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... squid (Dosidicus gigas), in addition to being the largest known cephalopod, presents a high amount of byproducts produced during its processing (skin, fins, arms, and head) (Lin & Li, 2006), which have collagen as the most prevalent protein (Alemán et al., 2011a; Gildberg et al., 2002). Collagen has ...
Slide 1
Slide 1

... Some are stable Some are not... they 'decay' - lose the neutron These 'radioisotopes' emit energy (radiation) This process is not affected by environmental conditions and is constant; so if we know the amount of parent and daughter isotope, and we know the decay rate, we can calculate the time it ha ...
A Human Centromere Protein, CENP-B, Has a DNA Binding Domain
A Human Centromere Protein, CENP-B, Has a DNA Binding Domain

... Steuer et al., 1990). Several monoclonal antibodies that recognize the centromeric region of human chromosomes have been isolated using scaffold proteins as antigens (Cooke et al., 1987; Compton et al., 1991); one of the antigens, a 250300-kD protein, was found to be localized to centromere only at ...
Neonatal nutrition
Neonatal nutrition

... • Try to get baby onto the breast within first 1-2 hours of life • Colestrum ; high in protein a nd immunoglobuline ...
Fatty acid and phospholipid metabolism in prokaryotes
Fatty acid and phospholipid metabolism in prokaryotes

Vacuolar Sorting Receptor-Mediated Trafficking of Soluble Vacuolar
Vacuolar Sorting Receptor-Mediated Trafficking of Soluble Vacuolar

... these atvsr mutants have smaller PSVs than the wild type, which may be due to the reduced levels of PSV proteins in the PSVs resulting from defects in PSV trafficking. However, single and double mutants of atvrs1, atvsr3, and atvsr4 do not show any obvious defective phenotype in their vegetative tis ...
1 - Plant Research International
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... The method of entry into pathway 2 will be by pulse labelling of the roots of rooted cloves in nutrient medium containing 35SO4 followed by analysis of the pattern of labelling in the leaves. Particular attention will be paid to the labelling in the S-allyl--glutamyl cysteine and S-allyl cysteine. ...
9. steroid hormones - cmb
9. steroid hormones - cmb

... •  StAR is important in transferring cholesterol, the precursor for all steroid hormones, from the outer to inner mitochondrial membrane where P450scc is located •  P450scc is a side chain cleavage enzyme which converts cholesterol to pregnenolone, and is the rate-limiting enzyme in steroidogenesis ...
Chapter 1. introduction
Chapter 1. introduction

... 8.3. Reactions of monosaccharides A. Reducing sugars: Reduction of Cu+2 to Cu +1 B. Lactone formation: intermolecular ester C. Deoxy sugars: Another form of reduced sugars D. Esterification with phosphoric group ( ATP) ...
Soil Biology and Biochemistry
Soil Biology and Biochemistry

... of ammonium sulfate saturation) [Fig. 2 (C)l. Tryptophan was used as an aromatic amino acid donor, and 2-oxoglutaric acid as the amino group acceptor in the reaction mixture. Specific activities in extracts from cultures without amino acids ranged from 16 to 22 nmol of IPyA mgg’ protein ml-‘. Howeve ...
Correlation of an Immobilized Digestive Enzyme Assay with Poultry
Correlation of an Immobilized Digestive Enzyme Assay with Poultry

... ingredients would enable the formulation of diets that more closely meet amino acid requirements. In vitro and in vivo techniques exist to monitor the quality of proteins in feedstuffs. One of the most common in vivo methods to determine true amino acid digestibility (TAAD) is the precision-fed cece ...
METBIONET GUIDELINES FOR AMINO ACID ANALYSIS.
METBIONET GUIDELINES FOR AMINO ACID ANALYSIS.

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FORMATION OF AMMONIA

The N Terminus of Bacterial Elongation Factor Tu
The N Terminus of Bacterial Elongation Factor Tu

... As a first step of purification, crude bacterial extract was fractionated on a MonoQ ion exchange column. Activity eluted as a single peak, and proteins in fractions with elicitor activity were pooled and proteins precipitated by 80% acetone and separated by SDS-PAGE (Figure 2A). After staining and ...
October 15 AP Biology - John D. O`Bryant School of Math & Science
October 15 AP Biology - John D. O`Bryant School of Math & Science

...  Enzyme concentration  Substrate concentration  Temperature  pH  Salinity  Activators  Inhibitors AP Biology ...
Biochimie
Biochimie

... lipolysis (Fig. 1). Modification of fatty acid chain length and saturation levels to produce monounsaturated and saturated TAG, the ideal form for biodiesel, can be further achieved via expression of thioesterases and silencing of desaturases. These strategies are based on the recently obtained thoro ...
The Effects of Exogenous Amino Acids on Growth
The Effects of Exogenous Amino Acids on Growth

Enzymes at work
Enzymes at work

... in industrial processes, listed according to class, for example: ...
meat quality differences between purebred and crossbred new
meat quality differences between purebred and crossbred new

... New Zealand White and Hyplus rabbits are used in commercial crossbreeding with different breeds. After having crossbred several different breeds we can get the effect of heterosis (Masoero 1982, Rochambeau 1988). However, it is proved that not every interbreeding and interlinear crossbreeding can be ...
Functional lipidomics of oxidized products from polyunsaturated fatty
Functional lipidomics of oxidized products from polyunsaturated fatty

... lipoxygenases, is a process that leads to hydroperoxides specifically transformed into a series of more stable metabolites called eicosanoids, docosanoids and even octadecanoids from C20, C22 and C18 PUFA, respectively. Functional lipidomics may be seen as targeted lipidomics addressed to classes of ...
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Zhan-3-Enzyme

... • For example: elevated levels of insulin cause an increase in the synthesis of key enzymes involved in glucose metabolism ...
How to move an amphipathic molecule across a lipid
How to move an amphipathic molecule across a lipid

Chapter 7 - Medical Image Analysis
Chapter 7 - Medical Image Analysis

... only modulated by means of phosphorylation/dephosphorylation, but also by translocation from one subcellular compartment to another upon insulin-stimulation or exercise. For example, we have known for many years that one of the key steps in glucose uptake is the translocation of GLUT4 from intracell ...
COMPLEX FORMATION AND PROTEIN INTERACTION IN THE
COMPLEX FORMATION AND PROTEIN INTERACTION IN THE

... interactions between all CesAs within a CSC, utilizing techniques such as analytical ultracentrifugation, isothermal titration calorimetry (ITC), and dynamic light scattering (DLS). Additionally, site-directed mutagenesis was used to identify residues important for these proteinprotein interactions. ...
Fundamentals: Bioenergetics and Enzyme Function
Fundamentals: Bioenergetics and Enzyme Function

... Metabolic Design 1. Why is the free energy release of ATP hydrolysis moderate in amount compared to other phosphate containing molecules? 2. If anabolism increases the size, complexity and order of the body, which by definition means deceasing entropy in the body, how can these reactions occur witho ...
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Proteolysis



Proteolysis is the breakdown of proteins into smaller polypeptides or amino acids. Uncatalysed, the hydrolysis of peptide bonds is extremely slow, taking hundreds of years. Proteolysis is typically catalysed by cellular enzymes called proteases, but may also occur by intra-molecular digestion. Low pH or high temperatures can also cause proteolysis non-enzymatically.Proteolysis in organisms serves many purposes; for example, digestive enzymes break down proteins in food to provide amino acids for the organism, while proteolytic processing of a polypeptide chain after its synthesis may be necessary for the production of an active protein. It is also important in the regulation of some physiological and cellular processes, as well as preventing the accumulation of unwanted or abnormal proteins in cells. Consequently, dis-regulation of proteolysis can cause diseases, and is used in some venoms to damage their prey.Proteolysis is important as an analytical tool for studying proteins in the laboratory, as well as industrially, for example in food processing and stain removal.
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