No Slide Title - University of Michigan
... Oral Cancer Gene therapy for treatment of oral cancer and precancerous lesions (E.J. Shillitoe, Univ. Texas Dental Branch) Reasoned that therapy is likely to be more effective focused on targets expressed only in cancer cells Targeted human papillomaviruses, present in many oral neoplasms ...
... Oral Cancer Gene therapy for treatment of oral cancer and precancerous lesions (E.J. Shillitoe, Univ. Texas Dental Branch) Reasoned that therapy is likely to be more effective focused on targets expressed only in cancer cells Targeted human papillomaviruses, present in many oral neoplasms ...
Screening of Mutations and Polymorphisms in the Glucokinase
... similar in kinetic activity and are coded by the same gene with 12 exons on chromosome 7 (7p15.3-p15.1), their primary structures in the N-terminal are different due to distinct splicing of the RNA transcript. The enzymes contain 465 amino acids and exon 1 varies in the diverse tissues due to the di ...
... similar in kinetic activity and are coded by the same gene with 12 exons on chromosome 7 (7p15.3-p15.1), their primary structures in the N-terminal are different due to distinct splicing of the RNA transcript. The enzymes contain 465 amino acids and exon 1 varies in the diverse tissues due to the di ...
Performance Comparison of Two Gene Set Analysis Methods for
... permutation trials; this requires lengthy computation runs. The Z-statistics method accepts only one set of unpermuted original p-values and compares the score of a gene set against the background distribution made by all the genes; these p-values should be readily available for a typical GWAS. Simi ...
... permutation trials; this requires lengthy computation runs. The Z-statistics method accepts only one set of unpermuted original p-values and compares the score of a gene set against the background distribution made by all the genes; these p-values should be readily available for a typical GWAS. Simi ...
Using real time RT-PCR analysis to determine multiple gene
... in mammals (Berta et al., 1990; Gubbay et al., 1990). Since then, additional genes have been identified that play important roles in establishment of the genital ridge and differentiation of normal testes and ovaries (reviewed in Scherer, 2002; and primary references therein). The discovery rate for ...
... in mammals (Berta et al., 1990; Gubbay et al., 1990). Since then, additional genes have been identified that play important roles in establishment of the genital ridge and differentiation of normal testes and ovaries (reviewed in Scherer, 2002; and primary references therein). The discovery rate for ...
Male-to-male transmission of X-linked Alport syndrome in a
... due to presenting with macrohematuria from 2 years of age, proteinuria of 9 mg/h/m2 and having familial antecedents of the disease. His physical examination was normal, with average genital development for his age and no signs of hypoacusia, although due to his early age no audiometric examination h ...
... due to presenting with macrohematuria from 2 years of age, proteinuria of 9 mg/h/m2 and having familial antecedents of the disease. His physical examination was normal, with average genital development for his age and no signs of hypoacusia, although due to his early age no audiometric examination h ...
mutation and Y486D mutation in exon 5 of the UGT1A1 - Funpec-RP
... level of 1-6 mg/dL (Bosma et al., 1995; Bayram et al., 2013). CN is related to inborn errors in metabolism and are inherited as autosomal recessive traits. These traits result from unconjugated hyperbilirubinemia because of defective bilirubin uridinediphosphate (UDP)glucuronosyltransferase (UGT1A1) ...
... level of 1-6 mg/dL (Bosma et al., 1995; Bayram et al., 2013). CN is related to inborn errors in metabolism and are inherited as autosomal recessive traits. These traits result from unconjugated hyperbilirubinemia because of defective bilirubin uridinediphosphate (UDP)glucuronosyltransferase (UGT1A1) ...
Two cysteine substitutions in the MC1R generate the
... 5th position is conserved in all mammalians characterized so far, except for mouse which has a glutamine substituting the glycine. In position 280, all species are identical except for pig which has a valine substituting the phenylalanine. Based on the present data, it is not possible to conclude wh ...
... 5th position is conserved in all mammalians characterized so far, except for mouse which has a glutamine substituting the glycine. In position 280, all species are identical except for pig which has a valine substituting the phenylalanine. Based on the present data, it is not possible to conclude wh ...
The causes and molecular consequences of polyploidy in
... third generation50. On the contrary, studies in synthetic A. thaliana autopolyploids reveal little to ...
... third generation50. On the contrary, studies in synthetic A. thaliana autopolyploids reveal little to ...
A CONTRIBUTION TO AN UNDERSTANDING OF CROSSING
... the chromosomes remain unpaired in zygotene and pachytene, as DARLINGTON suggests, the chief contention of the early telosynaptists is supported in Oenothera, for the great bulk of the pachytene spireme would then be univalent. If,bn the other hand, the homologous segments are long and the greater p ...
... the chromosomes remain unpaired in zygotene and pachytene, as DARLINGTON suggests, the chief contention of the early telosynaptists is supported in Oenothera, for the great bulk of the pachytene spireme would then be univalent. If,bn the other hand, the homologous segments are long and the greater p ...
Epigenetic Mechanisms of Gene Regulation
... Five genes encoding DNMTs (including potential DNMT-like genes that may not be enzymatically active) have been identified in mammalian cells, D N M T l , 2, 3A, 3B, and 3L. ' '^^ Each gene is designated by the numbers 1, 2, 3, in the order in which they were identified. For the members of DNMT3 fami ...
... Five genes encoding DNMTs (including potential DNMT-like genes that may not be enzymatically active) have been identified in mammalian cells, D N M T l , 2, 3A, 3B, and 3L. ' '^^ Each gene is designated by the numbers 1, 2, 3, in the order in which they were identified. For the members of DNMT3 fami ...
Saccharomyces Genome Database (SGD) provides secondary gene annotation using the Gene Ontology (GO).
... approach of requiring exact matches, many of these were associations to the ‘molecular_function unknown’ and ‘biological_process unknown’ terms. However, 639 gene products received biological process and molecular function terms other than ‘unknown’. The second set of IEA associations were produced ...
... approach of requiring exact matches, many of these were associations to the ‘molecular_function unknown’ and ‘biological_process unknown’ terms. However, 639 gene products received biological process and molecular function terms other than ‘unknown’. The second set of IEA associations were produced ...
International Journal of Antimicrobial Agents Prevalence of
... there was a conspicuous absence of NAL-resistant S. Enteritidis, although the reason for this finding remains unclear. Nevertheless, the overall frequency of resistance to nalidixic acid amounted to 2.2% and thus appears to be relatively low in Serbia compared with other countries in East and West Eu ...
... there was a conspicuous absence of NAL-resistant S. Enteritidis, although the reason for this finding remains unclear. Nevertheless, the overall frequency of resistance to nalidixic acid amounted to 2.2% and thus appears to be relatively low in Serbia compared with other countries in East and West Eu ...
Candidate gene screening using long-read sequencing
... - Both isotypes can have 1 - 3 functional copies - A human endogenous retroviral (HERV) insertion in intron 9 changes the length of the gene ...
... - Both isotypes can have 1 - 3 functional copies - A human endogenous retroviral (HERV) insertion in intron 9 changes the length of the gene ...
Point Mutation Analysis of PMP22 in Patients Referred for Hereditary
... c.392C>G p.(Ser131Cys) and c.395A>G p.(Tyr132Cys)), while the c.320-1_320delGGinsTA (destruction of splice site) and c.434delT (protein reading frame shift) mutations are clearly deleterious. The remaining base change, c.79-13T>A, is potentially deleterious based on the in silico data, but RNA analy ...
... c.392C>G p.(Ser131Cys) and c.395A>G p.(Tyr132Cys)), while the c.320-1_320delGGinsTA (destruction of splice site) and c.434delT (protein reading frame shift) mutations are clearly deleterious. The remaining base change, c.79-13T>A, is potentially deleterious based on the in silico data, but RNA analy ...
Linkage
... modern times, we can use the locations to clone the genes so we can better understand what they do and why they cause genetic diseases when mutated. The basis of linkage mapping is that since crossing over occurs at random locations, the closer two genes are to each other, the less likely it is that ...
... modern times, we can use the locations to clone the genes so we can better understand what they do and why they cause genetic diseases when mutated. The basis of linkage mapping is that since crossing over occurs at random locations, the closer two genes are to each other, the less likely it is that ...
HIGH FREQUENCY GENE TARGETING USING INSERTIONAL
... an insertional vector containing 3.5 kb of isogenic DNA (10). The frequency of homologous recombination was 1 in 46 (2.2%). This frequency is significantly higher than those reported by two other groups using isogenic DNA sequence replacement constructs (11,12). All three groups were targeting the ...
... an insertional vector containing 3.5 kb of isogenic DNA (10). The frequency of homologous recombination was 1 in 46 (2.2%). This frequency is significantly higher than those reported by two other groups using isogenic DNA sequence replacement constructs (11,12). All three groups were targeting the ...
File
... b) The RNA fragments enhance protein synthesis by the mRNA. c) The RNA fragments bind the ribosome to enhance use of the mRNA and protein synthesis. d) The target mRNA is blocked from being used in translation. e) The RNA fragments act on the ribosome to shut down translation of all mRNAs. © 2014 Pe ...
... b) The RNA fragments enhance protein synthesis by the mRNA. c) The RNA fragments bind the ribosome to enhance use of the mRNA and protein synthesis. d) The target mRNA is blocked from being used in translation. e) The RNA fragments act on the ribosome to shut down translation of all mRNAs. © 2014 Pe ...
Novel mutations in the folliculin gene associated with spontaneous pneumothorax ´tya
... human organs and tissues including skin, lung and kidney [20]. In the lung, FLCN is transcribed in type-1 pneumocytes and stroma cells, including fibroblasts and macrophages. An imbalance may either induce an inflammatory response or alter matrix degradation and remodelling. A variety of mutations i ...
... human organs and tissues including skin, lung and kidney [20]. In the lung, FLCN is transcribed in type-1 pneumocytes and stroma cells, including fibroblasts and macrophages. An imbalance may either induce an inflammatory response or alter matrix degradation and remodelling. A variety of mutations i ...
14 PCA and K-Means Decipher Genome
... The information that is needed for a living cell functioning is encoded in a long molecule of DNA. It can be presented as a text with an alphabet that has only four letters A, C, G and T. The diversity of living organisms and their complex properties is hidden in their genomic sequences. One of the ...
... The information that is needed for a living cell functioning is encoded in a long molecule of DNA. It can be presented as a text with an alphabet that has only four letters A, C, G and T. The diversity of living organisms and their complex properties is hidden in their genomic sequences. One of the ...
as a PDF
... deferens. However, there is a considerable diversity among different populations and the role of other potential causes is not ruled out yet. Autosomal and X-linked genetic aberrations in men with idiopathic hypogonadotropic hypogonadism are now well known. As hormone replacement therapy can provide ...
... deferens. However, there is a considerable diversity among different populations and the role of other potential causes is not ruled out yet. Autosomal and X-linked genetic aberrations in men with idiopathic hypogonadotropic hypogonadism are now well known. As hormone replacement therapy can provide ...
towards synthetic plant genomes, transcriptomes and epigenomes
... It is possible to target individual sequence motives within genomes by using synthetic DNA-binding domains. This one-dimensional approach has been used successfully in plants to induce mutations or for the transcriptional regulation of single genes. When the CRISPR (clustered regularly interspaced s ...
... It is possible to target individual sequence motives within genomes by using synthetic DNA-binding domains. This one-dimensional approach has been used successfully in plants to induce mutations or for the transcriptional regulation of single genes. When the CRISPR (clustered regularly interspaced s ...
A dioxin sensitive gene, mammalian WAPL, is implicated in
... examined mWAPL mRNA levels in AhR+/+ and AhR/ MEFs treated with 0, 0.01, 0.1, and 1 lM TCDD for 2 h by Northern blot analysis. Although mWAPL signals were on the whole extremely weak and barely visualized by strong enhancement, we found that mWAPL mRNA levels in AhR/ MEFs showed the highest at 1 ...
... examined mWAPL mRNA levels in AhR+/+ and AhR/ MEFs treated with 0, 0.01, 0.1, and 1 lM TCDD for 2 h by Northern blot analysis. Although mWAPL signals were on the whole extremely weak and barely visualized by strong enhancement, we found that mWAPL mRNA levels in AhR/ MEFs showed the highest at 1 ...
Deep Insight Section Genomic Imprinting: Parental differentiation of the genome
... Figure 11: 15mat imprinted domain: Physical examination of the imprinted domain on maternally inherited chromosome 15 reveals DNA cytosine methylation, histone H3 tail methylation at lysine 9, recruitment of histone deacetylating enzymes, and deacetylated histones. These features are typical of clos ...
... Figure 11: 15mat imprinted domain: Physical examination of the imprinted domain on maternally inherited chromosome 15 reveals DNA cytosine methylation, histone H3 tail methylation at lysine 9, recruitment of histone deacetylating enzymes, and deacetylated histones. These features are typical of clos ...
publication
... genes. All the active genes have at least three exons. Two introns are found in the erythroid globin genes and MB; although they differ dramatically in size, they are in homologous locations (Figure 3). Furthermore, even more distantly related globin genes, such as CYGB and NGB in vertebrates and pl ...
... genes. All the active genes have at least three exons. Two introns are found in the erythroid globin genes and MB; although they differ dramatically in size, they are in homologous locations (Figure 3). Furthermore, even more distantly related globin genes, such as CYGB and NGB in vertebrates and pl ...
Oncogenomics
Oncogenomics is a relatively new sub-field of genomics that applies high throughput technologies to characterize genes associated with cancer. Oncogenomics is synonymous with ""cancer genomics"". Cancer is a genetic disease caused by accumulation of mutations to DNA leading to unrestrained cell proliferation and neoplasm formation. The goal of oncogenomics is to identify new oncogenes or tumor suppressor genes that may provide new insights into cancer diagnosis, predicting clinical outcome of cancers, and new targets for cancer therapies. The success of targeted cancer therapies such as Gleevec, Herceptin, and Avastin raised the hope for oncogenomics to elucidate new targets for cancer treatment.Besides understanding the underlying genetic mechanisms that initiates or drives cancer progression, one of the main goals of oncogenomics is to allow for the development of personalized cancer treatment. Cancer develops due to an accumulation of mutations in DNA. These mutations accumulate randomly, and thus, different DNA mutations and mutation combinations exist between different individuals with the same type of cancer. Thus, identifying and targeting specific mutations which have occurred in an individual patient may lead to increased efficacy of cancer therapy.The completion of the Human Genome Project has greatly facilitated the field of oncogenomics and has increased the abilities of researchers to find cancer causing genes. In addition, the sequencing technologies now available for sequence generation and data analysis have been applied to the study of oncogenomics. With the amount of research conducted on cancer genomes and the accumulation of databases documenting the mutational changes, it has been predicted that the most important cancer-causing mutations, rearrangements, and altered expression levels will be cataloged and well characterized within the next decade.Cancer research may look either on the genomic level at DNA mutations, the epigenetic level at methylation or histone modification changes, the transcription level at altered levels of gene expression, or the protein level at altered levels of protein abundance and function in cancer cells. Oncogenomics focuses on the genomic, epigenomic, and transcript level alterations in cancer.