Nomenclature of Transposable Elements in Prokaryotes
... name such as IS2 is a generic term referring to all insertions that appear indistinguishable by hybridization, heteroduplexing, or restriction analysis, but which could have individual differences in base sequence and which might have undergone mutations or rearrangements in the laboratory (seeIV. E ...
... name such as IS2 is a generic term referring to all insertions that appear indistinguishable by hybridization, heteroduplexing, or restriction analysis, but which could have individual differences in base sequence and which might have undergone mutations or rearrangements in the laboratory (seeIV. E ...
Document
... The duplication or repositioning of exons has contributed to genome evolution Errors in meiosis can result in an exon being duplicated on one chromosome and deleted from the homologous chromosome In exon shuffling, errors in meiotic recombination lead to some mixing and matching of exons, eith ...
... The duplication or repositioning of exons has contributed to genome evolution Errors in meiosis can result in an exon being duplicated on one chromosome and deleted from the homologous chromosome In exon shuffling, errors in meiotic recombination lead to some mixing and matching of exons, eith ...
Feb 27 lecture presentation
... • Distinguish between positive and negative transcriptional regulation • Make predictions based on hypotheses • Infer protein function from mutant phenotype Pages 519-525 of textbook ...
... • Distinguish between positive and negative transcriptional regulation • Make predictions based on hypotheses • Infer protein function from mutant phenotype Pages 519-525 of textbook ...
lectureFeb27
... • Distinguish between positive and negative transcriptional regulation • Make predictions based on hypotheses • Infer protein function from mutant phenotype Pages 519-525 of textbook ...
... • Distinguish between positive and negative transcriptional regulation • Make predictions based on hypotheses • Infer protein function from mutant phenotype Pages 519-525 of textbook ...
brief review - AJP
... the cell. N-linked sugar residues are present on the large extracellular loop between the 7th and 8th membrane-spanning segment: two for the Na-Cl cotransporter and up to three for the Na-K-2Cl cotransporter. The specific ion and diuretic binding regions on these cotransporters have not been identif ...
... the cell. N-linked sugar residues are present on the large extracellular loop between the 7th and 8th membrane-spanning segment: two for the Na-Cl cotransporter and up to three for the Na-K-2Cl cotransporter. The specific ion and diuretic binding regions on these cotransporters have not been identif ...
Initial Stages in Creating a lacI Knockout in Escherichia coli C29
... required for assays. To create this mutant strain, a newly developed system for gene deletion could be employed. The λ Red recombination technology has been used in E. coli and salmonella for easy PCR mediated deletion mutations (6, 8). The system has three major proteins involved in the process (7) ...
... required for assays. To create this mutant strain, a newly developed system for gene deletion could be employed. The λ Red recombination technology has been used in E. coli and salmonella for easy PCR mediated deletion mutations (6, 8). The system has three major proteins involved in the process (7) ...
Cystic fibrosis - patient information
... of life for people with CF, it continues to be a serious condition. Carrier parents may wish to consider their options regarding pregnancy. Some people opt to take a chance and hope the baby does not have CF. With a one in four chance, the odds are three to one in their favour. Some choose not to ha ...
... of life for people with CF, it continues to be a serious condition. Carrier parents may wish to consider their options regarding pregnancy. Some people opt to take a chance and hope the baby does not have CF. With a one in four chance, the odds are three to one in their favour. Some choose not to ha ...
Regulation of bolting and identification of the α
... A. thaliana genes in B. rapa could be obtained from BRAD, a data base containing B. rapa genes. Each TUA gene loci in A. thaliana was used to search all the TUA gene sequences of B. rapa present in BRAD. Each predicted B. rapa TUA gene sequence was confirmed using FGENESH (http://www.softberry.com/b ...
... A. thaliana genes in B. rapa could be obtained from BRAD, a data base containing B. rapa genes. Each TUA gene loci in A. thaliana was used to search all the TUA gene sequences of B. rapa present in BRAD. Each predicted B. rapa TUA gene sequence was confirmed using FGENESH (http://www.softberry.com/b ...
The Concept of the Gene in Development and Evolution
... each regulates expression in a tissue-specific manner, leading to production of polypeptides that vary markedly in size. The many products are considered to arise from a single gene, not a set of different genes that share many parts. In addition to sliding edges on the transcript, multiple polypept ...
... each regulates expression in a tissue-specific manner, leading to production of polypeptides that vary markedly in size. The many products are considered to arise from a single gene, not a set of different genes that share many parts. In addition to sliding edges on the transcript, multiple polypept ...
Deep Insight Section RET point mutations in Thyroid Carcinoma
... The number and type of RET mutations have been grown over the last 10 years, especially after the introduction of RET genetic screening in the work up of all patients with MTC, both hereditary and apparently sporadic. As consequence of this more careful research, RET mutations have been found to be ...
... The number and type of RET mutations have been grown over the last 10 years, especially after the introduction of RET genetic screening in the work up of all patients with MTC, both hereditary and apparently sporadic. As consequence of this more careful research, RET mutations have been found to be ...
Gene Section EXT2 (exostoses (multiple) 2) Atlas of Genetics and Cytogenetics
... Function A tumour suppressor function is suggested; exostosin-2 (EXT2) is an endoplasmic reticulum localized type II transmembrane glycoprotein which together with exostosin-1 (EXT1) forms a Golgi-localized heterooligomeric complex that catalyzes heparan sulphate (HS) polymerization. It is thus hypo ...
... Function A tumour suppressor function is suggested; exostosin-2 (EXT2) is an endoplasmic reticulum localized type II transmembrane glycoprotein which together with exostosin-1 (EXT1) forms a Golgi-localized heterooligomeric complex that catalyzes heparan sulphate (HS) polymerization. It is thus hypo ...
Genetic transfer and genome evolution in MRSA
... of MRSA isolates (3). When the five genomes are compared, the total number of SNPs (31\2645) in all ISR types (Table 1a) is similar to the total number of SNPs (43\3818) found in the seven housekeeping genes (Table 1b). In contrast to the ISR, only one copy of each housekeeping gene is found on each ...
... of MRSA isolates (3). When the five genomes are compared, the total number of SNPs (31\2645) in all ISR types (Table 1a) is similar to the total number of SNPs (43\3818) found in the seven housekeeping genes (Table 1b). In contrast to the ISR, only one copy of each housekeeping gene is found on each ...
Genetic Portrait of a Yeast
... Mutations can be isolated in diploids, then analyzed in haploids Conditional mutants can be isolated Recombination frequencies are high, facilitating molecular manipulations Genome size is small and number of genes low, so analysis and manipulations of the whole genome are possible Copyright © The M ...
... Mutations can be isolated in diploids, then analyzed in haploids Conditional mutants can be isolated Recombination frequencies are high, facilitating molecular manipulations Genome size is small and number of genes low, so analysis and manipulations of the whole genome are possible Copyright © The M ...
achondroplasia
... • In more than 80 percent of cases, however, achondroplasia is not inherited but results from a new mutation (change) that occurred in the egg or sperm cell that formed the embryo. The parents of children with achondroplasia resulting from new mutations are usually normal-sized. Typically, these par ...
... • In more than 80 percent of cases, however, achondroplasia is not inherited but results from a new mutation (change) that occurred in the egg or sperm cell that formed the embryo. The parents of children with achondroplasia resulting from new mutations are usually normal-sized. Typically, these par ...
Initial manifestation of DCM caused by a mutation in lamin A/C
... Identification of mutation carriers may lead to early prevention of SCD. ...
... Identification of mutation carriers may lead to early prevention of SCD. ...
Polymorphisms of the bovine growth differentiation factor 9 gene
... mothers of dizygotic twins, and those variants are significantly related to increased ovulation rate (Palmer et al., 2006), which suggests that some variants may also be linked to a polyovulatory phenotype (Montgomery et al., 2004). Furthermore, Wang et al. (2010) have found that the GDF9 G546A muta ...
... mothers of dizygotic twins, and those variants are significantly related to increased ovulation rate (Palmer et al., 2006), which suggests that some variants may also be linked to a polyovulatory phenotype (Montgomery et al., 2004). Furthermore, Wang et al. (2010) have found that the GDF9 G546A muta ...
Crumbs and Xpd in mitosis
... segregation in all multicellular organisms. Misregulation of mitosis can cause genetic instability and cancer. Thus, identification of the genes involved in the regulation of mitosis is important for understanding the mechanism of chromosome segregation and genome instability. Unbiased genetic scree ...
... segregation in all multicellular organisms. Misregulation of mitosis can cause genetic instability and cancer. Thus, identification of the genes involved in the regulation of mitosis is important for understanding the mechanism of chromosome segregation and genome instability. Unbiased genetic scree ...
Reconstructing evolution: Gene transfer from plastids to the nucleus
... the mitochondria, which stem from an a-proteobacterium, and the plastids (chloroplasts), which are derived from a cyanobacterium (Fig. 1). Early genetic and biochemical studies revealed that the genomes of plastids have been greatly diminished compared with any possible free-living ancestor. The pla ...
... the mitochondria, which stem from an a-proteobacterium, and the plastids (chloroplasts), which are derived from a cyanobacterium (Fig. 1). Early genetic and biochemical studies revealed that the genomes of plastids have been greatly diminished compared with any possible free-living ancestor. The pla ...
DNA Methylation Maintains Allele-specific KIR Gene Expression in
... were provided by Z. Brahmi, University of Indiana Medical Center, Indianapolis, IN. NK-92 subclones and YT-Indy cells were treated with 5-aza-2-deoxycytidine (Aza; Sigma-Aldrich) and analyzed by flow cytometry or cloned as previously described (20, 21). mAbs were obtained commercially (Immunotech o ...
... were provided by Z. Brahmi, University of Indiana Medical Center, Indianapolis, IN. NK-92 subclones and YT-Indy cells were treated with 5-aza-2-deoxycytidine (Aza; Sigma-Aldrich) and analyzed by flow cytometry or cloned as previously described (20, 21). mAbs were obtained commercially (Immunotech o ...
Primary sex determination in the nematode C. elegans
... in XXY male mammals. In Drosophila, dosage compensation is achieved by increasing transcription from the single X chromosome of XY flies, to match the total output of the two X chromosomes of XX flies. Failure to compensate correctly is lethal. Both sex and compensation are controlled by a single 'm ...
... in XXY male mammals. In Drosophila, dosage compensation is achieved by increasing transcription from the single X chromosome of XY flies, to match the total output of the two X chromosomes of XX flies. Failure to compensate correctly is lethal. Both sex and compensation are controlled by a single 'm ...
Characterization of Two ENU-Induced Mutations Affecting Mouse
... oxygen tension, exposure to teratogens, and prenatal vitamin deficiencies, can all contribute to congenital defects (Hensinger 2009), the genetic contribution to the pathogenesis of vertebral malformation syndromes is less understood. This is largely attributable to the fact that, in humans, vertebra ...
... oxygen tension, exposure to teratogens, and prenatal vitamin deficiencies, can all contribute to congenital defects (Hensinger 2009), the genetic contribution to the pathogenesis of vertebral malformation syndromes is less understood. This is largely attributable to the fact that, in humans, vertebra ...
Escherichia coli synthetic genetic array
... plates and quantified the data using an automated image processing system originally devised for yeast6. Although synthetic lethality is straightforward to score visually (that is, no viable colony is detected), our automated quantitative scoring system greatly improved the informational content per ...
... plates and quantified the data using an automated image processing system originally devised for yeast6. Although synthetic lethality is straightforward to score visually (that is, no viable colony is detected), our automated quantitative scoring system greatly improved the informational content per ...
Oncogenomics
Oncogenomics is a relatively new sub-field of genomics that applies high throughput technologies to characterize genes associated with cancer. Oncogenomics is synonymous with ""cancer genomics"". Cancer is a genetic disease caused by accumulation of mutations to DNA leading to unrestrained cell proliferation and neoplasm formation. The goal of oncogenomics is to identify new oncogenes or tumor suppressor genes that may provide new insights into cancer diagnosis, predicting clinical outcome of cancers, and new targets for cancer therapies. The success of targeted cancer therapies such as Gleevec, Herceptin, and Avastin raised the hope for oncogenomics to elucidate new targets for cancer treatment.Besides understanding the underlying genetic mechanisms that initiates or drives cancer progression, one of the main goals of oncogenomics is to allow for the development of personalized cancer treatment. Cancer develops due to an accumulation of mutations in DNA. These mutations accumulate randomly, and thus, different DNA mutations and mutation combinations exist between different individuals with the same type of cancer. Thus, identifying and targeting specific mutations which have occurred in an individual patient may lead to increased efficacy of cancer therapy.The completion of the Human Genome Project has greatly facilitated the field of oncogenomics and has increased the abilities of researchers to find cancer causing genes. In addition, the sequencing technologies now available for sequence generation and data analysis have been applied to the study of oncogenomics. With the amount of research conducted on cancer genomes and the accumulation of databases documenting the mutational changes, it has been predicted that the most important cancer-causing mutations, rearrangements, and altered expression levels will be cataloged and well characterized within the next decade.Cancer research may look either on the genomic level at DNA mutations, the epigenetic level at methylation or histone modification changes, the transcription level at altered levels of gene expression, or the protein level at altered levels of protein abundance and function in cancer cells. Oncogenomics focuses on the genomic, epigenomic, and transcript level alterations in cancer.