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N8113S N8113S
N8113S N8113S

... Description: The vector pMAL-p5G is designed to produce maltose-binding protein (MBP) fusions, where the protein of interest can be cleaved from MBP with the specific protease Genenase™ I (NEB #P8075). MBP fusions made with this vector include an N-terminal signal sequence, so the fusion protein is ...
HNF4a Network - University of Wisconsin–Madison
HNF4a Network - University of Wisconsin–Madison

... Hepatic nuclear factor 4 alpha C/EBP b CCAAT/enhancer binding protein beta ...
GoMap
GoMap

... databases) and GO terms with evidence -link to BLAST search results • Have GO term assignment linked to InterProScan, in the meantime, link hits to GO via mapping file • Use EC number mappings if your protein hits an enzyme ...
1: How is ribonucleic acid like DNA
1: How is ribonucleic acid like DNA

... 19: What is translation? By what is the mRNA message read in order to translate the proteins? ...
DNA Code problerm
DNA Code problerm

... B. instability of the DNA molecule C. the ability of the same tRNA anticodon to recognize different codons D. the high mutability of certain genes 9. If you wanted to block transcription of a group of functionally related genes in a prokaryote, you could place an obstacle A. upstream of both the pro ...
RESTRICTION ENZYMES AND VECTORS
RESTRICTION ENZYMES AND VECTORS

... immediately outside their recognition sequences .they require Mg ion for cleavage. Type 3 Restriction endonucleases are intermediate b/w the Type1&Type2 enzymes ; they cleave DNA in the immediate vicinity of their recognition sites, e.g, Ecop1 , Ecop15, Hinf3 etc. ...
Genetic Engineering
Genetic Engineering

... molecules gene (X) of interest (the target DNA) is inserted into a plasmid vector . The target DNA may be a single fragment isolated from an agarose gel , or a mixture of many fragments from, for example, genomic DNA . If the target has been prepared by digestion with EcoRI, then the fragment can be ...
Wrap up Genes and Expression
Wrap up Genes and Expression

... (d). Finally, introns can be retained in the mRNA and become translated. ...
Function of Sequence Elements (PowerPoint) Madison 2006
Function of Sequence Elements (PowerPoint) Madison 2006

... Reintroduce the modular nature of gene expression or gene Regulation. Regulation can be separated from the structural gene And that will emphasize where that protein is made is based on the enhancer, and which proteins is made depends on the structural Gene. ...
Puredown Protein A/G-Agarose Conjugate
Puredown Protein A/G-Agarose Conjugate

... solution using an antibody that specifically binds to that particular protein. This process can be used to isolate and concentrate a particular protein from a sample containing many thousands of different proteins. Immunoprecipitation requires that the antibody be coupled to a solid substrate at som ...
60% 74% - Ingredion
60% 74% - Ingredion

... Overall, there is a strong interest among consumers in protein and the health and wellness benefits it provides. Consumers want the pleasure of real food with a clear single-minded benefit like enhanced protein content. Wouldn’t you like to help consumers and food manufacturers to easily incorporate ...
REGULATION OF GENES INVOLVED IN LIPID CATABOLISM
REGULATION OF GENES INVOLVED IN LIPID CATABOLISM

... all the cycle. In Arabidopsis the ACX family is composed of at least four isozymes with distinct but partially overlapping substrate specificities. AtACX1 is particularly active medium- to long-chain saturated acyl-CoA; AtACX2 prefers long-chain unsaturated acylCoAs; AtACX3 preferred substrates are ...
10/7
10/7

... The level of this mRNA increases after plants are exposed to light. ...
Characterization of the protein recognized by the monoclonal
Characterization of the protein recognized by the monoclonal

... The objective of this study was to characterize low molecular weight proteins of B. burgdorferi sensu lato. Our main focus was a protein around 12 kDa, that is reactive with D6, a monoclonal antibody specific for B. garinii isolates. ...
Role of cystinosin in vesicular trafficking and membrane fusion
Role of cystinosin in vesicular trafficking and membrane fusion

... (collaboration with Hybrigenics). We identified different putative partners and focused on two of them: Vps39 and Snf8 (the murine homolog of Vps22) both being implicated in membrane fusion and trafficking respectively (Caplan et al, 2001; Progida et al, 2006). As we have reported previously, we wan ...
Recombinant Human Olfactory Marker Protein ab114419 Product datasheet 1 Image
Recombinant Human Olfactory Marker Protein ab114419 Product datasheet 1 Image

... Protein concentration is above or equal to 0.05 mg/ml. Best used within three months from the date of receipt. ...
What is latency? - California State University, Fullerton
What is latency? - California State University, Fullerton

... • TAT - transactivator of transcription needed for efficient transcription of HIV • TAT binds to TAR in nascent RNA and lets polymerase elongate • Initially low level of transcription until TAT levels rise • What are possible targets? ...
基因定点整合
基因定点整合

... transgenes derived from different sources typically integrate at different locations in the plant genome, which may lead to various expression patterns and possible segregation of the transgenes in the offspring. ...
PPT
PPT

... Biology is becoming a more theoretical, ...
Reporter genes
Reporter genes

... Co-transfection of reporter genes Dual Luciferase Assay system - Promega Clone promoter of interest in front of firefly luciferase and use Renilla luciferase as an internal control co-transfect and assay ...
GFP
GFP

... Co-transfection of reporter genes Dual Luciferase Assay system - Promega Clone promoter of interest in front of firefly luciferase and use Renilla luciferase as an internal control co-transfect and assay ...
Gene Section TCL1B (T-cell leukemia/lymphoma 1B) Atlas of Genetics and Cytogenetics
Gene Section TCL1B (T-cell leukemia/lymphoma 1B) Atlas of Genetics and Cytogenetics

... causing a TCRalpha/delta- TCL1A /TCL1B translocation ...
Biology Pre-Learning Check
Biology Pre-Learning Check

... We will also study the process where RNA is used to make proteins. Specifically, we will study each part (transcription and translation), the steps involved and the enzymes involved. We will also look more specifically at mutations; how they can occur and what effects they might have. Pages in the b ...
Biochemistry Jeopardy C.P. Bio.
Biochemistry Jeopardy C.P. Bio.

... Fats = 9 C/g Carbs = 4 C/g Protein = 4 C/g ...
Document
Document

... GLUCOSE-LACTATE METABOLIC COOPERATION ...
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Expression vector

An expression vector, otherwise known as an expression construct, is usually a plasmid or virus designed for protein expression in cells. The vector is used to introduce a specific gene into a target cell, and can commandeer the cell's mechanism for protein synthesis to produce the protein encoded by the gene. Expression vectors are the basic tools in biotechnology for the production of proteins.The plasmid is engineered to contain regulatory sequences that act as enhancer and promoter regions and lead to efficient transcription of the gene carried on the expression vector. The goal of a well-designed expression vector is the production of protein, and this may be achieve by the production of significant amount of stable messenger RNA, which can then be translated into protein. The protein may be expressed constitutively, or induced when necessary using an inducer. Escherichia coli is commonly used as the host for protein expression, other cell types however may also be used. An example of the use of expression vector is the production of insulin which is used for medical treatments of diabetes.
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