Presentation
... What is the functional significance of the structural differences between bacterial and eukaryotic mRNAs? ...
... What is the functional significance of the structural differences between bacterial and eukaryotic mRNAs? ...
Searching for Discriminant Fragments of
... Abstract: We collected more than 250 sequences of cytochrome c oxidase for species of the most orders of Hexapoda from Swiss-Prot protein knowledgebase. The discriminant fragments of cytochrome c oxidase at the order level were tentatively determined. We compared the frequency distributions of diffe ...
... Abstract: We collected more than 250 sequences of cytochrome c oxidase for species of the most orders of Hexapoda from Swiss-Prot protein knowledgebase. The discriminant fragments of cytochrome c oxidase at the order level were tentatively determined. We compared the frequency distributions of diffe ...
introacidbase
... • Special case of dipole dipole interaction – Hydrogen covalently attached to O, N, F, or Cl sticks to an unshared pair of electrons on another molecule • H-bond donors – Have the hydrogen ...
... • Special case of dipole dipole interaction – Hydrogen covalently attached to O, N, F, or Cl sticks to an unshared pair of electrons on another molecule • H-bond donors – Have the hydrogen ...
Genetic Engineering
... genes or adding new ones Involves the use of Recombinant DNA (DNA that contains genes from more than one organism) Can be added from the same species or different ones ex. Scientists are trying to insert a gene from cold water flounder into tomato plants to help them resist frost. ...
... genes or adding new ones Involves the use of Recombinant DNA (DNA that contains genes from more than one organism) Can be added from the same species or different ones ex. Scientists are trying to insert a gene from cold water flounder into tomato plants to help them resist frost. ...
Forensic DNA Analysis
... Single-cell sensitivity because each cell contains ~1000 mitochondria = very high contamination risk! Heteroplasmy - more than one mtDNA type manifesting in different tissues in the same individual Lower power of discrimination - maternal relatives all share the same mtDNA ...
... Single-cell sensitivity because each cell contains ~1000 mitochondria = very high contamination risk! Heteroplasmy - more than one mtDNA type manifesting in different tissues in the same individual Lower power of discrimination - maternal relatives all share the same mtDNA ...
Water, Ph, and Macromolecules PPT
... • The pH scale is used to determine if a solution is an acid or a base. – Acids are found below 7. They get stronger as you approach zero. – Neutral substances (like pure water) can be found exactly at 7. – Bases are found above 7. They get stronger as you approach 14. ...
... • The pH scale is used to determine if a solution is an acid or a base. – Acids are found below 7. They get stronger as you approach zero. – Neutral substances (like pure water) can be found exactly at 7. – Bases are found above 7. They get stronger as you approach 14. ...
Compare the activities of the enzymes in prokaryotic transcription to
... 1. Compare the activities of the enzymes in prokaryotic transcription to those in Eukaryotic transcription. ...
... 1. Compare the activities of the enzymes in prokaryotic transcription to those in Eukaryotic transcription. ...
Modern Identification Methods of Bacteria.
... Polymerization: elongation takes place by the presence of enzyme called Taq polymerase (it is derived from an organism called Thermus aquaticus an archea can, hence the name) .So this process takes place at high temperature, 72 degree C. Amplification: the cycle is repeated a number of times, each c ...
... Polymerization: elongation takes place by the presence of enzyme called Taq polymerase (it is derived from an organism called Thermus aquaticus an archea can, hence the name) .So this process takes place at high temperature, 72 degree C. Amplification: the cycle is repeated a number of times, each c ...
Exam #2 Bio310 Microbiology F`06 11/15/06
... Attach reverse transcriptase or integrase, DNA viruses always lack the first and often the second enzyme as well. ...
... Attach reverse transcriptase or integrase, DNA viruses always lack the first and often the second enzyme as well. ...
N & V
... physicochemical properties of these systems can help our understanding of defect repair and drug development. In this study by Jin and coworkers, α-hemolysin protein channels were used as detectors of DNA lesions. The diameter of the narrowest constriction of α-hemolysin was 1.5 nm wide, just narrow ...
... physicochemical properties of these systems can help our understanding of defect repair and drug development. In this study by Jin and coworkers, α-hemolysin protein channels were used as detectors of DNA lesions. The diameter of the narrowest constriction of α-hemolysin was 1.5 nm wide, just narrow ...
DNA - BiVDA
... are faithfully duplicated. The DNA strands are unwound and each parental strand is used as a template in the synthesis of a complementary strand. The new and old strands are then reformed into a tightly wound helix. Although the replication process has high fidelity, errors do occur at very low freq ...
... are faithfully duplicated. The DNA strands are unwound and each parental strand is used as a template in the synthesis of a complementary strand. The new and old strands are then reformed into a tightly wound helix. Although the replication process has high fidelity, errors do occur at very low freq ...
Genetic Engineering
... THE BASICS (CONT.) • Recombinant DNA: DNA that has been artificially made, using DNA from different sources ...
... THE BASICS (CONT.) • Recombinant DNA: DNA that has been artificially made, using DNA from different sources ...
Biochemistry Objective Sheet Test Objectives Bio.1.2.1 • Explain
... polysaccharide, starch, cellulose, glycogen, lipid, glycerol, fatty acid, saturated, unsaturated, phospholipid, steroid, nucleic acid, nucleotide, DNA, RNA, protein, amino acid, R-group, hemoglobin, insulin, enzyme, denature, catalyst, activation energy, substrate, active site, re-usable, specific, ...
... polysaccharide, starch, cellulose, glycogen, lipid, glycerol, fatty acid, saturated, unsaturated, phospholipid, steroid, nucleic acid, nucleotide, DNA, RNA, protein, amino acid, R-group, hemoglobin, insulin, enzyme, denature, catalyst, activation energy, substrate, active site, re-usable, specific, ...
DNA Replication - Texas Tech University
... Regulatory proteins bind to nascent transcript Stabilize RNA to allow mRNA maturation ...
... Regulatory proteins bind to nascent transcript Stabilize RNA to allow mRNA maturation ...
QPCR Helpful Hints
... Primers should be designed to amplify about a 100 bp sequence. The Nelson Lab typically uses the SciTools on the Integrated DNA Technologies (IDT) website (www.idtdna.com) for primer design. ...
... Primers should be designed to amplify about a 100 bp sequence. The Nelson Lab typically uses the SciTools on the Integrated DNA Technologies (IDT) website (www.idtdna.com) for primer design. ...
Genetics BIOL 335 Optional Worksheet 1 solutions 1
... Assume that the bottom strand is the template strand used by RNA polymerase. c. What is the transcribed RNA sequence? If the bottom strand is the template, that means the transcribed RNA is complementary to it. Since polymerases synthesize in a 5'->3' direction, this means the polymerase is moving t ...
... Assume that the bottom strand is the template strand used by RNA polymerase. c. What is the transcribed RNA sequence? If the bottom strand is the template, that means the transcribed RNA is complementary to it. Since polymerases synthesize in a 5'->3' direction, this means the polymerase is moving t ...
Designer Genes - Heredity
... Ribose instead of Deoxyribose Uracil instead of Thymine Messenger RNA – carries blueprint Transfer RNA – brings amino acids Ribosomal RNA – reads code ...
... Ribose instead of Deoxyribose Uracil instead of Thymine Messenger RNA – carries blueprint Transfer RNA – brings amino acids Ribosomal RNA – reads code ...
DNA extraction PRESENTAION
... The isolation of the DNA from biological sample is an essential step in the DNA technology (PCR RFLP- cloning - hyberdization all this approaches require DNA as template • disease diagnosis • DNA sequencing • genetically modified organisms (GMO) - agriculture, pharmaceutical ...
... The isolation of the DNA from biological sample is an essential step in the DNA technology (PCR RFLP- cloning - hyberdization all this approaches require DNA as template • disease diagnosis • DNA sequencing • genetically modified organisms (GMO) - agriculture, pharmaceutical ...
Extracting and Isolating Your Own DNA
... 1) The length of DNA in a cell is about __________________ times as long as the cell itself, yet it is packaged into the tiny nucleus, which takes up only about _____% of the cells total volume. 2) To fit all of this information into the nucleus of a tiny cell, the long strands of DNA are coiled tig ...
... 1) The length of DNA in a cell is about __________________ times as long as the cell itself, yet it is packaged into the tiny nucleus, which takes up only about _____% of the cells total volume. 2) To fit all of this information into the nucleus of a tiny cell, the long strands of DNA are coiled tig ...
Cells
... and associated proteins. During normal cell functions, chromosomes exist as single-stranded structures. During cell division, chromosomes consist of two strands of DNA joined at the centromere. Since the DNA molecules have replicated, one strand of a chromosome is an exact copy of the ...
... and associated proteins. During normal cell functions, chromosomes exist as single-stranded structures. During cell division, chromosomes consist of two strands of DNA joined at the centromere. Since the DNA molecules have replicated, one strand of a chromosome is an exact copy of the ...
Nucleic Acid Biotechnology Techniques
... growing bacterial colonies contain the plasmid of interest ...
... growing bacterial colonies contain the plasmid of interest ...
Slide 1
... Requires the preparation of “competent” host cells Inefficient for generating genomic libraries as overlapping regions needed to place in proper sequence Preference for smaller clones to be transformed If it is an expression vector there are often limitations regarding eukaryotic protein expression ...
... Requires the preparation of “competent” host cells Inefficient for generating genomic libraries as overlapping regions needed to place in proper sequence Preference for smaller clones to be transformed If it is an expression vector there are often limitations regarding eukaryotic protein expression ...
Nucleic acid analogue
Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.