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Chapter 8
Chapter 8

Gluconacetobacter entanii sp. nov., isolated from submerged high
Gluconacetobacter entanii sp. nov., isolated from submerged high

... vinegar fermentations in the Southern part of Germany. Four strains (LTH 4560T, LTH 4341, LTH 4551 and LTH 4637) were characterized in more detail and it was revealed that they have in common certain properties such as requirement of acetic acid, ethanol and glucose for growth, and no overoxidation ...
Deoxyribonucleic Acid Base Compositions and Nucleotide
Deoxyribonucleic Acid Base Compositions and Nucleotide

... NaCl plus 0.015 M trisodium citrate, pH 7) and 0.1 volume of chloroform-i-amylalcohol were added to the DNA solution, and the preparation was shaken for 10 min at 100 rpm. Centrifugation at 27,000 x g for 20 min separated the emulsion into three layers. From the upper layer the DNA threads were prec ...
SUGAR AND ACID  METABOLISM IN  CITRUS  FRUIT 1
SUGAR AND ACID METABOLISM IN CITRUS FRUIT 1

... The possibility of organic acid transport from peel to pulp is also suggested by several observations. The first of these is that the arsenic treatments noted above affect juice acidity without the arsenic ever reaching the pulp. Only leaves and peel retain levels of this compound that can be readil ...
Biosensor - PharmaStreet
Biosensor - PharmaStreet

... 6. Bio-mimetic materials A bio mimetic biosensor is an artificial or synthetic sensor that mimics the function of a natural biosensor . These can include aptasensors , where apta -sensors use aptamers as the biocomponent. Aptamers are synthetic single stranded nucleic acid that can be designed to i ...
1 - Pearson Schools and FE Colleges
1 - Pearson Schools and FE Colleges

Identification of novel sulfur-containing bacterial
Identification of novel sulfur-containing bacterial

... MSM agar plates which contained PTUD, PTO, octylthiohexanoic acid, BTV or propylthiopropionic acid as carbon sources. Reference growth experiments were performed on MSM agar plates which contained only sodium gluconate as sole carbon source. All Pseudomonas strains investigated in this study were ab ...
MD Simulations of the P53 oncoprotein structure
MD Simulations of the P53 oncoprotein structure

Chapter 12 Recombinant DNA Technology Key Concepts
Chapter 12 Recombinant DNA Technology Key Concepts

... the choice between them often depends on the size of the DNA segment that needs to be cloned and on the intended application for the cloned gene. We shall consider several commonly used types. Plasmids. As described earlier, bacterial plasmids are small circular DNA molecules that are not only disti ...
Oscillatory Oxidation of Malonic Acid by Bromate
Oscillatory Oxidation of Malonic Acid by Bromate

... tion products of malonic acid cannot be excluded on the basis of the present data, however, such reac­ tions should not contribute more than about 10 —20% to the overall scheme. The overall reactions given here do not contain formic acid as final product as suggested elsewhere 4. Under the condition ...
Oxypred: Prediction and Classification of Oxygen-Binding
Oxypred: Prediction and Classification of Oxygen-Binding

... Oxygen-binding proteins are widely present in eukaryotes ranging from non-vertebrates to humans (1 ). Moreover, these proteins have also been reported to be present in many prokaryotes and protozoans (2 ). The occurrence of oxygen-binding proteins in all kingdoms of organisms, though not in all orga ...
pdf
pdf

... between a growing polypeptide and an incoming aminoacyl-tRNA. The ribosomes insures that the amino acids are added in the order specified by the mRNA. b. Ribosomes associate reversibly with the mRNA. The two subunits of the ribosome form a complex around the mRNA to translate, and then dissociate af ...
File - PWilsonScience
File - PWilsonScience

Impact of the Protein Data Bank on Drug Development
Impact of the Protein Data Bank on Drug Development

... AnAbioAc Arms Race I: New Drugs  More Penicillin Analogs then Cephalosporins  •  Drug companies targeted DD‐TranspepAdases with  4‐6 bicyclic cephalosporin variaAons on the   4‐5 bicyclic β‐lactam scaffold common to Penicillins  •  Cephalosporins are also D‐Ala‐D‐Ala mimics  ...
Chapter 5
Chapter 5

... Examples of amino acid subunits ...
Plasmids
Plasmids

... 6. Add 350 µL of cold Yellow Zyppy Neutralization buffer (w/RNAase A) to the tube, and mix the contents thoroughly by inverting several times. The solution will turn yellow when neutralization is complete, and a yellowish precipitate will form. Invert the sample an additional 3-4 times to ensure com ...
Pentose Phosphate Pathway
Pentose Phosphate Pathway

... Glyceraldehyde‐3‐P and fructose‐6‐P may be converted to  glucose‐6‐P, via enzymes of gluconeogenesis, for reentry   to Pentose Phosphate Pathway, maximizing formation of  NADPH, which is need for reductive biosynthesis. ...
Ho - Engineering Computing Facility
Ho - Engineering Computing Facility

... DNA polymerases do not have perfect fidelity. The error rate per base duplication,depends on the polymerase and temperatures used. Because the error rate is on a per base duplication basis, the fraction of mutant tags is then dependent on the number of bases within each ditag, N. Errors that are ...
Amplification and partial sequencing of Ixodes Scapularis Shaker
Amplification and partial sequencing of Ixodes Scapularis Shaker

... passing pathogens to humans that cause lyme disease, rocky mountain spotted fever and tularemia (9). The research project undertaken provides an easy and efficient means to begin the tick DNA sequencing on a small scale, which can be done in almost any molecular biology laboratory. The sequence homo ...
Acids, Bases and Salts
Acids, Bases and Salts

... the Brønsted-Lowry theory is an acid-base theory, proposed independently by Danish Johannes Nicolaus Brønsted and English Thomas Martin Lowry in 1923. In this system, an acid is defined as any chemical species (molecule or ion) that is able to lose, or "donate" a hydrogen ion (proton), and a base is ...
Dyeing of Wool, Silk and Acrylic
Dyeing of Wool, Silk and Acrylic

... – They have average substantivity that’s why they have average levelling property. – applied in 5-6 pH, ...
Notification of a Notifiable Low Risk Dealing
Notification of a Notifiable Low Risk Dealing

genetic diversity of american-type vaccine-derived prrs
genetic diversity of american-type vaccine-derived prrs

Biosynthesis of Amino Acids
Biosynthesis of Amino Acids

Next-Generation Sequencing: an overview of technologies and
Next-Generation Sequencing: an overview of technologies and

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Nucleic acid analogue



Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.
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