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DEPARTMENT OF BIOCHEMISTRY UNIVERSITY OF KERALA
DEPARTMENT OF BIOCHEMISTRY UNIVERSITY OF KERALA

thymine dimers - Glen Research
thymine dimers - Glen Research

... the ability to produce oligonucleotides containing leading to incorrect base insertions and subsequent cis-syn thymine dimer at specific locations within mutations.2 the sequence. Unfortunately, the chemical processes The literature covering the chemistry of required to produce cis-syn thymine dimer ...
Development, validation and biological application of
Development, validation and biological application of

... This data raises the question: if PEPCK-C is not important for gluconeogenesis, what is its metabolic role? Previous reports have shown that PEPCK-C is associated with proper maintenance of lipid homeostasis, as animals that are whole body knockouts for this enzyme die within two days of birth, sho ...
Evidence for Evolution
Evidence for Evolution

... 6. At which stage is it the most difficult to tell the embryos apart? (I, II, or III?) 7-9. Describe at least 3 similarities in the structure of these embryos ...
Amino Acids, Proteins, and Enzymes
Amino Acids, Proteins, and Enzymes

... • consists of pyrimidine, p-aminobenzoic acid, and glutamate. • forms the coenzyme THF used in the transfer of carbon groups and the synthesis of nucleic acids. • deficiency can lead to abnormal red blood cells, anemia, and poor growth. ...
Cloning and sequencing of a gene encoding acidophilic amylase
Cloning and sequencing of a gene encoding acidophilic amylase

... Purification of the enzyme and determination of peptide sequences. Plate cultures of B. acidocaldarius were suspended in 0.5 M-Na acetate (PH 50), incubated for 30 min at 4 "C, and the cells were removed by centrifugation at 8000 g for 20 min. The supernatant was centrifuged again at 40000 g for 30 ...
Recombinant Brassinosteroid Insensitive 1 Receptor-Like
Recombinant Brassinosteroid Insensitive 1 Receptor-Like

effect of -fluorination of valproic acid on valproyl- s-acyl
effect of -fluorination of valproic acid on valproyl- s-acyl

... formation of reactive species that bind covalently to important enzymes involved in fatty acid metabolism (Fig. 2). Evidence for the formation of chemically reactive metabolites of VPA and ⌬4-VPA comes from covalent binding studies in isolated rat hepatocytes. Covalent binding was abolished in cells ...
Biology - Kenyon College
Biology - Kenyon College

... For two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (2-D gels), growth media contained LBK broth (10 g of tryptone, 5 g of yeast extract, 7.45 g of KCl) or M63 salts [3 g of KH2PO4, 7 g of K2HPO4, 2 g of (NH4)2SO4, 0.5 ml of FeSO4 at 1 mg/ml, 2 ml of 0.5 M MgSO2, 100 mg of ...
Ca2+ Ions and the Output of Acetylcoenzyme A from Brain
Ca2+ Ions and the Output of Acetylcoenzyme A from Brain

Jasmonate Biosynthesis, Perception and Function in Plant
Jasmonate Biosynthesis, Perception and Function in Plant

... Biosynthesis of JAs originates from polyunsaturated fatty acids (PUFA) and is synthesized by one of the seven distinct branches of the lipoxygenase (LOX) pathway, the allene oxide synthase (AOS) branch (Feussner and Wasternack, 2002). The remaing six branches form other oxylipins including GLVs as w ...
Theoretical studies on pyridoxal 5’-phosphate- catalyzed reactions of biological relevance 2014
Theoretical studies on pyridoxal 5’-phosphate- catalyzed reactions of biological relevance 2014

... for life. Many biological catalysts, also known as enzymes, are proteins which provide a most favorable environment for a s pecific reaction to take place between specific reactants. Some enzymes require the presence of a m etal ion or an organic molecule, known as cofactor, to facilitate the cataly ...
A Complementary Circular Code in the Protein Coding
A Complementary Circular Code in the Protein Coding

... (formed by a series of trinucleotides of X0 ) without using a start codon; (ii) the DNA complementarity property C (e.g. C(AAC) = GTT): C(T0 ) = T0 , C(T1 ) = T2 and C(T2 ) = T1 allowing the two paired reading frames of a DNA double helix simultaneously to code for amino acids; (iii) the circular pe ...
Chem 32 Solutions to Section 15.4 – 15.6 Homework Problems
Chem 32 Solutions to Section 15.4 – 15.6 Homework Problems

... 15.78 Only pathway “c” produces energy that can be harnessed to make ATP. Pathway “a” does not produce or consume a significant amount of energy. Pathway “b” is an activation step, and consumes energy (the body breaks down ATP in this step). 15.86 The starting materials of the citric acid cycle are ...
추가8
추가8

Marine Biotecnology
Marine Biotecnology

... Abstract Penaeus vannamei lipase was purified from midgut gland of whiteleg shrimp. Pure lipase (E.C. 3.1.1.3) was obtained after Superdex 200 gel filtration and Resource Q anionic exchange. The pure lipase, which is a glycosylated molecule, is a monomer having a molecular mass of about 44.8 kDa, as ...
Growth-Environment Dependent Modulation of
Growth-Environment Dependent Modulation of

Purification and Biochemical Characterization of Digestive Lipase in
Purification and Biochemical Characterization of Digestive Lipase in

... Abstract Penaeus vannamei lipase was purified from midgut gland of whiteleg shrimp. Pure lipase (E.C. 3.1.1.3) was obtained after Superdex 200 gel filtration and Resource Q anionic exchange. The pure lipase, which is a glycosylated molecule, is a monomer having a molecular mass of about 44.8 kDa, as ...
Role of Arbuscular Mycorrhizal Fungi in the Nitrogen Uptake of
Role of Arbuscular Mycorrhizal Fungi in the Nitrogen Uptake of

... discuss the regulation of these pathways and major research gaps. Keywords: ammonium; arginine; interface; mycorrhiza; nitrate; nutrient transport; organic nitrogen; periarbuscular membrane; phosphate; plant microbe interaction ...
Luminaries - Oxford Academic
Luminaries - Oxford Academic

... Krebs observed a rapid oxidation of citrate, but interestingly, he identified that citrate was never fully consumed as a substrate, suggesting a capacity for citrate synthesis in this system (Figure 1). In addition, hypoxic conditions (low oxygen) resulted in large amounts of citrate formation in mi ...
Novel evolutionary lineages of the invertebrate oxytocin/vasopressin
Novel evolutionary lineages of the invertebrate oxytocin/vasopressin

... The total amino acid sequence of the CTR2 is 33.1–48.1 % homologous to the sequences of other OT/VP receptor family members, and OPR is 24.9–40.5 % homologous (Table 2). The sequence of similarity of CTR2 and OPR to the OT receptor family is not so different from their similarity to VP receptors. Wh ...
Crystal structure of potato tuber ADP‐glucose pyrophosphorylase
Crystal structure of potato tuber ADP‐glucose pyrophosphorylase

... synthetic eukaryotes and bacteria, and UDP-glucose (UDPGlc) in mammals, fungi, and eukaryotic heterotrophic microorganisms (Sivak and Preiss, 1998). In plants and bacteria, the main regulatory step of the pathway is ADP-Glc synthesis catalyzed by ADP-glucose pyrophosphorylase (EC 2.7.7.27; ADP-Glc P ...
Wax ester fermentation and fatty acid biosynthesis in the facultatively
Wax ester fermentation and fatty acid biosynthesis in the facultatively

... nutrients such as glucose, undergo a series of transfers along the respiratory enzyme complexes. The electrons release energy by each transfer among the transport chain towards oxygen which serves as terminal electron acceptor. The energy released by the transfer among the transport chain, localized ...
(PSD) July 2016 PBAC Meeting
(PSD) July 2016 PBAC Meeting

... Shake & Go® does not contain choline, whereas the comparator, TYR Express 15®, does contain choline. The NPWP noted that there is a Recommended Daily Intake (RDI) level for choline, and patients on a TYRsuitable diet are likely to have low intake of choline. The NPWP recalled a similar submission fr ...
video slide
video slide

... NADH, forming lactate as an end product, with no release of CO2 Lactic acid fermentation by some fungi and bacteria is used to make cheese and yogurt Human muscle cells use lactic acid fermentation to generate ATP when O2 is scarce ...
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Amino acid synthesis

Amino acid synthesis is the set of biochemical processes (metabolic pathways) by which the various amino acids are produced from other compounds. The substrates for these processes are various compounds in the organism's diet or growth media. Not all organisms are able to synthesise all amino acids. Humans are excellent example of this, since humans can only synthesise 11 of the 20 standard amino acids (aka non-essential amino acid), and in time of accelerated growth, arginine, can be considered an essential amino acid.A fundamental problem for biological systems is to obtain nitrogen in an easily usable form. This problem is solved by certain microorganisms capable of reducing the inert N≡N molecule (nitrogen gas) to two molecules of ammonia in one of the most remarkable reactions in biochemistry. Ammonia is the source of nitrogen for all the amino acids. The carbon backbones come from the glycolytic pathway, the pentose phosphate pathway, or the citric acid cycle.In amino acid production, one encounters an important problem in biosynthesis, namely stereochemical control. Because all amino acids except glycine are chiral, biosynthetic pathways must generate the correct isomer with high fidelity. In each of the 19 pathways for the generation of chiral amino acids, the stereochemistry at the α-carbon atom is established by a transamination reaction that involves pyridoxal phosphate. Almost all the transaminases that catalyze these reactions descend from a common ancestor, illustrating once again that effective solutions to biochemical problems are retained throughout evolution.Biosynthetic pathways are often highly regulated such that building-blocks are synthesized only when supplies are low. Very often, a high concentration of the final product of a pathway inhibits the activity of enzymes that function early in the pathway. Often present are allosteric enzymes capable of sensing and responding to concentrations of regulatory species. These enzymes are similar in functional properties to aspartate transcarbamoylase and its regulators. Feedback and allosteric mechanisms ensure that all twenty amino acids are maintained in sufficient amounts for protein synthesis and other processes.
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