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Agricultural Biotechnology
Agricultural Biotechnology

... Used for dicots Specific for plant types Used for monocots ...
슬라이드 1
슬라이드 1

... ▶ WRKY (pronounced‘worky’) proteins, a large family of transcriptional regulators that has to date only been found in plants. ▶ The WRKY domain is defined by the conserved amino acidsequence WRKYGQK at its N-terminal end, together with a novel zinc-finger-like motif ▶ Many WRKY proteins having a reg ...
DAAM1 antibody - middle region (ARP55131_P050)
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The UL21 gene of herpes simplex virus 1
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CHAPTER 11: Gene Expression
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... polymerase and increases the rate of transcription from the promoter. In other operons, cAMP-CAP binds to a site centered at -42 and again increases the rate of transription. Note that the same regulatory protein (cAMP-CAP) can make different contacts with RNA polymerase at different operons and act ...
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... Fig.1 Effects of target gene overexpression on lycopene production by engineered E. coli . pACLYC04 contains the Erwinia herbicola crtE , crtB and crtI genes necessary for lycopene biosynthesis in E. coli . pBAD24 were used as vectors for dxs , idi , appY , rpoS , yjiD and ycgW expression. dxs : enc ...
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... a. chewing on willowbark to lesson tooth pain b. grinding up the pancreas from livestock to obtain the protein insulin for treatment of diabetes. c. Use of the foxglove plant to make digitalis, a drug used regulate an irregular heart rate. Some products are produced in such small amount that it is i ...
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pNZ:vig Vector information: IRES

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Outline
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... Test it in cells • RBP and GBP involved in signal transduction pathways at the gene levels • presence of ligand to the above mentioned receptors will cause the upregulation of b-galactosidase gene expression. • The RBP and GBP were replaced with the designed TNT and L-lactate receptors and tested ...
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... complementation with a defective form of beta-galactosidase encoded by host (mutation lacZDM15). In the presence of IPTG, bacteria synthesize both fragments of the enzyme and form blue colonies on media with X-Gal. Insertion of DNA into the MCS located within the lacZ gene (codons 6-7 of lacZ are re ...
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Eat to Regulate Your Genes?

... gene is a segment of DNA that can be “transcribed” into messenger RNA, which then is (or may be) “translated” into protein. The entire process is broadly known as “gene expression.” However, one of the hottest fields of research in molecular biology over the past decade or two has to do with DNA reg ...
Lecture 1 Introduction to Bioinformatics
Lecture 1 Introduction to Bioinformatics

... RNA Genes •  not all genes encode proteins •  for some genes the end product is RNA –  ribosomal RNA (rRNA), which includes major constituents of ribosomes –  transfer RNAs (tRNAs), which carry amino acids to ribosomes –  micro RNAs (miRNAs), which play an important regulatory role in various plant ...
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PowerPoint

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Hemagglutinin Protein (HA1 Subunit) (His Tag)
Hemagglutinin Protein (HA1 Subunit) (His Tag)

... (ABB90704.1) (Met1-Arg349), termed as HA1, was expressed with a Cterminal polyhistidine tag. ...
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Expression vector

An expression vector, otherwise known as an expression construct, is usually a plasmid or virus designed for protein expression in cells. The vector is used to introduce a specific gene into a target cell, and can commandeer the cell's mechanism for protein synthesis to produce the protein encoded by the gene. Expression vectors are the basic tools in biotechnology for the production of proteins.The plasmid is engineered to contain regulatory sequences that act as enhancer and promoter regions and lead to efficient transcription of the gene carried on the expression vector. The goal of a well-designed expression vector is the production of protein, and this may be achieve by the production of significant amount of stable messenger RNA, which can then be translated into protein. The protein may be expressed constitutively, or induced when necessary using an inducer. Escherichia coli is commonly used as the host for protein expression, other cell types however may also be used. An example of the use of expression vector is the production of insulin which is used for medical treatments of diabetes.
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