![Dual Location of the Mitochondrial Preprotein](http://s1.studyres.com/store/data/015097372_1-29e664ab37e7e3f8e9621de50f24e46e-300x300.png)
Dual Location of the Mitochondrial Preprotein
... complex (Klodmann et al., 2011; Rode et al., 2011). A comparison of B14.7, Tim23-2, and yeast Tim23 shows that B14.7 is similar to yeast Tim23 in terms of overall characteristics and contains an Arg residue in the correct position of the PRAT consensus domain, G/AX2F/YX10RX3DX6G/A/SGX3G, compared wi ...
... complex (Klodmann et al., 2011; Rode et al., 2011). A comparison of B14.7, Tim23-2, and yeast Tim23 shows that B14.7 is similar to yeast Tim23 in terms of overall characteristics and contains an Arg residue in the correct position of the PRAT consensus domain, G/AX2F/YX10RX3DX6G/A/SGX3G, compared wi ...
Interactions of liposomes and lipid-based carrier systems with blood
... properties and the clearance behavior of liposomes. Our studies to date indicate that there is an inverse relationship between PB values and the circulation half-lives of liposomes. In particular, liposomes that have greater than 50 g protein / mol lipid associated with their membranes are cleared v ...
... properties and the clearance behavior of liposomes. Our studies to date indicate that there is an inverse relationship between PB values and the circulation half-lives of liposomes. In particular, liposomes that have greater than 50 g protein / mol lipid associated with their membranes are cleared v ...
Systematic Structure-Function Analysis of the Small GTPase Arf1 in Yeast.
... Arf proteins are highly conserved and ubiquitous proteins in eukaryotes. Human and yeast Arf1 proteins are 78% identical, and the human ARF1 gene can complement a yeast arf1 mutation (Kahn et al., 1991). Thus, studies of the function of Arf1 in yeast should provide insights that can be generally app ...
... Arf proteins are highly conserved and ubiquitous proteins in eukaryotes. Human and yeast Arf1 proteins are 78% identical, and the human ARF1 gene can complement a yeast arf1 mutation (Kahn et al., 1991). Thus, studies of the function of Arf1 in yeast should provide insights that can be generally app ...
Cambridge Isotope Laboratories, Inc. RefeRences 1. Shadforth, I.P.
... utilizes proteins labeled with heavy stable isotopes, e.g. 15N, 18O, or 13C. Labeled or “heavy” peptides maintain the same chemical characteristics as unlabeled or “light” peptides and c o-elute into the mass spectrometer from liquid chromatography columns. In the mass spectrometer they are easily ...
... utilizes proteins labeled with heavy stable isotopes, e.g. 15N, 18O, or 13C. Labeled or “heavy” peptides maintain the same chemical characteristics as unlabeled or “light” peptides and c o-elute into the mass spectrometer from liquid chromatography columns. In the mass spectrometer they are easily ...
Tissue Regeneration Factor
... of articular cartilage, which covers the surface of a joint where the ends of bones meet. When this cartilage becomes damaged, the bones will grind against each other instead of having a smooth, fluid movement. Since there is no direct blood supply to cartilage, damage to these areas from injuries, ...
... of articular cartilage, which covers the surface of a joint where the ends of bones meet. When this cartilage becomes damaged, the bones will grind against each other instead of having a smooth, fluid movement. Since there is no direct blood supply to cartilage, damage to these areas from injuries, ...
Protein structure is conceptually divided into four
... sequence) is connected to strand n + 3 (a) or n - 3 (b) instead of n + 1 or n - 1 in an eight-stranded antiparallel b sheet or barrel. The two different possible connections give two different hands of the Greek key motif. In all protein structures known so far, the hand shown in (a) has been mostly ...
... sequence) is connected to strand n + 3 (a) or n - 3 (b) instead of n + 1 or n - 1 in an eight-stranded antiparallel b sheet or barrel. The two different possible connections give two different hands of the Greek key motif. In all protein structures known so far, the hand shown in (a) has been mostly ...
Lecture#5 File
... sequence) is connected to strand n + 3 (a) or n - 3 (b) instead of n + 1 or n - 1 in an eight-stranded antiparallel b sheet or barrel. The two different possible connections give two different hands of the Greek key motif. In all protein structures known so far, the hand shown in (a) has been mostly ...
... sequence) is connected to strand n + 3 (a) or n - 3 (b) instead of n + 1 or n - 1 in an eight-stranded antiparallel b sheet or barrel. The two different possible connections give two different hands of the Greek key motif. In all protein structures known so far, the hand shown in (a) has been mostly ...
Effect of Amino Acid Sequence and pH on Nanofiber
... To understand the mechanisms of protein/peptide selfassembly, we chose a model peptide that is simple but possesses all three physicochemical interactions. This peptide EAK16 is known to form unusually stable β-sheet structure.14-17 This peptide has alternating hydrophobic (alanine (A)) and hydrophi ...
... To understand the mechanisms of protein/peptide selfassembly, we chose a model peptide that is simple but possesses all three physicochemical interactions. This peptide EAK16 is known to form unusually stable β-sheet structure.14-17 This peptide has alternating hydrophobic (alanine (A)) and hydrophi ...
Protein sorting at the ER–Golgi interface
... revealing the basis for cargo specificity in COPII vesicle formation (Miller et al., 2003; Mossessova et al., 2003; Mancias and Goldberg, 2007, 2008; Pagant et al., 2015). Yeast Sec24 has at least four distinct cargo-binding sites that each recognize discrete sorting signals, thereby diversifying t ...
... revealing the basis for cargo specificity in COPII vesicle formation (Miller et al., 2003; Mossessova et al., 2003; Mancias and Goldberg, 2007, 2008; Pagant et al., 2015). Yeast Sec24 has at least four distinct cargo-binding sites that each recognize discrete sorting signals, thereby diversifying t ...
ref. #27 of the TIBS article
... Pro had little effect on MGDC, whereas the Leu2 ! Pro substitution led to a substantial decrease in MGDC from 9.7 to 8.5 residues. The effect on MGDC persisted six residues into the hydrophobic segment with a minimum value of MGDC 7.2 for the Leu5 ! Pro substitution, but then quickly disappeared a ...
... Pro had little effect on MGDC, whereas the Leu2 ! Pro substitution led to a substantial decrease in MGDC from 9.7 to 8.5 residues. The effect on MGDC persisted six residues into the hydrophobic segment with a minimum value of MGDC 7.2 for the Leu5 ! Pro substitution, but then quickly disappeared a ...
as PDF
... via the biotin moiety present at the incorporated label. Peptides are then eluted from the column and analyzed by mass spectrometry. Since only cysteine-containing peptides are isolated, the peptide mixture complexity is in general limited, which in principle would enable identification of lower abu ...
... via the biotin moiety present at the incorporated label. Peptides are then eluted from the column and analyzed by mass spectrometry. Since only cysteine-containing peptides are isolated, the peptide mixture complexity is in general limited, which in principle would enable identification of lower abu ...
Specific amino acids in the BAR domain allow homodimerization
... with other BAR domain-containing proteins, in particular with its homologues SNX9 and SNX18, but also with the more distantly related SNX1. As a positive control, SNX33 was used. All four proteins were transiently expressed as HA-tagged proteins in HEK-293/FLAG–SNX33 cells and probed for co-immunopr ...
... with other BAR domain-containing proteins, in particular with its homologues SNX9 and SNX18, but also with the more distantly related SNX1. As a positive control, SNX33 was used. All four proteins were transiently expressed as HA-tagged proteins in HEK-293/FLAG–SNX33 cells and probed for co-immunopr ...
Autophagy in Plasmodium, a multifunctional pathway?
... Adelaide U.P. Hain a, Jürgen Bosch a,* Abstract: Autophagy is a catabolic process that normally utilizes the lysosome. The far-reaching implications of this system in disease are being increasingly understood. Studying autophagy is complicated by its role in cell survival and programmed cell death a ...
... Adelaide U.P. Hain a, Jürgen Bosch a,* Abstract: Autophagy is a catabolic process that normally utilizes the lysosome. The far-reaching implications of this system in disease are being increasingly understood. Studying autophagy is complicated by its role in cell survival and programmed cell death a ...
The Logical Structure of Organic Chemistry and the Empirical
... instead assimilated through ingenious interpretations of abstract mathematical functions. That is to say, while quantum mechanics suggests that electrons in molecules are not localized between adjacent atoms, but extend over the entire space, the valence bond theory takes each covalent bond as a two ...
... instead assimilated through ingenious interpretations of abstract mathematical functions. That is to say, while quantum mechanics suggests that electrons in molecules are not localized between adjacent atoms, but extend over the entire space, the valence bond theory takes each covalent bond as a two ...
- Scholarworks @ CSU San Marcos
... The next step was to use the bmfeat.py program that I had written in python to get three sets of features. The program used the package ProtParam to parse the FASTA files that were outputted from CD-HIT [5] into separate sequences, and then, it allowed us to parse the sequences themselves into indiv ...
... The next step was to use the bmfeat.py program that I had written in python to get three sets of features. The program used the package ProtParam to parse the FASTA files that were outputted from CD-HIT [5] into separate sequences, and then, it allowed us to parse the sequences themselves into indiv ...
The Behavior of Proteins: Enzymes, Mechanisms
... This workforce solution was funded by a grant awarded under the President’s Community-Based Job Training Grants as implemented by the U.S. Department of Labor’s Employment and Training Administration. The solution was created by the grantee and does not necessarily reflect the official position of t ...
... This workforce solution was funded by a grant awarded under the President’s Community-Based Job Training Grants as implemented by the U.S. Department of Labor’s Employment and Training Administration. The solution was created by the grantee and does not necessarily reflect the official position of t ...
James W. Whittaker - Oxygen reactions of the copper oxidases
... oxidases that are the focus of this review, perform a two-electron reduction of O2, forming hydrogen peroxide as the product. Two-electron oxidases serve a variety of biological functions. As peroxidegenerating systems, they may be coupled to peroxidases (haem enzymes requiring hydrogen peroxide as ...
... oxidases that are the focus of this review, perform a two-electron reduction of O2, forming hydrogen peroxide as the product. Two-electron oxidases serve a variety of biological functions. As peroxidegenerating systems, they may be coupled to peroxidases (haem enzymes requiring hydrogen peroxide as ...
Tracing the Archaeal Origins of Eukaryotic Membrane
... evolved from a smaller set of primordial vesicle formation and fusion proteins that were present in early stages of eukaryogenesis. More recent phylogenetic studies have even yielded some insights into the proximal order of events immediately leading to the complexity seen in LECA (Elias et al. 2012 ...
... evolved from a smaller set of primordial vesicle formation and fusion proteins that were present in early stages of eukaryogenesis. More recent phylogenetic studies have even yielded some insights into the proximal order of events immediately leading to the complexity seen in LECA (Elias et al. 2012 ...
Chapter 1: Biological Introduction: RING domain proteins
... the ubiquitination of the substrate. At least three different classes of E3 ligases have been found that mediate substrate ubiquitination. These E3 enzymes differ in the domain that recognizes the E2 enzymes, which can be a RING, a PHD (plant homeodomain) or a HECT (Homologous to E6AP COOH terminus) ...
... the ubiquitination of the substrate. At least three different classes of E3 ligases have been found that mediate substrate ubiquitination. These E3 enzymes differ in the domain that recognizes the E2 enzymes, which can be a RING, a PHD (plant homeodomain) or a HECT (Homologous to E6AP COOH terminus) ...
View PDF - Faculty Web Sites at the University of Virginia
... Although kinesin heavy chains and their homologues have attracted a lion’s share of attention, the light chains have not been completely ignored. Recently, three distinct light chain cDNAs were cloned from rat brain and were completely sequenced [ 19**]. Assuming that translation begins at the first ...
... Although kinesin heavy chains and their homologues have attracted a lion’s share of attention, the light chains have not been completely ignored. Recently, three distinct light chain cDNAs were cloned from rat brain and were completely sequenced [ 19**]. Assuming that translation begins at the first ...
9/12
... Lophotrichous = tuft at one or both ends Peritrichous = spread evenly over the entire surface Arrangement can be useful in identification ...
... Lophotrichous = tuft at one or both ends Peritrichous = spread evenly over the entire surface Arrangement can be useful in identification ...
Review of Osmotic Pressure Driven Release of Proteins from
... An increase in volume fraction of the particles produces a faster release, and as the particle size decreases, the release rate decreases. For slab geometries, the release rate is zero order for much of the release duration; at least until the final layer of particles has swollen and generated micro ...
... An increase in volume fraction of the particles produces a faster release, and as the particle size decreases, the release rate decreases. For slab geometries, the release rate is zero order for much of the release duration; at least until the final layer of particles has swollen and generated micro ...
PDF
... been shown to differ significantly (Costa et al. 2001). On the other hand, the adoption of profitmaximization techniques later in the 1990s has taken into consideration the productivity aspects of economically efficient broiler production. Few models have been developed thus far, and they differ in ...
... been shown to differ significantly (Costa et al. 2001). On the other hand, the adoption of profitmaximization techniques later in the 1990s has taken into consideration the productivity aspects of economically efficient broiler production. Few models have been developed thus far, and they differ in ...
Gene Section NOL3 (nucleolar protein 3 (apoptosis repressor with CARD domain))
... thick boxes) and 3 small introns. The translational start site is in exon 2. Alternative splicing occurs between exons 2 and 3. This involves two splice donors separated by 10 nucleotides in exon 2 connecting to a single splice acceptor in exon 3 (Stoss et al., 1999). Because the separation between ...
... thick boxes) and 3 small introns. The translational start site is in exon 2. Alternative splicing occurs between exons 2 and 3. This involves two splice donors separated by 10 nucleotides in exon 2 connecting to a single splice acceptor in exon 3 (Stoss et al., 1999). Because the separation between ...
Cyclol
![](https://commons.wikimedia.org/wiki/Special:FilePath/Cyclol_reaction.png?width=300)
The cyclol hypothesis is the first structural model of a folded, globular protein. It was developed by Dorothy Wrinch in the late 1930s, and was based on three assumptions. Firstly, the hypothesis assumes that two peptide groups can be crosslinked by a cyclol reaction (Figure 1); these crosslinks are covalent analogs of non-covalent hydrogen bonds between peptide groups. These reactions have been observed in the ergopeptides and other compounds. Secondly, it assumes that, under some conditions, amino acids will naturally make the maximum possible number of cyclol crosslinks, resulting in cyclol molecules (Figure 2) and cyclol fabrics (Figure 3). These cyclol molecules and fabrics have never been observed. Finally, the hypothesis assumes that globular proteins have a tertiary structure corresponding to Platonic solids and semiregular polyhedra formed of cyclol fabrics with no free edges. Such ""closed cyclol"" molecules have not been observed either.Although later data demonstrated that this original model for the structure of globular proteins needed to be amended, several elements of the cyclol model were verified, such as the cyclol reaction itself and the hypothesis that hydrophobic interactions are chiefly responsible for protein folding. The cyclol hypothesis stimulated many scientists to research questions in protein structure and chemistry, and was a precursor of the more accurate models hypothesized for the DNA double helix and protein secondary structure. The proposal and testing of the cyclol model also provides an excellent illustration of empirical falsifiability acting as part of the scientific method.