Cold-induced silencing by long antisense transcripts of an
... sensor construct, showed by strand-specific RT–PCR analysis (Fig. 4b). Sequencing of the PCR products showed the transcript to be spliced at the same position as COOLAIR (Supplementary Fig. 2d) and extend from the COOLAIR promoter to at least half way through the transgene (Fig. 4a). The cold-induce ...
... sensor construct, showed by strand-specific RT–PCR analysis (Fig. 4b). Sequencing of the PCR products showed the transcript to be spliced at the same position as COOLAIR (Supplementary Fig. 2d) and extend from the COOLAIR promoter to at least half way through the transgene (Fig. 4a). The cold-induce ...
Searching for Compact Hierarchical Structures in DNA by means of
... fruitful clash with the deep questions underlying information science. Brooks identified in 2003 recent development in biology as the necessary pressure to finally develop a long-time needed quantification of structural information [39]. This thesis is motivated by automatically learning structural mod ...
... fruitful clash with the deep questions underlying information science. Brooks identified in 2003 recent development in biology as the necessary pressure to finally develop a long-time needed quantification of structural information [39]. This thesis is motivated by automatically learning structural mod ...
Presence of multiple group I introns closely 23S rRNAs of lichen-forming
... Relationship between introns at positions L1917, L1931 and L1951 with bacterial group I introns. With respect to host evolution, an examination of the LSU rDNA tree (Fig. 1B) shows that the members of the Trebouxia genus analyzed in this study form two distinct main lineages: lineage 1, defined by T ...
... Relationship between introns at positions L1917, L1931 and L1951 with bacterial group I introns. With respect to host evolution, an examination of the LSU rDNA tree (Fig. 1B) shows that the members of the Trebouxia genus analyzed in this study form two distinct main lineages: lineage 1, defined by T ...
Chem Soc Rev
... molecule, and thus, these products may easily undergo consecutive conversion such as deep oxidation to CO and CO2 under the reaction conditions. The activation and selective transformation of alkanes including methane represents a ‘‘Holy Grail’’ in the chemical community.19 Hydrogen as the cleanest ...
... molecule, and thus, these products may easily undergo consecutive conversion such as deep oxidation to CO and CO2 under the reaction conditions. The activation and selective transformation of alkanes including methane represents a ‘‘Holy Grail’’ in the chemical community.19 Hydrogen as the cleanest ...
W. Ford Doolittle - of Maureen A. O`Malley
... that are not classified as natural science, others draw resources from more distant disciplines. Ford Doolittle has maintained throughout his career a deep interest in the humanities and arts, which might be explained to some extent by his father’s academic ...
... that are not classified as natural science, others draw resources from more distant disciplines. Ford Doolittle has maintained throughout his career a deep interest in the humanities and arts, which might be explained to some extent by his father’s academic ...
The Nicotiana tabacum genome encodes two cytoplasmic
... kit from Stratagene, according to the recommendations of the manufacturer. From the partial genomic phage library, 400 O00 plaques of the recombinant bacteriophage were plated at a density of about 50 O00 per plate, transferred onto Hybond N f membrane (Amersham) and screened with the thioredoxin X5 ...
... kit from Stratagene, according to the recommendations of the manufacturer. From the partial genomic phage library, 400 O00 plaques of the recombinant bacteriophage were plated at a density of about 50 O00 per plate, transferred onto Hybond N f membrane (Amersham) and screened with the thioredoxin X5 ...
International Agency for Research on Cancer (IARC)
... Mixtures of aflatoxins and aflatoxin B1 have been tested extensively for carcinogenicity by various routes of administration in several strains of mice and rats, in hamsters, several strains of fish, ducks, tree shrews and monkeys. Following their oral administration, mixtures of aflatoxins and afla ...
... Mixtures of aflatoxins and aflatoxin B1 have been tested extensively for carcinogenicity by various routes of administration in several strains of mice and rats, in hamsters, several strains of fish, ducks, tree shrews and monkeys. Following their oral administration, mixtures of aflatoxins and afla ...
REDESIGN OF CARNITINE ACETYLTRANSFERASE SPECIFICITY BY PROTEIN ENGINEERING UNIVERSIDAD DE BARCELONA
... Lower dilutions of the primary antibody (1:3,000 and 1:6,000) resulted in a stronger signal, but at the same time produced a higher background signal and non-specific bands (data not shown). Anti-rat CrAT antibodies are specific to rat CrAT and they do not recognise yeastexpressed rat COT (Fig. 3, l ...
... Lower dilutions of the primary antibody (1:3,000 and 1:6,000) resulted in a stronger signal, but at the same time produced a higher background signal and non-specific bands (data not shown). Anti-rat CrAT antibodies are specific to rat CrAT and they do not recognise yeastexpressed rat COT (Fig. 3, l ...
(LPT1) and humans (LPCAT3)
... the human MBOAT5, a protein with unknown function. Cell lysates from insect cells expressing human MBOAT5 (renamed as LPCAT3) showed a 10 fold increase in LPCAT activity over control. In summary, the identification of a novel LPLAT in yeast has proved to be the founding member of LPLAT family in hum ...
... the human MBOAT5, a protein with unknown function. Cell lysates from insect cells expressing human MBOAT5 (renamed as LPCAT3) showed a 10 fold increase in LPCAT activity over control. In summary, the identification of a novel LPLAT in yeast has proved to be the founding member of LPLAT family in hum ...
Short-term regulation of the mammalian pyruvate dehydrogenase
... also exerts an inhibitory influence on PDHK (Bao et al. 2004a). Isoform PDHK2 is more sensitive to inhibition by pyruvate and stimulation by NADH and acetyl-CoA than the other ones (Harris et al., 2002; Bao et al., 2004a). Competitive inhibition of PDHK by ADP versus ATP was also considered to be ess ...
... also exerts an inhibitory influence on PDHK (Bao et al. 2004a). Isoform PDHK2 is more sensitive to inhibition by pyruvate and stimulation by NADH and acetyl-CoA than the other ones (Harris et al., 2002; Bao et al., 2004a). Competitive inhibition of PDHK by ADP versus ATP was also considered to be ess ...
C H A P
... of the lipase(s) produced by this bacterium remain almost unknown, since only related enzymes such as H. pylori phospholipases A2 and C have been cloned and/or characterized (Weitkamp et al., 1993; Dorrell et al., 1999; General Introduction 3.4.2). Therefore, this chapter is focused on the isolation ...
... of the lipase(s) produced by this bacterium remain almost unknown, since only related enzymes such as H. pylori phospholipases A2 and C have been cloned and/or characterized (Weitkamp et al., 1993; Dorrell et al., 1999; General Introduction 3.4.2). Therefore, this chapter is focused on the isolation ...
Iron mediated methylthiolation of tRNA as a regulator of operon
... at 37°C under 5% CO 2 ~95% air (1). Again ovotransferrin was omitted when labelling tRNA from iron replete organisms . Inocula were grown in the same medium containing unlabelled L-methionine (0.01 mg/ml). E. ooli 0111 tRNA was labelled with 3 5 S using 3 5 S O 4 (10 Ci/ 200 ml) as described by Brow ...
... at 37°C under 5% CO 2 ~95% air (1). Again ovotransferrin was omitted when labelling tRNA from iron replete organisms . Inocula were grown in the same medium containing unlabelled L-methionine (0.01 mg/ml). E. ooli 0111 tRNA was labelled with 3 5 S using 3 5 S O 4 (10 Ci/ 200 ml) as described by Brow ...
Structure and mechanism of inhibition of plant
... no similarity between these herbicides and the substrate or product, so binding to the active site appeared to be unlikely. Moreover, there are superficial similarities only between sulfonylureas and imidazolinones and any shared structural elements are also found in a vast range of compounds that d ...
... no similarity between these herbicides and the substrate or product, so binding to the active site appeared to be unlikely. Moreover, there are superficial similarities only between sulfonylureas and imidazolinones and any shared structural elements are also found in a vast range of compounds that d ...
Summary
... nasopharyngeal brushings. The study included a total of 217 individuals with various head and neck complaints selected during year 2007-2010 and demonstrated a 34.6% seroprevalence based on EBV IgA serology. After IgG immunoblot and viral load confirmation, further clinical investigation identified ...
... nasopharyngeal brushings. The study included a total of 217 individuals with various head and neck complaints selected during year 2007-2010 and demonstrated a 34.6% seroprevalence based on EBV IgA serology. After IgG immunoblot and viral load confirmation, further clinical investigation identified ...
Proof-of-principle rapid noninvasive prenatal diagnosis
... To accomplish this goal, we first sequenced GBA-flanking SNPs (up to ±250 kb distance from GBA) of the parents and their firstdegree relatives in families 1 and 2, so as to construct parental haplotypes. However, these family-based haplotypes were of limited size (Supplemental Table 4). Therefore, w ...
... To accomplish this goal, we first sequenced GBA-flanking SNPs (up to ±250 kb distance from GBA) of the parents and their firstdegree relatives in families 1 and 2, so as to construct parental haplotypes. However, these family-based haplotypes were of limited size (Supplemental Table 4). Therefore, w ...
Array-based sequencing of filaggrin gene for
... The filaggrin gene (FLG) is essential for skin differentiation and epidermal barrier formation with links to skin diseases, however it has a highly repetitive nucleotide sequence containing very limited stretches of unique nucleotides for precise mapping to reference genomes. Sequencing strategies u ...
... The filaggrin gene (FLG) is essential for skin differentiation and epidermal barrier formation with links to skin diseases, however it has a highly repetitive nucleotide sequence containing very limited stretches of unique nucleotides for precise mapping to reference genomes. Sequencing strategies u ...
Lab Manual - GRCC Instructional Home Page
... selection in 1859), or modern molecular biologists such as James Watson and Francis Crick (discovered the structure of DNA in 1953), have certain traits in common. They had inquiring minds, great powers of observation, and they used a systematic approach first developed by Roger Bacon an English mon ...
... selection in 1859), or modern molecular biologists such as James Watson and Francis Crick (discovered the structure of DNA in 1953), have certain traits in common. They had inquiring minds, great powers of observation, and they used a systematic approach first developed by Roger Bacon an English mon ...
BIOLOGY 3 Lab Manual - Los Angeles Mission College
... pickups, SUVs) and they may not all give the same result. Plus some cars have 4 adjustable windows and others only 2, and windows can be partially open as well. To keep the experiment simple you might choose to test one type of car that seems “typical” such as a 4-door sedan, and you might limit the ...
... pickups, SUVs) and they may not all give the same result. Plus some cars have 4 adjustable windows and others only 2, and windows can be partially open as well. To keep the experiment simple you might choose to test one type of car that seems “typical” such as a 4-door sedan, and you might limit the ...
Autotrophic CO2 fixation via the reductive tricarboxylic acid cycle in
... genome sequence (A. aeolicus) or carbon isotopic measurements (Tc. ruber) both organisms were previously suspected to use this pathway, but direct evidence had been lacking (Deckert et al., 1998; Jahnke et al., 2001). Isocitrate dehydrogenase, as well as malate dehydrogenase, was found to be NAD(H)- ...
... genome sequence (A. aeolicus) or carbon isotopic measurements (Tc. ruber) both organisms were previously suspected to use this pathway, but direct evidence had been lacking (Deckert et al., 1998; Jahnke et al., 2001). Isocitrate dehydrogenase, as well as malate dehydrogenase, was found to be NAD(H)- ...
Chapter 4: Cellular Metabolism
... (Outcome 4.15) 4. New nucleotides pair with ____________________________ . (Outcome 4.15) 5. DNA polymerase catalyzes ___________________________ . (Outcome 4.15) 6. Each new DNA molecule is composed of one ____ strand and one ____________________________________________________________ strand. D. G ...
... (Outcome 4.15) 4. New nucleotides pair with ____________________________ . (Outcome 4.15) 5. DNA polymerase catalyzes ___________________________ . (Outcome 4.15) 6. Each new DNA molecule is composed of one ____ strand and one ____________________________________________________________ strand. D. G ...
Par-1
... machinery Many components of RNAi machinery have been identified through genetic screening for RNAi defective mutants and through biochemical studies using cell extracts (e.g. Drosophila embryo extract). ...
... machinery Many components of RNAi machinery have been identified through genetic screening for RNAi defective mutants and through biochemical studies using cell extracts (e.g. Drosophila embryo extract). ...
Selecting Degenerate Multiplex PCR Primers
... To overcome the difficulty caused by the NP-hardness of MDPD problems, we propose an iterative beam search algorithm to make a tradeoff between optimality and tractability. In order to solve PT-MDPD and TT-MDPD, MIPS can run in either of two modes, MIPS-PT and MIPS-TT, respectively. This section foc ...
... To overcome the difficulty caused by the NP-hardness of MDPD problems, we propose an iterative beam search algorithm to make a tradeoff between optimality and tractability. In order to solve PT-MDPD and TT-MDPD, MIPS can run in either of two modes, MIPS-PT and MIPS-TT, respectively. This section foc ...
Deoxyribozyme
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Deoxyribozymes, also called DNA enzymes, DNAzymes, or catalytic DNA, are DNA oligonucleotides that are capable of catalyzing specific chemical reactions, similar to the action of other biological enzymes, such as proteins or ribozymes (enzymes composed of RNA).However, in contrast to the abundance of protein enzymes in biological systems and the discovery of biological ribozymes in the 1980s,there are no known naturally occurring deoxyribozymes.Deoxyribozymes should not be confused with DNA aptamers which are oligonucleotides that selectively bind a target ligand, but do not catalyze a subsequent chemical reaction.With the exception of ribozymes, nucleic acid molecules within cells primarily serve as storage of genetic information due to its ability to form complementary base pairs, which allows for high-fidelity copying and transfer of genetic information. In contrast, nucleic acid molecules are more limited in their catalytic ability, in comparison to protein enzymes, to just three types of interactions: hydrogen bonding, pi stacking, and metal-ion coordination. This is due to the limited number of functional groups of the nucleic acid monomers: while proteins are built from up to twenty different amino acids with various functional groups, nucleic acids are built from just four chemically similar nucleobases. In addition, DNA lacks the 2'-hydroxyl group found in RNA which limits the catalytic competency of deoxyribozymes even in comparison to ribozymes.In addition to the inherent inferiority of DNA catalytic activity, the apparent lack of naturally occurring deoxyribozymes may also be due to the primarily double-stranded conformation of DNA in biological systems which would limit its physical flexibility and ability to form tertiary structures, and so would drastically limit the ability of double-stranded DNA to act as a catalyst; though there are a few known instances of biological single-stranded DNA such as multicopy single-stranded DNA (msDNA), certain viral genomes, and the replication fork formed during DNA replication. Further structural differences between DNA and RNA may also play a role in the lack of biological deoxyribozymes, such as the additional methyl group of the DNA base thymidine compared to the RNA base uracil or the tendency of DNA to adopt the B-form helix while RNA tends to adopt the A-form helix. However, it has also been shown that DNA can form structures that RNA cannot, which suggests that, though there are differences in structures that each can form, neither is inherently more or less catalytic due to their possible structural motifs.