Biol 207 Dr. Locke`s section WS9 Page 1 Workshop 9 Biol207
... pair recognition sequence, what is the average E. coli genomic DNA fragment size expected based solely on chance (assume equal frequencies of A, C, G, and T)? g) Using your answer from part “F”, and if the E.coli genome is 4.5 Mbp, how many clones would you need to test to make sure you had 99% prob ...
... pair recognition sequence, what is the average E. coli genomic DNA fragment size expected based solely on chance (assume equal frequencies of A, C, G, and T)? g) Using your answer from part “F”, and if the E.coli genome is 4.5 Mbp, how many clones would you need to test to make sure you had 99% prob ...
Genetic Engineering
... and stability with the aim of adapting it for largefragment cloning. A group led by Melvin Simon modified an endogenous circular plasmid in E. coli, the fertility (F) factor present at one or two copies per cell, to create a cloning vector. In reference to its yeast cousin, they called it bacterial ...
... and stability with the aim of adapting it for largefragment cloning. A group led by Melvin Simon modified an endogenous circular plasmid in E. coli, the fertility (F) factor present at one or two copies per cell, to create a cloning vector. In reference to its yeast cousin, they called it bacterial ...
C - Eric Hamber Secondary
... - heavy metals (mercury, lead etc.) bind preferentially with specific R group bonds (the S in Cystine), breaking the tertiary structure. C11. FUNCTIONS OF PROTEINS - polymers of amino acids - have 2 major functions I) Structural - large proteins are important - muscle, tendon, cartilage, hair etc. K ...
... - heavy metals (mercury, lead etc.) bind preferentially with specific R group bonds (the S in Cystine), breaking the tertiary structure. C11. FUNCTIONS OF PROTEINS - polymers of amino acids - have 2 major functions I) Structural - large proteins are important - muscle, tendon, cartilage, hair etc. K ...
BIOLOGICAL MOLECULES
... Selective breeding is where desirable characteristics in either plants or animals are increased by only breeding with organisms which show those traits. Examples include: Plants (give one): ...
... Selective breeding is where desirable characteristics in either plants or animals are increased by only breeding with organisms which show those traits. Examples include: Plants (give one): ...
Biology Midterm Review Sheet
... large, complex sequences of amino acids) and RNA. B. GENE STRUCTURE - TWO TYPES OF NUCLEIC ACIDS: 1. Deoxyribonucleic acid (DNA) 2. Ribonucleic acid (RNA) C. NUCLEOTIDES: The monomers of nucleic acids - three subunits: 3. Sugar (deoxyribose in DNA; ribose in RNA) 4. Phosphate 5. Nitrogenous base (5 ...
... large, complex sequences of amino acids) and RNA. B. GENE STRUCTURE - TWO TYPES OF NUCLEIC ACIDS: 1. Deoxyribonucleic acid (DNA) 2. Ribonucleic acid (RNA) C. NUCLEOTIDES: The monomers of nucleic acids - three subunits: 3. Sugar (deoxyribose in DNA; ribose in RNA) 4. Phosphate 5. Nitrogenous base (5 ...
Chapter 20
... genes & organisms, then you need a set of tools to work with this unit is a survey of those tools… ...
... genes & organisms, then you need a set of tools to work with this unit is a survey of those tools… ...
CST Review Study Guide Biochemistry (Unit 2) 1. What elements
... 34. The gene for color vision (C) is dominant to the gene for color blindness (c) and is located on the X chromosome. If a color blind man and a woman with homozygous normal color vision have children, what are the chances that they will have a colorblind child? 35. Why do some lethal (deadly) allel ...
... 34. The gene for color vision (C) is dominant to the gene for color blindness (c) and is located on the X chromosome. If a color blind man and a woman with homozygous normal color vision have children, what are the chances that they will have a colorblind child? 35. Why do some lethal (deadly) allel ...
2421_Ch2.ppt
... fit in a lock-and-key fashion. Thus, enzymes are specific and generally only work with their own specialized reactions. Enzymes typically reduce the activation energy so less energy is required to run the reaction. This makes the reaction far more efficient ...
... fit in a lock-and-key fashion. Thus, enzymes are specific and generally only work with their own specialized reactions. Enzymes typically reduce the activation energy so less energy is required to run the reaction. This makes the reaction far more efficient ...
lec03-1
... 6. Promoters for RNA polymerase (1). Short sequences at -30, -75, -90 -basal element. (2). TATA box is a crucial positioning component of the core promoter. (3). The CAAT box (-75) determines the efficiency of the promoter a. The CAAT box can interact with CTF and the factors CP1 and CP2 (gene spec ...
... 6. Promoters for RNA polymerase (1). Short sequences at -30, -75, -90 -basal element. (2). TATA box is a crucial positioning component of the core promoter. (3). The CAAT box (-75) determines the efficiency of the promoter a. The CAAT box can interact with CTF and the factors CP1 and CP2 (gene spec ...
doc
... resuspension must be achieved in order to succeed in the isolation of the plasmid! 8. Incubate 5 minutes at room temperature. 9. Add 400 l of your freshly made NaOH 0.2M SDS 1 % solution and mix by hand. 10. Leave this for 5 to 10 minutes on crushed ice until you have a clear solution. 11. Add 300 ...
... resuspension must be achieved in order to succeed in the isolation of the plasmid! 8. Incubate 5 minutes at room temperature. 9. Add 400 l of your freshly made NaOH 0.2M SDS 1 % solution and mix by hand. 10. Leave this for 5 to 10 minutes on crushed ice until you have a clear solution. 11. Add 300 ...
Laboratory Protocols
... resuspension must be achieved in order to succeed in the isolation of the plasmid! 8. Incubate 5 minutes at room temperature. 9. Add 400 µl of your freshly made NaOH 0.2M SDS 1 % solution and mix by hand. 10. Leave this for 5 to 10 minutes on crushed ice until you have a clear solution. 11. Add 300 ...
... resuspension must be achieved in order to succeed in the isolation of the plasmid! 8. Incubate 5 minutes at room temperature. 9. Add 400 µl of your freshly made NaOH 0.2M SDS 1 % solution and mix by hand. 10. Leave this for 5 to 10 minutes on crushed ice until you have a clear solution. 11. Add 300 ...
Lines of Evidence for Evolution
... HIV is the retrovirus that causes acquired immune deficiency syndrome (AIDS) in humans. As a retrovirus, the virus particle (called a virion) contains RNA not DNA. When a retrovirus infects a host cell (1), the RNA becomes translated into DNA (2-3) by a protein called "reverse transcriptase" that is ...
... HIV is the retrovirus that causes acquired immune deficiency syndrome (AIDS) in humans. As a retrovirus, the virus particle (called a virion) contains RNA not DNA. When a retrovirus infects a host cell (1), the RNA becomes translated into DNA (2-3) by a protein called "reverse transcriptase" that is ...
A comparison of DNA quantification values obtained by
... obtained using the NanoDrop were more variable, as indicated by the higher maximum sample SD value. DNA concentration values determined by UV spectrophotometry were significantly higher than those determined by PicoGreen®. Of the two UV spectrophotometry-based instruments, the values obtained using ...
... obtained using the NanoDrop were more variable, as indicated by the higher maximum sample SD value. DNA concentration values determined by UV spectrophotometry were significantly higher than those determined by PicoGreen®. Of the two UV spectrophotometry-based instruments, the values obtained using ...
Document
... is involved in the resolution of concatemeri cgenomes during the phage assembly process. This enzyme is a heteromeric protein possessing several activities including DNA binding, nicking of the DNA at the cos site and dissociationof the cohesive ends. ATP serves as a requiredcofactor for l-Terminase ...
... is involved in the resolution of concatemeri cgenomes during the phage assembly process. This enzyme is a heteromeric protein possessing several activities including DNA binding, nicking of the DNA at the cos site and dissociationof the cohesive ends. ATP serves as a requiredcofactor for l-Terminase ...
ch11dna - cpolumbo
... Double helix—two coiled DNA strands Composed of nucleotides—units containing a sugar molecule (deoxyribose), phosphate group and a nitrogen-containing base In humans, the order of these bases is 99.9% the same. Four bases Adenine Cytosine Guanine Thymine Bases always pair A to T an ...
... Double helix—two coiled DNA strands Composed of nucleotides—units containing a sugar molecule (deoxyribose), phosphate group and a nitrogen-containing base In humans, the order of these bases is 99.9% the same. Four bases Adenine Cytosine Guanine Thymine Bases always pair A to T an ...
Cdc45: the missing RecJ ortholog in eukaryotes?
... The ribbon diagram was drawn using the Pymol protein structure visualization program (http://www.pymol.org/). (C) HMMer and HHpred comparison E-values between Cdc45 and RecJ families. Numbers correspond to HMMer profile versus sequence (gray boxes) and HHpred profile versus profile (blue box) compar ...
... The ribbon diagram was drawn using the Pymol protein structure visualization program (http://www.pymol.org/). (C) HMMer and HHpred comparison E-values between Cdc45 and RecJ families. Numbers correspond to HMMer profile versus sequence (gray boxes) and HHpred profile versus profile (blue box) compar ...
I ADDED TISSUES JUST IN CASE!!! APHY 101, Lecture 4
... Obtains energy from Transport Chain Complexes ...
... Obtains energy from Transport Chain Complexes ...
Organization: The 6 Essential Elements
... There are several types of lipids, but all contain subunits of glycerol and fatty acids made of carbon, hydrogen, and oxygen. It is different from a carbohydrate because of the ratio and because the smaller units do not link together to form a chemical chain ...
... There are several types of lipids, but all contain subunits of glycerol and fatty acids made of carbon, hydrogen, and oxygen. It is different from a carbohydrate because of the ratio and because the smaller units do not link together to form a chemical chain ...
blank worksheet
... Performed by: Date: Institution: Step 1: Sample Preparation & Nucleic Acid Isolation For great results, use (click product names to learn more): Roche High Pure RNA Isolation Kit Roche High Pure FFPET RNA Isolation Kit Roche High Pure miRNA Isolation Kit Roche RealTime ready Cell Lysis Kit ...
... Performed by: Date: Institution: Step 1: Sample Preparation & Nucleic Acid Isolation For great results, use (click product names to learn more): Roche High Pure RNA Isolation Kit Roche High Pure FFPET RNA Isolation Kit Roche High Pure miRNA Isolation Kit Roche RealTime ready Cell Lysis Kit ...
Homework 4
... 8. Given the following parent strand sequence, what would the daughter strand sequence look like (Hint: refer to figure 8.10 and 8.12? 5´ – G C T A A C T G T G A T C G T A T A A G C T G A – 3´ 9. Describe the process of DNA replication. 10-11. Compare and contrast transcription and translation. 12. ...
... 8. Given the following parent strand sequence, what would the daughter strand sequence look like (Hint: refer to figure 8.10 and 8.12? 5´ – G C T A A C T G T G A T C G T A T A A G C T G A – 3´ 9. Describe the process of DNA replication. 10-11. Compare and contrast transcription and translation. 12. ...
PTC Lab Instructions/Information
... 2. Using what you know about genetics, SNPs, and the PTC gene, explain why it is possible for a person to be a “weak taster.” 3. Some studies have shown that PTC “tasters” are less likely to become smokers. Why do you think scientists are seeing this correlation? 4. How can the techniques described ...
... 2. Using what you know about genetics, SNPs, and the PTC gene, explain why it is possible for a person to be a “weak taster.” 3. Some studies have shown that PTC “tasters” are less likely to become smokers. Why do you think scientists are seeing this correlation? 4. How can the techniques described ...
Nucleic acid analogue
Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.