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A E M , Feb. 2004, p. 999–1007
A E M , Feb. 2004, p. 999–1007

... (15) or the Umbellularia californica thioesterase gene and PHA synthase (26) in E. coli grown in the presence of gluconate to accumulate MCL PHA. Coexpression of the E. coli tesA gene and the Pseudomonas oleovorans phaC1 gene led to the accumulation of MCL PHA copolymer composed of C6, C8, and C10 m ...
Methylobacterium extorquens AM1
Methylobacterium extorquens AM1

... metabolic pathways such as the tetrahydromethanopterin-dependent oxidation pathway, the serine cycle, and the ethylmalonyl-CoA pathway are essential during growth on methanol but dispensable for growth on organic acids (although some individual enzymes may still be required). In contrast, a complete ...
Two nearly identical terpene synthases catalyze the
Two nearly identical terpene synthases catalyze the

... snapdragon myrcene synthase (AAO41726), (E)-b-ocimene synthase (AAO42614), strawberry nerolidol/linalool synthase (FaNES; CAD57106) and Arabidopsis linalool synthase (AAO85533). The alignment was performed using CLUSTALW, and was shaded with BoxShade version 3.21 software (Human Genome Sequencing Ce ...
Protein for Athletes
Protein for Athletes

... regulator of protein synthesis and is donor of nitrogen to alanine and glutamine, 2 important amino acids in muscle protein synthesis. Researchers are excited about this ‘‘leucine trigger,’’ as it may have important implications not only for muscle growth for healthy athletes but also for prevention ...
Substrate Specificity of Tonin from Rat Submaxillary Gland
Substrate Specificity of Tonin from Rat Submaxillary Gland

... The almost identical values obtained with tonin for angiotensin I and (des-Asp^angiotensin I demonstrate that these two peptides are equally good substrates, whereas the 10 times lower hydrolysis rate observed with (des-Asp^desArg*)-angiotensin I reveals that the octapeptide is a poor substrate. Ear ...
Regulation of Translation by Essential Amino Acids and Glucose in
Regulation of Translation by Essential Amino Acids and Glucose in

... Amino Acid Nutrition Essential amino acids and N must be absorbed in sufficient quantities from the gastrointestinal tract to support the synthesis of milk protein and the maintenance of body tissues in the lactating cow. Dietary protein can be classified as either rumen-degradable (RDP) or rumen-un ...
Cloning and sequence analysis of cnaA gene encoding the catalytic
Cloning and sequence analysis of cnaA gene encoding the catalytic

... to study its function in detail. While earlier reports of calcineurin A from ¢lamentous fungi suggested its requirement for hyphal growth and cell cycle regulation [5,6], a putative role of this protein phosphatase in sporulation, salt stress response and the alkaline pH-mediated signal transduction ...
Ana Maria da Silva Esteves Dissertation presented to obtain
Ana Maria da Silva Esteves Dissertation presented to obtain

Neighbor-Dependent Ramachandran Probability Distributions of
Neighbor-Dependent Ramachandran Probability Distributions of

PDF
PDF

... Keywords: metabolic energy; insulin; glucagon; glucose; synthesis; degradation 1. Introduction and background The stimulation of metabolic enzymes partially relies on hormonal balance. Thus, a comprehension of the roles of hormones in metabolic regulation is essential to evaluate of any metabolic ac ...
25., Fatty ocid oxidation
25., Fatty ocid oxidation

... In Chapter 24 we saw that the carbons of the aceryl CoA produced by the catabolism of glucose can be completely oxidized to carbon dioxide in the citric acid cycle. Each molecule of acetyl CoA oxidized in this fashion yields enough energy to make one molecule of AIB one molecule of FADH2,and three m ...
Interactions of TCA cycle enzymes and of the CcpA
Interactions of TCA cycle enzymes and of the CcpA

... Interaktion des Icd-Isocitrat-NADP+-Mg2+-Komplexes mit Mdh zu charakterisieren, wurden kinetische SPR Analysen durchgeführt. Da Wildtyp Icd die Substrate für diese Analyse zu schnell umsetzte, wurde stattdessen eine IcdS104P Mutante eingesetzt. Die Interaktion des Enzyms mit Mdh wurde durch die Muta ...
Are You suprised ?
Are You suprised ?

... is determined by the sequence of nucleotides in a gene. A change in the DNA nucleotide sequence (mutation) of a gene that codes for a protein may result in a change in the amino-acid sequence of the protein. Biochemical evidence of evolution compares favorably with structural evidence of evolution. ...
Nitrate regulation of metabolism and growth Mark Stitt
Nitrate regulation of metabolism and growth Mark Stitt

... nitrate and sugars are illustrated by the demonstration that both have to be supplied to achieve high rates of nitrate assimilation and amino acid synthesis in detached leaves [38•]. High sucrose stimulates the conversion of nitrate to glutamine and, especially, the conversion of glycerate-3-phospha ...
GSS: Gonad-Stimulating Substance
GSS: Gonad-Stimulating Substance

... A RELAXIN-LIKE PEPTIDE PURIFIED FROM RADIAL NERVES INDUCES OOCYTE MATURATION AND OVULATION IN THE STARFISH, ASTERINA PECTINIFERA Gonad-stimulating substance (GSS) of starfish is the only known invertebrate peptide hormone responsible for final gamete maturation, rendering it functionally analogous t ...
Characterization of the unique intron
Characterization of the unique intron

... multigene family comprising approximately 3 - 2 0 members depending upon the organism studied (1). LHCPIIs have been classified into three types based on the amino acids found at 14 positions and the presence or absence of introns within their genes (2). Type I LHCPIIs are encoded by intronless gene ...
Novel Allergen Structures with Tandem Amino Acid Repeats
Novel Allergen Structures with Tandem Amino Acid Repeats

... sequences. Close inspection of the sequences revealed segments that were repeated every ;100 amino acids and suggested that the sizes of the Bla g 1, Per a 1, and related cDNAs varied with the number of repeat sequences they contained. The repeats were compared using a dot-plot matrix analysis that ...
Aminoacylated tmRNA from Escherichia coli interacts with
Aminoacylated tmRNA from Escherichia coli interacts with

... panels A and B, T. thermophilus EF-Tu protects the deacylation of alanylated wild-type E. coli tmRNA similarly or even better than for alanylated tRNAAla + The calculated half-life (t1/2 ) of the Ala-tmRNA ester bond is 17 min without EF-Tu-GTP and over 6 h in its presence (Table 1)+ Similar results ...
Identifying producers of antibacterial compounds by
Identifying producers of antibacterial compounds by

... These modifications can affect pharmacology and target affinity and, in the case of antibiotics, mitigate resistance. One of the main challenges in the field is the identification of microbes with the biosynthetic capacity to produce desired compound classes. It has been estimated, for example, that ...
Genetic Disorders Leading to Hypoglycaemia
Genetic Disorders Leading to Hypoglycaemia

... In the face of hypoglycaemia, infants with CHI have inappropriately elevated serum insulin levels, low ketone bodies, and low fatty acids and show a glycaemic response to glucagon. Infants with CHI typically need a glucose infusion rate of more than 8 mg/kg/min to maintain normoglycaemia. In patient ...
course title - Saylor Academy
course title - Saylor Academy

... The reaction mechanism is the step-by-step sequence of events in a chemical reaction. It includes breaking chemical bonds, describing transition state intermediates, and making chemical bonds. In this unit, you will start with an overview of the functional groups of organic molecules. Next, you will ...
Types and effects of protein variations. Vihinen
Types and effects of protein variations. Vihinen

... Variations manifest their effects at different ways. Of the identified disease-causing cases large proportion appears in protein coding DNA and RNA sequences, although the proteincoding regions constitute only about 1.3 % of human genome. Due to the large number of different functions in which prote ...
ICoMST-Abstract_YUFU
ICoMST-Abstract_YUFU

... summarized in Fig. 1. There were overall 782 and 775 potential ACE-inhibitory peptides for released from alpha-1(I) and alpha-2(I) collagen, respectively. Papain (100), chymotrypsin C (91), and ficain (89) were demonstrated to be the most effective enzymes in producing the most ACE inhibitory peptid ...
09_Lectures_PPT
09_Lectures_PPT

... produce ATP without the use of oxygen • Cellular respiration requires O2 to produce ATP • Glycolysis can produce ATP with or without O2 (in aerobic or anaerobic conditions) • In the absence of O2, glycolysis couples with fermentation to produce ATP ...
09_Lectures_PPT
09_Lectures_PPT

... produce ATP without the use of oxygen • Cellular respiration requires O2 to produce ATP • Glycolysis can produce ATP with or without O2 (in aerobic or anaerobic conditions) • In the absence of O2, glycolysis couples with fermentation to produce ATP ...
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Amino acid synthesis

Amino acid synthesis is the set of biochemical processes (metabolic pathways) by which the various amino acids are produced from other compounds. The substrates for these processes are various compounds in the organism's diet or growth media. Not all organisms are able to synthesise all amino acids. Humans are excellent example of this, since humans can only synthesise 11 of the 20 standard amino acids (aka non-essential amino acid), and in time of accelerated growth, arginine, can be considered an essential amino acid.A fundamental problem for biological systems is to obtain nitrogen in an easily usable form. This problem is solved by certain microorganisms capable of reducing the inert N≡N molecule (nitrogen gas) to two molecules of ammonia in one of the most remarkable reactions in biochemistry. Ammonia is the source of nitrogen for all the amino acids. The carbon backbones come from the glycolytic pathway, the pentose phosphate pathway, or the citric acid cycle.In amino acid production, one encounters an important problem in biosynthesis, namely stereochemical control. Because all amino acids except glycine are chiral, biosynthetic pathways must generate the correct isomer with high fidelity. In each of the 19 pathways for the generation of chiral amino acids, the stereochemistry at the α-carbon atom is established by a transamination reaction that involves pyridoxal phosphate. Almost all the transaminases that catalyze these reactions descend from a common ancestor, illustrating once again that effective solutions to biochemical problems are retained throughout evolution.Biosynthetic pathways are often highly regulated such that building-blocks are synthesized only when supplies are low. Very often, a high concentration of the final product of a pathway inhibits the activity of enzymes that function early in the pathway. Often present are allosteric enzymes capable of sensing and responding to concentrations of regulatory species. These enzymes are similar in functional properties to aspartate transcarbamoylase and its regulators. Feedback and allosteric mechanisms ensure that all twenty amino acids are maintained in sufficient amounts for protein synthesis and other processes.
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