Protocol: Cloning of oligos for sgRNA (CRISPR) or
... This protocol allows you to clone oligos to generate shRNAs or sgRNAs on a small scale. -For sgRNAs, pXPR vectors with a single BsmBI or BbsI cloning site are most common, two types of pXPR vectors can be used: 1. pXPR_003 (or “lenti guide”) will only contain an sgRNA and is to be used in a cell lin ...
... This protocol allows you to clone oligos to generate shRNAs or sgRNAs on a small scale. -For sgRNAs, pXPR vectors with a single BsmBI or BbsI cloning site are most common, two types of pXPR vectors can be used: 1. pXPR_003 (or “lenti guide”) will only contain an sgRNA and is to be used in a cell lin ...
Organic Chemistry Notes Student
... A. Carbohydrates - ______________________________________ Monosaccharides are the simplest ______________________. • The carbohydrate monomers are ___________________ • A monosaccharide has a formula that is a multiple of CH2O ______________________ ...
... A. Carbohydrates - ______________________________________ Monosaccharides are the simplest ______________________. • The carbohydrate monomers are ___________________ • A monosaccharide has a formula that is a multiple of CH2O ______________________ ...
A General Method for the Determination of the Carboxyl
... shown that without the use of diagonal markers it is not always obvious which peptides lie on the diagonal line. If a particular digestion procedure releases the amino acids lysine, arginine, or more particularly histidine, these can easily be mistaken for carboxyl-terminal peptides (Fig. 1). Diagon ...
... shown that without the use of diagonal markers it is not always obvious which peptides lie on the diagonal line. If a particular digestion procedure releases the amino acids lysine, arginine, or more particularly histidine, these can easily be mistaken for carboxyl-terminal peptides (Fig. 1). Diagon ...
Raven/Johnson Biology 8e Chapter 17 Answers 1. A recombinant
... b. constructed from DNA from different sources c. constructed from novel combinations of DNA from the same source d. produced through mitotic cell division The correct answer is b— A. Answer a is incorrect. Crossing over produces novel combinations of DNA from a single individual. The offspring are ...
... b. constructed from DNA from different sources c. constructed from novel combinations of DNA from the same source d. produced through mitotic cell division The correct answer is b— A. Answer a is incorrect. Crossing over produces novel combinations of DNA from a single individual. The offspring are ...
The Process of How COMT Genetic Variants Provide the
... the DNA code are significant enough to differentiate every human being. The COMT gene is a small portion of a human DNA sequence that provides instructions on how to build the COMT enzyme. The COMT enzyme, a molecule that changes the shape of dopamine, is a critically important protein that regulate ...
... the DNA code are significant enough to differentiate every human being. The COMT gene is a small portion of a human DNA sequence that provides instructions on how to build the COMT enzyme. The COMT enzyme, a molecule that changes the shape of dopamine, is a critically important protein that regulate ...
Hypercholesterolemia
... sample is cooled to a temperature between 42°C and 65°C. This “cooling” allows hybridization of a set of two small (15-30) synthetic oligonucleotides, known as “primers”, to the target region to be amplified. In this experiment, the target is the FH gene. In the third step (extension), the temperatur ...
... sample is cooled to a temperature between 42°C and 65°C. This “cooling” allows hybridization of a set of two small (15-30) synthetic oligonucleotides, known as “primers”, to the target region to be amplified. In this experiment, the target is the FH gene. In the third step (extension), the temperatur ...
Minireview Alpha Satellite and the Quest for the Human Centromere
... to these questions have baffled biologists for over a century. The best-characterized centromere is found in the yeast Saccharomyces cerevisiae. In this unicellular eukaryote, centromere function is conferred by a small (125 bp) DNA sequence whose overall organization and sequence composition are hi ...
... to these questions have baffled biologists for over a century. The best-characterized centromere is found in the yeast Saccharomyces cerevisiae. In this unicellular eukaryote, centromere function is conferred by a small (125 bp) DNA sequence whose overall organization and sequence composition are hi ...
Physiological characterization of natural transformation in
... (Table 2) were about 50-100 times lower than with pAVA2 13-8. This indicates that replacement recombination is more efficient than Campbell-like integration. To test whether single-stranded chromosomal Acinetobacter DNA could give rise to transformants, the chromosomal insert of pAVA213-8, containin ...
... (Table 2) were about 50-100 times lower than with pAVA2 13-8. This indicates that replacement recombination is more efficient than Campbell-like integration. To test whether single-stranded chromosomal Acinetobacter DNA could give rise to transformants, the chromosomal insert of pAVA213-8, containin ...
ISH ISH ISH ISH ISH
... Perfusion is much better at preserving tissue quality and RNA integrity because of the rapid spread of fixative through the cells In addition, perfusion results in ISH data with low background due to clearance of blood cells from the tissue Fixation by immersion, on the other hand, should be used wh ...
... Perfusion is much better at preserving tissue quality and RNA integrity because of the rapid spread of fixative through the cells In addition, perfusion results in ISH data with low background due to clearance of blood cells from the tissue Fixation by immersion, on the other hand, should be used wh ...
NAP57, a Mammalian Nucleolar Protein with a Putative Homolog
... Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York 10461; and * Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, New York 10021 ...
... Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York 10461; and * Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, New York 10021 ...
HILL12_Lecture_16
... of groups that are far apart on the protein chain. One example is the protein chain in globular proteins. © 2010 Pearson Prentice Hall, Inc. ...
... of groups that are far apart on the protein chain. One example is the protein chain in globular proteins. © 2010 Pearson Prentice Hall, Inc. ...
Plant/Fungi Total RNA Purification 96-Well Kit
... Norgen’s Plant/Fungi Total RNA Purification 96-Well Kit provides a rapid method for the highthroughput isolation of total RNA from plants and fungi. The RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform. The purified RNA is of the ...
... Norgen’s Plant/Fungi Total RNA Purification 96-Well Kit provides a rapid method for the highthroughput isolation of total RNA from plants and fungi. The RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform. The purified RNA is of the ...
Translation - Advanced
... folding of the protein into its functional conformation. During and after synthesis, polypeptide chains often fold into secondary and then tertiary structures. These levels of organization were discussed in the Organic Compounds: Proteins (Advanced) concept. Briefly, the primary structure of the pro ...
... folding of the protein into its functional conformation. During and after synthesis, polypeptide chains often fold into secondary and then tertiary structures. These levels of organization were discussed in the Organic Compounds: Proteins (Advanced) concept. Briefly, the primary structure of the pro ...
Thermostable glycerol kinase from a
... of forward (59-ATATACCATGGAAAAGTTCGTTCTTTC39) and reverse (59-AATGGATCCATATCAATTTGATTTTGCACT-39) primers, where the underlines represent the NcoI and BamHI sites, respectively, and the ATG codon for the initiation of the translation and the sequence complementary to the termination codon TGA are sho ...
... of forward (59-ATATACCATGGAAAAGTTCGTTCTTTC39) and reverse (59-AATGGATCCATATCAATTTGATTTTGCACT-39) primers, where the underlines represent the NcoI and BamHI sites, respectively, and the ATG codon for the initiation of the translation and the sequence complementary to the termination codon TGA are sho ...
ppt presentation
... SOLiD - Sequencing by Oligonucleotide Ligation and Detection - ligation of labelled oligonucleotides ...
... SOLiD - Sequencing by Oligonucleotide Ligation and Detection - ligation of labelled oligonucleotides ...
Learning About DNA
... scientists developed a tool to make things look larger than their actual size. Microscopes use lenses (curved pieces of glass) to bend light rays in order to make an enlarged image. The first compound microscope was invented around 1590 by two Dutch eyeglass makers, Hans and Zacharias Janssen. Their ...
... scientists developed a tool to make things look larger than their actual size. Microscopes use lenses (curved pieces of glass) to bend light rays in order to make an enlarged image. The first compound microscope was invented around 1590 by two Dutch eyeglass makers, Hans and Zacharias Janssen. Their ...
Protein synthesis (Primer)
... • Tight turns nearly completely reverse the direction of the peptide chain • Loops often play important functional roles—the active sites of many enzymes and receptors are made of loop residues • Bends in alpha helix allows the helix to “hug” the protein • Bulges in edge beta strands are important n ...
... • Tight turns nearly completely reverse the direction of the peptide chain • Loops often play important functional roles—the active sites of many enzymes and receptors are made of loop residues • Bends in alpha helix allows the helix to “hug” the protein • Bulges in edge beta strands are important n ...
Structure of LEP100, a Glycoprotein That Shuttles between
... amino acid sequence of LEP100, determined by sequential automated Edman degradation and product analysis, was PheAspValArgAspSefrhrGlyLysValCysIleIleAlaXLeuThrValAlaPheSerValGluTyrLysXXGlyGlnLys. Position 15 was deduced to be Asn on the basis of failure to identify any residue with or without reduct ...
... amino acid sequence of LEP100, determined by sequential automated Edman degradation and product analysis, was PheAspValArgAspSefrhrGlyLysValCysIleIleAlaXLeuThrValAlaPheSerValGluTyrLysXXGlyGlnLys. Position 15 was deduced to be Asn on the basis of failure to identify any residue with or without reduct ...
better samples for better results
... • Stainless/label-free and nondestructive so samples can be re-used for downstream analysis. • Quantitative assessment of tissue/cell type that removes subjectivity. • Automated analysis for high throughput screening. Extensive literature exists demonstrating the unique capability of FTIR imaging ...
... • Stainless/label-free and nondestructive so samples can be re-used for downstream analysis. • Quantitative assessment of tissue/cell type that removes subjectivity. • Automated analysis for high throughput screening. Extensive literature exists demonstrating the unique capability of FTIR imaging ...
Evaluation of volatile compounds produced by Lactobacillus
... The vessels were hermetically sealed with an aluminum seal and Butyl–Teflon septa. During sampling of volatile compounds, the vials were thermostatized at 40±1 °C for 10 min, and then CAR/PDMS 75 μm (Supelco Inc. Bellefonte, PA, USA) fiber was exposed into the headspace for 30 min. Chromatographic a ...
... The vessels were hermetically sealed with an aluminum seal and Butyl–Teflon septa. During sampling of volatile compounds, the vials were thermostatized at 40±1 °C for 10 min, and then CAR/PDMS 75 μm (Supelco Inc. Bellefonte, PA, USA) fiber was exposed into the headspace for 30 min. Chromatographic a ...
Protein Catabolism
... rings are called the β-subunits. The active sites are found in the N-terminal of the β-subunits. These active sites contain a threonine or serine residue that functions as nucleophile to attack the carbonyl bonds of proteins to form acyl-enzyme intermediates which are then hydrolyzed by water. The s ...
... rings are called the β-subunits. The active sites are found in the N-terminal of the β-subunits. These active sites contain a threonine or serine residue that functions as nucleophile to attack the carbonyl bonds of proteins to form acyl-enzyme intermediates which are then hydrolyzed by water. The s ...
Genomic organization of lignin peroxidase genes of Phanerochaete
... would differentiate LiPA (2353 bp) from its' allele LPOA (2573 bp), and it would also hybridize to LiPB (1475 bp) and its' allele LPOB (1593 bp). The 28-mer hybridizes to a position 600 bp 3' of LiPA's translational stop codon. This region of intergenic LiPB-LiPA shows no homology to LPOB-LPOA (see ...
... would differentiate LiPA (2353 bp) from its' allele LPOA (2573 bp), and it would also hybridize to LiPB (1475 bp) and its' allele LPOB (1593 bp). The 28-mer hybridizes to a position 600 bp 3' of LiPA's translational stop codon. This region of intergenic LiPB-LiPA shows no homology to LPOB-LPOA (see ...
Nucleic acid analogue
Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.