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... thymine DNA glycosylase (TDG) releases thymine or uracil from G•T and G•U mismatches and remains stably bound to the resulting abasic site until it is transferred to the next enzyme in the repair pathway. SUMO conjugation to TDG promotes its release from the abasic site, and the 2.1-Å-resolution cry ...
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... Maximum interaction (about 70% degree of interaction) at pH 6.5. At lower pH, there is a slow decline - to about 50% at pH 3.6. At higher pH, rapid decrease of degree of interaction - 13% at pH 8.3. Explanation: Protein-starch interaction requires (+) charge of protein molecules which decline in alk ...
ANATOMY LECTURE EXAM 1
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... e. anterior 5. Body structures known as _____ are composed of two or more different tissues and usually have recognizable shapes. a. cells b. organs c. organ systems d. systems 6. The human body is estimated to have nearly 100 _____ cells with _____ cell types. a. million; about 25 b. billion; about ...
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Cell-penetrating peptide



Cell-penetrating peptides (CPPs) are short peptides that facilitate cellular uptake of various molecular cargo (from nanosize particles to small chemical molecules and large fragments of DNA). The ""cargo"" is associated with the peptides either through chemical linkage via covalent bonds or through non-covalent interactions. The function of the CPPs are to deliver the cargo into cells, a process that commonly occurs through endocytosis with the cargo delivered to the endosomes of living mammalian cells.CPPs hold great potential as in vitro and in vivo delivery vectors for use in research and medicine. Current use is limited by a lack of cell specificity in CPP-mediated cargo delivery and insufficient understanding of the modes of their uptake.CPPs typically have an amino acid composition that either contains a high relative abundance of positively charged amino acids such as lysine or arginine or has sequences that contain an alternating pattern of polar/charged amino acids and non-polar, hydrophobic amino acids. These two types of structures are referred to as polycationic or amphipathic, respectively. A third class of CPPs are the hydrophobic peptides, containing only apolar residues, with low net chargeor have hydrophobic amino acid groups that are crucial for cellular uptake.The first CPP was discovered independently by two laboratories in 1988, when it was found that the trans-activating transcriptional activator (TAT) from human immunodeficiency virus 1 (HIV-1) could be efficiently taken up from the surrounding media by numerous cell types in culture. Since then, the number of known CPPs has expanded considerably and small molecule synthetic analogues with more effective protein transduction properties have been generated.
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