The Enolase Superfamily: A General Strategy for Enzyme
... expand families defined on the basis of conserved catalytic properties to include enzymes that catalyze different overall reactions yet share the same structural framework. Comparison of structures in the context of sequence information allows the requirements for catalysis to be identified in a way ...
... expand families defined on the basis of conserved catalytic properties to include enzymes that catalyze different overall reactions yet share the same structural framework. Comparison of structures in the context of sequence information allows the requirements for catalysis to be identified in a way ...
Chemical Modifications and Kinetic Study of Ribonuclease Sa Active
... progress curves were registered at different initial concentrations of the substrate. Kinetic constants kc, and Km were obtained from plots of Lineweaver and Burk (1934). As a substrate for inhibition constant measurements Guo-P-Cyd was used. ...
... progress curves were registered at different initial concentrations of the substrate. Kinetic constants kc, and Km were obtained from plots of Lineweaver and Burk (1934). As a substrate for inhibition constant measurements Guo-P-Cyd was used. ...
Chapter 1 Introduction
... 1.3 Chromosome banding and nomenclature At first the chromosome pairs were classified into seven different groups (A-G), based on morphology (Patau, 1960). A system of nomenclature was proposed at a conference in Denver (Denver Conference, 1960). This classification was officially approved at a conf ...
... 1.3 Chromosome banding and nomenclature At first the chromosome pairs were classified into seven different groups (A-G), based on morphology (Patau, 1960). A system of nomenclature was proposed at a conference in Denver (Denver Conference, 1960). This classification was officially approved at a conf ...
File Ref.No.7054/GA - IV - J1/2013/CU UNIVERSITY OF CALICUT
... enzyme reaction. Derivation of Michaelis-Menten equation and Michaelis-Mention constant (KM). Determination of KM by Lineweaver- Burk plot (for single-substrate enzyme catalyzed reaction). Significance and relevance of KM ...
... enzyme reaction. Derivation of Michaelis-Menten equation and Michaelis-Mention constant (KM). Determination of KM by Lineweaver- Burk plot (for single-substrate enzyme catalyzed reaction). Significance and relevance of KM ...
Heterozygote Advantage and the Evolution of a Dominant
... animals and seed plants, the diploid phase is dominant, and the haploid phase is reduced to one, or a very few cells, which are dependent on the diploid form. In other eukaryotic species, however, the haploid phase may dominate or the phases may be equally developed. Even though an alternation betwe ...
... animals and seed plants, the diploid phase is dominant, and the haploid phase is reduced to one, or a very few cells, which are dependent on the diploid form. In other eukaryotic species, however, the haploid phase may dominate or the phases may be equally developed. Even though an alternation betwe ...
Population genetics and the modern synthesis of evolutionary theory
... − Mutation: a change in the genetic code − usually a change in the sequence of nucleotide bases in a stretch of DNA − usually caused by an error in copying the DNA during replication − since most DNA is unused “junk DNA”, most mutations have no effect − mutations that do affect a working gene are us ...
... − Mutation: a change in the genetic code − usually a change in the sequence of nucleotide bases in a stretch of DNA − usually caused by an error in copying the DNA during replication − since most DNA is unused “junk DNA”, most mutations have no effect − mutations that do affect a working gene are us ...
Часть 1. - Ассоциация синдрома Ретта
... encompassing the position of pathogenic mutations allowed us to reliably determine the parental origin of the de novo mutations associated with Rett syndrome in seven informative families. Although additional studies are required to reach statistically significant figures, our data suggest a predomi ...
... encompassing the position of pathogenic mutations allowed us to reliably determine the parental origin of the de novo mutations associated with Rett syndrome in seven informative families. Although additional studies are required to reach statistically significant figures, our data suggest a predomi ...
İngilizce Başlık - istanbul üniveristesi veteriner fakültesi dergisi
... not contain donkey or mule meat, similarly absence of chicken meat in a meat product does not mean that this product does not contain turkey or other poultry meat. Therefore, equine primer and poultry primer were used to identification of horse, donkey, and chicken, turkey meat, respectively, instea ...
... not contain donkey or mule meat, similarly absence of chicken meat in a meat product does not mean that this product does not contain turkey or other poultry meat. Therefore, equine primer and poultry primer were used to identification of horse, donkey, and chicken, turkey meat, respectively, instea ...
Annex 1
... (ii) sequences which are included are any unbranched sequences of four or more amino acids or unbranched sequences of ten or more nucleotides. Branched sequences, sequences with fewer than four specifically defined nucleotides or amino acids as well as sequences comprising nucleotides or amino acids ...
... (ii) sequences which are included are any unbranched sequences of four or more amino acids or unbranched sequences of ten or more nucleotides. Branched sequences, sequences with fewer than four specifically defined nucleotides or amino acids as well as sequences comprising nucleotides or amino acids ...
The nucleotide sequence of Saccharomyces cerevisiae
... IX contains 221 open reading frames (ORFs), of which approximately 30% have been sequenced previously. This chromosome shows features typical of a small Saccharomyces cerevisiae chromosome. The sequence derived for chromosome IX is 439,886 nucleotides in length, and 71.6% codes for proteins or predi ...
... IX contains 221 open reading frames (ORFs), of which approximately 30% have been sequenced previously. This chromosome shows features typical of a small Saccharomyces cerevisiae chromosome. The sequence derived for chromosome IX is 439,886 nucleotides in length, and 71.6% codes for proteins or predi ...
Cell-Free Phospholipid Biosynthesis by Gene
... around 83 kDa and 27 kDa (Fig 1b) as previously reported [17,18]. It is known that GPAT is an integral membrane protein [19] and LPAAT is thought to be a membrane-anchored protein [18]. We thus examined the ability of the synthesized enzymes to associate to the liposome membrane. Preformed small uni ...
... around 83 kDa and 27 kDa (Fig 1b) as previously reported [17,18]. It is known that GPAT is an integral membrane protein [19] and LPAAT is thought to be a membrane-anchored protein [18]. We thus examined the ability of the synthesized enzymes to associate to the liposome membrane. Preformed small uni ...
Assessment of the mosaic structure in the
... sequencing and CGE analysis also revealed a bias of amplicons observed before (Fig. 2, lane 18) and after cloning (Fig. 2, lane 18a and 18h) which might be due to the fact that only highly abundant amplicons, present in the initial amplification derived from H. pylori HJM18MDA-DNA, were detectable b ...
... sequencing and CGE analysis also revealed a bias of amplicons observed before (Fig. 2, lane 18) and after cloning (Fig. 2, lane 18a and 18h) which might be due to the fact that only highly abundant amplicons, present in the initial amplification derived from H. pylori HJM18MDA-DNA, were detectable b ...
LIN-28 co-transcriptionally binds primary let
... MicroRNAs (miRNAs) function as ~22-nucleotide (nt) guide RNAs in the RNA-induced silencing complex (RISC) by binding to partially complementary sites in target mRNAs, causing inhibition of translation or destabilization. Let-7 miRNA that originally discovered in C. elegans is conserved across specie ...
... MicroRNAs (miRNAs) function as ~22-nucleotide (nt) guide RNAs in the RNA-induced silencing complex (RISC) by binding to partially complementary sites in target mRNAs, causing inhibition of translation or destabilization. Let-7 miRNA that originally discovered in C. elegans is conserved across specie ...
Experimental studies of ploidy evolution in yeast
... gametes during the haploid phase of their life cycles, so their gametes are generated by mitosis and are identical to each other. The hypothesized advantage of diploidy here is that it may help to diversify your entries in the contest that is the next generation. This would be a particular advantage ...
... gametes during the haploid phase of their life cycles, so their gametes are generated by mitosis and are identical to each other. The hypothesized advantage of diploidy here is that it may help to diversify your entries in the contest that is the next generation. This would be a particular advantage ...
Insights into antibody catalysis: Structure of an oxygenation
... Data Collection. Data sets were collected at - 165°C (Fabhapten) and 4°C (free Fab) using an R-AXIS II detector system mounted on a Rigaku RU-200 x-ray generator (50 kV and 100 mA). The reflections were indexed using DENZO 1.3.0 (written by Z. Otwinowski) and merged/scaled using the programs ROTAVAT ...
... Data Collection. Data sets were collected at - 165°C (Fabhapten) and 4°C (free Fab) using an R-AXIS II detector system mounted on a Rigaku RU-200 x-ray generator (50 kV and 100 mA). The reflections were indexed using DENZO 1.3.0 (written by Z. Otwinowski) and merged/scaled using the programs ROTAVAT ...
North America
... of nucleic acid or carryover of PCR inhibitors, absence of sufficient human cells in the sample, improper assay set up and execution and reagent/equipment malfunction. • IF RP is negative – Repeat sample for all targets – HOWEVER, if for a specific sample, RP is negative but the pathogenspecific tar ...
... of nucleic acid or carryover of PCR inhibitors, absence of sufficient human cells in the sample, improper assay set up and execution and reagent/equipment malfunction. • IF RP is negative – Repeat sample for all targets – HOWEVER, if for a specific sample, RP is negative but the pathogenspecific tar ...
Fragaria multicipita - DigitalCommons@University of Nebraska
... standards used were PhiX174 RF HaeIII digest (Life Technologies) and 1.6 kb ladder (Fermentas AB, Vilnius, Lithuania). RFLP patterns were compared with those previously published (Davis et al., 1997; Jomantiene et al. 1998a,b, 2002; Lee et al. 1998). Phytoplasma 16S rRNA group and subgroup designati ...
... standards used were PhiX174 RF HaeIII digest (Life Technologies) and 1.6 kb ladder (Fermentas AB, Vilnius, Lithuania). RFLP patterns were compared with those previously published (Davis et al., 1997; Jomantiene et al. 1998a,b, 2002; Lee et al. 1998). Phytoplasma 16S rRNA group and subgroup designati ...
Thesis-1965R-K29r
... composition of the nucleolus is chiefly RNA and proteins. It may also contain certain enzymes and I ipids. The synthesis of RNA in the nucleolus is generally accepted. The types are not agreed on. The transfer of RNA to the cytoplasm seems probable but the method is not apparent. Protein synthesis w ...
... composition of the nucleolus is chiefly RNA and proteins. It may also contain certain enzymes and I ipids. The synthesis of RNA in the nucleolus is generally accepted. The types are not agreed on. The transfer of RNA to the cytoplasm seems probable but the method is not apparent. Protein synthesis w ...
Contribution of IKBKE and IFIH1 gene variants to SLE susceptibility
... Figure 1. The IKBKE SNV rs12142086 affects the binding of SF1 with DNA. (a) The SNV rs12142086 is predicted to be located in the binding motif of SF1. Introns are represented by grey horizontal lines and exons by vertical bars with numbers. The position of rs12142086 is highlighted with a star. In t ...
... Figure 1. The IKBKE SNV rs12142086 affects the binding of SF1 with DNA. (a) The SNV rs12142086 is predicted to be located in the binding motif of SF1. Introns are represented by grey horizontal lines and exons by vertical bars with numbers. The position of rs12142086 is highlighted with a star. In t ...
Killing specimens
... lids work for smaller situations. Be sure to include adequate space for emerging specimens to expand their wings. Also be sure that specimens are not able to chew their way out of the container. 3. Food: If possible, host plant material should be exchanged regularly with fresh material. For internal ...
... lids work for smaller situations. Be sure to include adequate space for emerging specimens to expand their wings. Also be sure that specimens are not able to chew their way out of the container. 3. Food: If possible, host plant material should be exchanged regularly with fresh material. For internal ...
VECTOR SYSTEMS XXVII.indd
... Our Bacillus megaterium kit is a new easy-to-handle system for stable protein expression with high yield. It is not only suited for industrial large scale protein production, but offers also an interesting alternative to the standard host E. coli. The kit comes with the E. coli/Bacillus megaterium s ...
... Our Bacillus megaterium kit is a new easy-to-handle system for stable protein expression with high yield. It is not only suited for industrial large scale protein production, but offers also an interesting alternative to the standard host E. coli. The kit comes with the E. coli/Bacillus megaterium s ...
Deoxyribozyme
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Deoxyribozymes, also called DNA enzymes, DNAzymes, or catalytic DNA, are DNA oligonucleotides that are capable of catalyzing specific chemical reactions, similar to the action of other biological enzymes, such as proteins or ribozymes (enzymes composed of RNA).However, in contrast to the abundance of protein enzymes in biological systems and the discovery of biological ribozymes in the 1980s,there are no known naturally occurring deoxyribozymes.Deoxyribozymes should not be confused with DNA aptamers which are oligonucleotides that selectively bind a target ligand, but do not catalyze a subsequent chemical reaction.With the exception of ribozymes, nucleic acid molecules within cells primarily serve as storage of genetic information due to its ability to form complementary base pairs, which allows for high-fidelity copying and transfer of genetic information. In contrast, nucleic acid molecules are more limited in their catalytic ability, in comparison to protein enzymes, to just three types of interactions: hydrogen bonding, pi stacking, and metal-ion coordination. This is due to the limited number of functional groups of the nucleic acid monomers: while proteins are built from up to twenty different amino acids with various functional groups, nucleic acids are built from just four chemically similar nucleobases. In addition, DNA lacks the 2'-hydroxyl group found in RNA which limits the catalytic competency of deoxyribozymes even in comparison to ribozymes.In addition to the inherent inferiority of DNA catalytic activity, the apparent lack of naturally occurring deoxyribozymes may also be due to the primarily double-stranded conformation of DNA in biological systems which would limit its physical flexibility and ability to form tertiary structures, and so would drastically limit the ability of double-stranded DNA to act as a catalyst; though there are a few known instances of biological single-stranded DNA such as multicopy single-stranded DNA (msDNA), certain viral genomes, and the replication fork formed during DNA replication. Further structural differences between DNA and RNA may also play a role in the lack of biological deoxyribozymes, such as the additional methyl group of the DNA base thymidine compared to the RNA base uracil or the tendency of DNA to adopt the B-form helix while RNA tends to adopt the A-form helix. However, it has also been shown that DNA can form structures that RNA cannot, which suggests that, though there are differences in structures that each can form, neither is inherently more or less catalytic due to their possible structural motifs.