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BIO120 LAB --DNA + PROTEIN SYN-
BIO120 LAB --DNA + PROTEIN SYN-

... • New nucleotides are added/paired with the existing strands • DNA polymerase binds the new nucleotides together creating the P-S backbone • Result is two identical DNA molecules (i.e., the base sequence is the same) ...
BamHI
BamHI

... • After the agarose solidifies, the comb is removed leaving wells where the DNA will be loaded • DNA samples are mixed with tracking dye which contains sucrose (to weigh down the DNA) and dyes so that you can visualize migration • A buffer containing ions (to conduct an electric current) is placed i ...
FREE Sample Here - We can offer most test bank and
FREE Sample Here - We can offer most test bank and

... and transferred into bacteria, which could then be grown in vats to produce large amounts of the gene’s protein product. This product could be isolated from the bacteria, purified, and then injected into patients to treat hemophilia. Another approach would be to transfer a normal copy of the clottin ...
Mutations Notes
Mutations Notes

... WARM UP “Give what you have. To someone else, it may be better than you dare to think.” –Henry Wordsworth Longfellow 1. What does this quote mean to you? 2. How can you apply this to DNA/RNA/protein structure and function 3. How can you apply this to the long process of discovering DNA and its struc ...
Academic Biology
Academic Biology

... Describe some sex-linked disorders and explain why they are more common in males than in females o o o Male only receives sex-linked alleles from his_________________ o Male needs _____ copy of the sex-linked allele to exhibit the recessive trait o Female must inherit _________recessive alleles – on ...
Chapter 14 – RNA molecules and RNA processing
Chapter 14 – RNA molecules and RNA processing

... different mature mRNA – each results in a different polypeptide ...
Proteins and Nucleic Acids
Proteins and Nucleic Acids

... -purines (double ring) -pyrimidines (single ring) ...
Lecture 21-23
Lecture 21-23

... What is the benefit of having lots of As and Ts here? ii. transcription factors: in eukaryotes, these are proteins that cluster at the promoter. Without them, RNA polymerase won’t bind. More on this in lecture 23. b. RNA polymerase attaches to promoter and separates the two strands of DNA c. begins ...
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CH 2.3-Carbon Compounds

... Chemical Reactions • Processes that change one set of chemicals into another • Involve changes in the chemical bonds that join atoms or compounds ...
Chapter 25 Molecular Basis of Inheritance
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... - James Watson and Francis Crick, determine the structure of DNA and build a model -sit explains how DNA, the genetic material, can vary from species to species and even from individual to individual -discovered the way DNA replicates so that daughter cells and offspring can receive a copy - a polyn ...
Interfering with the genome: A new generation of disease treatments
Interfering with the genome: A new generation of disease treatments

... type of genetic material called RNA. Like DNA, RNA is comprised of nucleic acids, although RNA nucleic acids are subtly different from those of DNA. When a gene is being expressed, the relevant section of the DNA molecule unwinds to expose the underlying code, and RNA nucleic acids then create an in ...
Bioinformatics
Bioinformatics

... • Polymers composed of nucleotide sequences: A adenine, U uracil, guanine G, cytosine C • Single-stranded (RNA) • The RNA molecules are synthesized through a process known as DNA transcription, where a strand of DNA is copied into the corresponding strand of RNA. • There are three common types of RN ...
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3687317_mlbio10_Ch14_TestA_3rd.indd

... 9. People who are heterozygous for sickle cell disease are generally healthy because they a. are resistant to many different diseases. b. have some normal hemoglobin in their red blood cells. c. are not affected by the gene until they are elderly. d. produce more hemoglobin than they need. 10. If no ...
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... • Chromosomes are composed of DNA and protein - the DNA is the genetic material. • Nucleic acid structure gives important insight into genetic function. ...
1 Genetics 301 Sample Second Midterm Examination Solutions
1 Genetics 301 Sample Second Midterm Examination Solutions

... primase- an enzyme which synthesizes short RNA primers which are involved in initiation of DNA replication. peptide bond- bond which joins amino acids in forming a polypeptide chain. wobble pairing- unusual hydrogen bond pairing between bases in the tRNA anticodon and the mRNA codon which allows a s ...
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DNA Replication & Protein Synthesis
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... DNA molecules. • Each new DNA molecule is therefore made up of one strand from the old DNA and a newly synthesized strand of DNA that match up according to the basepair rule. ...
Chapter 13
Chapter 13

... 2. It is made of monomers called nucleotides 3. There are two differences between a DNA & an RNA nucleotide: - RNA has __________________________ instead of deoxyribose - RNA has the base _______________ instead of Thymine - it still has A, C, & G - ____________will pair with __________ (Uracil is a ...
CH2 - SCF Faculty Site Homepage
CH2 - SCF Faculty Site Homepage

... b. A patient in a coma is brought to the emergency room. A blood test shows that he has severe hypoglycemia (abnormally low blood glucose) and acidosis. Treatment is begun immediately to increase both blood sugar and pH. 1) Why is a normal level of blood glucose important? __________________________ ...
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Lecture 4-5 Outline
Lecture 4-5 Outline

... Prokaryotes-initiation of transcription simpler, genes controlled by repressor (turn off) or activator (turn on). Genes occur in groups called "operons", that are transcribed as a single mRNA (turned on together). (Fig. 7-12). Sigma factor (a subunit of the polymerase recognizes the promoter) Eukary ...
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Chapter 12-13 Notes

... Nitrogenous Bases and Covalent Bonds DNA has four kinds of nitrogenous bases: adenine (A), guanine (G), cytosine (C), and thymine (T). ...
Laboratory Exam I - HCC Learning Web
Laboratory Exam I - HCC Learning Web

... Which organelle in the eukaryotic cell contains chromosomal DNA? What type of bond holds 2 polynucleotide (DNA) strands together? What are alternate forms of a gene called? Know how to apply Chargaff’s rules in calculating the percent ratios of aromatic bases in the DNA of a species. Understand depe ...
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Nucleic acid analogue



Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.
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