disulfide makes the bond more labile and enhances the reaction with a reduced thiol. ...
... Formation of disulfide-S-oxide and subsequent sulfur-sulfur exchange. These RSS can be formed by reaction with various ROS and RNS (e.g. peroxides, peroxynitrite). The oxidation of one sulfur in the disulfide makes the bond more labile and enhances the reaction with a reduced thiol. ...
Storage globulins pass through the Golgi apparatus and
... storage vacuoles (PSVs). Multiple mechanisms have been proposed to be responsible for transporting storage proteins to PSVs in developing seeds. In this study, a specific antibody was raised against the mung bean (Vigna radiata) seed storage protein 8S globulin and its deposition was followed via im ...
... storage vacuoles (PSVs). Multiple mechanisms have been proposed to be responsible for transporting storage proteins to PSVs in developing seeds. In this study, a specific antibody was raised against the mung bean (Vigna radiata) seed storage protein 8S globulin and its deposition was followed via im ...
Photoactivation mechanism of PAmCherry based on crystal
... with new spectral properties, it is essential to gain understanding of the underlying mechanisms of photoactivation. X-ray structures have been reported for a number of irreversible PAFPs, including those that change their fluorescence from green to red upon irradiation with violet light such as Eos ...
... with new spectral properties, it is essential to gain understanding of the underlying mechanisms of photoactivation. X-ray structures have been reported for a number of irreversible PAFPs, including those that change their fluorescence from green to red upon irradiation with violet light such as Eos ...
Anal. Chem. (2011) - American Chemical Society
... approach was performed on peptides27 and proteins28 online with ESI-MS detection. Efficient electrochemical oxidative cleavage of peptides at the C-terminus of tyrosine residues was performed at potentials between 0.5 and 1.5 V (vs a quasi palladium (Pd) reference electrode).27 A recent detailed stu ...
... approach was performed on peptides27 and proteins28 online with ESI-MS detection. Efficient electrochemical oxidative cleavage of peptides at the C-terminus of tyrosine residues was performed at potentials between 0.5 and 1.5 V (vs a quasi palladium (Pd) reference electrode).27 A recent detailed stu ...
Diverse Effects of Mutations in the Signal Sequence on the Secretion of b-lactamase in Salmonella typhimurium.
... form that is trypsin-accessible in intact spheroplasts; the precursors synthesized by the remaining mutants resemble wild-type in that they remain trypsin-inaccessible. One of the latter mutants does produce mature protein, but at a very reduced rate. It thus appears that signalsequence mutations ca ...
... form that is trypsin-accessible in intact spheroplasts; the precursors synthesized by the remaining mutants resemble wild-type in that they remain trypsin-inaccessible. One of the latter mutants does produce mature protein, but at a very reduced rate. It thus appears that signalsequence mutations ca ...
Recent Advances in Target Characterization and Identification by
... γ-Secretase is an integral membrane protease that cleaves the amyloid precursor proteins (APP) to release Aβ peptides, which have a causative role in the pathogenesis of Alzheimer’s disease (AD) [19,20]. γ-Secretase is a complex of four different integral membrane proteins (presenilin, nicastrin, Ap ...
... γ-Secretase is an integral membrane protease that cleaves the amyloid precursor proteins (APP) to release Aβ peptides, which have a causative role in the pathogenesis of Alzheimer’s disease (AD) [19,20]. γ-Secretase is a complex of four different integral membrane proteins (presenilin, nicastrin, Ap ...
Data-driven docking for the study of biomolecular complexes
... With the presently available amount of genetic information, a lot of attention focuses on systems biology and in particular on biomolecular interactions. Considering the huge number of such interactions, and their often weak and transient nature, conventional experimental methods such as Xray crysta ...
... With the presently available amount of genetic information, a lot of attention focuses on systems biology and in particular on biomolecular interactions. Considering the huge number of such interactions, and their often weak and transient nature, conventional experimental methods such as Xray crysta ...
A Nascent Membrane Protein Is Located Adjacent to ER Membrane
... membrane even though translation continues (Yost et al., 1983; Walter and Lingappa, 1986). Upon completion of protein synthesis, the carboxy-terminal end of the membrane protein may be located on either the cytoplasmic or the lumenal side of the ER membrane, depending upon the orientation of the (fi ...
... membrane even though translation continues (Yost et al., 1983; Walter and Lingappa, 1986). Upon completion of protein synthesis, the carboxy-terminal end of the membrane protein may be located on either the cytoplasmic or the lumenal side of the ER membrane, depending upon the orientation of the (fi ...
A Nascent Membrane Protein Is Located Adjacent to
... membrane even though translation continues (Yost et al., 1983; Walter and Lingappa, 1986). Upon completion of protein synthesis, the carboxy-terminal end of the membrane protein may be located on either the cytoplasmic or the lumenal side of the ER membrane, depending upon the orientation of the (fi ...
... membrane even though translation continues (Yost et al., 1983; Walter and Lingappa, 1986). Upon completion of protein synthesis, the carboxy-terminal end of the membrane protein may be located on either the cytoplasmic or the lumenal side of the ER membrane, depending upon the orientation of the (fi ...
proposal-aug25
... specific examples to experimentally test the mechanisms of function of the identified disordered regions. This proposal represents a new approach to attack a difficult problem in protein biochemistry: the function of intrinsically disordered proteins. II. Background and Motivation As many as 50% of ...
... specific examples to experimentally test the mechanisms of function of the identified disordered regions. This proposal represents a new approach to attack a difficult problem in protein biochemistry: the function of intrinsically disordered proteins. II. Background and Motivation As many as 50% of ...
Regulation of Photochemical Energy Transfer Accompanied
... unstacked, while the R25 grana region was more loosely stacked than the L25 grana region (Figure 1d). These results suggest that L40 is a condition in which more LHCII is associated with PSI among these three samples. Starch granules were observed in L25; however, very few starch granules were obser ...
... unstacked, while the R25 grana region was more loosely stacked than the L25 grana region (Figure 1d). These results suggest that L40 is a condition in which more LHCII is associated with PSI among these three samples. Starch granules were observed in L25; however, very few starch granules were obser ...
Protein expression by a Beijing strain differs from that of another
... was mainly growth phase dependent, although some differences between the strains were observed. However, due to the low resolving power of 1D gel electrophoresis, the identity of the differentially expressed proteins could not be ascertained. Here, we have extended our previous study through the use ...
... was mainly growth phase dependent, although some differences between the strains were observed. However, due to the low resolving power of 1D gel electrophoresis, the identity of the differentially expressed proteins could not be ascertained. Here, we have extended our previous study through the use ...
Full-Text PDF
... results were interpreted in the context of the docking results. The results suggest that an extended surface along the active site cleft is involved in binding of a protein substrate. Furthermore, it appears that a number of hydrophobic and aromatic residues are important for interacting with Z-QG, ...
... results were interpreted in the context of the docking results. The results suggest that an extended surface along the active site cleft is involved in binding of a protein substrate. Furthermore, it appears that a number of hydrophobic and aromatic residues are important for interacting with Z-QG, ...
Cyclol
The cyclol hypothesis is the first structural model of a folded, globular protein. It was developed by Dorothy Wrinch in the late 1930s, and was based on three assumptions. Firstly, the hypothesis assumes that two peptide groups can be crosslinked by a cyclol reaction (Figure 1); these crosslinks are covalent analogs of non-covalent hydrogen bonds between peptide groups. These reactions have been observed in the ergopeptides and other compounds. Secondly, it assumes that, under some conditions, amino acids will naturally make the maximum possible number of cyclol crosslinks, resulting in cyclol molecules (Figure 2) and cyclol fabrics (Figure 3). These cyclol molecules and fabrics have never been observed. Finally, the hypothesis assumes that globular proteins have a tertiary structure corresponding to Platonic solids and semiregular polyhedra formed of cyclol fabrics with no free edges. Such ""closed cyclol"" molecules have not been observed either.Although later data demonstrated that this original model for the structure of globular proteins needed to be amended, several elements of the cyclol model were verified, such as the cyclol reaction itself and the hypothesis that hydrophobic interactions are chiefly responsible for protein folding. The cyclol hypothesis stimulated many scientists to research questions in protein structure and chemistry, and was a precursor of the more accurate models hypothesized for the DNA double helix and protein secondary structure. The proposal and testing of the cyclol model also provides an excellent illustration of empirical falsifiability acting as part of the scientific method.