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mb_ch10
mb_ch10

... helping Watson and Crick discover DNA’s double helix structure. • Describe the three parts of a nucleotide. • Summarize the role of covalent and hydrogen bonds in the structure of DNA. • Relate the role of the base-pairing rules to the structure of DNA. ...
Mutations Worksheet
Mutations Worksheet

... Complete the boxes below. Classify each as either Frameshift or Point mutations, then specify further with Deletion, Insertion, or Substitution. Use the chart on the following page in order to determine the amino acid sequence. Remember, RNA has Uracil instead of Thymine. ...
Design Genes with Ease Using In-Fusion® Cloning
Design Genes with Ease Using In-Fusion® Cloning

... of poxvirus DNA polymerase (2–4). When incubated with linear duplex DNAs with identical ends in the presence of Mg2+ and low concentrations of dNTP, the 3'–5' proofreading activity of poxvirus DNA polymerase progressively removes nucleotides from the 3' end. This exposes complementary regions on the ...
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File

... Functional groups: a group of atoms that determines how an molecule _______ __________ with other molecules Cause molecules to become electrically charged or polarized, increasing their _________ ...
Gene Expression
Gene Expression

... Tissue-specific transcription factors activate transcription in specific cell types, or in response to specific signals. They bind to short DNA sequences that are near the promoter. – Used to be thought promoters were upstream from the promoter, but it is now known they can be either upstream or dow ...
DNA your onions? - ncbe.reading.ac.uk
DNA your onions? - ncbe.reading.ac.uk

... with heating, degrades the histones associated with the DNA by destroying their secondary and tertiary structures. This allows a protease to hydrolyse the histones to peptides and amino acids. In research, Proteinase K (a protease obtained from the fungus Engyodontium album) is often used to hydroly ...
Overview of the Recombinant DNA technology- the plasmid vector pUC19
Overview of the Recombinant DNA technology- the plasmid vector pUC19

... results in the absence of an EcoR1 site in one of the chromosomes. Upon digestion with EcoR1, the chromosome without the extra EcoR1 site produces a larger fragment than the other one. This difference is recognised using a probe that hybridises within the region encompassed by two flanking EcoR1 sit ...
Multi-Volume Analysis of Nucleic Acids Using the Epoch
Multi-Volume Analysis of Nucleic Acids Using the Epoch

... amounts of nucleic acid template (i.e. the portion of the isolated DNA or RNA that is to be amplified and analyzed) and other reagents, such as enzymes and free nucleotides used for amplification (i.e. in PCR or RT-PCR), are suitable for efficient reactions. ...
As Powerpoint Slide
As Powerpoint Slide

... Figure 2. Modular organization of p53 orthologs of different vertebrate and invertebrate species . TAD, violet: Transactivation Domain; PRD, green: Proline Rich Domain; DBD, yellow: DNA Binding Domain, TD, dark blue: Tetramerization domain; CRD, fuchsia: C-terminal Regulatory Domain. Post-translatio ...
Blank Jeopardy - Hazlet Township Public Schools
Blank Jeopardy - Hazlet Township Public Schools

... A hybrid has two different alleles or genes for a trait. A purebred has two of the same allele or gene that governs a trait. ...
Transposition and transposable elements
Transposition and transposable elements

... • comprise 45% of human chromosomal DNA “middle repetitive DNA” • contribute to spontaneous mutation, genetic rearrangements, horizontal transfer of genetic material • aid speciation and genomic change (in bacteria transposons are often associated with antibiotic resistance genes) • cells must depre ...
AP Molecular Genetics
AP Molecular Genetics

... “It has not escaped our notice that the specific pairing we have postulated immediately suggests a possible copying mechanism for the genetic material.” Watson & Crick ...
Supplementary Material Genomic DNA isolation and bisulfite
Supplementary Material Genomic DNA isolation and bisulfite

... media was then removed from the inserts and replaced with fresh 0.5 mLs of serum-free containing resuspended cells (104 cells). To the lower wells of the plate, 0.75 mLs of Complete Tu medium containing 2% FBS was added. WM1552C/375 cells were additionally transfected with the mIRIDIAN Hairpin Inhib ...
2.3: Carbon-Based Molecules
2.3: Carbon-Based Molecules

... Lock and Key Model Illustrates how enzymes function ...
What minerals in trident gum make your mouth clean?
What minerals in trident gum make your mouth clean?

...  It only has 5 carbon atoms instead of 6 like Sorbitol and Aspartame  Tastes like sugar  CLEANS ...
BIL 250 - Spring 2011 Krempels EXAM III Choose the BEST answer
BIL 250 - Spring 2011 Krempels EXAM III Choose the BEST answer

... d. labeled ribosomes with an appropriate Shine-Dalgarno sequence e. restriction sequences with “sticky ends” matching the enzyme’s amino acid sequence 10. You have a restriction endonuclease that cleaves the phosphodiester bond between two adjacent thymine nucleotides, but only when they occur in th ...
Datasheet for T4 RNA Ligase 1 (ssRNA Ligase), High Concentration
Datasheet for T4 RNA Ligase 1 (ssRNA Ligase), High Concentration

From DNA to Protein: Transcription and Translation
From DNA to Protein: Transcription and Translation

... Both mRNA and tRNA are made of chains of nucleotides • tRNA’s are folded and have an attached amino acid. Both are involved in translation to produce a protein. ...
Timeline of Genetic Engineering
Timeline of Genetic Engineering

...  It also identified about 3 million locations where single-base DNA differences occur in humans. ...
Maximizing Mycobacterium tuberculosis DNA yield for molecular
Maximizing Mycobacterium tuberculosis DNA yield for molecular

... for drug-susceptibility testing and sequencing for epidemiological research. These technologies promise more rapid diagnosis and faster drug-susceptibility profiling. While molecular technologies are being adopted more widely, they have not yet been able to match the sensitivity of culture testing i ...
Abstract
Abstract

Unit 6B Learning Targets
Unit 6B Learning Targets

DNA TEST, PART 2: DNA MESSAGE DECODING You will be given
DNA TEST, PART 2: DNA MESSAGE DECODING You will be given

... FIRST: Put your name, seat number, date, and period at top of page. SECOND: copy the number of your message and the DNA message itself in the spaces so designated. THIRD: decode the message, showing each step completely, just as it happens in your cells; be sure to label each step with the type of m ...
Chapter 4 Genetics: The Science of Heredity
Chapter 4 Genetics: The Science of Heredity

... 5. A number that describes how likely it is that an event will occur. 6. The process that occurs in the formation of sex cells (sperm and egg) by which the number of chromosomes is reduced by half. 8. A characteristic that an organism can pass on to its offspring through its genes. 9. The joining of ...
A Simply Fruity DNA Extraction
A Simply Fruity DNA Extraction

... Grade  7  Standard  Set  2.  Genetics.   A  typical  cell  of  any  organism  contains  genetic  instructions  that  specify  its  traits.  Those  traits  may  be   modified  by  environmental  influences.  As  a  basis  for  understandin ...
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Nucleic acid analogue



Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.
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