Trans-activation and DNA-binding properties of
... SoCM specifically competed the Sox-18-SoCM complex, none of the non-HMG-box binding sites had any effect (Fig. 1 A). Several other Sox-like DNA motifs (specifically AACAATC, GACAAAG, TACAATC, AACAAAC and CACAATTG) were also unable to effectively compete the Sox-18-SoCM complex (data not shown). We t ...
... SoCM specifically competed the Sox-18-SoCM complex, none of the non-HMG-box binding sites had any effect (Fig. 1 A). Several other Sox-like DNA motifs (specifically AACAATC, GACAAAG, TACAATC, AACAAAC and CACAATTG) were also unable to effectively compete the Sox-18-SoCM complex (data not shown). We t ...
Chapter 11
... drugs, or other molecules. They are usually 15-40 nucleotides long, have highly organized secondary and tertiary structures, and bind with high affinity. The advantages are their high specificity, relative ease of production, low or no immunogenicity, and long-term stability. Aptamers are typi ...
... drugs, or other molecules. They are usually 15-40 nucleotides long, have highly organized secondary and tertiary structures, and bind with high affinity. The advantages are their high specificity, relative ease of production, low or no immunogenicity, and long-term stability. Aptamers are typi ...
Gene Expression
... genetic makeup of a cell, organism or species. Though most of the genome is non-coding, the coding regions (“genes”) are what primarily determine the traits of organisms. Triplet codes found in a gene code for amino acids. The genetic code is considered to be universal. Structural and functional dif ...
... genetic makeup of a cell, organism or species. Though most of the genome is non-coding, the coding regions (“genes”) are what primarily determine the traits of organisms. Triplet codes found in a gene code for amino acids. The genetic code is considered to be universal. Structural and functional dif ...
Glycoengineering For Therapeutic Proteins
... GLYCOSYLATION PATTERNS OF ERYTHROPOEITN(EPO) CORRELATES WITH T1/2 ...
... GLYCOSYLATION PATTERNS OF ERYTHROPOEITN(EPO) CORRELATES WITH T1/2 ...
w12-proteins
... The direct detection and quantification of the proteins in a biological system o Can also assay protein “states” [post-translational modifications (PTMs), e.g. phosphorylation] o Provides high-confidence detection of proteins/validation of putative coding genes o Provides more accurate protein abu ...
... The direct detection and quantification of the proteins in a biological system o Can also assay protein “states” [post-translational modifications (PTMs), e.g. phosphorylation] o Provides high-confidence detection of proteins/validation of putative coding genes o Provides more accurate protein abu ...
chap-4 - Workforce3One
... – Permits purification by nickel affinity chromatography – His tag can be removed using enzyme enterokinase without damage to the protein product ...
... – Permits purification by nickel affinity chromatography – His tag can be removed using enzyme enterokinase without damage to the protein product ...
Cloning Vector
... Can be useful when proteins are rare cellular components or difficult to isolate ...
... Can be useful when proteins are rare cellular components or difficult to isolate ...
PPT
... all genes are included into one cluster. In the case of divisive clustering, the whole set of genes is considered as a single cluster and is broken down iteratively into sub-clusters with similar expression profiles until each cluster contains only one gene. This information can be represented as a ...
... all genes are included into one cluster. In the case of divisive clustering, the whole set of genes is considered as a single cluster and is broken down iteratively into sub-clusters with similar expression profiles until each cluster contains only one gene. This information can be represented as a ...
Lecture
... cDNA can then be used as a template in PCR, using specific primers for gene of interest (RTPCR) cDNA could be cloned to form a cDNA library cDNA can also be stored for future experiments—more stable than original RNA ...
... cDNA can then be used as a template in PCR, using specific primers for gene of interest (RTPCR) cDNA could be cloned to form a cDNA library cDNA can also be stored for future experiments—more stable than original RNA ...
IntroNetworksandGenes
... proteins, the rest includes RNA coding, promoter, terminators etc. In contrast, the Human genome: 3,000,000,000 base pairs and about 25,000 genes. Only 2% of the Human genome codes for proteins. The rest is……RNA regulatory network? Human genes are also segmented into Exon and Introns, with alternati ...
... proteins, the rest includes RNA coding, promoter, terminators etc. In contrast, the Human genome: 3,000,000,000 base pairs and about 25,000 genes. Only 2% of the Human genome codes for proteins. The rest is……RNA regulatory network? Human genes are also segmented into Exon and Introns, with alternati ...
energy currency for cell - Hermantown Community Schools
... different from each other. • The R groups between the different amino acids help create the proteins shape. • Folds and bonds form creating distinct protein shapes ...
... different from each other. • The R groups between the different amino acids help create the proteins shape. • Folds and bonds form creating distinct protein shapes ...
The gene Q13L coding for the Capripoxvirus group specific
... The gene Q13L coding for the Capripoxvirus group specific structural protein P32 was expressed in Escherichia coli using plasmid pGEX-2T as a fusion protein with glutathione-s-transferase and purified on glutathione sepharose affinity chromatography column. The protein was then employed for diagnosi ...
... The gene Q13L coding for the Capripoxvirus group specific structural protein P32 was expressed in Escherichia coli using plasmid pGEX-2T as a fusion protein with glutathione-s-transferase and purified on glutathione sepharose affinity chromatography column. The protein was then employed for diagnosi ...
Database Modeling in Bioinformatics
... • Manual annotation is best -especially if Medline number attached (biochemical evidence) • InterPro good, assuming protein hit is true and should hit all signatures in an entry • EC numbers good, but need mapping of protein to these, so may be extra step • SWISS-PROT keywords fine, but automatic an ...
... • Manual annotation is best -especially if Medline number attached (biochemical evidence) • InterPro good, assuming protein hit is true and should hit all signatures in an entry • EC numbers good, but need mapping of protein to these, so may be extra step • SWISS-PROT keywords fine, but automatic an ...
Quiz 2
... been successful in disrupting the gene of interest. Do you expect the mutant gene to be larger or smaller than the normal copy? Explain your answer. (5 points) It depends on the type of vector used to generate the mutation and the size of the Neor gene. If the vector contained a large deletion in th ...
... been successful in disrupting the gene of interest. Do you expect the mutant gene to be larger or smaller than the normal copy? Explain your answer. (5 points) It depends on the type of vector used to generate the mutation and the size of the Neor gene. If the vector contained a large deletion in th ...
Molecular Biology - Gene Regulation
... the same proteins. Prokaryotic organisms express the entire DNA they encode in every cell, but not necessarily all at the same time. Proteins are expressed only when they are needed. Eukaryotic organisms express a subset of the DNA that is encoded in any given cell. In each cell type, the type and a ...
... the same proteins. Prokaryotic organisms express the entire DNA they encode in every cell, but not necessarily all at the same time. Proteins are expressed only when they are needed. Eukaryotic organisms express a subset of the DNA that is encoded in any given cell. In each cell type, the type and a ...
Enterokinase (E4906)
... an 800 amino acid heavy chain and a 235 amino acid light chain. This product (E4906) is supplied as the 28 kDa catalytic light chain. Enterokinase is a highly specific serine protease and, in vivo, enterokinase specifically hydrolyzes the lysineisoleucine bond of the activation peptide in trypsinoge ...
... an 800 amino acid heavy chain and a 235 amino acid light chain. This product (E4906) is supplied as the 28 kDa catalytic light chain. Enterokinase is a highly specific serine protease and, in vivo, enterokinase specifically hydrolyzes the lysineisoleucine bond of the activation peptide in trypsinoge ...
Gene Regulation - Marblehead High School
... protein your body needs? Are they all making every possible protein that you need all of the time? How does each cell know what protein to make and when to make it? ...
... protein your body needs? Are they all making every possible protein that you need all of the time? How does each cell know what protein to make and when to make it? ...
Influenza A H3N2 (A/X-31) Hemagglutinin / HA Protein (His Tag)
... Protein Construction: A DNA sequence encoding the Influenza A virus (A/X-31(H3N2)) hemagglutinin (P03438.2) (Met1-Trp530), termed as HA, was expressed with a polyhistidine tag at the C-terminus. ...
... Protein Construction: A DNA sequence encoding the Influenza A virus (A/X-31(H3N2)) hemagglutinin (P03438.2) (Met1-Trp530), termed as HA, was expressed with a polyhistidine tag at the C-terminus. ...
Cell-Free Test Expression Information Sheet
... We will need as much information on the protein/template as you can provide for us to make good decisions regarding the selected screening/expression conditions. Please complete a separate fact sheet for each target. ...
... We will need as much information on the protein/template as you can provide for us to make good decisions regarding the selected screening/expression conditions. Please complete a separate fact sheet for each target. ...
Lecture 1
... An overview of the mechanisms that can be used in regulation.The product of gene A is an enzyme A, which in this case is synthesised constitutively and carries out its reaction. Enzyme B is also synthesised constitutively but its activity can be inhibited. The synthesis of the product of gene C can ...
... An overview of the mechanisms that can be used in regulation.The product of gene A is an enzyme A, which in this case is synthesised constitutively and carries out its reaction. Enzyme B is also synthesised constitutively but its activity can be inhibited. The synthesis of the product of gene C can ...
Word file (24 KB )
... of approximately 1.8 kb was ligated into the Nhe I and Sal I sites of the pN-Z-TK2 targeting vector, containing a promoter-less lacZ and neomycin-resistance gene under control of the RNA polymerase II promoter (gift from R. Palmiter). A 7-kb Bam HI-Xho I fragment was used as a long arm. The targetin ...
... of approximately 1.8 kb was ligated into the Nhe I and Sal I sites of the pN-Z-TK2 targeting vector, containing a promoter-less lacZ and neomycin-resistance gene under control of the RNA polymerase II promoter (gift from R. Palmiter). A 7-kb Bam HI-Xho I fragment was used as a long arm. The targetin ...
transcription
... • Primary transcripts associate with a family of polypeptides known as hnRNP proteins • They contain RNA-binding motifs, and Gly-rich domains for protein–protein interactions and RNA transport • hnRNP packaging can also bring together distant regions of the pre-mRNA and therefore assist splicesite p ...
... • Primary transcripts associate with a family of polypeptides known as hnRNP proteins • They contain RNA-binding motifs, and Gly-rich domains for protein–protein interactions and RNA transport • hnRNP packaging can also bring together distant regions of the pre-mRNA and therefore assist splicesite p ...
Mammalian two-hybrid (M2H) and co-immunoprecipitation (co
... by SDS-PAGE (4-12% gradient gels, Bio-Rad) and transferred to nitrocellulose ...
... by SDS-PAGE (4-12% gradient gels, Bio-Rad) and transferred to nitrocellulose ...