The polymorphism in MUC1 gene in Nelore cattle
... MUC1 is a transmembrane glycoprotein expressed on the apical surfaces of the uterine epithelial tissue with predicted functions in protection and cell–cell adhesion. These properties are closely related with the repetitive region [variable number of tandem repeats (VNTR)] of the extracellullar domai ...
... MUC1 is a transmembrane glycoprotein expressed on the apical surfaces of the uterine epithelial tissue with predicted functions in protection and cell–cell adhesion. These properties are closely related with the repetitive region [variable number of tandem repeats (VNTR)] of the extracellullar domai ...
PINK1 positively regulates HDAC3 to suppress p53
... deacetylase activity. We demonstrate that PINK1 prevents H2O2-induced C-terminal cleavage of HDAC3 by inhibiting caspase-7 activity, which was reversed by protein phosphatase 4c (PP4c). PINK1-mediated phosphorylation of HDAC3 enhances its direct association with p53 and causes subsequent hypoacetyla ...
... deacetylase activity. We demonstrate that PINK1 prevents H2O2-induced C-terminal cleavage of HDAC3 by inhibiting caspase-7 activity, which was reversed by protein phosphatase 4c (PP4c). PINK1-mediated phosphorylation of HDAC3 enhances its direct association with p53 and causes subsequent hypoacetyla ...
ZOO-302CR:(1.4)CELL DIVISION AND CELL CYCLE
... the activation of genes essential for DNA synthesis and cell cycle progression. E2F complexed with hypophosphorylated Rb cannot activate transcription. Hyperphosphorylation of Rb causes dissociation from E2F. Cell cycle checkpoints are shown as shaded bars. , inhibition step; _, activation step; Cdk ...
... the activation of genes essential for DNA synthesis and cell cycle progression. E2F complexed with hypophosphorylated Rb cannot activate transcription. Hyperphosphorylation of Rb causes dissociation from E2F. Cell cycle checkpoints are shown as shaded bars. , inhibition step; _, activation step; Cdk ...
NAME: AKALABU, MAUREEN CHIDINMA COURSE: BCH 301 MAT
... discarded, while the two flanking RNA pieces (called exons) are ligated together. This reaction is called splicing. Besides ribozyme-mediated splicing, which involves RNA alone; there are some splicing reactions that involve RNA-protein complexes. These complexes are called small nucleus ribonucleop ...
... discarded, while the two flanking RNA pieces (called exons) are ligated together. This reaction is called splicing. Besides ribozyme-mediated splicing, which involves RNA alone; there are some splicing reactions that involve RNA-protein complexes. These complexes are called small nucleus ribonucleop ...
Detailed proteome analysis of growing cells of the planctomycete
... functionally categorized using Clusters of Orthologous Groups (COGs), and their potential cellular locations were predicted by bioinformatic tools. A unique protein family that contains several YTV domains and is rich in cysteine and proline was found to be a component of the R. baltica proteinaceou ...
... functionally categorized using Clusters of Orthologous Groups (COGs), and their potential cellular locations were predicted by bioinformatic tools. A unique protein family that contains several YTV domains and is rich in cysteine and proline was found to be a component of the R. baltica proteinaceou ...
PhD Thesis - Cox Group
... Introduction The term ‘natural product’ means molecules of life1 but in general it refers to ...
... Introduction The term ‘natural product’ means molecules of life1 but in general it refers to ...
Chlamydia trachomatis RNA polymerase major sigma subunit
... noted, both clinically and in tissue culture, although the mechanism by which this occurs is unknown (4). Several gene products that are temporally regulated and associated with RB:EB differentiation have been characterized. We have previously shown that differential transcription of the chlamydial ...
... noted, both clinically and in tissue culture, although the mechanism by which this occurs is unknown (4). Several gene products that are temporally regulated and associated with RB:EB differentiation have been characterized. We have previously shown that differential transcription of the chlamydial ...
Triphosphatase Related to the Protein Tyrosine Phosphatases
... CEG1 and ABD1 are essential for viability. Sequence analysis, biochemical characterization, and genetic experiments have shown that the yeast guanylyltransferases are mechanistically related to DNA and RNA ligases (Shuman and Schwer, 1995, for review). The yeast guanylyltransferase proteins do not h ...
... CEG1 and ABD1 are essential for viability. Sequence analysis, biochemical characterization, and genetic experiments have shown that the yeast guanylyltransferases are mechanistically related to DNA and RNA ligases (Shuman and Schwer, 1995, for review). The yeast guanylyltransferase proteins do not h ...
Engineering primary metabolic pathways of industrial
... genes that confer a prominent phenotype when the responsible mutation(s) are anywhere on the genome, but not at the place of the coding gene itself. For example, over-expression of certain sigma factors enhanced protein production in stationary phase (Weikert et al., 2000). Screening of libraries to ...
... genes that confer a prominent phenotype when the responsible mutation(s) are anywhere on the genome, but not at the place of the coding gene itself. For example, over-expression of certain sigma factors enhanced protein production in stationary phase (Weikert et al., 2000). Screening of libraries to ...
Sample Chapters - Pearson Canada
... As noted above, a principal task of the biochemist is to understand how a cell regulates its myriad reaction sequences and, in so doing, controls its internal environment. In Chapter 11 we discussed the properties of individual enzymes and control mechanisms that affect their activity. Here we consi ...
... As noted above, a principal task of the biochemist is to understand how a cell regulates its myriad reaction sequences and, in so doing, controls its internal environment. In Chapter 11 we discussed the properties of individual enzymes and control mechanisms that affect their activity. Here we consi ...
10 Fungal Genetics Newsletter mcm
... Microbiol. 133: 255-259), the technique has continuously improved. Genetic transformation has been used as a tool to clone a number of genes by complementation (Vollmer and Yanofsky 1986 Proc. Natl. Acad. Sci. U.S.A. 83: 4869-4873), as well as to reveal new phenomena such as RIP (Selker et al.1987 C ...
... Microbiol. 133: 255-259), the technique has continuously improved. Genetic transformation has been used as a tool to clone a number of genes by complementation (Vollmer and Yanofsky 1986 Proc. Natl. Acad. Sci. U.S.A. 83: 4869-4873), as well as to reveal new phenomena such as RIP (Selker et al.1987 C ...
Cell-Free Protein Expression Systems
... In RRL translation reactions, mRNA is used as template for translation. In general, optimal results will be achieved after an incubation time of 1.5 hours at 30°C. However, many template-related factors affect translation efficiency of specific mRNAs in the RRL system and should be considered when d ...
... In RRL translation reactions, mRNA is used as template for translation. In general, optimal results will be achieved after an incubation time of 1.5 hours at 30°C. However, many template-related factors affect translation efficiency of specific mRNAs in the RRL system and should be considered when d ...
TCA (Krebs) Cycle
... ©Copyright 1999-2004 by Gene C. Lavers No part of this presentation may be reproduced by any mechanical, photographic, or electronic process, or in the form of a phonographic recording, nor may it be stored in a retrieval system, transmitted, or otherwise copied for public or private use, without wr ...
... ©Copyright 1999-2004 by Gene C. Lavers No part of this presentation may be reproduced by any mechanical, photographic, or electronic process, or in the form of a phonographic recording, nor may it be stored in a retrieval system, transmitted, or otherwise copied for public or private use, without wr ...
general biology - Palomar College
... viruses, killing itself in the process, and liberating more viruses. Because viruses are not classic organisms, they do not respond to treatments such as antibiotics that are effective against organisms, like bacteria. ...
... viruses, killing itself in the process, and liberating more viruses. Because viruses are not classic organisms, they do not respond to treatments such as antibiotics that are effective against organisms, like bacteria. ...
Involvement of Endogenous Retroviruses in Prion Diseases
... ERV expression has been reported and has emerged as a possible etiological factor in complex diseases [36–38]. In the mouse, a number of ERVs are still active, and those with infectious counterparts, such as MuLV and MMTV, have been notably studied for decades. Several lines of evidence show that, i ...
... ERV expression has been reported and has emerged as a possible etiological factor in complex diseases [36–38]. In the mouse, a number of ERVs are still active, and those with infectious counterparts, such as MuLV and MMTV, have been notably studied for decades. Several lines of evidence show that, i ...
Supporting information S1.
... Supporting information S1. Detailed explanation of plasmids and strains construction The suicide vector pKNG101 was used to introduce the CAT* reporter gene within the Escherichia coli chromosome (Table S2). This plasmid contains a defective pir minus origin of replication (oriR6K), the strAB genes ...
... Supporting information S1. Detailed explanation of plasmids and strains construction The suicide vector pKNG101 was used to introduce the CAT* reporter gene within the Escherichia coli chromosome (Table S2). This plasmid contains a defective pir minus origin of replication (oriR6K), the strAB genes ...
Evolutionary Forces in Shaping the Codon and Amino Acid Usages
... In contrast, it has been reported that gene expression does not have any effect in influencing the codon usages in endosymbiotic bacteria Wigglesworthia glossinidia brevipalpis (20). A strong effect on codon usages has been reported due to the strand specific mutational biases in different organisms ...
... In contrast, it has been reported that gene expression does not have any effect in influencing the codon usages in endosymbiotic bacteria Wigglesworthia glossinidia brevipalpis (20). A strong effect on codon usages has been reported due to the strand specific mutational biases in different organisms ...
video slide - Buena Park High School
... • Beadle and Tatum developed the “one gene– one enzyme hypothesis” (later proven false) – Which states that the function of a gene is to dictate the production of a specific enzyme ...
... • Beadle and Tatum developed the “one gene– one enzyme hypothesis” (later proven false) – Which states that the function of a gene is to dictate the production of a specific enzyme ...
Introduction to Carbohydrates
... - Hydrogen peroxide is one of a family of reactive oxygen species that are formed from the partial reduction of molecular oxygen. - These cpds are formed continuously as by-products of aerobic metabolism, through reactions with drugs & environmental toxins, or when level of antioxidants is diminishe ...
... - Hydrogen peroxide is one of a family of reactive oxygen species that are formed from the partial reduction of molecular oxygen. - These cpds are formed continuously as by-products of aerobic metabolism, through reactions with drugs & environmental toxins, or when level of antioxidants is diminishe ...
Insulin-like growth factor binding protein-2 (IGFBP
... manufacturers’ instructions. According to the IGFBP-2s cDNA sequences of P. olivaceus transcriptome, two pairs of primers (IGFBP-2a-f, IGFBP-2a-r and IGFBP2b-f, IGFBP-2b-r) were designed in non-conservative region for cloning and verifying the partial fragment of P. olivaceus IGFBP-2. In PCR amplifi ...
... manufacturers’ instructions. According to the IGFBP-2s cDNA sequences of P. olivaceus transcriptome, two pairs of primers (IGFBP-2a-f, IGFBP-2a-r and IGFBP2b-f, IGFBP-2b-r) were designed in non-conservative region for cloning and verifying the partial fragment of P. olivaceus IGFBP-2. In PCR amplifi ...
attachment 2 - Food Standards Australia New Zealand
... Soybean is grown as a commercial food and feed crop in over 35 countries worldwide (OECD, 2001) and has a long history of safe use for both humans and livestock. The major producers of soybeans, accounting for 90% of world production, are the U.S., Argentina, Brazil and China. Australia, while a net ...
... Soybean is grown as a commercial food and feed crop in over 35 countries worldwide (OECD, 2001) and has a long history of safe use for both humans and livestock. The major producers of soybeans, accounting for 90% of world production, are the U.S., Argentina, Brazil and China. Australia, while a net ...
Thermostable glycerol kinase from a
... to the termination codon TGA are shown in italics. The resultant 1.5-kb DNA fragment was digested with NcoI and BamHI and cloned into the NcoI–BamHI site of plasmid pET8c to create plasmid pET-pkgk. An overproducing strain for Pk-GK was constructed by transforming E.coli BL21(DE3) with this plasmid. ...
... to the termination codon TGA are shown in italics. The resultant 1.5-kb DNA fragment was digested with NcoI and BamHI and cloned into the NcoI–BamHI site of plasmid pET8c to create plasmid pET-pkgk. An overproducing strain for Pk-GK was constructed by transforming E.coli BL21(DE3) with this plasmid. ...
Counting Small RNA in Pathogenic Bacteria
... unbound probes within bacterial cells. The technique is an extension of a similar procedure applied by Nolan et al. to detect mRNA in solution.11 Figure 1b illustrates how this method reduces background and false positive detection of sRNA. The method relies upon the likelihood that unbound fluoresce ...
... unbound probes within bacterial cells. The technique is an extension of a similar procedure applied by Nolan et al. to detect mRNA in solution.11 Figure 1b illustrates how this method reduces background and false positive detection of sRNA. The method relies upon the likelihood that unbound fluoresce ...
Chromosomal rearrangements and protein globularity changes in
... genes led to amino acid changes but only two altered genes have known functions: PE_PGRS19, a putative outer membrane protein (Song et al., 2008) and embR which is involved in transcription, the biosynthesis of mycobacterial cell wall arabinan and resistance to ethambutol (Table S6). The amino acid ...
... genes led to amino acid changes but only two altered genes have known functions: PE_PGRS19, a putative outer membrane protein (Song et al., 2008) and embR which is involved in transcription, the biosynthesis of mycobacterial cell wall arabinan and resistance to ethambutol (Table S6). The amino acid ...
Gene regulatory network
A gene regulatory network or genetic regulatory network (GRN) is a collection of regulators thatinteract with each other and with other substances in the cell to govern the gene expression levels of mRNA and proteins.The regulator can be DNA, RNA, protein and their complex. The interaction can be direct or indirect (through their transcribed RNA or translated protein).In general, each mRNA molecule goes on to make a specific protein (or set of proteins). In some cases this protein will be structural, and will accumulate at the cell membrane or within the cell to give it particular structural properties. In other cases the protein will be an enzyme, i.e., a micro-machine that catalyses a certain reaction, such as the breakdown of a food source or toxin. Some proteins though serve only to activate other genes, and these are the transcription factors that are the main players in regulatory networks or cascades. By binding to the promoter region at the start of other genes they turn them on, initiating the production of another protein, and so on. Some transcription factors are inhibitory.In single-celled organisms, regulatory networks respond to the external environment, optimising the cell at a given time for survival in this environment. Thus a yeast cell, finding itself in a sugar solution, will turn on genes to make enzymes that process the sugar to alcohol. This process, which we associate with wine-making, is how the yeast cell makes its living, gaining energy to multiply, which under normal circumstances would enhance its survival prospects.In multicellular animals the same principle has been put in the service of gene cascades that control body-shape. Each time a cell divides, two cells result which, although they contain the same genome in full, can differ in which genes are turned on and making proteins. Sometimes a 'self-sustaining feedback loop' ensures that a cell maintains its identity and passes it on. Less understood is the mechanism of epigenetics by which chromatin modification may provide cellular memory by blocking or allowing transcription. A major feature of multicellular animals is the use of morphogen gradients, which in effect provide a positioning system that tells a cell where in the body it is, and hence what sort of cell to become. A gene that is turned on in one cell may make a product that leaves the cell and diffuses through adjacent cells, entering them and turning on genes only when it is present above a certain threshold level. These cells are thus induced into a new fate, and may even generate other morphogens that signal back to the original cell. Over longer distances morphogens may use the active process of signal transduction. Such signalling controls embryogenesis, the building of a body plan from scratch through a series of sequential steps. They also control and maintain adult bodies through feedback processes, and the loss of such feedback because of a mutation can be responsible for the cell proliferation that is seen in cancer. In parallel with this process of building structure, the gene cascade turns on genes that make structural proteins that give each cell the physical properties it needs.It has been suggested that, because biological molecular interactions are intrinsically stochastic, gene networks are the result of cellular processes and not their cause (i.e. cellular Darwinism). However, recent experimental evidence has favored the attractor view of cell fates.