A12_ScienceAndFiction - Collegiate Quiz Bowl Packet Archive
... a. 5: Name the condition in which an individual's red-blood cell has lost its standard shape, sometimes preventing easy movement through capillaries. Answer: sickle-cell anemia (accept Beta-thalassemia) b. 10: Adult hemoglobin is made up of two different types of subunits. Name the protein or gene o ...
... a. 5: Name the condition in which an individual's red-blood cell has lost its standard shape, sometimes preventing easy movement through capillaries. Answer: sickle-cell anemia (accept Beta-thalassemia) b. 10: Adult hemoglobin is made up of two different types of subunits. Name the protein or gene o ...
The enhancement of ribosomal transcription by the recycling of RNA
... then no longer depend solely on random collisions between the promoter or the spacer enhancer elements and the polymerase. Thus, yet a further mechanism to enhance the rate of ribosomal transcription may occur. Recycling of transcription factors could function either through release of the polymeras ...
... then no longer depend solely on random collisions between the promoter or the spacer enhancer elements and the polymerase. Thus, yet a further mechanism to enhance the rate of ribosomal transcription may occur. Recycling of transcription factors could function either through release of the polymeras ...
`Candidatus Phytoplasma mali`, `Candidatus Phytoplasma pyri` and
... coherent, genus-level taxon. In the monophyletic phytoplasma clade, groups and subgroups have been delineated, many of which are being considered as putative species under the provisional status ‘Candidatus’ (Seemüller et al., 2002) for incompletely described prokaryotes, according to Murray & Stac ...
... coherent, genus-level taxon. In the monophyletic phytoplasma clade, groups and subgroups have been delineated, many of which are being considered as putative species under the provisional status ‘Candidatus’ (Seemüller et al., 2002) for incompletely described prokaryotes, according to Murray & Stac ...
The different roles of tryptophan transfer RNA in regulating trp
... compared in this article, Escherichia coli can degrade tryptophan to indole, pyruvate and ammonia, whereas Bacillus subtilis can not. Situations undoubtedly arose in nature where separate events affected the levels of tryptophan and charged tRNATrp, therefore, it was necessary to design strategies t ...
... compared in this article, Escherichia coli can degrade tryptophan to indole, pyruvate and ammonia, whereas Bacillus subtilis can not. Situations undoubtedly arose in nature where separate events affected the levels of tryptophan and charged tRNATrp, therefore, it was necessary to design strategies t ...
Lecture 1 - "Hudel" Luecke
... Proteins are very complicated moleucules. With 20 different amino acids that can be arranged in any order to make a polypeptide of up to thousands of amino acids long, their potential for variety is extraordinary. This variety allows proteins to function as exquisitely specific enzymes that compose ...
... Proteins are very complicated moleucules. With 20 different amino acids that can be arranged in any order to make a polypeptide of up to thousands of amino acids long, their potential for variety is extraordinary. This variety allows proteins to function as exquisitely specific enzymes that compose ...
Mutations at the Darkener of apricot Locus Modulate Transcript
... heteroallelic Doa mutant fea truncated RNA (W. MATTOX,personal communication). males, it is completely suppressed (Figure 2). In males Beadex alleles are hypermorphs, since duplications of the from this cross, the dose of functional Doa product wild-typelocusyield a recessive Beadex phenotype (LIFal ...
... heteroallelic Doa mutant fea truncated RNA (W. MATTOX,personal communication). males, it is completely suppressed (Figure 2). In males Beadex alleles are hypermorphs, since duplications of the from this cross, the dose of functional Doa product wild-typelocusyield a recessive Beadex phenotype (LIFal ...
TaqMan-Based Real-Time PCR for Genotyping Common
... and HP2 for typing (14, 15 ). More recently, a singlechain antibody-based ELISA test was also established (16 ). In addition, several HP-genotyping methods based on conventional PCR have been developed; however, these PCR-based methods need to amplify a relatively large DNA fragment to detect the 1. ...
... and HP2 for typing (14, 15 ). More recently, a singlechain antibody-based ELISA test was also established (16 ). In addition, several HP-genotyping methods based on conventional PCR have been developed; however, these PCR-based methods need to amplify a relatively large DNA fragment to detect the 1. ...
1999 Dekkers: BREEDING IN THE 21st CENTURY
... Instead, BLUP EBV are by definition unbiased: even for the top bulls, their EBV is expected to be equal to their true breeding value. Based on this analogy, it is important that genetic evaluation procedures that use molecular information properly incorporate uncertainty about QTL parameters and est ...
... Instead, BLUP EBV are by definition unbiased: even for the top bulls, their EBV is expected to be equal to their true breeding value. Based on this analogy, it is important that genetic evaluation procedures that use molecular information properly incorporate uncertainty about QTL parameters and est ...
Properties of Mitotic and Meiotic Recombination in the
... array, and heterozygous for markers flanking the array, we measured inter-homolog recombination and intra-/sister-chromatid exchange in the CUP1 locus. The rate of intra-/sister chromatid recombination exceeded the rate of inter-homolog recombination by more than ten-fold. Loss of the Rad51 and Rad5 ...
... array, and heterozygous for markers flanking the array, we measured inter-homolog recombination and intra-/sister-chromatid exchange in the CUP1 locus. The rate of intra-/sister chromatid recombination exceeded the rate of inter-homolog recombination by more than ten-fold. Loss of the Rad51 and Rad5 ...
Product description P003-D1 MLH1-MSH2-v01 - MRC
... the same procedure, and prepared using the same DNA extraction method as the patient samples. Reference samples should be derived from unrelated individuals who are from families without a history of hereditary predisposition to cancer. More information regarding the selection and use of reference s ...
... the same procedure, and prepared using the same DNA extraction method as the patient samples. Reference samples should be derived from unrelated individuals who are from families without a history of hereditary predisposition to cancer. More information regarding the selection and use of reference s ...
Landick R, Yanofsky C. 1987. Transcription
... synthetase (119,180) or alterations in the modifying enzyme that adds the isopentenyl group to the A residue adjacent to the anticodon of tRNATrp (41, 42, 170, 180). The discovery of a ribosome-binding site in vitro (134) and its correspondence to a potential translation initiation site in the leade ...
... synthetase (119,180) or alterations in the modifying enzyme that adds the isopentenyl group to the A residue adjacent to the anticodon of tRNATrp (41, 42, 170, 180). The discovery of a ribosome-binding site in vitro (134) and its correspondence to a potential translation initiation site in the leade ...
Streptococcus faecium - International Journal of Systematic and
... Transfer of Streptococcus faecalis and Streptococcus faecium to the Genus Enterococcus norn. rev. as Enterococcus faecalis comb. nov. and Enterococcus faecium comb. nov. KARL H. SCHLEIFER* A N D RENATE KILPPER-BALZ Lehrstuhl fur Mikrobiologie, Technische Universitat Miinchen, D-8000 Miinchen 2, Fede ...
... Transfer of Streptococcus faecalis and Streptococcus faecium to the Genus Enterococcus norn. rev. as Enterococcus faecalis comb. nov. and Enterococcus faecium comb. nov. KARL H. SCHLEIFER* A N D RENATE KILPPER-BALZ Lehrstuhl fur Mikrobiologie, Technische Universitat Miinchen, D-8000 Miinchen 2, Fede ...
Aminolaevulinic acid synthase of Rhodobacter capsulatus: high
... anaemia [7]. In a Claisen-type condensation reaction, involving the untypical cleavage of two amino acid α-carbon bonds, succinyl-CoA and glycine are converted by ALAS into CO2 , CoA and the general haem precursor ALA (aminolaevulinic acid) [8]. A few years ago we solved the, as of yet, only crystal ...
... anaemia [7]. In a Claisen-type condensation reaction, involving the untypical cleavage of two amino acid α-carbon bonds, succinyl-CoA and glycine are converted by ALAS into CO2 , CoA and the general haem precursor ALA (aminolaevulinic acid) [8]. A few years ago we solved the, as of yet, only crystal ...
tetrahedron report number 124 suicide substrates
... The second generation of enzyme-specific inactivation reagents have been in use for about the past decade and differ from the first in that the reactive functional group is latent in the molecules in solution. Only after binding to the target enzyme and after the enzyme begins catalysis is the react ...
... The second generation of enzyme-specific inactivation reagents have been in use for about the past decade and differ from the first in that the reactive functional group is latent in the molecules in solution. Only after binding to the target enzyme and after the enzyme begins catalysis is the react ...
NGRLW_SPODS_2.2 - National Genetics Reference Laboratories
... primers also have filler bases[s] between the GS sequence and the tag: these are included to prevent the formation of a highly stable hairpin structures. Primers with the US1 tag are designated GS1 and primers with the US2 tag are designated GS2. The final component of the system is a set of univers ...
... primers also have filler bases[s] between the GS sequence and the tag: these are included to prevent the formation of a highly stable hairpin structures. Primers with the US1 tag are designated GS1 and primers with the US2 tag are designated GS2. The final component of the system is a set of univers ...
Designing synthetic MLPA probes - MRC
... http://www.ncbi.nlm.nih.gov/mapview/map_search.cgi?taxid=9606 Sequence between start and stop codon. In Map Viewer, numbers mentioned under CDS refer to numbering of the Genbank entry of NM sequence. Most of our probes are located in coding regions of genes. We use the term Forward Probe when the hy ...
... http://www.ncbi.nlm.nih.gov/mapview/map_search.cgi?taxid=9606 Sequence between start and stop codon. In Map Viewer, numbers mentioned under CDS refer to numbering of the Genbank entry of NM sequence. Most of our probes are located in coding regions of genes. We use the term Forward Probe when the hy ...
Natural selection and the maximization of fitness
... Intuitively, the source of the trouble in multi-locus models is that Mendelian segregation, recombination and epistasis complicate the transmission of fitness between parents and offspring. Offspring, while resembling their parents on the whole, inherit a combination of genes that is not a simple re ...
... Intuitively, the source of the trouble in multi-locus models is that Mendelian segregation, recombination and epistasis complicate the transmission of fitness between parents and offspring. Offspring, while resembling their parents on the whole, inherit a combination of genes that is not a simple re ...
- Boreskov Institute of Catalysis
... More than the 50-year-long history of the Institute is a glowing example of fruitful interaction between fundamental science and industry and of the profound realization that solving scientific problems should be aimed at some practical result. The accomplishments of the Institute embody its origina ...
... More than the 50-year-long history of the Institute is a glowing example of fruitful interaction between fundamental science and industry and of the profound realization that solving scientific problems should be aimed at some practical result. The accomplishments of the Institute embody its origina ...
Simple identification of dominant p53 mutants by
... impaired degradation model provides a satisfactory explanation for the high level p53 expression seen in tumours (5,6), it does not rule out the possibility that a selective growth advantage may be conferred by mutated p53 proteins, either because dominant inhibition of wild-type protein could facil ...
... impaired degradation model provides a satisfactory explanation for the high level p53 expression seen in tumours (5,6), it does not rule out the possibility that a selective growth advantage may be conferred by mutated p53 proteins, either because dominant inhibition of wild-type protein could facil ...
Biochemistry 1997 Baird
... important for acidification of urine (Liljas et al., 1972; Sato et al., 1990; Sly et al., 1983; 1985a,b) while membrane-bound CA IV is located in the apical plasma membranes of the brush border in the proximal convoluted tubule and the thick ascending limb of Henle (Brown et al., 1990). CA IV plays ...
... important for acidification of urine (Liljas et al., 1972; Sato et al., 1990; Sly et al., 1983; 1985a,b) while membrane-bound CA IV is located in the apical plasma membranes of the brush border in the proximal convoluted tubule and the thick ascending limb of Henle (Brown et al., 1990). CA IV plays ...
Deoxyribozyme
Deoxyribozymes, also called DNA enzymes, DNAzymes, or catalytic DNA, are DNA oligonucleotides that are capable of catalyzing specific chemical reactions, similar to the action of other biological enzymes, such as proteins or ribozymes (enzymes composed of RNA).However, in contrast to the abundance of protein enzymes in biological systems and the discovery of biological ribozymes in the 1980s,there are no known naturally occurring deoxyribozymes.Deoxyribozymes should not be confused with DNA aptamers which are oligonucleotides that selectively bind a target ligand, but do not catalyze a subsequent chemical reaction.With the exception of ribozymes, nucleic acid molecules within cells primarily serve as storage of genetic information due to its ability to form complementary base pairs, which allows for high-fidelity copying and transfer of genetic information. In contrast, nucleic acid molecules are more limited in their catalytic ability, in comparison to protein enzymes, to just three types of interactions: hydrogen bonding, pi stacking, and metal-ion coordination. This is due to the limited number of functional groups of the nucleic acid monomers: while proteins are built from up to twenty different amino acids with various functional groups, nucleic acids are built from just four chemically similar nucleobases. In addition, DNA lacks the 2'-hydroxyl group found in RNA which limits the catalytic competency of deoxyribozymes even in comparison to ribozymes.In addition to the inherent inferiority of DNA catalytic activity, the apparent lack of naturally occurring deoxyribozymes may also be due to the primarily double-stranded conformation of DNA in biological systems which would limit its physical flexibility and ability to form tertiary structures, and so would drastically limit the ability of double-stranded DNA to act as a catalyst; though there are a few known instances of biological single-stranded DNA such as multicopy single-stranded DNA (msDNA), certain viral genomes, and the replication fork formed during DNA replication. Further structural differences between DNA and RNA may also play a role in the lack of biological deoxyribozymes, such as the additional methyl group of the DNA base thymidine compared to the RNA base uracil or the tendency of DNA to adopt the B-form helix while RNA tends to adopt the A-form helix. However, it has also been shown that DNA can form structures that RNA cannot, which suggests that, though there are differences in structures that each can form, neither is inherently more or less catalytic due to their possible structural motifs.