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Poster
Poster

... and topoisomerase I) leaving the DNA free from both of them. The DNA is then electrophoresed on 1.2% agarose at 80 volts for 10 hours at 4°C. The gel is stained with ethidium bromide and photographed (See photo above). The lanes in the gel going from left to right have an increasing amount of histon ...
Quick Look - Strategies for Attaching Oligonucleotides to Solid
Quick Look - Strategies for Attaching Oligonucleotides to Solid

... linker arm) at the time of synthesis using AcryditeTM, an acrylic-phosphoramidite developed by Mosaic Technologies. The AcryditeTM chemistry is stable prior to coupling and will remain stable in aqueous solutions over a wide range of temperature and pH. In addition, it is versatile and can be immob ...
Genetics final exam honors 2010
Genetics final exam honors 2010

... Learning Target #1: Know vocabulary that builds the framework for understanding genetics. Write the best vocabulary word next to the definition that describes it. ______________________________ 1. A segment of DNA; the set of information that controls as trait. ______________________________ 2. An o ...
MNV-VPg-eIF4G-paper.SuppInfo.v2 07/08/2015 A conserved
MNV-VPg-eIF4G-paper.SuppInfo.v2 07/08/2015 A conserved

Name Period ______ Date Chem/Biochem Test Study Guide
Name Period ______ Date Chem/Biochem Test Study Guide

... 29. Briefly describe what happens in each structure of protein folding. a. Primary – Chain of amino acids by peptide bonds. b. Secondary – Hydrogen bonding and formation of alpha helix and beta pleated sheets. c. Tertiary – Hydrophobic and hydrophilic interactions make the protein 3D. d. Quaternary ...
Biological Molecules
Biological Molecules

... – synthesis of biomacromolecules through the condensation reaction – lipids and their sub-units; the role of lipids in the plasma membrane – examples of polysaccharides and their glucose monomer – structure and function of DNA and RNA, their monomers, and complementary base pairing - the nature of t ...
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GD Reagent (Genomic DNA Isolation Reagent)

Chapter 28: Chromosomes
Chapter 28: Chromosomes

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6.G Meiosis Graphic Organizer 6.H Genetic Variation

LS ch 22 part 2 test - Saint Joseph High School
LS ch 22 part 2 test - Saint Joseph High School

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Units_of_Study

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... themselves. In both prokaryotes and eukaryotes the two main types of non-coding RNA are: Ribosomal RNAs (rRNAs), which are the most abundant RNAs in the cell, making up over 80% of the total in actively dividing bacteria. These molecules are components of ribosomes, the structures on which protein s ...
DNA Code problerm
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... C. The entire molecule has encoded information for protein synthesis. D. In the condensed form, it is transcriptionally inactive. E. It must be unpackaged before it can be transcribed into RNA. ...
The Ideal Suppository Base
The Ideal Suppository Base

... the number of mgs of KOH required to neutralize the free acid in 1g of substance is expressed by this value. Low acid value or complete absence of acid are important for good suppository base. Free acids complicate formulation work because they react with other ingredients and can also cause irritat ...
Camp 1 - UCSC Directory of individual web sites
Camp 1 - UCSC Directory of individual web sites

... Each tRNA is specific for only one amino acid (e.g. UAC  Met). Cell carries at least 20 specific enzymes (e.g. AARS) each specific for one amino acid. (i.e. links Amino acids with spec. tRNA) ...
Mandatory additional information
Mandatory additional information

... transgenic mice, phage display library) (for each chain, if different); CDR‐IMGT (e.g., VH  [8.7.11], V‐KAPPA [12.3.9]) and the closer genomic (human or other species) V, J and C  genes and alleles.  2) Name/structure of the antigen against which the monoclonal antibody is directed  3) Laboratory co ...
CHEM 470 - Department of Chemistry and Biochemistry
CHEM 470 - Department of Chemistry and Biochemistry

... Make-up exams: No individual make-up exams will be offered. Your final grade, however, will be based only on the three highest scores; that is, if you miss one exam or score poorly in one of the four hourly exams, this grade will be dropped. Grades: Letter grades will be based on the total points ob ...
Selective Breeding and Genetic Engineering
Selective Breeding and Genetic Engineering

...  Recombinant DNA: Creating DNA molecules (plasmids) with portions from more than one organism  Polymerase Chain Reaction (PCR): Creating multiple copies of a short segment of DNA in a test tube ...
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... Gene expression is regulated by proteins that bind to specific base sequences in DNA. The environment of a cell and of an organism has an impact on gene expression. The promoter as an example of non-coding DNA with a function. Analysis of changes in the DNA methylation patterns. ...
Gene Expression
Gene Expression

... codon AUG in the mRNA. • CAC … V tRNA will bind with the codon GUG in the mRNA. ...
BOTANY-II (wef 2013-14)
BOTANY-II (wef 2013-14)

... Principles of Biotechnology-Construction of the first artificial recombinant DNA molecule, Tools of Recombinant DNA Technology-Restriction Enzymes, Cloning Vectors, Competent Host (For Transformation with Recombinant DNA), Processes of Recombinant DNA Technology- Isolation of the Genetic Material (D ...
Gene Cloning Technology
Gene Cloning Technology

... foreign DNA will be replicated along with the vector DNA   The most commonly used type of cloning vector is a small (4000 bp) circular DNA molecule called a plasmid   Plasmids are found naturally in various types of bacteria   Plasmids can replicate independently of the bacterial chromosome becau ...
Gene Cloning Technology
Gene Cloning Technology

... foreign DNA will be replicated along with the vector DNA   The most commonly used type of cloning vector is a small (4000 bp) circular DNA molecule called a plasmid   Plasmids are found naturally in various types of bacteria   Plasmids can replicate independently of the bacterial chromosome becau ...
Who wants to be a Physiology Millionaire?
Who wants to be a Physiology Millionaire?

... C – gain electrons D – gain protons ...
Chapter 10, 11, 12, 13 Review Questions
Chapter 10, 11, 12, 13 Review Questions

... showing the cross of a purple people eater that is hybrid for horns with a purple people eater that does not have horns. Summarize the genotypes & phenotypes of the possible offspring. 50% chance that the baby will have one horn 11. In dogs, there is a hereditary deafness caused by a recessive gene, ...
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Nucleic acid analogue



Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.
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