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Influence of milk source and ripening time on free amino
Influence of milk source and ripening time on free amino

... derived thereof) that are detected by polyacrylamide gel electrophoresis (Rank et al., 1985); products of secondary proteolysis in cheese encompass small peptides and free amino acids that are soluble in the aqueous phase of cheese and are thus recovered in the water-soluble fraction. In Picante che ...
0 - Microbiology
0 - Microbiology

... Rate of glutamate formation. A mixture containing leucine, a-ketoghztarate and dialysed acetone powder extract was incubated and the glutamate formed measured after various intervals. Under the conditions used, the amount of glutamate formed increased continuously during the whole course of the expe ...
The Organellar Genome and Metabolic Potential
The Organellar Genome and Metabolic Potential

... cockroach species. It has numerous hydrogen-producing organelles that are intimately associated with endosymbiotic methane–producing archaea that use the hydrogen produced by the organelles. Despite the hydrogenosomal metabolism of this organelle, we have found that it actually has a genome (Akhmano ...
enzymes - AP Bio Take 5
enzymes - AP Bio Take 5

...  Biological catalysts ...
An intersubunit lock-and-key `Clasp` motif in the dimer interface of
An intersubunit lock-and-key `Clasp` motif in the dimer interface of

... in the other subunit. This interaction was reported to be specific to mammalian Alpha/Mu/Pi classes [16–19]. The second type of intersubunit interaction is more hydrophilic and lacks the ‘lockand-key’ motif, as in Theta/Sigma classes [20,21]. The classspecific intersubunit interactions result in dif ...
Chapter 25 Amino Acids, Peptides, and Proteins
Chapter 25 Amino Acids, Peptides, and Proteins

... Amino acids in which the two functional groups are separated by exactly one carbon atom are called _______ amino acids. Amino acids are coupled together by amide linkages called ____________ bonds. Relatively short chains of amino acids are called ___________. Only twenty amino acids are abundantly ...
Enzymes and Metabolism
Enzymes and Metabolism

...  Biological catalysts ...
Metabolism & Enzymes
Metabolism & Enzymes

...  Biological catalysts ...
Enzymes for Cell Dissociation and Lysis - Sigma
Enzymes for Cell Dissociation and Lysis - Sigma

... activity that assists the digestion of animal tissue. Because the clostripain present in crude collagenase must be reduced (e.g., by treatment with dithiothreitol) in order to be active this enzyme probably contributes little to the tissue dissociation process in the laboratory. It is monitored beca ...
Genomics Insights esTs from seeds to Assist the selective Breeding
Genomics Insights esTs from seeds to Assist the selective Breeding

... C16 fatty acid (palmitate) is synthesized. Fatty acids chains longer than palmitate are formed by elongation reactions catalyzed by enzymes on the cytosolic side of the endoplasmic reticulum ­membrane. These reactions add two carbon units (Malonyl CoA) sequentially to the carboxyl ends of both satur ...
Markers of renal function tests (PDF Available)
Markers of renal function tests (PDF Available)

... radioactive and non radioactive. They indicate the glomerular filtration rate, concentrating and diluting capacity of kidneys (tubular function). If there is an increase or decrease in the valves of these markers it indicates dysfunction of kidney. Aim: The aim of this review is to compare and analy ...
Sequence Queries
Sequence Queries

... The error tolerant tag allows for this. In effect, it allows the peptide mass to vary and allows the tag to float. However, the tag must stay attached to one end or the other. Either both fragment ion masses are unchanged or both fragment ion masses shift by the same amount as the precursor. This ca ...
Metabolomic Profiling Identifies Biomarkers Associated
Metabolomic Profiling Identifies Biomarkers Associated

... – For use in models with other metabolites and metabolic parameters for: l The prediction of type 2 diabetes l Monitoring interventions in diabetes and prediabetes ...
In Vitro Reconstitution of SCF Substrate Ubiquitination with Purified
In Vitro Reconstitution of SCF Substrate Ubiquitination with Purified

... successfully to study how SCF recognizes phosphorylated substrates (Nash et al., 2001; Orlicky et al., 2003; Verma et al., 1997a), the mechanisms of ubiquitin transfer through SCF (Deffenbaugh et al., 2003), the minimum set of components that can sustain Sic1 turnover and S-CDK activation (Verma et ...
Structure and Function of Large Bio Molecules
Structure and Function of Large Bio Molecules

... An immense variety of polymers can be built from a small number of monomers. • Each cell has thousands of different kinds of macromolecules. • Macromolecules vary among cells of the same individual. They vary more among unrelated individuals of a species, and even more between species. • This divers ...
Ubiquinone
Ubiquinone

... diabetes. ...
Document
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... • Phosphorylation is the addition of a phosphate (PO4) group to a protein or other organic molecule. • kinases (phosphorylation) and phosphatases (dephosphorylation) are involved in this process. Many enzymes and receptors are switched "on" or "off" by phosphorylation and dephosphorylation. • Revers ...
Studies on legume receptors for Nod and Myc symbiotic signals
Studies on legume receptors for Nod and Myc symbiotic signals

... large number of plant viruses and other pathogens such as bacteria, oomycetes and fungi and thus is widely used in plant virology and for host–pathogen interaction studies. For the reason that its large leaves can be easily transformed with high efficiency with Agrobacterium tumefaciens, N. benthami ...
The phosphopantetheinyl transferases
The phosphopantetheinyl transferases

... through a reactive thioester linkage. PPant is thought of as a “prosthetic arm” on which all substrates and intermediates of these pathways are covalently yet transiently held during the orderly progression of enzymatic modications to the extending chain. PPTases mediate the transfer and covalent a ...
Modulating the cobalt redox potential through imidazole hydrogen
Modulating the cobalt redox potential through imidazole hydrogen

... the cofactor is replaced by a protein histidine (“His”) anchoring group (“His-on” conguration). This mode of protein-cofactor binding is shown in Fig. 1 (ref. 10) and encountered, amongst others, in MeCbl-dependent methionine synthase (MetH) as well as AdoCbl dependent methylmalonyl CoA mutase (MCM ...
8)Discuss the roles of cofactors and coenzymes in enzyme activity.
8)Discuss the roles of cofactors and coenzymes in enzyme activity.

... Many enzymes posses prosthetic groups that are non-amino acid in their nature. Prosthetic group is name reserved for a permanently attached cofactor or coenzyme group.Those conjugated proteins (apoenzyme and cofactor/coenzyme) are called as holoenzymes. A holoenzyme is dissociated into a protein com ...
CHEMISTRY 132
CHEMISTRY 132

... 17. (3 points) In proteins, tertiary structure may be stabilized by the formation of ____________ between 2 Cys residues. a. Hydrogen bonds b. Ionic bonds c. Disulfide bonds d. Hydrophobic interactions ANS: C 18. (3 points) Which of the following is a function of proteins? a. catalytic b. protective ...
Lecture 9 Fatty Acid Synthesis
Lecture 9 Fatty Acid Synthesis

... Malonyl-CoA by Acetyl-CoA Carboxylase (ACC) Biotin: water soluble vitamin – functions as a CO2 carrier for several important reactions including:  Acetyl-CoA carboxylase  Pyruvate carboxylase  Propionyl CoA carboxylase ...
SharifMZ_0808_eps - Heriot
SharifMZ_0808_eps - Heriot

... Although L-glutamate production by Corynebacteria fermentation has been wellestablished in the biotechnology industry since 1960’s, the demand for this building block for human consumption is increasing enormously. Researchers have therefore been trying to improve the yield and productivity of amino ...
1 CHAPTER I INTRODUCTION The nucleus of the cell contains our
1 CHAPTER I INTRODUCTION The nucleus of the cell contains our

... secondary structure termed the histone-fold motif, consisting of a general helix-loophelix-loop-helix domain (Figure 5) (49, 50). The strong tertiary structure in the histone core created by anti-parallel histone-fold binding of dimers, and hydrogen bonding association (through the phosphate backbon ...
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Proteolysis



Proteolysis is the breakdown of proteins into smaller polypeptides or amino acids. Uncatalysed, the hydrolysis of peptide bonds is extremely slow, taking hundreds of years. Proteolysis is typically catalysed by cellular enzymes called proteases, but may also occur by intra-molecular digestion. Low pH or high temperatures can also cause proteolysis non-enzymatically.Proteolysis in organisms serves many purposes; for example, digestive enzymes break down proteins in food to provide amino acids for the organism, while proteolytic processing of a polypeptide chain after its synthesis may be necessary for the production of an active protein. It is also important in the regulation of some physiological and cellular processes, as well as preventing the accumulation of unwanted or abnormal proteins in cells. Consequently, dis-regulation of proteolysis can cause diseases, and is used in some venoms to damage their prey.Proteolysis is important as an analytical tool for studying proteins in the laboratory, as well as industrially, for example in food processing and stain removal.
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