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PPT presentation - Yavapai College
PPT presentation - Yavapai College

... DNA is transcribed and translated to make proteins that run cell metabolism • DNA is transcribed to mRNA • mRNA is translated to amino acid sequence • Amino acid sequence folds up into protein • Proteins catalyze reactions of cell metabolism • This process is called “gene expression”—the informatio ...
View Poster - Technology Networks
View Poster - Technology Networks

... single sperm cell samples with a mean PCR efficiency of 62.8%. Furthermore, we were able to identify a sample containing more than one cell allowing to exclude “contaminated” samples from further analysis. ...
Major Domains of Life
Major Domains of Life

... Nucleic Acids – Larges macromolecules found. They are collections of individual nucleotides linked into linear polymers. Each nucleotide consists of one sugar, one nitrogeneous base and one or more phosphate groups. Examples: DNA and RNA ...
Comparative Study of DNA Isolated from Increasing
Comparative Study of DNA Isolated from Increasing

classes of mutation
classes of mutation

GP100 Genomic DNA Mini Kit _Plant_ protocol
GP100 Genomic DNA Mini Kit _Plant_ protocol

Expression of a mouse replacement histone H3. 3 gene with a
Expression of a mouse replacement histone H3. 3 gene with a

... EMBL data bases, using the Goad and Kanehisa DNA sequence similarity program (35), revealed about 80% homology of the coding region with mouse, human and chicken histone H3 genes (Table 1) and with all other H3 histone genes registered in the data bases (unpublished results). Furthermore, at the ami ...
to get the file - Chair of Computational Biology
to get the file - Chair of Computational Biology

An Optical Conveyor for Molecules
An Optical Conveyor for Molecules

... Since the conveyor belt is driven entirely optically, its position can be changed easily. Instead of waiting for the molecules to diffuse into the trap, they can be collected while passing over them. Figure 3a shows 40 nm polystyrene beads. Comparable to a vacuum cleaner, the radial conveyor belt co ...
Metabolism of amino acids, porphyrins
Metabolism of amino acids, porphyrins

... •The others are classed as "essential" amino acids and must be obtained in the diet ...
I N IN  VIVO VITRO
I N IN VIVO VITRO

... at least 7 hours after infection, under conditions where synthesis of cellular RNA and protein is inhibited. Although the possibility of some residual protein synthesis sufficient for the formation of a small amount of rirus-induced enz\·me cannot be excluded, the RNA synthesis seems to ·be dependen ...
Next-generation DNA sequencing techniques
Next-generation DNA sequencing techniques

... ‘reversible terminators’ for speed and efficiency [8]. The first step of the technique consisted in detecting the next added fluorescently labelled base (reversible terminator) in the growing DNA chain by means of a sensitive CCD camera. This was performed on a large number of DNA samples in paralle ...
DLBCL PAC Pitch - World CDx Boston 2016
DLBCL PAC Pitch - World CDx Boston 2016

... © 2012 NanoString® Technologies, Inc. All rights reserved. ...
Compaction of Duplex Nucleic Acids upon Native
Compaction of Duplex Nucleic Acids upon Native

DNA polymerase active site is highly mutable
DNA polymerase active site is highly mutable

... extent of the conformational change of the finger motif during the dNTP binding step (Fig. 1), and is replaced only by amino acids that can fit into this pocket. Glu-615 hydrogen bonds with Tyr-671 (a residue located in helix O within the finger motif and stacks with the base portion of the incoming ...
Metabolism II
Metabolism II

... An amino acid may be synthesized if there is an available "root" ketoacid with a synthetic connection to the final amino acid. Since an appropriate "root" keto acid does not exist for eight amino acids, (lys, leu, ile, met, thr, try, val, phe), they are essential and must be included in the diet bec ...
DNA Profiling
DNA Profiling

... • DNA is wound around proteins (histones) and condensed during cell division into chromosomes ...
Translation Notes
Translation Notes

... –Long chain of many amino acids –Proteins differ in the number and order of amino acids. ...
CHAPTER 3 THE CHEMISTRY OF LIFE Section 1: Matter and
CHAPTER 3 THE CHEMISTRY OF LIFE Section 1: Matter and

Biochemistry 462a - Proteins: Primary Sequence
Biochemistry 462a - Proteins: Primary Sequence

... The amino acid peaks are detected using Ninhydrin, which reacts with the free amino groups of amino acids to produce a purple color. ...
doc - Vanderbilt University
doc - Vanderbilt University

... test tube was amazing. I had no idea that DNA can actually be seen with the naked eye. But it was there, in my little tube exposed by a concoction of chemicals that my shaky pipette had miraculously delivered in the appropriate amount. It was an impossibly thin strand curled and knotted and suspende ...
1 A
1 A

... system is +,167 V. This suggests that in the absence of any complexing agents the Cu2+ ions are more stable. Depending on the type of the ligands either the CuII, or the CuI state can stabilised. From the data in the above Table it can be concluded that the lower oxidation state of copper the CuI c ...
CHAPTER-7 EQUILIBRIUM Equilibrium state- When
CHAPTER-7 EQUILIBRIUM Equilibrium state- When

...  Buffer solution :The solutions which resist change in pH on dilution or with the addition of small amounts of acid or alkali are called Buffer Solutions.  common ion effect: It can be defined as a shift in equilibrium on adding a substance that provides more of an ionic species already present in ...
Acids and Bases The pH Scale
Acids and Bases The pH Scale

... combine with OH", forming water. A base has the opposite effect, increasing OH" concentration but also reducing H! concentration by the formation of water. If enough of a base is added to raise the OH" concentration to 10"4 M, it will cause the H! concentration to drop to 10"10 M. Whenever we know t ...
Contents Introduction Storage and Stability - Omega Bio-tek
Contents Introduction Storage and Stability - Omega Bio-tek

... specially formulated buffer. The protein is precipitated by adding SQ2. After removal of the protein, the supernatant is mixed with 1 volume of isopropanol to precipitate the DNA. The DNA pellet is washed with 70% ethanol and dissolved with water or low ionic strength buffer. Purified DNA can be dir ...
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Nucleic acid analogue



Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.
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