Welcome to Techniques in Molecular Biology
... Detection of Proteins • antibody probes (detected by secondary antibody linked to an enzyme or a fluorescent tag) – immunization with whole protein • purification of protein – from tissues or cultured cells – recombinant protein – DNA immunization of birds - IgY – epitope tags ...
... Detection of Proteins • antibody probes (detected by secondary antibody linked to an enzyme or a fluorescent tag) – immunization with whole protein • purification of protein – from tissues or cultured cells – recombinant protein – DNA immunization of birds - IgY – epitope tags ...
UNIT 4: Chapter 6.1 Yellow Box Questions AK
... A macromolecule is a large, complex assembly of organic molecules. The four categories of macromolecules are carbohydrates, lipids, proteins, and nucleic acids 2. Name and describe and draw the process that builds macromolecules. The process that builds macromolecules is called dehydration synthesis ...
... A macromolecule is a large, complex assembly of organic molecules. The four categories of macromolecules are carbohydrates, lipids, proteins, and nucleic acids 2. Name and describe and draw the process that builds macromolecules. The process that builds macromolecules is called dehydration synthesis ...
Ch. 5 "The Structure and Function of Macromolecules
... ----------Macromolecules---------1. Describe how covalent linkages are formed and broken in organic polymers. -----------Carbohydrates---------2. Describe the distinguishing characteristics of carbohydrates, and explain how they are ...
... ----------Macromolecules---------1. Describe how covalent linkages are formed and broken in organic polymers. -----------Carbohydrates---------2. Describe the distinguishing characteristics of carbohydrates, and explain how they are ...
BICH 303 Exam #1 Fall 2005 1. Amphiphilic or amphipathic
... A. the chemical reactivity of some side-chains can be used to modify proteins in specific ways. B. the chemical and physical properties of some side-chains allow detection and quantification. C. the chemical and physical properties of side-chains bestow proteins with specific biological function. D. ...
... A. the chemical reactivity of some side-chains can be used to modify proteins in specific ways. B. the chemical and physical properties of some side-chains allow detection and quantification. C. the chemical and physical properties of side-chains bestow proteins with specific biological function. D. ...
1 OVERVIEW OF EXTRACELLULAR SIGNALING A. Steps of
... A. Steps of extracellular communication 1. synthesis of signaling molecule 2. Release of signaling molecule 3. Transport of the signal to the target cell 4. Detection of the signal by a specific receptor protein 5. Change in cellular metabolism or gene expression triggered by the receptorsignaling m ...
... A. Steps of extracellular communication 1. synthesis of signaling molecule 2. Release of signaling molecule 3. Transport of the signal to the target cell 4. Detection of the signal by a specific receptor protein 5. Change in cellular metabolism or gene expression triggered by the receptorsignaling m ...
15-25 kD
... – Class – Order – Family – Genus – Species • Traditional classification based upon traits: – Morphological – Behavioral ...
... – Class – Order – Family – Genus – Species • Traditional classification based upon traits: – Morphological – Behavioral ...
Chapter 3 The Chemical Building Blocks of Life
... A fat molecule is a triglyceride, containing three fatty acids. Saturated fats have all carbon atoms in the fatty acid chains bonded to at least two hydrogen atoms, having all the hydrogen atoms possible Unsaturated fats have fatty acids with double bounds between pairs of successive carbon atoms. ...
... A fat molecule is a triglyceride, containing three fatty acids. Saturated fats have all carbon atoms in the fatty acid chains bonded to at least two hydrogen atoms, having all the hydrogen atoms possible Unsaturated fats have fatty acids with double bounds between pairs of successive carbon atoms. ...
Protein Synthesis - mvhs
... REVIEW: PROTEIN SYNTHESIS TERMS Protein Synthesis Transcription Translation DNA Amino acids RNA Polymerase Enzymes Protein Ribosome ...
... REVIEW: PROTEIN SYNTHESIS TERMS Protein Synthesis Transcription Translation DNA Amino acids RNA Polymerase Enzymes Protein Ribosome ...
Lecture_14.pps
... USUAL TURNOVER • Most glycans are extracellular or on cell surface • Membrane recycling • Receptor and non-receptor mediated endocytosis ...
... USUAL TURNOVER • Most glycans are extracellular or on cell surface • Membrane recycling • Receptor and non-receptor mediated endocytosis ...
07-Quiz 3 Key
... b. This structure represents a common vitamin. From the structure, which statement about this vitamin is correct? a. It is expected to be very soluble in fats, making an overdose possible. b. It will playa role in metabolizing minerals in the body, making an overdose ...
... b. This structure represents a common vitamin. From the structure, which statement about this vitamin is correct? a. It is expected to be very soluble in fats, making an overdose possible. b. It will playa role in metabolizing minerals in the body, making an overdose ...
Transduction Kit for Peptides and Proteins
... of the transduction cocktail contain a nuclear localization sequence and are therefore able to transport a cargo into the nucleus. The Kit further contains compounds for increasing rate and efficiency of transduction. DMSO enhances the permeability of cell membranes. BSA protects to some degree the ...
... of the transduction cocktail contain a nuclear localization sequence and are therefore able to transport a cargo into the nucleus. The Kit further contains compounds for increasing rate and efficiency of transduction. DMSO enhances the permeability of cell membranes. BSA protects to some degree the ...
Introduction to the Digestive System Notes
... Plant support: Cellulose (a polysac) provides support for plants ...
... Plant support: Cellulose (a polysac) provides support for plants ...
D. E. Shaw Research is seeking postdoctoral fellows of exceptional... Postdoctoral Fellowships at D. E. Shaw Research
... physics, or in a relevant area of computer science or applied mathematics. Relevant areas of experience might include the study of allosteric interactions or other functionally important conformational changes in biological molecules, structure prediction or design for proteins or RNA, the study of ...
... physics, or in a relevant area of computer science or applied mathematics. Relevant areas of experience might include the study of allosteric interactions or other functionally important conformational changes in biological molecules, structure prediction or design for proteins or RNA, the study of ...
Cells are exposed to DNA damaging agents that can affect their
... do not have any symmetry, especially those of the so-called “molecular machines” where a collection of proteins interact to work in such various processes as DNA repair or RNA splicing. Also, some important molecules in the size range of 100 kDa are multi-domain proteins which are difficult to expre ...
... do not have any symmetry, especially those of the so-called “molecular machines” where a collection of proteins interact to work in such various processes as DNA repair or RNA splicing. Also, some important molecules in the size range of 100 kDa are multi-domain proteins which are difficult to expre ...
de novo Protein Design
... Search of nearby conformational space and sequence space 2 methods of protein redesign (variation of backbone conformation and amino acid sequence) Development of procedure for identifying low free energy sequence-structure pairs that iterates between sequence optimization and structure prediction R ...
... Search of nearby conformational space and sequence space 2 methods of protein redesign (variation of backbone conformation and amino acid sequence) Development of procedure for identifying low free energy sequence-structure pairs that iterates between sequence optimization and structure prediction R ...
Fibrous proteins are especially abundant outside the cell, where
... All proteins stick, or bind, to other molecules. In some cases this binding is very tight; in others it is weak and short-lived. In all cases binding shows great specificity, in the sense that each protein molecule can bind to just or a few molecules out of the many thousands of different molecules ...
... All proteins stick, or bind, to other molecules. In some cases this binding is very tight; in others it is weak and short-lived. In all cases binding shows great specificity, in the sense that each protein molecule can bind to just or a few molecules out of the many thousands of different molecules ...
Chapter 3 Chemistry of Life Modern Biology Textbook Holt
... sugar called a disaccharide such as sucrose (glucose + fructose) • Polysaccharides --Three or more monosaccharide's join to form large macromolecules such as starches, cellulose, chitin & glycogen. These are complex carbohydrates ...
... sugar called a disaccharide such as sucrose (glucose + fructose) • Polysaccharides --Three or more monosaccharide's join to form large macromolecules such as starches, cellulose, chitin & glycogen. These are complex carbohydrates ...
Heat shock protein (Hsp)65-70: dominant self
... ligand in the TcRy8 selection. Over recent years, Hsps, that were initially recognized in Drosophila by the increased expression after exposure to elevated temperatures (assumed to provide the cells with protection during recovery from stress), but that now are identified as structurally conserved e ...
... ligand in the TcRy8 selection. Over recent years, Hsps, that were initially recognized in Drosophila by the increased expression after exposure to elevated temperatures (assumed to provide the cells with protection during recovery from stress), but that now are identified as structurally conserved e ...
3-D Structure of proteins
... • Subunits of oligomer usually held together by noncovalent forces. Usually hydrophobic interactions, but electrostatic forces may also be involved. A large proportion of globular proteins have quaternary structure. Probable reasons are: 1. more stable than dissociated subunits 2. active sites of so ...
... • Subunits of oligomer usually held together by noncovalent forces. Usually hydrophobic interactions, but electrostatic forces may also be involved. A large proportion of globular proteins have quaternary structure. Probable reasons are: 1. more stable than dissociated subunits 2. active sites of so ...
Proteolysis
Proteolysis is the breakdown of proteins into smaller polypeptides or amino acids. Uncatalysed, the hydrolysis of peptide bonds is extremely slow, taking hundreds of years. Proteolysis is typically catalysed by cellular enzymes called proteases, but may also occur by intra-molecular digestion. Low pH or high temperatures can also cause proteolysis non-enzymatically.Proteolysis in organisms serves many purposes; for example, digestive enzymes break down proteins in food to provide amino acids for the organism, while proteolytic processing of a polypeptide chain after its synthesis may be necessary for the production of an active protein. It is also important in the regulation of some physiological and cellular processes, as well as preventing the accumulation of unwanted or abnormal proteins in cells. Consequently, dis-regulation of proteolysis can cause diseases, and is used in some venoms to damage their prey.Proteolysis is important as an analytical tool for studying proteins in the laboratory, as well as industrially, for example in food processing and stain removal.