An overview of the structures of protein-DNA complexes
... major groove also varies, reflecting the structural and functional requirements of each protein. The recognition helices of prokaryotic transcription factors (for example, those of the Cro and Repressor family, such as 1lli) are generally aligned with their axes parallel to base-pairing edges of the ...
... major groove also varies, reflecting the structural and functional requirements of each protein. The recognition helices of prokaryotic transcription factors (for example, those of the Cro and Repressor family, such as 1lli) are generally aligned with their axes parallel to base-pairing edges of the ...
inducers - Navin Pokala
... RNA polymerase unblocked à genes ac8ve No repressor (lacI-): cannot block RNA polymerase à cons8tu8ve expression Muta8ons (lacOc) in operator region that are unable to bind ...
... RNA polymerase unblocked à genes ac8ve No repressor (lacI-): cannot block RNA polymerase à cons8tu8ve expression Muta8ons (lacOc) in operator region that are unable to bind ...
continued
... –If breaks in chromosomes occur in introns, exons may remain intact and be spliced to other chromosomes in ways that produce new, useful proteins Biology: Life on Earth, 9e ...
... –If breaks in chromosomes occur in introns, exons may remain intact and be spliced to other chromosomes in ways that produce new, useful proteins Biology: Life on Earth, 9e ...
Structural Studies on the Dosage Compensation Complex from
... compensation is a process that equalizes gene expression from the X chromosome between males and females (Straub and Becker, 2007). Furthermore, the equal distribution of gene expression between the X chromosome and the autosomes is ensured. The process of dosage compensation has been best studied i ...
... compensation is a process that equalizes gene expression from the X chromosome between males and females (Straub and Becker, 2007). Furthermore, the equal distribution of gene expression between the X chromosome and the autosomes is ensured. The process of dosage compensation has been best studied i ...
Gene Expression in Adult Metafemales of Drosophila
... of structural genes cancels the inverse effect of the triple-X genotype. The autosomalgenes, however, show reduced expression since their copy number is unchanged. It is hypothesized that this condition contributes to the inviability of metafemales. ...
... of structural genes cancels the inverse effect of the triple-X genotype. The autosomalgenes, however, show reduced expression since their copy number is unchanged. It is hypothesized that this condition contributes to the inviability of metafemales. ...
Characteristics of tRNAs Translating the genetic code
... the codon. 64 different codons. Most codons are interpreted in same way in different organisms (universal genetic code). • 3 codons are not recognized by any tRNA: nonsense (stop) codons: UAA, UAG, UGA • 61 codons able to specify 1 of 20 aa’s, therefore most amino acids with >1 codon. • Some aa’s ha ...
... the codon. 64 different codons. Most codons are interpreted in same way in different organisms (universal genetic code). • 3 codons are not recognized by any tRNA: nonsense (stop) codons: UAA, UAG, UGA • 61 codons able to specify 1 of 20 aa’s, therefore most amino acids with >1 codon. • Some aa’s ha ...
Text S13.
... Text S13. Comparing the effect sizes on elongation speed by various factors Due to the substantial per-codon variability of ribosomal densities, we pooled different codons together to estimate the effect size of each factor concerned. These analyses were first conducted within each gene to avoid est ...
... Text S13. Comparing the effect sizes on elongation speed by various factors Due to the substantial per-codon variability of ribosomal densities, we pooled different codons together to estimate the effect size of each factor concerned. These analyses were first conducted within each gene to avoid est ...
gene-gene interaction
... When we think about factors that cause disease, we often think about specific mutations in individual genes or the environmental factors that contribute to a disease phenotype. Yet, diseasecausing mutations may not cause disease in all individuals. One possible important reason for this is that the ...
... When we think about factors that cause disease, we often think about specific mutations in individual genes or the environmental factors that contribute to a disease phenotype. Yet, diseasecausing mutations may not cause disease in all individuals. One possible important reason for this is that the ...
The effect of sodium ion concentration on
... Boltzmann cylindrical cell model for the limiting case of very low salt concentration (10, 11). The values of b for duplex B-DNA and single-stranded DNA in the open-coil form are 0.17 and 0.41 nm (9), giving values of 0.88 and 0.71 for i/^ and xj/^, respectively. Thus equation (2) predicts that A\p ...
... Boltzmann cylindrical cell model for the limiting case of very low salt concentration (10, 11). The values of b for duplex B-DNA and single-stranded DNA in the open-coil form are 0.17 and 0.41 nm (9), giving values of 0.88 and 0.71 for i/^ and xj/^, respectively. Thus equation (2) predicts that A\p ...
Translation
... Anticodoncomplementary to codon on mRNA Amino attachment (CCA) site Other recognition sites ...
... Anticodoncomplementary to codon on mRNA Amino attachment (CCA) site Other recognition sites ...
Multiplex RT-PCR kit.
... amplicons are present. Note, the reaction control band can be weak in reactions with a strong translocation specific band. The breakpoint is identified by the molecular size of the translocation specific amplicon using Interpretation Table 4. More than one translocation specific band shows presence ...
... amplicons are present. Note, the reaction control band can be weak in reactions with a strong translocation specific band. The breakpoint is identified by the molecular size of the translocation specific amplicon using Interpretation Table 4. More than one translocation specific band shows presence ...
Animals and plants manage to make copies of themselves from one
... just like themselves, so they must have some device for passing on their characteristics by inheritance. What would that device be? Until biochemists managed to get pure samples of viruses without any pieces of cells included, they couldn’t be sure what made up the viruses. Pure samples were first o ...
... just like themselves, so they must have some device for passing on their characteristics by inheritance. What would that device be? Until biochemists managed to get pure samples of viruses without any pieces of cells included, they couldn’t be sure what made up the viruses. Pure samples were first o ...
RNA Splicing
... • Another splicing factor, called SF1in mammals and BBP in yeast. connects V2AF/Mud2 to the U1 snRNP bound at the 5' splice site. Complex formation is enhanced by the cooperative reactions of the two proteins; SF 1 and U2AF (or BBP and Mud2) bind together to the RNA substrate -1 Ox more effectively ...
... • Another splicing factor, called SF1in mammals and BBP in yeast. connects V2AF/Mud2 to the U1 snRNP bound at the 5' splice site. Complex formation is enhanced by the cooperative reactions of the two proteins; SF 1 and U2AF (or BBP and Mud2) bind together to the RNA substrate -1 Ox more effectively ...
pdf
... 3. What are the contacts between the protein and the binding site in DNA? a. Methylation interference reactions: When a purine that makes contact with the protein is methylated by dimethyl sulphate (DMS), the DNA will no longer bind to the protein. Thus, DNA is gently methylated (about one hit per m ...
... 3. What are the contacts between the protein and the binding site in DNA? a. Methylation interference reactions: When a purine that makes contact with the protein is methylated by dimethyl sulphate (DMS), the DNA will no longer bind to the protein. Thus, DNA is gently methylated (about one hit per m ...
Giant DNA Lab Manual.
... Instead, you should gently pinch the backbone strands together as in the figure below. The ball and socket joints will open easily and not wear out – this is the way they were designed to open. Your TA will demonstrate. ...
... Instead, you should gently pinch the backbone strands together as in the figure below. The ball and socket joints will open easily and not wear out – this is the way they were designed to open. Your TA will demonstrate. ...
1st set of Journal Clubs this Wednesday!
... Positive control: ‘An activator causes the accelerator pedal to be pushed.’ Cis-acting sequence: Can only function if on same piece of DNA that its regulating Trans-acting factor: A gene product that can act in ‘trans’ i.e. diffuse to a location at a distance from where it was made. ...
... Positive control: ‘An activator causes the accelerator pedal to be pushed.’ Cis-acting sequence: Can only function if on same piece of DNA that its regulating Trans-acting factor: A gene product that can act in ‘trans’ i.e. diffuse to a location at a distance from where it was made. ...
genetics/dna jeopardy
... Answer E is the Messenger RNA (mRNA) and it copies the DNA, brings it to the cytoplasm, where a ribosome attaches to it and makes the protein. ...
... Answer E is the Messenger RNA (mRNA) and it copies the DNA, brings it to the cytoplasm, where a ribosome attaches to it and makes the protein. ...
DNA Shape Dominates Sequence Affinity in Nucleosome Formation
... relative to a reference state that lacks sequence dependent curvature and minor groove profile (i.e., the S model). Thus, U0i indicates the importance of the ith energy contribution to nucleosome formation. Figure 4(c) plots ΔU0 ¼ U0P − U 0D as a function of intrinsic curvature, hA0f i, for each seq ...
... relative to a reference state that lacks sequence dependent curvature and minor groove profile (i.e., the S model). Thus, U0i indicates the importance of the ith energy contribution to nucleosome formation. Figure 4(c) plots ΔU0 ¼ U0P − U 0D as a function of intrinsic curvature, hA0f i, for each seq ...
X-Chromosome Inactivation: The Case of the Calico Cat1
... The Xist/XIST gene does not code for a protein; it is transcribed into RNA, but not an RNA like any other. This mammoth transcript (17 kb in humans) contains a large number of STOP codons, such that the longest open reading frame, which could potentially code for a protein, is only 400 base pairs lo ...
... The Xist/XIST gene does not code for a protein; it is transcribed into RNA, but not an RNA like any other. This mammoth transcript (17 kb in humans) contains a large number of STOP codons, such that the longest open reading frame, which could potentially code for a protein, is only 400 base pairs lo ...
Presentation - University of Warwick
... • Can be used in the following ways: 1.To ensure superfluous parameters have minimal effect on gene expression (eg batching effects) 2.To find interesting parameters ...
... • Can be used in the following ways: 1.To ensure superfluous parameters have minimal effect on gene expression (eg batching effects) 2.To find interesting parameters ...
msc_botnay_pre_pap1_bl2
... positive charge on NH3+, while phosphorylation introduces a negative charge in the form of phosphate group. 3.6 DNA STRUCTURE THE WATSON AND CRICK’S MODEL OF DNA DOUBLE HELIX In 1953, James Watson and Francis Crick deduced the three dimensional structure of DNA and immediately inferred its mechanism ...
... positive charge on NH3+, while phosphorylation introduces a negative charge in the form of phosphate group. 3.6 DNA STRUCTURE THE WATSON AND CRICK’S MODEL OF DNA DOUBLE HELIX In 1953, James Watson and Francis Crick deduced the three dimensional structure of DNA and immediately inferred its mechanism ...
File
... of 4 bases • By 1953 Watson & Crick published the double-helical model of DNA structure and Chargaff had shown that the 4 bases were not present in equal proportions • Hershey and Chase demonstrated confirmed that DNA is the genetic material ...
... of 4 bases • By 1953 Watson & Crick published the double-helical model of DNA structure and Chargaff had shown that the 4 bases were not present in equal proportions • Hershey and Chase demonstrated confirmed that DNA is the genetic material ...
Chapter 4. The Epigenetics of Non
... whereby one daughter cell retains the stem-cell properties, and the other daughter cell is committed to a differentiated function. This behavior is controlled inter-cellularly (between cells by cell signalling), as well as intra-cellularly through epigenetic, transcriptional, translational, and post ...
... whereby one daughter cell retains the stem-cell properties, and the other daughter cell is committed to a differentiated function. This behavior is controlled inter-cellularly (between cells by cell signalling), as well as intra-cellularly through epigenetic, transcriptional, translational, and post ...
The Epigenetics of Non
... whereby one daughter cell retains the stem-cell properties, and the other daughter cell is committed to a differentiated function. This behavior is controlled inter-cellularly (between cells by cell signalling), as well as intra-cellularly through epigenetic, transcriptional, translational, and post ...
... whereby one daughter cell retains the stem-cell properties, and the other daughter cell is committed to a differentiated function. This behavior is controlled inter-cellularly (between cells by cell signalling), as well as intra-cellularly through epigenetic, transcriptional, translational, and post ...
Nucleic acid tertiary structure
The tertiary structure of a nucleic acid is its precise three-dimensional structure, as defined by the atomic coordinates. RNA and DNA molecules are capable of diverse functions ranging from molecular recognition to catalysis. Such functions require a precise three-dimensional tertiary structure. While such structures are diverse and seemingly complex, they are composed of recurring, easily recognizable tertiary structure motifs that serve as molecular building blocks. Some of the most common motifs for RNA and DNA tertiary structure are described below, but this information is based on a limited number of solved structures. Many more tertiary structural motifs will be revealed as new RNA and DNA molecules are structurally characterized.